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The map label for this gene is hlyB [H]

Identifier: 59712225

GI number: 59712225

Start: 1813333

End: 1814973

Strand: Reverse

Name: hlyB [H]

Synonym: VF_1618

Alternate gene names: 59712225

Gene position: 1814973-1813333 (Counterclockwise)

Preceding gene: 59712226

Following gene: 59712221

Centisome position: 62.64

GC content: 37.78

Gene sequence:

>1641_bases
ATGAAGCTAATTGGACGTTTATCAATCAGTTACAAAATAGCAATGCCGATTATTGCTGTTGTTCTATTATTTAGTGTTAT
TTCTGTTTTGAATATTGTGAATTTAAATAAACAGACAGAAATGAATCACACGCTTGTTGAGTTAGTAGAACCTGTTAACG
ATAGCCTGGAAGATGCCTATCGCGATCTTTATCAAGTTATCGCTGCAACTCAAGGTCTTATGTTGTCAAAGTCTCAAGTG
GATATTGAACATCATACTTTTGAATTCAAAGATAATGCGTATAAAGCCGTTCCTAGAATGAAAAAAGTACAAGATTTATA
TGACAACCAGATTCTGTCTATGAATACGGCATCAGAATTGACTGTTTTGGTAACGGCAACTCAAAGATGGATTCAACTTT
ACGAACCGTTATTTGAGCATCCTGAAACTGCTCAAGTTTATTATGCTCAAAATAGTGCAGAGCTAGAGAAACAATTTAAA
GTCATTAGAAAACAACTAAAATCAATTAGTCGTTTAATTATGGCGGAACAAGTTAAGCTAAGAGAACAAAGTCAAACAGG
TATAGAGTTTGGGAAACTGGTTTCTCAAGTTAGTGCGATTAGTGCGATTATTGTTGCTATGTTAGCCCTTTGGCTATCGA
TTAATTGGATCGTAAAACCGATTCGTTCACTTGAAAGTGCAATGTCGGATGTTGCCTCTGGTGAAGGTGATTTATCAAAA
CGAATTAGCATTGATTCTACTGATGAACTAGGAAAGTTAGCCCAAGCGTTCAATCAGTTTATTTCTAAAATTCACGGAAC
AGTACAAGAGGTTATAGCCTCATCTAATGCTGTACGTGCTGAAATGGAAAGTATTAAAGCACAAACTCAAAACGTAGCTC
AATTCTCGATGAATCAGCAACAAGAGAGTGAAGTAGTTGCAGCAGCTGTGCATGAAATGCAAGTAACAAGTTCGACGGTT
AATGAGAATGCGAATGACGCTGCGGAGGCGAGTCAAGGAGCGACTGCGGAAGTGGAGGTTACTGATAATATTCTTCAACA
AACTGTGGGGTCAATTCAGAGTTTAGCGGATGAGATAAATCAGGCTGGCAAAGTGATTCAAACATTAGATACTGATGTTG
CAAATATTGCCTCTATTTTAGATGTGATTAAAGGTATTGCTGAGCAAACCAACTTATTGGCACTTAATGCAGCGATTGAA
GCAGCTCGTGCGGGTGAACAAGGGCGAGGTTTTGCTGTTGTTGCTGATGAAGTAAGAGCGCTAGCAAGTAAGACTCAAGA
TAGCACTGGTGAAATTCAAAAAATGATCGAACGCCTTCAACTAGGTGCAAAAGAAGCGGTACAAGCGATTAATGCGAGTA
ATCAGAGTGGTGTTGAAACAATAGAGTTAGCTAATCAAGCATCACAATCTTTAGCTCAAATTACCAATGCCATTATTGTT
ATGAACGATATGAATACACATATTGCAACGGCTGCTAATCAACAAAGCCAAGTGAGCGAAGATGTTAATAATAACGTTCA
ACGAATTACTGATAACAGTAGACAAATGGTTGAAATGGTAAACCAATCAGAAAGCGCTTGTGCTTTATTATCCGAGCAAT
GTGAGCGATTAGATCAATTGGTGTCACAATTTAGAGTGTAA

