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The map label for this gene is lpxH

Identifier: 59712209

GI number: 59712209

Start: 1797804

End: 1798529

Strand: Direct

Name: lpxH

Synonym: VF_1602

Alternate gene names: 59712209

Gene position: 1797804-1798529 (Clockwise)

Preceding gene: 59712208

Following gene: 59712210

Centisome position: 62.05

GC content: 34.3

Gene sequence:

>726_bases
ATGACTATCTTATTTATTTCTGATCTTCACTTATCCCCTCTTCGCCCTGACATCACGGATTGTTTTATTGATTTTATGCA
GAATGAAGCCATTCATGCTGAAAAACTCTACGTATTAGGCGACTTATTTGAATTTTGGATTGGTGATGATGATAATTCTC
CATTTAATGTTTCAATCAAAAATGAGTTTAAGGCATTAACGGAAAAAGGAGTCAAATGTTACTTTATTCAAGGTAATAGA
GATTTCCTGCTTAATAAACGCTTTTGTAAAGAAACAGGAGTTGAACTTTTAGATGATCACACGGTTATCGAACTTGATGG
TGAAAAAGTTCTTATTATGCACGGTGATACCTTGTGCATTGATGATATAAAATATCAAGAATTTAGAGCTAAAGTGCACA
AACCATGGCTTCAATGGGTCTTCAACCGAATTCCACTTTTCATTCGCCAGCGTATAGTAAAAAACGTTCAAGATAAGATT
AAAGAGAAAAAACAAACTAAAACTCTGTGCATCATGGATGTTACTCAAAGTGAAGTTGAACGTGTAATGCAAGAAGAAGG
TGTTCAACGTCTTATTCATGGACATACTCACCGACCAGATACGCATACCTTTATGAGCAATAATAAAGAGATGACTCGCA
TTGTATTAGGTGACTGGTATAGCCAAGGTTCAATTCTTGAGTATTCAGATAAAATCTACTCACTTCACAAAAAAGAGTTT
AAATAA

Upstream 100 bases:

>100_bases
TGAAGAAGTATTCATCACAGGCACAACAATCACAGAATAATTCTGTTTTTTTGCCAGCTTTATTCAAAGCTGGCATTTTC
TCATTACCAATAAAGTATTT

Downstream 100 bases:

>100_bases
TTATTTTTAATCGTTTGCCTTTAAATGTCTCAAACAAGAAACATAGTTTTTTTACTGTAAAGCGTTTATTATGAGCGCAG
TTCCGATTCAACACGCTGTA

Product: UDP-2,3-diacylglucosamine hydrolase

Products: 2,3-bis(3-hydroxymyristoyl)-beta-D-glucosaminyl 1; UMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MTILFISDLHLSPLRPDITDCFIDFMQNEAIHAEKLYVLGDLFEFWIGDDDNSPFNVSIKNEFKALTEKGVKCYFIQGNR
DFLLNKRFCKETGVELLDDHTVIELDGEKVLIMHGDTLCIDDIKYQEFRAKVHKPWLQWVFNRIPLFIRQRIVKNVQDKI
KEKKQTKTLCIMDVTQSEVERVMQEEGVQRLIHGHTHRPDTHTFMSNNKEMTRIVLGDWYSQGSILEYSDKIYSLHKKEF
K

Sequences:

>Translated_241_residues
MTILFISDLHLSPLRPDITDCFIDFMQNEAIHAEKLYVLGDLFEFWIGDDDNSPFNVSIKNEFKALTEKGVKCYFIQGNR
DFLLNKRFCKETGVELLDDHTVIELDGEKVLIMHGDTLCIDDIKYQEFRAKVHKPWLQWVFNRIPLFIRQRIVKNVQDKI
KEKKQTKTLCIMDVTQSEVERVMQEEGVQRLIHGHTHRPDTHTFMSNNKEMTRIVLGDWYSQGSILEYSDKIYSLHKKEF
K
>Mature_240_residues
TILFISDLHLSPLRPDITDCFIDFMQNEAIHAEKLYVLGDLFEFWIGDDDNSPFNVSIKNEFKALTEKGVKCYFIQGNRD
FLLNKRFCKETGVELLDDHTVIELDGEKVLIMHGDTLCIDDIKYQEFRAKVHKPWLQWVFNRIPLFIRQRIVKNVQDKIK
EKKQTKTLCIMDVTQSEVERVMQEEGVQRLIHGHTHRPDTHTFMSNNKEMTRIVLGDWYSQGSILEYSDKIYSLHKKEFK

Specific function: Catalyzes the hydrolysis of the pyrophosphate bond of UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1- phosphate (lipid X) and UMP

COG id: COG2908

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lpxH family

Homologues:

Organism=Escherichia coli, GI1786735, Length=229, Percent_Identity=52.4017467248908, Blast_Score=265, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPXH_VIBF1 (Q5E4E9)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_204985.1
- ProteinModelPortal:   Q5E4E9
- STRING:   Q5E4E9
- GeneID:   3278871
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1602
- NMPDR:   fig|312309.3.peg.1602
- eggNOG:   COG2908
- HOGENOM:   HBG288883
- OMA:   CHGDTLC
- PhylomeDB:   Q5E4E9
- ProtClustDB:   PRK05340
- BioCyc:   VFIS312309:VF1602-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00575
- InterPro:   IPR004843
- InterPro:   IPR010138
- TIGRFAMs:   TIGR01854

Pfam domain/function: PF00149 Metallophos

EC number: NA

Molecular weight: Translated: 28502; Mature: 28371

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILFISDLHLSPLRPDITDCFIDFMQNEAIHAEKLYVLGDLFEFWIGDDDNSPFNVSIK
CEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEH
NEFKALTEKGVKCYFIQGNRDFLLNKRFCKETGVELLDDHTVIELDGEKVLIMHGDTLCI
HHHHHHHHCCCEEEEEECCCCEECCHHHHHHCCCHHHCCCEEEEECCCEEEEEECCEEEE
DDIKYQEFRAKVHKPWLQWVFNRIPLFIRQRIVKNVQDKIKEKKQTKTLCIMDVTQSEVE
CCCHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHH
RVMQEEGVQRLIHGHTHRPDTHTFMSNNKEMTRIVLGDWYSQGSILEYSDKIYSLHKKEF
HHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHCC
K
C
>Mature Secondary Structure 
TILFISDLHLSPLRPDITDCFIDFMQNEAIHAEKLYVLGDLFEFWIGDDDNSPFNVSIK
EEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEH
NEFKALTEKGVKCYFIQGNRDFLLNKRFCKETGVELLDDHTVIELDGEKVLIMHGDTLCI
HHHHHHHHCCCEEEEEECCCCEECCHHHHHHCCCHHHCCCEEEEECCCEEEEEECCEEEE
DDIKYQEFRAKVHKPWLQWVFNRIPLFIRQRIVKNVQDKIKEKKQTKTLCIMDVTQSEVE
CCCHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHH
RVMQEEGVQRLIHGHTHRPDTHTFMSNNKEMTRIVLGDWYSQGSILEYSDKIYSLHKKEF
HHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; UDP-2,3-bis(3-hydroxymyristoyl)glucosamine [C]

Specific reaction: H2O + UDP-2,3-bis(3-hydroxymyristoyl)glucosamine = 2,3-bis(3-hydroxymyristoyl)-beta-D-glucosaminyl 1 + UMP [C]

General reaction: Lipid A biosynthesis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA