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The map label for this gene is malI [H]

Identifier: 59712327

GI number: 59712327

Start: 1937643

End: 1938665

Strand: Direct

Name: malI [H]

Synonym: VF_1720

Alternate gene names: 59712327

Gene position: 1937643-1938665 (Clockwise)

Preceding gene: 59712324

Following gene: 172087722

Centisome position: 66.87

GC content: 39.1

Gene sequence:

>1023_bases
ATGATAAATAAAAAAGCAAATATAAAGGATGTCGCTGAACGTGCTGGGGTTTCTGTAACCACGGTTTCGATGGTGCTAAG
CGAAAAAGGGCGTATATCTTCTGATACGATTGATAAAGTGAATCAAGCCGTACAAGATTTGGGTTATATAAAAAACCGTA
CAGCCTCTAACCTACGCTCACAAAAGTCGACTTTAATAGGCTTAATCTTACGCGACATCAGTGACCCTTATTATGCTGAA
GTTGCCGCTGGAATAAGTGAAGTATTAGAAGAAAAAGGCTATATGCTTTTTCTTGCTCAATCAGGCACAAGCCAAGAAAA
ATTTGACCAATGTTTAGCAACCATGATTGCTCAAGATATTGGTGGGATCATTTTCTGTCCTATTCGTGATTCAGAGCTCC
CTAACCTTGATAAAGTGAAAAACGTAGGCCTTCCCATTGTTTGTGTTGCTCGAGCCAAAATAAATCAACCCATCGATTTT
GTTGGTCCTGATAACATGCAAGCAGCTAAAATTGCCACTGAATATCTCATTAAACAAGGACATCGACAAATTGCTTATGT
TGGTGGACAAAGTGACTCTTTAAGTCGAGCTGAGCGTTTAGGTGGCTATTGCAGCACCCTAATGCAGTTTGGCTTACCAT
TTAAATCTGAATGGGTTGTTGAATGCGATAAAAGCCAAACTGCATCGGCAGAAGCGGTAAGTCAATTAATTAATAAAAAC
CCTAAGATTACTGCGATTCTTTGTCACTATTCATCGACAGCTTTTGGTGCAGTATACGGCGTAAATCGAGCTAATCGCAG
TGTAGGTAAAGATAACTACATTGGTCAACAAGTTGCTATTATTGGATTTGATGATGTACCTGAAGCTGAACTTATTCAAC
CATCACTTACTTTTGTATCCTCACCGATACGAGATATTGGTCGCCAAGCAGGTCATCGATTAATAAATCAAATCAATGAT
AAAGGCAGTGTGCCTCAAAGTTTAATCTTAAGCCCTGAGTTAATTGCTCGTAATTCTGCATAA

Upstream 100 bases:

>100_bases
ATATAATTTTTCAATTTATATACAAATGCTATAGAATGAGGAATCTAAAGAAGGTTTTGTTAGGTGAAACGTTTTATCAA
AGACAACTAAAAGAAGAATC

Downstream 100 bases:

>100_bases
ATAAAAAAGATAACTGAGTTATTAGTGGGAATAATATTAATAACCAAACAAATACATTGATATTAATGTAATAACAAACT
AACGTGAAATCTCAACTTCA

Product: DNA-binding transcriptional repressor MalI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MINKKANIKDVAERAGVSVTTVSMVLSEKGRISSDTIDKVNQAVQDLGYIKNRTASNLRSQKSTLIGLILRDISDPYYAE
VAAGISEVLEEKGYMLFLAQSGTSQEKFDQCLATMIAQDIGGIIFCPIRDSELPNLDKVKNVGLPIVCVARAKINQPIDF
VGPDNMQAAKIATEYLIKQGHRQIAYVGGQSDSLSRAERLGGYCSTLMQFGLPFKSEWVVECDKSQTASAEAVSQLINKN
PKITAILCHYSSTAFGAVYGVNRANRSVGKDNYIGQQVAIIGFDDVPEAELIQPSLTFVSSPIRDIGRQAGHRLINQIND
KGSVPQSLILSPELIARNSA

Sequences:

>Translated_340_residues
MINKKANIKDVAERAGVSVTTVSMVLSEKGRISSDTIDKVNQAVQDLGYIKNRTASNLRSQKSTLIGLILRDISDPYYAE
VAAGISEVLEEKGYMLFLAQSGTSQEKFDQCLATMIAQDIGGIIFCPIRDSELPNLDKVKNVGLPIVCVARAKINQPIDF
VGPDNMQAAKIATEYLIKQGHRQIAYVGGQSDSLSRAERLGGYCSTLMQFGLPFKSEWVVECDKSQTASAEAVSQLINKN
PKITAILCHYSSTAFGAVYGVNRANRSVGKDNYIGQQVAIIGFDDVPEAELIQPSLTFVSSPIRDIGRQAGHRLINQIND
KGSVPQSLILSPELIARNSA
>Mature_340_residues
MINKKANIKDVAERAGVSVTTVSMVLSEKGRISSDTIDKVNQAVQDLGYIKNRTASNLRSQKSTLIGLILRDISDPYYAE
VAAGISEVLEEKGYMLFLAQSGTSQEKFDQCLATMIAQDIGGIIFCPIRDSELPNLDKVKNVGLPIVCVARAKINQPIDF
VGPDNMQAAKIATEYLIKQGHRQIAYVGGQSDSLSRAERLGGYCSTLMQFGLPFKSEWVVECDKSQTASAEAVSQLINKN
PKITAILCHYSSTAFGAVYGVNRANRSVGKDNYIGQQVAIIGFDDVPEAELIQPSLTFVSSPIRDIGRQAGHRLINQIND
KGSVPQSLILSPELIARNSA

Specific function: Repressor for the malX and malY genes. Also regulates its own expression. Binds maltose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787906, Length=336, Percent_Identity=43.1547619047619, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1787948, Length=340, Percent_Identity=28.2352941176471, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=27.8443113772455, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1790194, Length=336, Percent_Identity=27.0833333333333, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1789068, Length=336, Percent_Identity=30.0595238095238, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI48994940, Length=332, Percent_Identity=29.8192771084337, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789202, Length=338, Percent_Identity=29.2899408284024, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1788474, Length=308, Percent_Identity=28.8961038961039, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787580, Length=340, Percent_Identity=26.1764705882353, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790715, Length=323, Percent_Identity=24.1486068111455, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1786540, Length=341, Percent_Identity=26.3929618768328, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1786268, Length=312, Percent_Identity=25.3205128205128, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790689, Length=339, Percent_Identity=24.7787610619469, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36914; Mature: 36914

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKKANIKDVAERAGVSVTTVSMVLSEKGRISSDTIDKVNQAVQDLGYIKNRTASNLRS
CCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKSTLIGLILRDISDPYYAEVAAGISEVLEEKGYMLFLAQSGTSQEKFDQCLATMIAQDI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHC
GGIIFCPIRDSELPNLDKVKNVGLPIVCVARAKINQPIDFVGPDNMQAAKIATEYLIKQG
CCEEEEECCCCCCCCHHHHHHCCCCEEEEEEHHCCCCCCCCCCCCCHHHHHHHHHHHHCC
HRQIAYVGGQSDSLSRAERLGGYCSTLMQFGLPFKSEWVVECDKSQTASAEAVSQLINKN
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHCCC
PKITAILCHYSSTAFGAVYGVNRANRSVGKDNYIGQQVAIIGFDDVPEAELIQPSLTFVS
CCEEEEEEECCCCHHHHHHCCCHHHHCCCCCCCCCCEEEEEECCCCCCHHHCCHHHHHHH
SPIRDIGRQAGHRLINQINDKGSVPQSLILSPELIARNSA
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHCCCC
>Mature Secondary Structure
MINKKANIKDVAERAGVSVTTVSMVLSEKGRISSDTIDKVNQAVQDLGYIKNRTASNLRS
CCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKSTLIGLILRDISDPYYAEVAAGISEVLEEKGYMLFLAQSGTSQEKFDQCLATMIAQDI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHC
GGIIFCPIRDSELPNLDKVKNVGLPIVCVARAKINQPIDFVGPDNMQAAKIATEYLIKQG
CCEEEEECCCCCCCCHHHHHHCCCCEEEEEEHHCCCCCCCCCCCCCHHHHHHHHHHHHCC
HRQIAYVGGQSDSLSRAERLGGYCSTLMQFGLPFKSEWVVECDKSQTASAEAVSQLINKN
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHCCC
PKITAILCHYSSTAFGAVYGVNRANRSVGKDNYIGQQVAIIGFDDVPEAELIQPSLTFVS
CCEEEEEEECCCCHHHHHHCCCHHHHCCCCCCCCCCEEEEEECCCCCCHHHCCHHHHHHH
SPIRDIGRQAGHRLINQINDKGSVPQSLILSPELIARNSA
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670898; 1856179; 9097039; 9278503 [H]