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The map label for this gene is cls [H]

Identifier: 59712213

GI number: 59712213

Start: 1802048

End: 1803502

Strand: Direct

Name: cls [H]

Synonym: VF_1606

Alternate gene names: 59712213

Gene position: 1802048-1803502 (Clockwise)

Preceding gene: 59712211

Following gene: 59712215

Centisome position: 62.19

GC content: 40.62

Gene sequence:

>1455_bases
ATGGATAAATTTTATCAAGCACTTACAATTGTTAGCTTCGTTTTATATTGGCTTCTCGTAGCAGGCGTAACAATTCGTGT
TGTTTTCAAACCAAGACCCGTAGGCGTATCATTAGCTTGGCTTATGATTATTTACATTCTTCCTGTTATTGGGGTCATGG
CTTATTTTTTAATTGGTGAGCTTAACTTAGGTCGAACTCGTGGTGAACGTGCACGAGCTATGTTTACGCCTTATGAACAG
TGGTTTGCACAAATTCATCATTGCCAATATCACCAACCACATTCGATTTCCCATCGTATCTCAGCGATTCACAATTTATG
CTCTAATCGCCTCGGGATCCCTGCATTAAGTGGCGATACACTCTCGCTATTAAATACCCCTTCAGAAATTCTCAATGCGA
TTATTTCTGATATAGAAAAAGCCGAACATGAAATAAATATGGAGTTTTATATTTGGCATCACGGTGGGCTGGCTGACAGT
GTTGCAAGCGCACTAATTGTAGCTGCAAAACGAGGCGTAACTGTCAATGTGCTGCTTGACTCCGCAGGTAGTATGGATTT
TTTTAAGAGTCACTGGCCTAAATTACTCAAGCAATCCGGTGTCAACATTGTAGAAGCTCTAAGTGTTAGTCCATTTCGTA
TGTTCTTTCGACGCCTAGATCTACGCTTGCATCGAAAAATTGTTGTGATTGATAACCGTATCGCTTATACCGGCTCAATG
AACCTAGTTGATCCACAGTACTTCAAACAAAACTCAGGTGTTGGCCAATGGATTGACGTAATGGTTCGCCTTACAGGACC
TAATGTCGCTATTTTAAATACTATTCATGCGTGGGATTGGGAAGTTGAAACGGGTATTCGCGAGCTACCTCATTTACCGG
AGTGCCCTGCCTCTATTGACCCATTTAATCATACAACTCAAGTGGTTCCTTCTGGCCCAGGTATGCCAGATGACATTATT
CAGCAAGTGCTTATTACTGCTATTTGCCAAGCAAGAAAATCCATTACCATCACCACGCCGTATTTTGTGCCTAGTGAGAT
TTTATTGCACACCTTAAGAACCACTGCACACCGCGGTGTTGAAGTGAAACTCATACTGCCTAAGAAAAATGACTCATTAA
TGGTGAACTGGGCATCAAAATCTTTTTTTGAAACCCTCTTAAAAGCCGGAGTTCAAATACATGAATTTAATGGTGGTTTA
TTACATACAAAATCGGTCGTTATTGATGAAAGCCATTGTATTATTGGAACCGTAAACTTAGATATGCGCAGCTTCTGGCT
CAACTTTGAAGTAAGTTTGATCGTCGATGATCCTGAGTTTACAAAACAATTATCATGGGTTTTAGATGGCTACCTAGAGC
AATCAACTCAAGTCTGTTTTGATACATGGCGTCATAGAAGTTGGGGAAATCGCTTTACCGAACGATTCTTCTCTATGTTC
AGCCCATTACTCTAA

Upstream 100 bases:

>100_bases
ATAATTTTTATTTTGTAAATTTTTGCATTCCTATCTACTCTTTTCTTATCACTCCTGATTTAATAGAGATCCTCTTACTT
TTTTCACTGACAGAGCGCTA

Downstream 100 bases:

>100_bases
AAACACATAAGAAAAAGCCAGTGACCTCTTTGCCAAGGTCACTGGCTTTTTTAATGTTTACTTTATTGTGATATTTAACA
AGATGGCTGTATTTAACGAA

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MDKFYQALTIVSFVLYWLLVAGVTIRVVFKPRPVGVSLAWLMIIYILPVIGVMAYFLIGELNLGRTRGERARAMFTPYEQ
WFAQIHHCQYHQPHSISHRISAIHNLCSNRLGIPALSGDTLSLLNTPSEILNAIISDIEKAEHEINMEFYIWHHGGLADS
VASALIVAAKRGVTVNVLLDSAGSMDFFKSHWPKLLKQSGVNIVEALSVSPFRMFFRRLDLRLHRKIVVIDNRIAYTGSM
NLVDPQYFKQNSGVGQWIDVMVRLTGPNVAILNTIHAWDWEVETGIRELPHLPECPASIDPFNHTTQVVPSGPGMPDDII
QQVLITAICQARKSITITTPYFVPSEILLHTLRTTAHRGVEVKLILPKKNDSLMVNWASKSFFETLLKAGVQIHEFNGGL
LHTKSVVIDESHCIIGTVNLDMRSFWLNFEVSLIVDDPEFTKQLSWVLDGYLEQSTQVCFDTWRHRSWGNRFTERFFSMF
SPLL

Sequences:

>Translated_484_residues
MDKFYQALTIVSFVLYWLLVAGVTIRVVFKPRPVGVSLAWLMIIYILPVIGVMAYFLIGELNLGRTRGERARAMFTPYEQ
WFAQIHHCQYHQPHSISHRISAIHNLCSNRLGIPALSGDTLSLLNTPSEILNAIISDIEKAEHEINMEFYIWHHGGLADS
VASALIVAAKRGVTVNVLLDSAGSMDFFKSHWPKLLKQSGVNIVEALSVSPFRMFFRRLDLRLHRKIVVIDNRIAYTGSM
NLVDPQYFKQNSGVGQWIDVMVRLTGPNVAILNTIHAWDWEVETGIRELPHLPECPASIDPFNHTTQVVPSGPGMPDDII
QQVLITAICQARKSITITTPYFVPSEILLHTLRTTAHRGVEVKLILPKKNDSLMVNWASKSFFETLLKAGVQIHEFNGGL
LHTKSVVIDESHCIIGTVNLDMRSFWLNFEVSLIVDDPEFTKQLSWVLDGYLEQSTQVCFDTWRHRSWGNRFTERFFSMF
SPLL
>Mature_484_residues
MDKFYQALTIVSFVLYWLLVAGVTIRVVFKPRPVGVSLAWLMIIYILPVIGVMAYFLIGELNLGRTRGERARAMFTPYEQ
WFAQIHHCQYHQPHSISHRISAIHNLCSNRLGIPALSGDTLSLLNTPSEILNAIISDIEKAEHEINMEFYIWHHGGLADS
VASALIVAAKRGVTVNVLLDSAGSMDFFKSHWPKLLKQSGVNIVEALSVSPFRMFFRRLDLRLHRKIVVIDNRIAYTGSM
NLVDPQYFKQNSGVGQWIDVMVRLTGPNVAILNTIHAWDWEVETGIRELPHLPECPASIDPFNHTTQVVPSGPGMPDDII
QQVLITAICQARKSITITTPYFVPSEILLHTLRTTAHRGVEVKLILPKKNDSLMVNWASKSFFETLLKAGVQIHEFNGGL
LHTKSVVIDESHCIIGTVNLDMRSFWLNFEVSLIVDDPEFTKQLSWVLDGYLEQSTQVCFDTWRHRSWGNRFTERFFSMF
SPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=488, Percent_Identity=48.9754098360656, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI1787007, Length=358, Percent_Identity=29.8882681564246, Blast_Score=135, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 54962; Mature: 54962

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFYQALTIVSFVLYWLLVAGVTIRVVFKPRPVGVSLAWLMIIYILPVIGVMAYFLIGE
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
LNLGRTRGERARAMFTPYEQWFAQIHHCQYHQPHSISHRISAIHNLCSNRLGIPALSGDT
CCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
LSLLNTPSEILNAIISDIEKAEHEINMEFYIWHHGGLADSVASALIVAAKRGVTVNVLLD
HHHHCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEC
SAGSMDFFKSHWPKLLKQSGVNIVEALSVSPFRMFFRRLDLRLHRKIVVIDNRIAYTGSM
CCCCCHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCEEEEEECEEEEECCC
NLVDPQYFKQNSGVGQWIDVMVRLTGPNVAILNTIHAWDWEVETGIRELPHLPECPASID
CCCCHHHHHCCCCCCCEEEEEEEECCCCEEEEEEEECCCCCHHHHHHHCCCCCCCCCCCC
PFNHTTQVVPSGPGMPDDIIQQVLITAICQARKSITITTPYFVPSEILLHTLRTTAHRGV
CCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCE
EVKLILPKKNDSLMVNWASKSFFETLLKAGVQIHEFNGGLLHTKSVVIDESHCIIGTVNL
EEEEEEECCCCCEEEEECHHHHHHHHHHCCCEEEECCCCEEEEEEEEEECCCEEEEEEEC
DMRSFWLNFEVSLIVDDPEFTKQLSWVLDGYLEQSTQVCFDTWRHRSWGNRFTERFFSMF
CCEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
SPLL
CCCC
>Mature Secondary Structure
MDKFYQALTIVSFVLYWLLVAGVTIRVVFKPRPVGVSLAWLMIIYILPVIGVMAYFLIGE
CCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
LNLGRTRGERARAMFTPYEQWFAQIHHCQYHQPHSISHRISAIHNLCSNRLGIPALSGDT
CCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
LSLLNTPSEILNAIISDIEKAEHEINMEFYIWHHGGLADSVASALIVAAKRGVTVNVLLD
HHHHCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEC
SAGSMDFFKSHWPKLLKQSGVNIVEALSVSPFRMFFRRLDLRLHRKIVVIDNRIAYTGSM
CCCCCHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCEEEEEECEEEEECCC
NLVDPQYFKQNSGVGQWIDVMVRLTGPNVAILNTIHAWDWEVETGIRELPHLPECPASID
CCCCHHHHHCCCCCCCEEEEEEEECCCCEEEEEEEECCCCCHHHHHHHCCCCCCCCCCCC
PFNHTTQVVPSGPGMPDDIIQQVLITAICQARKSITITTPYFVPSEILLHTLRTTAHRGV
CCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCE
EVKLILPKKNDSLMVNWASKSFFETLLKAGVQIHEFNGGLLHTKSVVIDESHCIIGTVNL
EEEEEEECCCCCEEEEECHHHHHHHHHHCCCEEEECCCCEEEEEEEEEECCCEEEEEEEC
DMRSFWLNFEVSLIVDDPEFTKQLSWVLDGYLEQSTQVCFDTWRHRSWGNRFTERFFSMF
CCEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
SPLL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA