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The map label for this gene is polB [H]

Identifier: 59712235

GI number: 59712235

Start: 1828969

End: 1831314

Strand: Reverse

Name: polB [H]

Synonym: VF_1628

Alternate gene names: 59712235

Gene position: 1831314-1828969 (Counterclockwise)

Preceding gene: 59712236

Following gene: 59712234

Centisome position: 63.2

GC content: 35.51

Gene sequence:

>2346_bases
TTGCTTACACGTCAAACTAGAGAAATTAATAATCAAAGTTTAGTCGACCTCTGGGTTAAGTGTGATGATCATATTGCACA
CTTACTTATTGAGAATGAAGATGCAGTTACTTTCATATTAGAGGATAAGCGCTCTCAGTATGAAGCTTGCTTACAGAAAG
AAAATATCCCTTTTCATATAAAACCGACGGCATTATCCACATTTAATCATGATTCCGTGCTCGGTTTGTATTTTAAAAAT
ACCTACCAAAAACGAGCAGCGACTCAATTTATTGAACAATTACATTTACCTATATATGAAAGCGATATTCGCTTAGCTGA
TCGTTATTTAATGGAGCGTTTTATTTGTGGTGGCCTATCATTTATTGGCAACCCAATACAAAAAAAAGACCATATTGAAT
ATCGTAATGTAAAAATCAAAACGACGGATCGTTCACCTAAATTTAGTATGGTTTCATTAGATATTGAGTGTTCAGAAAAA
GGCATTCTATATTCCGTCGCTCTACATAGTGAACTCGATACACGTATTATCATGGTGGGCGACTCACAGGACAGTAATTT
GTCTATTCAATGGGTGGAAAATGAAAAAGCACTGCTCGTTGCTTTAGAGTCTTGGTTTTATCAATTTGATCCAGACATTA
TTATTGGCTGGAATGTGATTAATTTTGATTTTCGATTATTAATTCAGAGAGCAAAATGGCACAAATTATCTTTTCGTATT
GGTCGGGGTAACAGTTCGCTTTATTGGCGTGATTCCGATAAAAATCGGCAACAAGGTTTTTTATCTTTTCCTGGGCGCGT
TGTGCTAGACGGTATTGATGCTCTGAAAACTGCGACTTATCATTTCTCTAGTTGGTCACTTGAATCAGTAGCTCAAGAGT
TATTGAAAGAAGGTAAATCGATTAATGATCAAAGCGATAAAATGTCTGAGATCAATCGAATGTATCGAGAAGATAAACTT
TCTTTAGCTAAATACAACCTTCAAGATTGTATTTTGGTTACTCGTATATTTGAACATACTCATCTCTTAGAGTTTTTGAT
TGAGCGAACGAAATTAACGGGGGTTGAGCTTGATCGTGTTGGAGGTTCAGTTGCTGCATTTACTAACTTGTATTTACCAA
GGCTTCACAGAGCAGGGTATATTGCACCTAATTTAGAGAGTGAGAATTGGGTTGCAAGTCCTGGTGGCTATGTAATGAAC
TCTAAGCCAGGTTTATATCAATCTGTTATCGTTCTTGATTTTAAATCTCTTTATCCCTCTATTATTCGTACGTTTAATAT
TGATCCTTTAGGCTTGATTGAAGGATTAAAACAAGAAGTAGGTACAGAAGATAATCAAGCCATTGATGGTTTTAGAGGGG
GACGATTTCATCGTTCTAAACACTTCTTACCTAAATTAATTGAAAATTTATGGTCTGCTCGAGATGAAGCAAAAAGAAAT
AATGAAAAGGCATTTTCTCAAGCAATAAAAATCATAATGAACTCATTTTATGGTGTGCTAGGCTCGTCAGGTTGTCGTTT
TTTTGATCATCGATTAGCCTCTTCAATAACCATGCGTGGGCATGAAATTATGAAAATGACTCGTGAGTTGATTGAAGCAG
AAGGTTATGAAGTTATATACGGCGATACTGACTCTACGTTTGTTACTCTTGGGCAATCAATGTCATCGAAAGAAGCTGAC
ACTATTGGTTCATCTTTGGTTGAAAAGATAAATCAGTGGTGGATTTCCCACTTATCTGAAAAATACGCAATTGATTCAGC
ATTAGAAATTGAATATGAAACTCATTATCGAACGTTTTTAATGCCGACGATTCGAGGGTCAGAAACTGGGTCTAAAAAGC
GTTATGCTGGTTTAGTAAAAAAAGAAGGTAAGGAAGAAATTGTATTTAAAGGATTAGAGACAGTTAGAACGGATTGGACT
CCATTATCACAAGAATTTCAGAAAACATTATTCCATAAAGTATTTCATCAACAAGATGTAAAAGAATACGTTCGACAATA
TGTTGATGATACTAAGCAGGGCAAGTTAGATAAGCAGCTCATTTATAGAAAGCGTTTGAGAAGACAGTTGGATGAATATC
AAAAGAACGTTCCTCCTCACGTTAAAGCGGCACGATTAGCAGATTATCGGAATCAACAAATAGGAAAAAGGCCTCAATAT
CAATATGGTGGATGGATAGAGTATGTCATTACCACTGCAGGACCTGAACCGGTTGAATATATAAATAATCCGATTGATTA
TGACCATTATGTAGAGAAACAATTGAAACCGATCGCGGATGGAATTCTCCCTTTTATCGGTTTAGATTTTAATGAATTAT
CTAACTCTCAATTAGGTCTATTTTAA

Upstream 100 bases:

>100_bases
CTTGCTTTACTTAATGAATTTGGAGATTGGGTTGTGGCTGAATTTAATAAAATTGAACGAAAAATAGGTTAATAACTTGA
ATTCACCTCAACAGGGTTTT

Downstream 100 bases:

>100_bases
GAATTATTTTTATTGTATATTGAAGAAACCGGATCAAAGTCAATAGCTTTTTAATAGCAAAACTTTGCGGCCTAACGCAT
AATCAATATGCTTTTATCTG

Product: DNA polymerase II

Products: NA

Alternate protein names: Pol II [H]

Number of amino acids: Translated: 781; Mature: 781

Protein sequence:

>781_residues
MLTRQTREINNQSLVDLWVKCDDHIAHLLIENEDAVTFILEDKRSQYEACLQKENIPFHIKPTALSTFNHDSVLGLYFKN
TYQKRAATQFIEQLHLPIYESDIRLADRYLMERFICGGLSFIGNPIQKKDHIEYRNVKIKTTDRSPKFSMVSLDIECSEK
GILYSVALHSELDTRIIMVGDSQDSNLSIQWVENEKALLVALESWFYQFDPDIIIGWNVINFDFRLLIQRAKWHKLSFRI
GRGNSSLYWRDSDKNRQQGFLSFPGRVVLDGIDALKTATYHFSSWSLESVAQELLKEGKSINDQSDKMSEINRMYREDKL
SLAKYNLQDCILVTRIFEHTHLLEFLIERTKLTGVELDRVGGSVAAFTNLYLPRLHRAGYIAPNLESENWVASPGGYVMN
SKPGLYQSVIVLDFKSLYPSIIRTFNIDPLGLIEGLKQEVGTEDNQAIDGFRGGRFHRSKHFLPKLIENLWSARDEAKRN
NEKAFSQAIKIIMNSFYGVLGSSGCRFFDHRLASSITMRGHEIMKMTRELIEAEGYEVIYGDTDSTFVTLGQSMSSKEAD
TIGSSLVEKINQWWISHLSEKYAIDSALEIEYETHYRTFLMPTIRGSETGSKKRYAGLVKKEGKEEIVFKGLETVRTDWT
PLSQEFQKTLFHKVFHQQDVKEYVRQYVDDTKQGKLDKQLIYRKRLRRQLDEYQKNVPPHVKAARLADYRNQQIGKRPQY
QYGGWIEYVITTAGPEPVEYINNPIDYDHYVEKQLKPIADGILPFIGLDFNELSNSQLGLF

Sequences:

