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The map label for this gene is gacA [H]

Identifier: 59712234

GI number: 59712234

Start: 1828148

End: 1828792

Strand: Reverse

Name: gacA [H]

Synonym: VF_1627

Alternate gene names: 59712234

Gene position: 1828792-1828148 (Counterclockwise)

Preceding gene: 59712235

Following gene: 59712233

Centisome position: 63.12

GC content: 40.0

Gene sequence:

>645_bases
GTGATTAAAGTATTCCTTGTAGATGATCATGAGCTGGTTCGCACAGGGATTAGACGTATTCTTGAAGACGTCCGTGGAAT
TAAAGTAGTAGGGGAAGCTGACAGCGGTGAAGATGCCGTTAAATGGTGTCGAACAGAGCAGGCAGATATTGTTCTTATGG
ATATGAACATGCCTGGCATTGGTGGCTTAGAAGCAACCAAAAAGATTTTAAGATTTAACCCAGATGTGAAAGTTATAGTT
TTGACTATTCATACTGAAAACCCATTTCCGACTAAAGTTATGCAAGCCGGTGCTGCTGGTTATTTAACTAAGGGTGCAGG
TCCTGACGAAATGGTTAATGCGATTCGTATGGTACAAAGTGGTCAACGCTACTTATCACCAGAAATTGCACAACAGATGG
CGCTAAGTCAATTTACGCCAGATGCTGAGAATCCATTTAAAGAGCTTTCTGAACGAGAACTACAAATAATGATGATGATC
ACTAAAGGCGAAAAAGTGACGGATATTTCCGAACAACTGAATTTAAGCCCTAAAACAGTGAACAGCTATCGTTACCGTTT
ATTCAGTAAGTTAAATATAAGTGGAGACGTTGAATTAACTCATCTAGCTATTCGTCATGGTATTTTAGATGCTGAGACGC
TGTAG

Upstream 100 bases:

>100_bases
GCATAATCAATATGCTTTTATCTGTTTTTTTAAGGTAAAATAGACAGTTAGTTAAGACCTCTACATAGAGGTGGCTGTAG
ATTAAATCGGAGTGTCAGTT

Downstream 100 bases:

>100_bases
TGCCTTCCTTTGATTCAAAAGCGTTCCTAAAATCTGTAACACATCAGCCTGGCGTTTATCGAATGTATAACGCCGAGGCT
GACGTTATTTATGTAGGTAA

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MIKVFLVDDHELVRTGIRRILEDVRGIKVVGEADSGEDAVKWCRTEQADIVLMDMNMPGIGGLEATKKILRFNPDVKVIV
LTIHTENPFPTKVMQAGAAGYLTKGAGPDEMVNAIRMVQSGQRYLSPEIAQQMALSQFTPDAENPFKELSERELQIMMMI
TKGEKVTDISEQLNLSPKTVNSYRYRLFSKLNISGDVELTHLAIRHGILDAETL

Sequences:

>Translated_214_residues
MIKVFLVDDHELVRTGIRRILEDVRGIKVVGEADSGEDAVKWCRTEQADIVLMDMNMPGIGGLEATKKILRFNPDVKVIV
LTIHTENPFPTKVMQAGAAGYLTKGAGPDEMVNAIRMVQSGQRYLSPEIAQQMALSQFTPDAENPFKELSERELQIMMMI
TKGEKVTDISEQLNLSPKTVNSYRYRLFSKLNISGDVELTHLAIRHGILDAETL
>Mature_214_residues
MIKVFLVDDHELVRTGIRRILEDVRGIKVVGEADSGEDAVKWCRTEQADIVLMDMNMPGIGGLEATKKILRFNPDVKVIV
LTIHTENPFPTKVMQAGAAGYLTKGAGPDEMVNAIRMVQSGQRYLSPEIAQQMALSQFTPDAENPFKELSERELQIMMMI
TKGEKVTDISEQLNLSPKTVNSYRYRLFSKLNISGDVELTHLAIRHGILDAETL

Specific function: Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. UvrY activates the transcription of the untranslated csrB RNA and of barA, in an autoregulatory loop. Mediates the

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=215, Percent_Identity=76.7441860465116, Blast_Score=351, Evalue=2e-98,
Organism=Escherichia coli, GI1790102, Length=200, Percent_Identity=34.5, Blast_Score=120, Evalue=5e-29,
Organism=Escherichia coli, GI1788521, Length=205, Percent_Identity=32.1951219512195, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1786747, Length=200, Percent_Identity=26.5, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788712, Length=203, Percent_Identity=27.0935960591133, Blast_Score=90, Evalue=9e-20,
Organism=Escherichia coli, GI1788546, Length=203, Percent_Identity=27.5862068965517, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787473, Length=182, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23915; Mature: 23915

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVFLVDDHELVRTGIRRILEDVRGIKVVGEADSGEDAVKWCRTEQADIVLMDMNMPGI
CEEEEEECCHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHCCCCCCEEEEECCCCCC
GGLEATKKILRFNPDVKVIVLTIHTENPFPTKVMQAGAAGYLTKGAGPDEMVNAIRMVQS
CCHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
GQRYLSPEIAQQMALSQFTPDAENPFKELSERELQIMMMITKGEKVTDISEQLNLSPKTV
CCHHCCHHHHHHHHHHHCCCCCCCHHHHHHHCCEEEEEEEECCCCHHHHHHHHCCCCCHH
NSYRYRLFSKLNISGDVELTHLAIRHGILDAETL
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIKVFLVDDHELVRTGIRRILEDVRGIKVVGEADSGEDAVKWCRTEQADIVLMDMNMPGI
CEEEEEECCHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHCCCCCCEEEEECCCCCC
GGLEATKKILRFNPDVKVIVLTIHTENPFPTKVMQAGAAGYLTKGAGPDEMVNAIRMVQS
CCHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHC
GQRYLSPEIAQQMALSQFTPDAENPFKELSERELQIMMMITKGEKVTDISEQLNLSPKTV
CCHHCCHHHHHHHHHHHCCCCCCCHHHHHHHCCEEEEEEEECCCCHHHHHHHHCCCCCHH
NSYRYRLFSKLNISGDVELTHLAIRHGILDAETL
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]