Click here to switch to the map view.

The map label for this gene is purF [H]

Identifier: 59712298

GI number: 59712298

Start: 1903587

End: 1905101

Strand: Reverse

Name: purF [H]

Synonym: VF_1691

Alternate gene names: 59712298

Gene position: 1905101-1903587 (Counterclockwise)

Preceding gene: 59712299

Following gene: 59712296

Centisome position: 65.75

GC content: 40.0

Gene sequence:

>1515_bases
ATGTGCGGTATTGTTGGAATCGTGGGCGCAACACCAGTAAACCAGTCAATCTATGACGCTTTAACAGTGCTACAACATCG
TGGCCAAGATGCTGCTGGTATTATTACCATAGAAAGCAATCGTTTCCGTCTGAGAAAGGCGAACGGTTTGGTGAAGGATG
TGTTTGAAACAAAACACATGCAACGCCTACAAGGTAACGTTGGTATTGGACATGTTCGATACCCTACAGCAGGTAGTTCT
AGTGCCTCAGAAGCACAACCTTTCTATGTAAACTCTCCATTTGGTATCACTCTTGCGCATAATGGTAACTTAACCAATGC
AAATGAAATTCGTGAAACGTTATTTGAACAAGAGCGTCGTCATGTAAATACAACCTCTGATTCTGAAGTTTTATTAAATA
TCTTTGCACACCATCTTGATGAATCAAAAAATTACCCGCTAACAAACGATGATATCTTTAAAACTGTAGCGAAAGTTCAT
GAACAAATTCGTGGTGCTTATGCAGTAACGGCAATGATCATTGGCCATGGCATGGTGGCATTTCGAGATCCAAACGGCAT
TCGCCCATTATGTTTAGGTAAGCGTGAAATTGAAGGTCGCACAGAATATATGGTGGCTTCAGAGTCAGTCGCTTTGGATG
CAGTAGGCTTTGATTTCGTTCGTGATGTTGCACCGGGTGAAGCGATTTATGCTCCATTTGAAGGGGGTTTATATACTCAA
CAATGTGCGCATGGACCAAAGTTAAATCCATGTATTTTTGAATTTGTTTATTTTGCTCGTCCAGATTCGTTTATTGATAA
AATTTCGGTATACAGTGCACGAGTTGAAATGGGTAAGCGTTTAGGTGAGAAAATTAAGCGTGAGTGGGATGATTTAGATA
TTGATGTTGTACTGCCTATTCCTGAAACATCATGTGATATCGCATTAGAAATCGCTCAGATTATCAATAAGCCTTACCGC
CAAGGCTTTGTTAAGAACCGTTATGTCGGCCGTACTTTTATCATGCCAGGTCAACAAGAGCGTAAGAAATCAGTTCGTCG
TAAATTGAATGCTATTCGTTCAGAATTTAAAGATAAAAACGTTTTATTAGTTGATGACTCCATTGTGCGTGGAACAACCT
CAGAGCAGATTATTGAAATGGCTCGTGATTCTGGTGCTAAAAACGTTTATATGGTGTCAGCTGCTCCTGAGATTCGTTTC
CCTAACGTATATGGTATCGATATGCCAAGTGCAAATGAGCTGATTGCACATGGTCGTGAAGTTGATGAGATTTGCAACGA
GATTGGTGCGGATGCTCTTATTTTCCAAGACCTTTCAGACTTAGTTGATGCGGTTGGTTGCGGTAACAGAGAAATTGAGA
AATTTGAAACCTCTGTTTTCAATGCACATTATGTTACTGGTGATGTTGACCAGAATTACCTAGAGTACTTAGAAAGCCTA
CGTAATGATGATGCAAAACAAGACAACGAAGCACAAAACGTAGCAAGTCTAGATATTTATAACGAAGAGATTTAA

Upstream 100 bases:

>100_bases
GTAGTAATTGAATGGTTCTTCGATTACTTAAAGGATACATCAAGTTTTCTTCCTGGCGCTAAATAGCGCCAGTTTTTTAG
CAAATTGAGGACTCTAGGAC

Downstream 100 bases:

>100_bases
TTTCTTGCTTAATAAAAAAGCCTGTGATTTCACAGGCTTTTTTGTAGAGAGTAAATTAATCAGGTATGTGGTTACTTACA
AAATCGATAAATAAACGTAC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MCGIVGIVGATPVNQSIYDALTVLQHRGQDAAGIITIESNRFRLRKANGLVKDVFETKHMQRLQGNVGIGHVRYPTAGSS
SASEAQPFYVNSPFGITLAHNGNLTNANEIRETLFEQERRHVNTTSDSEVLLNIFAHHLDESKNYPLTNDDIFKTVAKVH
EQIRGAYAVTAMIIGHGMVAFRDPNGIRPLCLGKREIEGRTEYMVASESVALDAVGFDFVRDVAPGEAIYAPFEGGLYTQ
QCAHGPKLNPCIFEFVYFARPDSFIDKISVYSARVEMGKRLGEKIKREWDDLDIDVVLPIPETSCDIALEIAQIINKPYR
QGFVKNRYVGRTFIMPGQQERKKSVRRKLNAIRSEFKDKNVLLVDDSIVRGTTSEQIIEMARDSGAKNVYMVSAAPEIRF
PNVYGIDMPSANELIAHGREVDEICNEIGADALIFQDLSDLVDAVGCGNREIEKFETSVFNAHYVTGDVDQNYLEYLESL
RNDDAKQDNEAQNVASLDIYNEEI

Sequences:

>Translated_504_residues
MCGIVGIVGATPVNQSIYDALTVLQHRGQDAAGIITIESNRFRLRKANGLVKDVFETKHMQRLQGNVGIGHVRYPTAGSS
SASEAQPFYVNSPFGITLAHNGNLTNANEIRETLFEQERRHVNTTSDSEVLLNIFAHHLDESKNYPLTNDDIFKTVAKVH
EQIRGAYAVTAMIIGHGMVAFRDPNGIRPLCLGKREIEGRTEYMVASESVALDAVGFDFVRDVAPGEAIYAPFEGGLYTQ
QCAHGPKLNPCIFEFVYFARPDSFIDKISVYSARVEMGKRLGEKIKREWDDLDIDVVLPIPETSCDIALEIAQIINKPYR
QGFVKNRYVGRTFIMPGQQERKKSVRRKLNAIRSEFKDKNVLLVDDSIVRGTTSEQIIEMARDSGAKNVYMVSAAPEIRF
PNVYGIDMPSANELIAHGREVDEICNEIGADALIFQDLSDLVDAVGCGNREIEKFETSVFNAHYVTGDVDQNYLEYLESL
RNDDAKQDNEAQNVASLDIYNEEI
>Mature_504_residues
MCGIVGIVGATPVNQSIYDALTVLQHRGQDAAGIITIESNRFRLRKANGLVKDVFETKHMQRLQGNVGIGHVRYPTAGSS
SASEAQPFYVNSPFGITLAHNGNLTNANEIRETLFEQERRHVNTTSDSEVLLNIFAHHLDESKNYPLTNDDIFKTVAKVH
EQIRGAYAVTAMIIGHGMVAFRDPNGIRPLCLGKREIEGRTEYMVASESVALDAVGFDFVRDVAPGEAIYAPFEGGLYTQ
QCAHGPKLNPCIFEFVYFARPDSFIDKISVYSARVEMGKRLGEKIKREWDDLDIDVVLPIPETSCDIALEIAQIINKPYR
QGFVKNRYVGRTFIMPGQQERKKSVRRKLNAIRSEFKDKNVLLVDDSIVRGTTSEQIIEMARDSGAKNVYMVSAAPEIRF
PNVYGIDMPSANELIAHGREVDEICNEIGADALIFQDLSDLVDAVGCGNREIEKFETSVFNAHYVTGDVDQNYLEYLESL
RNDDAKQDNEAQNVASLDIYNEEI