>Translated_781_residues
MLTRQTREINNQSLVDLWVKCDDHIAHLLIENEDAVTFILEDKRSQYEACLQKENIPFHIKPTALSTFNHDSVLGLYFKN
TYQKRAATQFIEQLHLPIYESDIRLADRYLMERFICGGLSFIGNPIQKKDHIEYRNVKIKTTDRSPKFSMVSLDIECSEK
GILYSVALHSELDTRIIMVGDSQDSNLSIQWVENEKALLVALESWFYQFDPDIIIGWNVINFDFRLLIQRAKWHKLSFRI
GRGNSSLYWRDSDKNRQQGFLSFPGRVVLDGIDALKTATYHFSSWSLESVAQELLKEGKSINDQSDKMSEINRMYREDKL
SLAKYNLQDCILVTRIFEHTHLLEFLIERTKLTGVELDRVGGSVAAFTNLYLPRLHRAGYIAPNLESENWVASPGGYVMN
SKPGLYQSVIVLDFKSLYPSIIRTFNIDPLGLIEGLKQEVGTEDNQAIDGFRGGRFHRSKHFLPKLIENLWSARDEAKRN
NEKAFSQAIKIIMNSFYGVLGSSGCRFFDHRLASSITMRGHEIMKMTRELIEAEGYEVIYGDTDSTFVTLGQSMSSKEAD
TIGSSLVEKINQWWISHLSEKYAIDSALEIEYETHYRTFLMPTIRGSETGSKKRYAGLVKKEGKEEIVFKGLETVRTDWT
PLSQEFQKTLFHKVFHQQDVKEYVRQYVDDTKQGKLDKQLIYRKRLRRQLDEYQKNVPPHVKAARLADYRNQQIGKRPQY
QYGGWIEYVITTAGPEPVEYINNPIDYDHYVEKQLKPIADGILPFIGLDFNELSNSQLGLF
>Mature_781_residues
MLTRQTREINNQSLVDLWVKCDDHIAHLLIENEDAVTFILEDKRSQYEACLQKENIPFHIKPTALSTFNHDSVLGLYFKN
TYQKRAATQFIEQLHLPIYESDIRLADRYLMERFICGGLSFIGNPIQKKDHIEYRNVKIKTTDRSPKFSMVSLDIECSEK
GILYSVALHSELDTRIIMVGDSQDSNLSIQWVENEKALLVALESWFYQFDPDIIIGWNVINFDFRLLIQRAKWHKLSFRI
GRGNSSLYWRDSDKNRQQGFLSFPGRVVLDGIDALKTATYHFSSWSLESVAQELLKEGKSINDQSDKMSEINRMYREDKL
SLAKYNLQDCILVTRIFEHTHLLEFLIERTKLTGVELDRVGGSVAAFTNLYLPRLHRAGYIAPNLESENWVASPGGYVMN
SKPGLYQSVIVLDFKSLYPSIIRTFNIDPLGLIEGLKQEVGTEDNQAIDGFRGGRFHRSKHFLPKLIENLWSARDEAKRN
NEKAFSQAIKIIMNSFYGVLGSSGCRFFDHRLASSITMRGHEIMKMTRELIEAEGYEVIYGDTDSTFVTLGQSMSSKEAD
TIGSSLVEKINQWWISHLSEKYAIDSALEIEYETHYRTFLMPTIRGSETGSKKRYAGLVKKEGKEEIVFKGLETVRTDWT
PLSQEFQKTLFHKVFHQQDVKEYVRQYVDDTKQGKLDKQLIYRKRLRRQLDEYQKNVPPHVKAARLADYRNQQIGKRPQY
QYGGWIEYVITTAGPEPVEYINNPIDYDHYVEKQLKPIADGILPFIGLDFNELSNSQLGLF

Specific function: Thought to be involved in DNA repair and/or mutagenesis [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=681, Percent_Identity=27.9001468428781, Blast_Score=180, Evalue=5e-45,
Organism=Homo sapiens, GI106507301, Length=664, Percent_Identity=24.8493975903614, Blast_Score=136, Evalue=1e-31,
Organism=Escherichia coli, GI1786246, Length=787, Percent_Identity=50.0635324015248, Blast_Score=783, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17559488, Length=637, Percent_Identity=27.6295133437991, Blast_Score=169, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI32565317, Length=632, Percent_Identity=25.3164556962025, Blast_Score=132, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6320101, Length=630, Percent_Identity=26.031746031746, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324227, Length=616, Percent_Identity=26.6233766233766, Blast_Score=128, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6325090, Length=750, Percent_Identity=22.9333333333333, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24664937, Length=698, Percent_Identity=26.9340974212034, Blast_Score=168, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24648780, Length=604, Percent_Identity=23.3443708609272, Blast_Score=120, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 90403; Mature: 90403

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRQTREINNQSLVDLWVKCDDHIAHLLIENEDAVTFILEDKRSQYEACLQKENIPFHI
CCCCCCHHCCCCCEEHHEECCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHCCCCCEEE
KPTALSTFNHDSVLGLYFKNTYQKRAATQFIEQLHLPIYESDIRLADRYLMERFICGGLS
ECCEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHH
FIGNPIQKKDHIEYRNVKIKTTDRSPKFSMVSLDIECSEKGILYSVALHSELDTRIIMVG
HHCCCCCCCCCCEEEEEEEEECCCCCCEEEEEEEEEECCCCEEEEEEECCCCCCEEEEEE
DSQDSNLSIQWVENEKALLVALESWFYQFDPDIIIGWNVINFDFRLLIQRAKWHKLSFRI
CCCCCCEEEEEECCCCEEEEEEHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHEEEEEEE
GRGNSSLYWRDSDKNRQQGFLSFPGRVVLDGIDALKTATYHFSSWSLESVAQELLKEGKS
ECCCCEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHEEECCCCHHHHHHHHHHCCCC
INDQSDKMSEINRMYREDKLSLAKYNLQDCILVTRIFEHTHLLEFLIERTKLTGVELDRV
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHC
GGSVAAFTNLYLPRLHRAGYIAPNLESENWVASPGGYVMNSKPGLYQSVIVLDFKSLYPS
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEECCCCCHHHHHHHHHHHHHHHH
IIRTFNIDPLGLIEGLKQEVGTEDNQAIDGFRGGRFHRSKHFLPKLIENLWSARDEAKRN
HHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
NEKAFSQAIKIIMNSFYGVLGSSGCRFFDHRLASSITMRGHEIMKMTRELIEAEGYEVIY
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEE
GDTDSTFVTLGQSMSSKEADTIGSSLVEKINQWWISHLSEKYAIDSALEIEYETHYRTFL
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEE
MPTIRGSETGSKKRYAGLVKKEGKEEIVFKGLETVRTDWTPLSQEFQKTLFHKVFHQQDV
EEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KEYVRQYVDDTKQGKLDKQLIYRKRLRRQLDEYQKNVPPHVKAARLADYRNQQIGKRPQY
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCC
QYGGWIEYVITTAGPEPVEYINNPIDYDHYVEKQLKPIADGILPFIGLDFNELSNSQLGL
CCCCEEEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCC
F
C
>Mature Secondary Structure
MLTRQTREINNQSLVDLWVKCDDHIAHLLIENEDAVTFILEDKRSQYEACLQKENIPFHI
CCCCCCHHCCCCCEEHHEECCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHCCCCCEEE
KPTALSTFNHDSVLGLYFKNTYQKRAATQFIEQLHLPIYESDIRLADRYLMERFICGGLS
ECCEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHH
FIGNPIQKKDHIEYRNVKIKTTDRSPKFSMVSLDIECSEKGILYSVALHSELDTRIIMVG
HHCCCCCCCCCCEEEEEEEEECCCCCCEEEEEEEEEECCCCEEEEEEECCCCCCEEEEEE
DSQDSNLSIQWVENEKALLVALESWFYQFDPDIIIGWNVINFDFRLLIQRAKWHKLSFRI
CCCCCCEEEEEECCCCEEEEEEHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHEEEEEEE
GRGNSSLYWRDSDKNRQQGFLSFPGRVVLDGIDALKTATYHFSSWSLESVAQELLKEGKS
ECCCCEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHEEECCCCHHHHHHHHHHCCCC
INDQSDKMSEINRMYREDKLSLAKYNLQDCILVTRIFEHTHLLEFLIERTKLTGVELDRV
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHC
GGSVAAFTNLYLPRLHRAGYIAPNLESENWVASPGGYVMNSKPGLYQSVIVLDFKSLYPS
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEECCCCCHHHHHHHHHHHHHHHH
IIRTFNIDPLGLIEGLKQEVGTEDNQAIDGFRGGRFHRSKHFLPKLIENLWSARDEAKRN
HHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
NEKAFSQAIKIIMNSFYGVLGSSGCRFFDHRLASSITMRGHEIMKMTRELIEAEGYEVIY
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEE
GDTDSTFVTLGQSMSSKEADTIGSSLVEKINQWWISHLSEKYAIDSALEIEYETHYRTFL
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEE
MPTIRGSETGSKKRYAGLVKKEGKEEIVFKGLETVRTDWTPLSQEFQKTLFHKVFHQQDV
EEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KEYVRQYVDDTKQGKLDKQLIYRKRLRRQLDEYQKNVPPHVKAARLADYRNQQIGKRPQY
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCC
QYGGWIEYVITTAGPEPVEYINNPIDYDHYVEKQLKPIADGILPFIGLDFNELSNSQLGL
CCCCEEEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034216; 2261080; 1630901; 9278503; 2217198; 9079692 [H]