Upstream 100 bases:

>100_bases
GAAATTCATTTCATATATTCACGTTATTTATAATTATGTTTGTAATGTTATTTGATAATATCCAAAAAAATATTAGTGCT
GGTTTGCCTTAGGAGAGGTT

Downstream 100 bases:

>100_bases
TATTAACTGTAAGAATGAAAGGCTTATCATTGTTGATAAGCCTTTTTTTTATAAATGTGTTATCACAATAACTTTACCTG
TTGTTGAGTTATACGAAATA

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MKLIGRLSISYKIAMPIIAVVLLFSVISVLNIVNLNKQTEMNHTLVELVEPVNDSLEDAYRDLYQVIAATQGLMLSKSQV
DIEHHTFEFKDNAYKAVPRMKKVQDLYDNQILSMNTASELTVLVTATQRWIQLYEPLFEHPETAQVYYAQNSAELEKQFK
VIRKQLKSISRLIMAEQVKLREQSQTGIEFGKLVSQVSAISAIIVAMLALWLSINWIVKPIRSLESAMSDVASGEGDLSK
RISIDSTDELGKLAQAFNQFISKIHGTVQEVIASSNAVRAEMESIKAQTQNVAQFSMNQQQESEVVAAAVHEMQVTSSTV
NENANDAAEASQGATAEVEVTDNILQQTVGSIQSLADEINQAGKVIQTLDTDVANIASILDVIKGIAEQTNLLALNAAIE
AARAGEQGRGFAVVADEVRALASKTQDSTGEIQKMIERLQLGAKEAVQAINASNQSGVETIELANQASQSLAQITNAIIV
MNDMNTHIATAANQQSQVSEDVNNNVQRITDNSRQMVEMVNQSESACALLSEQCERLDQLVSQFRV

Sequences:

>Translated_546_residues
MKLIGRLSISYKIAMPIIAVVLLFSVISVLNIVNLNKQTEMNHTLVELVEPVNDSLEDAYRDLYQVIAATQGLMLSKSQV
DIEHHTFEFKDNAYKAVPRMKKVQDLYDNQILSMNTASELTVLVTATQRWIQLYEPLFEHPETAQVYYAQNSAELEKQFK
VIRKQLKSISRLIMAEQVKLREQSQTGIEFGKLVSQVSAISAIIVAMLALWLSINWIVKPIRSLESAMSDVASGEGDLSK
RISIDSTDELGKLAQAFNQFISKIHGTVQEVIASSNAVRAEMESIKAQTQNVAQFSMNQQQESEVVAAAVHEMQVTSSTV
NENANDAAEASQGATAEVEVTDNILQQTVGSIQSLADEINQAGKVIQTLDTDVANIASILDVIKGIAEQTNLLALNAAIE
AARAGEQGRGFAVVADEVRALASKTQDSTGEIQKMIERLQLGAKEAVQAINASNQSGVETIELANQASQSLAQITNAIIV
MNDMNTHIATAANQQSQVSEDVNNNVQRITDNSRQMVEMVNQSESACALLSEQCERLDQLVSQFRV
>Mature_546_residues
MKLIGRLSISYKIAMPIIAVVLLFSVISVLNIVNLNKQTEMNHTLVELVEPVNDSLEDAYRDLYQVIAATQGLMLSKSQV
DIEHHTFEFKDNAYKAVPRMKKVQDLYDNQILSMNTASELTVLVTATQRWIQLYEPLFEHPETAQVYYAQNSAELEKQFK
VIRKQLKSISRLIMAEQVKLREQSQTGIEFGKLVSQVSAISAIIVAMLALWLSINWIVKPIRSLESAMSDVASGEGDLSK
RISIDSTDELGKLAQAFNQFISKIHGTVQEVIASSNAVRAEMESIKAQTQNVAQFSMNQQQESEVVAAAVHEMQVTSSTV
NENANDAAEASQGATAEVEVTDNILQQTVGSIQSLADEINQAGKVIQTLDTDVANIASILDVIKGIAEQTNLLALNAAIE
AARAGEQGRGFAVVADEVRALASKTQDSTGEIQKMIERLQLGAKEAVQAINASNQSGVETIELANQASQSLAQITNAIIV
MNDMNTHIATAANQQSQVSEDVNNNVQRITDNSRQMVEMVNQSESACALLSEQCERLDQLVSQFRV