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=474, Percent_Identity=36.0759493670886, Blast_Score=267, Evalue=2e-71,
Organism=Escherichia coli, GI1788651, Length=504, Percent_Identity=77.7777777777778, Blast_Score=809, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=223, Percent_Identity=29.5964125560538, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=460, Percent_Identity=34.5652173913044, Blast_Score=239, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6323958, Length=516, Percent_Identity=49.6124031007752, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24659598, Length=483, Percent_Identity=33.9544513457557, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24659604, Length=476, Percent_Identity=34.0336134453782, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI28573187, Length=481, Percent_Identity=33.6798336798337, Blast_Score=239, Evalue=4e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56296; Mature: 56296

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVGATPVNQSIYDALTVLQHRGQDAAGIITIESNRFRLRKANGLVKDVFETKHM
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEHHCCHHHHHHHHHHH
QRLQGNVGIGHVRYPTAGSSSASEAQPFYVNSPFGITLAHNGNLTNANEIRETLFEQERR
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HVNTTSDSEVLLNIFAHHLDESKNYPLTNDDIFKTVAKVHEQIRGAYAVTAMIIGHGMVA
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
FRDPNGIRPLCLGKREIEGRTEYMVASESVALDAVGFDFVRDVAPGEAIYAPFEGGLYTQ
EECCCCCCCCCCCCHHCCCCCCEEEECCCEEEEHHHHHHHHHCCCCCEEECCCCCCCCHH
QCAHGPKLNPCIFEFVYFARPDSFIDKISVYSARVEMGKRLGEKIKREWDDLDIDVVLPI
HHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
PETSCDIALEIAQIINKPYRQGFVKNRYVGRTFIMPGQQERKKSVRRKLNAIRSEFKDKN
CCCCHHHHHHHHHHHCCHHHCCHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCC
VLLVDDSIVRGTTSEQIIEMARDSGAKNVYMVSAAPEIRFPNVYGIDMPSANELIAHGRE
EEEECCCHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCCCCHHHHHHCCCH
VDEICNEIGADALIFQDLSDLVDAVGCGNREIEKFETSVFNAHYVTGDVDQNYLEYLESL
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEEECCCCHHHHHHHHHH
RNDDAKQDNEAQNVASLDIYNEEI
CCCCCCCCCHHHCEEEEEECCCCC
>Mature Secondary Structure
MCGIVGIVGATPVNQSIYDALTVLQHRGQDAAGIITIESNRFRLRKANGLVKDVFETKHM
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEHHCCHHHHHHHHHHH
QRLQGNVGIGHVRYPTAGSSSASEAQPFYVNSPFGITLAHNGNLTNANEIRETLFEQERR
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HVNTTSDSEVLLNIFAHHLDESKNYPLTNDDIFKTVAKVHEQIRGAYAVTAMIIGHGMVA
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
FRDPNGIRPLCLGKREIEGRTEYMVASESVALDAVGFDFVRDVAPGEAIYAPFEGGLYTQ
EECCCCCCCCCCCCHHCCCCCCEEEECCCEEEEHHHHHHHHHCCCCCEEECCCCCCCCHH
QCAHGPKLNPCIFEFVYFARPDSFIDKISVYSARVEMGKRLGEKIKREWDDLDIDVVLPI
HHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
PETSCDIALEIAQIINKPYRQGFVKNRYVGRTFIMPGQQERKKSVRRKLNAIRSEFKDKN
CCCCHHHHHHHHHHHCCHHHCCHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCC
VLLVDDSIVRGTTSEQIIEMARDSGAKNVYMVSAAPEIRFPNVYGIDMPSANELIAHGRE
EEEECCCHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCCCCHHHHHHCCCH
VDEICNEIGADALIFQDLSDLVDAVGCGNREIEKFETSVFNAHYVTGDVDQNYLEYLESL
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEEECCCCHHHHHHHHHH
RNDDAKQDNEAQNVASLDIYNEEI
CCCCCCCCCHHHCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3047685; 6443594; 6277938; 9205837; 9278503; 3040734; 7037784; 9333323; 9514258 [H]