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The map label for this gene is pldA [H]

Identifier: 59712163

GI number: 59712163

Start: 1740086

End: 1740859

Strand: Reverse

Name: pldA [H]

Synonym: VF_1556

Alternate gene names: 59712163

Gene position: 1740859-1740086 (Counterclockwise)

Preceding gene: 59712164

Following gene: 172087716

Centisome position: 60.08

GC content: 34.63

Gene sequence:

>774_bases
ATGAAAAAATGGATGCTATTAATTGCATTGGCAATACCCTCTTTATCGTTCGCAAAAGAGTTAAATCGTATACATGCCTA
TGAAGATAGCTATATTTTAGGTTCTTATATTAGTGATTTGAACAAACAAACCTATATTGATGGTGGTTTTGAAGATGCTG
ATGGCCTTCATGATGTTGAAGTGAAATTCAAATTTTCATTTGCAGTACCTTTGTTCCCAGTTAGTCAAAGTTCCTCAATC
ATGTTTGCTTATACACAAAAGTCATTATGGCAGTTGGGTAACAGCGATATTTCATCCCCTTTTAGAGAAACTAACTATAA
GCCACAATTTTTCTTGATGCATCAGTCAAATATGTTGATTTTTAACAATGCTGAATTTGGTTATATGCATGAATCTAATG
GTCAAACAGCAAGTTTATCTCGTAGTTGGGATCGCATTTATGGCGCATTAGAACGAATTGATGGCCCTGTACAATATGGC
GTTAGAGCTTGGTATGTCATTGCTGATGAAGAAAATAACGAAGATATTACGGATTATTTAGGTAATTATGATGTTTGGGC
TTCTGTCGGTAATGATATTGGAACACTCAGTACTCGTTTCCATTATAATTTCGAAACCGATAAAGGTGGTGCTGAGCTTG
GTTATACATTAAACGTCAATAAATTTGTTGGTTTATACGTACAAGCATGGAAAGGGTATGGGGAAACCTTAATTGATTAT
AACCATGATCAAACTCGAATTGGTCTTGGTATTAAATTAATGCCGCCAAATTAA

Upstream 100 bases:

>100_bases
AATAGTATTAACATTATGAATGTATGAATAAAAACCTTAGTTATTAGGTATGCTTTTTGCATTAACAATAAAAAAGCAAA
TTAATGACAAAAGGTCGACT

Downstream 100 bases:

>100_bases
TGCCAGTGCTATTTATTGCGAAAAATTTAAAGCTTCTGTGATCAAGAAGCTTTTTTTATGTTTGGATAAAGGACATCTAT
GATGGCGATCATTGCGAAAA

Product: outer membrane phospholipase A

Products: NA

Alternate protein names: Detergent-resistant phospholipase A; DR-phospholipase A; Outer membrane phospholipase A; OM PLA; Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKKWMLLIALAIPSLSFAKELNRIHAYEDSYILGSYISDLNKQTYIDGGFEDADGLHDVEVKFKFSFAVPLFPVSQSSSI
MFAYTQKSLWQLGNSDISSPFRETNYKPQFFLMHQSNMLIFNNAEFGYMHESNGQTASLSRSWDRIYGALERIDGPVQYG
VRAWYVIADEENNEDITDYLGNYDVWASVGNDIGTLSTRFHYNFETDKGGAELGYTLNVNKFVGLYVQAWKGYGETLIDY
NHDQTRIGLGIKLMPPN

Sequences:

>Translated_257_residues
MKKWMLLIALAIPSLSFAKELNRIHAYEDSYILGSYISDLNKQTYIDGGFEDADGLHDVEVKFKFSFAVPLFPVSQSSSI
MFAYTQKSLWQLGNSDISSPFRETNYKPQFFLMHQSNMLIFNNAEFGYMHESNGQTASLSRSWDRIYGALERIDGPVQYG
VRAWYVIADEENNEDITDYLGNYDVWASVGNDIGTLSTRFHYNFETDKGGAELGYTLNVNKFVGLYVQAWKGYGETLIDY
NHDQTRIGLGIKLMPPN
>Mature_257_residues
MKKWMLLIALAIPSLSFAKELNRIHAYEDSYILGSYISDLNKQTYIDGGFEDADGLHDVEVKFKFSFAVPLFPVSQSSSI
MFAYTQKSLWQLGNSDISSPFRETNYKPQFFLMHQSNMLIFNNAEFGYMHESNGQTASLSRSWDRIYGALERIDGPVQYG
VRAWYVIADEENNEDITDYLGNYDVWASVGNDIGTLSTRFHYNFETDKGGAELGYTLNVNKFVGLYVQAWKGYGETLIDY
NHDQTRIGLGIKLMPPN

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=238, Percent_Identity=41.5966386554622, Blast_Score=176, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 29329; Mature: 29329

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWMLLIALAIPSLSFAKELNRIHAYEDSYILGSYISDLNKQTYIDGGFEDADGLHDVE
CCHHEEEEEHHHCCHHHHHHHHHHCCCCCCEEHHHHHHHCCCCEEECCCCCCCCCCEEEE
VKFKFSFAVPLFPVSQSSSIMFAYTQKSLWQLGNSDISSPFRETNYKPQFFLMHQSNMLI
EEEEEEEEEEEEECCCCCCEEEEEEHHHHHHCCCCCCCCCHHHCCCCCEEEEEECCCEEE
FNNAEFGYMHESNGQTASLSRSWDRIYGALERIDGPVQYGVRAWYVIADEENNEDITDYL
EECCCCCEEECCCCCEEEHHHHHHHHHHHHHHCCCCCEECCEEEEEEEECCCCCHHHHHC
GNYDVWASVGNDIGTLSTRFHYNFETDKGGAELGYTLNVNKFVGLYVQAWKGYGETLIDY
CCEEEEEECCCCCCCEEEEEEEEEECCCCCCEEEEEEECHHHEEEEEEECCCCCCEEEEC
NHDQTRIGLGIKLMPPN
CCCCEEEEEEEEEECCC
>Mature Secondary Structure
MKKWMLLIALAIPSLSFAKELNRIHAYEDSYILGSYISDLNKQTYIDGGFEDADGLHDVE
CCHHEEEEEHHHCCHHHHHHHHHHCCCCCCEEHHHHHHHCCCCEEECCCCCCCCCCEEEE
VKFKFSFAVPLFPVSQSSSIMFAYTQKSLWQLGNSDISSPFRETNYKPQFFLMHQSNMLI
EEEEEEEEEEEEECCCCCCEEEEEEHHHHHHCCCCCCCCCHHHCCCCCEEEEEECCCEEE
FNNAEFGYMHESNGQTASLSRSWDRIYGALERIDGPVQYGVRAWYVIADEENNEDITDYL
EECCCCCEEECCCCCEEEHHHHHHHHHHHHHHCCCCCEECCEEEEEEEECCCCCHHHHHC
GNYDVWASVGNDIGTLSTRFHYNFETDKGGAELGYTLNVNKFVGLYVQAWKGYGETLIDY
CCEEEEEECCCCCCCEEEEEEEEEECCCCCCEEEEEEECHHHEEEEEEECCCCCCEEEEC
NHDQTRIGLGIKLMPPN
CCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8300539 [H]