Specific function: May form a pore through which the hemolysin can be exported [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1788195, Length=345, Percent_Identity=33.0434782608696, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI2367378, Length=358, Percent_Identity=31.0055865921788, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788194, Length=360, Percent_Identity=30.2777777777778, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1787690, Length=350, Percent_Identity=29.4285714285714, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1789453, Length=435, Percent_Identity=25.2873563218391, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660
- InterPro:   IPR000727 [H]

Pfam domain/function: PF00672 HAMP; PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 59839; Mature: 59839

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2 ; PS50192 T_SNARE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIGRLSISYKIAMPIIAVVLLFSVISVLNIVNLNKQTEMNHTLVELVEPVNDSLEDAY
CCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHH
RDLYQVIAATQGLMLSKSQVDIEHHTFEFKDNAYKAVPRMKKVQDLYDNQILSMNTASEL
HHHHHHHHHHCCHHCCCCCCCHHHCEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCE
TVLVTATQRWIQLYEPLFEHPETAQVYYAQNSAELEKQFKVIRKQLKSISRLIMAEQVKL
EEEHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REQSQTGIEFGKLVSQVSAISAIIVAMLALWLSINWIVKPIRSLESAMSDVASGEGDLSK
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
RISIDSTDELGKLAQAFNQFISKIHGTVQEVIASSNAVRAEMESIKAQTQNVAQFSMNQQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHH
QESEVVAAAVHEMQVTSSTVNENANDAAEASQGATAEVEVTDNILQQTVGSIQSLADEIN
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
QAGKVIQTLDTDVANIASILDVIKGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHH
LASKTQDSTGEIQKMIERLQLGAKEAVQAINASNQSGVETIELANQASQSLAQITNAIIV
HHHHCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEE
MNDMNTHIATAANQQSQVSEDVNNNVQRITDNSRQMVEMVNQSESACALLSEQCERLDQL
EECCCCHHHHHCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VSQFRV
HHHHCC
>Mature Secondary Structure
MKLIGRLSISYKIAMPIIAVVLLFSVISVLNIVNLNKQTEMNHTLVELVEPVNDSLEDAY
CCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHH
RDLYQVIAATQGLMLSKSQVDIEHHTFEFKDNAYKAVPRMKKVQDLYDNQILSMNTASEL
HHHHHHHHHHCCHHCCCCCCCHHHCEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCE
TVLVTATQRWIQLYEPLFEHPETAQVYYAQNSAELEKQFKVIRKQLKSISRLIMAEQVKL
EEEHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REQSQTGIEFGKLVSQVSAISAIIVAMLALWLSINWIVKPIRSLESAMSDVASGEGDLSK
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
RISIDSTDELGKLAQAFNQFISKIHGTVQEVIASSNAVRAEMESIKAQTQNVAQFSMNQQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHH
QESEVVAAAVHEMQVTSSTVNENANDAAEASQGATAEVEVTDNILQQTVGSIQSLADEIN
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
QAGKVIQTLDTDVANIASILDVIKGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHH
LASKTQDSTGEIQKMIERLQLGAKEAVQAINASNQSGVETIELANQASQSLAQITNAIIV
HHHHCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEE
MNDMNTHIATAANQQSQVSEDVNNNVQRITDNSRQMVEMVNQSESACALLSEQCERLDQL
EECCCCHHHHHCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VSQFRV
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2162464; 10952301 [H]