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The map label for this gene is mnmC

Identifier: 59712308

GI number: 59712308

Start: 1917497

End: 1919539

Strand: Direct

Name: mnmC

Synonym: VF_1701

Alternate gene names: 59712308

Gene position: 1917497-1919539 (Clockwise)

Preceding gene: 59712297

Following gene: 59712311

Centisome position: 66.18

GC content: 40.04

Gene sequence:

>2043_bases
ATGTCACAAAACCACATTTTACCACAAAACAGCATCACTAATGCAGTCCTTGAATGGAATGAGTCTGGAACACCCGTCTC
AAATGATTTTGATGACGTGTATTTTTCTAACGATAATGGGTTAGAAGAAACACGTTATGTTTTCTTACAACAAAATCATC
TTCCACAAAGATGGCAAGAATATGACCAGCGTCGTTTTGTGATTGGTGAAACAGGATTTGGGACTGGATTAAACTTTCTT
GCTGTATGGCAATGGTTCAAAGAGTTCCGCTCACAATATCCTGATGCTCCTTTAAAAGAGCTTCACTTTGTTAGCTTTGA
AAAATTCCCAGTTACTAAATCTGATTTAATTAAAGCTCATCAAGCGTGGCCAGAATTGGCTCAATTTGCTGAACAGTTAC
AAGAACACTACCCAGCTGCCGTACCTGATTGCCATCGTTTAGTACTAGAAGACGGCATGATAACACTTGATCTCTGGTTT
GGTGACATAAAAGATTGCATGCCTCAAATCTGGATGGACGATAAAGGGTTAATTGATGCATGGTTCCTCGATGGTTTTGC
ACCAAGTAAAAACCCTGAGATGTGGAATCAAACTCTGTTTAATAACATGGCAAGCCTAGCTAAAAAAGGCTGCACCTGTG
CAACGTTTACTGCAGCGGGATTTGTTCGTAGAGGCCTAATTGAAGCTGGCTTTGATATGAAGAAAGTCAAAGGCTTTGGG
CATAAACGCGAAATGATTGCAGGCACATTAACGGAGCGTACAACCAAAGCAAACCACGAAGTATGGTATGCCCGCTCAAC
CAAAGAAAACATCACTGACGTTGCTATTATCGGGGGAGGTGTTGCTAGTGCTGCATTAGCGACAACCTTAATTAGACGTG
GTGTAAAAGTGAGTGTTTACTGCAAAGATGAGAAATCAGCACAAGGGGCATCAGGTAACAAACAAGGTGCCGTATATCCA
CTGCTTAATGAGAAATTCAATTCCCTTTCTCGTTTTTTTGCTCCAGGATTTATTTTTGCTCGTCAATTCATCGACCAAGC
AGCCAAACACGTAGAATTTGATCATGATTGGTGTGGTGTTACCCAGTTAAAATGGGATGAAAAATCCGCCAATAAGCTAA
ATAAAATGCTTGAGGGAAATTTCCCTAATGAACTTGTTTCTTCTTTTGATATTGATAAAACCAATCAAATGGTTGGATTA
CCGATTAATATGGAAAGTGTCCACTACTCATTAGGTGGCTGGTTATGTCCAAAACAATTAACTCGCGGATTATTTGAACA
TTTATCAAATAACCCACTGTTCACTCTGCATTGTGATTCAGAAATAATCGCATTAACACAGAACGAAGAACAACAATGGC
TTCTATCAACAGATAGCAACGCTTACCAACATCAAGCCGTTGTGGTTGCAAATGGGCACAGATTTACTGATTTTGAGCAA
ACTAAAGACATTCCAGCAACGCCTGTTCGTGGACAAGTCAGTCATATTCCAACGACAGAATCACTAAAAAATCTGAAAAC
CGTATTATGTTATGACGGTTACTTAACGCCTGAAAACAGCAAACATCAGACTCATTGTATTGGTGCTAGTTATGATCGTC
GTGATTTAGATCTTGCATTTAAAGAGTCAGATCAAATCGAAAATGGCGAGAGATTACGAAAATGTGTTCCAAACGAGGTA
TGGCCAAACGACGTTGATACTAGCGATAACCAAGCTCGTGTAGGCATTCGATGTGCTAGTCGTGATCACCTACCATTTAT
CGGAAACGTGGTACGTTTTGAAGAGATGCAAGAAGAATACAAAAACATCTATAAGAAACGCCACTGGTTACGTGAAGCTA
AAGATATCCCTGTTTATGAAGGTTTATTCTGCATGTTAACACTGGGATCAAGAGGATTGAGCTCTGCACCATTATTGGCT
GAAGCCTTAGCATCACAAATTATGGGTGACCCAATTCCACTACCAAATTCAGTGCTTGAAGGATTACACCCAGGAAGATT
ATGGGTGAGAAGATTACTGAAAGGGAAACCGTTGGATATTTAA

Upstream 100 bases:

>100_bases
TAAATCTTGGTAGATAATAGCGAAGATAGCACAACAAAGTGGTCAGCTTTCCTATAAGTCGTTAAAATCCGACGTAGATT
ATCTTTAATGGTACAAATTT

Downstream 100 bases:

>100_bases
GGAAAGTAAGAGCAGGGACTAGGCCGGTCGCTAGCTCCCTTTCTGGTTTCAGGGGGCTAGTTGTAAGCGATGAACTCACA
ACAAACTCCCCTGAATCCTT

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 680; Mature: 679

Protein sequence:

>680_residues
MSQNHILPQNSITNAVLEWNESGTPVSNDFDDVYFSNDNGLEETRYVFLQQNHLPQRWQEYDQRRFVIGETGFGTGLNFL
AVWQWFKEFRSQYPDAPLKELHFVSFEKFPVTKSDLIKAHQAWPELAQFAEQLQEHYPAAVPDCHRLVLEDGMITLDLWF
GDIKDCMPQIWMDDKGLIDAWFLDGFAPSKNPEMWNQTLFNNMASLAKKGCTCATFTAAGFVRRGLIEAGFDMKKVKGFG
HKREMIAGTLTERTTKANHEVWYARSTKENITDVAIIGGGVASAALATTLIRRGVKVSVYCKDEKSAQGASGNKQGAVYP
LLNEKFNSLSRFFAPGFIFARQFIDQAAKHVEFDHDWCGVTQLKWDEKSANKLNKMLEGNFPNELVSSFDIDKTNQMVGL
PINMESVHYSLGGWLCPKQLTRGLFEHLSNNPLFTLHCDSEIIALTQNEEQQWLLSTDSNAYQHQAVVVANGHRFTDFEQ
TKDIPATPVRGQVSHIPTTESLKNLKTVLCYDGYLTPENSKHQTHCIGASYDRRDLDLAFKESDQIENGERLRKCVPNEV
WPNDVDTSDNQARVGIRCASRDHLPFIGNVVRFEEMQEEYKNIYKKRHWLREAKDIPVYEGLFCMLTLGSRGLSSAPLLA
EALASQIMGDPIPLPNSVLEGLHPGRLWVRRLLKGKPLDI

Sequences:

>Translated_680_residues
MSQNHILPQNSITNAVLEWNESGTPVSNDFDDVYFSNDNGLEETRYVFLQQNHLPQRWQEYDQRRFVIGETGFGTGLNFL
AVWQWFKEFRSQYPDAPLKELHFVSFEKFPVTKSDLIKAHQAWPELAQFAEQLQEHYPAAVPDCHRLVLEDGMITLDLWF
GDIKDCMPQIWMDDKGLIDAWFLDGFAPSKNPEMWNQTLFNNMASLAKKGCTCATFTAAGFVRRGLIEAGFDMKKVKGFG
HKREMIAGTLTERTTKANHEVWYARSTKENITDVAIIGGGVASAALATTLIRRGVKVSVYCKDEKSAQGASGNKQGAVYP
LLNEKFNSLSRFFAPGFIFARQFIDQAAKHVEFDHDWCGVTQLKWDEKSANKLNKMLEGNFPNELVSSFDIDKTNQMVGL
PINMESVHYSLGGWLCPKQLTRGLFEHLSNNPLFTLHCDSEIIALTQNEEQQWLLSTDSNAYQHQAVVVANGHRFTDFEQ
TKDIPATPVRGQVSHIPTTESLKNLKTVLCYDGYLTPENSKHQTHCIGASYDRRDLDLAFKESDQIENGERLRKCVPNEV
WPNDVDTSDNQARVGIRCASRDHLPFIGNVVRFEEMQEEYKNIYKKRHWLREAKDIPVYEGLFCMLTLGSRGLSSAPLLA
EALASQIMGDPIPLPNSVLEGLHPGRLWVRRLLKGKPLDI
>Mature_679_residues
SQNHILPQNSITNAVLEWNESGTPVSNDFDDVYFSNDNGLEETRYVFLQQNHLPQRWQEYDQRRFVIGETGFGTGLNFLA
VWQWFKEFRSQYPDAPLKELHFVSFEKFPVTKSDLIKAHQAWPELAQFAEQLQEHYPAAVPDCHRLVLEDGMITLDLWFG
DIKDCMPQIWMDDKGLIDAWFLDGFAPSKNPEMWNQTLFNNMASLAKKGCTCATFTAAGFVRRGLIEAGFDMKKVKGFGH
KREMIAGTLTERTTKANHEVWYARSTKENITDVAIIGGGVASAALATTLIRRGVKVSVYCKDEKSAQGASGNKQGAVYPL
LNEKFNSLSRFFAPGFIFARQFIDQAAKHVEFDHDWCGVTQLKWDEKSANKLNKMLEGNFPNELVSSFDIDKTNQMVGLP
INMESVHYSLGGWLCPKQLTRGLFEHLSNNPLFTLHCDSEIIALTQNEEQQWLLSTDSNAYQHQAVVVANGHRFTDFEQT
KDIPATPVRGQVSHIPTTESLKNLKTVLCYDGYLTPENSKHQTHCIGASYDRRDLDLAFKESDQIENGERLRKCVPNEVW
PNDVDTSDNQARVGIRCASRDHLPFIGNVVRFEEMQEEYKNIYKKRHWLREAKDIPVYEGLFCMLTLGSRGLSSAPLLAE
ALASQIMGDPIPLPNSVLEGLHPGRLWVRRLLKGKPLDI

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=668, Percent_Identity=52.9940119760479, Blast_Score=697, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_VIBF1 (Q5E450)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_205084.1
- ProteinModelPortal:   Q5E450
- SMR:   Q5E450
- STRING:   Q5E450
- GeneID:   3278905
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1701
- NMPDR:   fig|312309.3.peg.1701
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- PhylomeDB:   Q5E450
- ProtClustDB:   PRK01747
- BioCyc:   VFIS312309:VF1701-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 77237; Mature: 77106

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNHILPQNSITNAVLEWNESGTPVSNDFDDVYFSNDNGLEETRYVFLQQNHLPQRWQE
CCCCCCCCCCHHHHHHEEECCCCCCCCCCCCCEEECCCCCCCHHEEEEEECCCCCHHHHH
YDQRRFVIGETGFGTGLNFLAVWQWFKEFRSQYPDAPLKELHFVSFEKFPVTKSDLIKAH
HCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHEECCCCCCHHHHHHHH
QAWPELAQFAEQLQEHYPAAVPDCHRLVLEDGMITLDLWFGDIKDCMPQIWMDDKGLIDA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEEEEHHHHHHHHHHHCCCCCCCEEH
WFLDGFAPSKNPEMWNQTLFNNMASLAKKGCTCATFTAAGFVRRGLIEAGFDMKKVKGFG
HHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCHHHHHCCC
HKREMIAGTLTERTTKANHEVWYARSTKENITDVAIIGGGVASAALATTLIRRGVKVSVY
CHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEE
CKDEKSAQGASGNKQGAVYPLLNEKFNSLSRFFAPGFIFARQFIDQAAKHVEFDHDWCGV
ECCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCC
TQLKWDEKSANKLNKMLEGNFPNELVSSFDIDKTNQMVGLPINMESVHYSLGGWLCPKQL
CEEECCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEECCCCHHHHHHHCCCEECHHHH
TRGLFEHLSNNPLFTLHCDSEIIALTQNEEQQWLLSTDSNAYQHQAVVVANGHRFTDFEQ
HHHHHHHHCCCCEEEEECCCEEEEEECCCCCEEEEECCCCCCEEEEEEEECCCCCCCHHH
TKDIPATPVRGQVSHIPTTESLKNLKTVLCYDGYLTPENSKHQTHCIGASYDRRDLDLAF
CCCCCCCCCCCCCCCCCCHHHHHHHHEEEEECCEECCCCCCCCEEEECCCCCCCCCCEEE
KESDQIENGERLRKCVPNEVWPNDVDTSDNQARVGIRCASRDHLPFIGNVVRFEEMQEEY
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
KNIYKKRHWLREAKDIPVYEGLFCMLTLGSRGLSSAPLLAEALASQIMGDPIPLPNSVLE
HHHHHHHHHHHHHCCCCHHHHHHHEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHC
GLHPGRLWVRRLLKGKPLDI
CCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SQNHILPQNSITNAVLEWNESGTPVSNDFDDVYFSNDNGLEETRYVFLQQNHLPQRWQE
CCCCCCCCCHHHHHHEEECCCCCCCCCCCCCEEECCCCCCCHHEEEEEECCCCCHHHHH
YDQRRFVIGETGFGTGLNFLAVWQWFKEFRSQYPDAPLKELHFVSFEKFPVTKSDLIKAH
HCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHEECCCCCCHHHHHHHH
QAWPELAQFAEQLQEHYPAAVPDCHRLVLEDGMITLDLWFGDIKDCMPQIWMDDKGLIDA
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEEEEHHHHHHHHHHHCCCCCCCEEH
WFLDGFAPSKNPEMWNQTLFNNMASLAKKGCTCATFTAAGFVRRGLIEAGFDMKKVKGFG
HHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCHHHHHCCC
HKREMIAGTLTERTTKANHEVWYARSTKENITDVAIIGGGVASAALATTLIRRGVKVSVY
CHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEE
CKDEKSAQGASGNKQGAVYPLLNEKFNSLSRFFAPGFIFARQFIDQAAKHVEFDHDWCGV
ECCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCC
TQLKWDEKSANKLNKMLEGNFPNELVSSFDIDKTNQMVGLPINMESVHYSLGGWLCPKQL
CEEECCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEECCCCHHHHHHHCCCEECHHHH
TRGLFEHLSNNPLFTLHCDSEIIALTQNEEQQWLLSTDSNAYQHQAVVVANGHRFTDFEQ
HHHHHHHHCCCCEEEEECCCEEEEEECCCCCEEEEECCCCCCEEEEEEEECCCCCCCHHH
TKDIPATPVRGQVSHIPTTESLKNLKTVLCYDGYLTPENSKHQTHCIGASYDRRDLDLAF
CCCCCCCCCCCCCCCCCCHHHHHHHHEEEEECCEECCCCCCCCEEEECCCCCCCCCCEEE
KESDQIENGERLRKCVPNEVWPNDVDTSDNQARVGIRCASRDHLPFIGNVVRFEEMQEEY
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
KNIYKKRHWLREAKDIPVYEGLFCMLTLGSRGLSSAPLLAEALASQIMGDPIPLPNSVLE
HHHHHHHHHHHHHCCCCHHHHHHHEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHC
GLHPGRLWVRRLLKGKPLDI
CCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA