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The map label for this gene is nrfG [H]

Identifier: 59712162

GI number: 59712162

Start: 1739289

End: 1739936

Strand: Reverse

Name: nrfG [H]

Synonym: VF_1555

Alternate gene names: 59712162

Gene position: 1739936-1739289 (Counterclockwise)

Preceding gene: 172087716

Following gene: 59712160

Centisome position: 60.05

GC content: 36.88

Gene sequence:

>648_bases
ATGATTATAACGATGTTACTAAGTTTTGTTCTTATTGGGGCAGTATTATGGTGGTTTTATAGCCGTGGAAATGCCGCTTC
TTTGAATCCTGTATGGATGATATTTGTTGTTGTAGTCAGTGTGATTGTTGTATTTTCATCTGGATTAAGACCAGAAAAAT
ACGCAGTACAAAATGAGTTAATTACAGAGCCACTAGGTCCTCTCTCTTATGAAGATAAGATGCGCTATTTAGAGGATCAA
CTGAAACAGAGCCCAAATGACGCTGAATTGTGGTTTGAAATAGGGCAGGGTTATCTACTGAATGGTGAATTAAACAATGC
GAATATCTGCTTTGGATACGCAATTCGTTTAACAGAAGAGCCAACAGCCAATCAATATGCAGCAAAAGCGACGACTCAGT
ATTATTTACATTCTCAGATTATGAGCGATGAAGTAGAAGAGTTACTTAAGAAAGCGTTAGTTCTTGATGAATATAATCAA
GCCGCTTTAACATTAATTGCTTCTGATCATTTTGTTACTTTCCGTTACCAGAAGGCCATTAATGCGTGGCAGAAAATTTT
AGATTCTGAGCGAGTTGATGTTGATCGAGTGACAATTATTAACTCAATTAATGAAGCCAAACAGTTAATGCACGCACGCC
GTAATTAA

Upstream 100 bases:

>100_bases
CTTTTTTTATGTTTGGATAAAGGACATCTATGATGGCGATCATTGCGAAAAATGATGATTTCAATTACGATCTGGCCGAT
TTTTTATTTGGATACTCAGT

Downstream 100 bases:

>100_bases
ACTTTATAAGTTGTACTAATAAATAAGAAACATAAAAAAGCCGACTTAACTGTCGGCTTTTTATTTATTACTACCTGTTT
AAGCTAGTCGTAATTACATT

Product: heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MIITMLLSFVLIGAVLWWFYSRGNAASLNPVWMIFVVVVSVIVVFSSGLRPEKYAVQNELITEPLGPLSYEDKMRYLEDQ
LKQSPNDAELWFEIGQGYLLNGELNNANICFGYAIRLTEEPTANQYAAKATTQYYLHSQIMSDEVEELLKKALVLDEYNQ
AALTLIASDHFVTFRYQKAINAWQKILDSERVDVDRVTIINSINEAKQLMHARRN

Sequences:

>Translated_215_residues
MIITMLLSFVLIGAVLWWFYSRGNAASLNPVWMIFVVVVSVIVVFSSGLRPEKYAVQNELITEPLGPLSYEDKMRYLEDQ
LKQSPNDAELWFEIGQGYLLNGELNNANICFGYAIRLTEEPTANQYAAKATTQYYLHSQIMSDEVEELLKKALVLDEYNQ
AALTLIASDHFVTFRYQKAINAWQKILDSERVDVDRVTIINSINEAKQLMHARRN
>Mature_215_residues
MIITMLLSFVLIGAVLWWFYSRGNAASLNPVWMIFVVVVSVIVVFSSGLRPEKYAVQNELITEPLGPLSYEDKMRYLEDQ
LKQSPNDAELWFEIGQGYLLNGELNNANICFGYAIRLTEEPTANQYAAKATTQYYLHSQIMSDEVEELLKKALVLDEYNQ
AALTLIASDHFVTFRYQKAINAWQKILDSERVDVDRVTIINSINEAKQLMHARRN

Specific function: Required for formate-dependent nitrite reduction. Not required for the biosynthesis of any of the c-type cytochromes nor for the secretion of the periplasmic cytochromes [H]

COG id: COG4235

COG function: function code O; Cytochrome c biogenesis factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 TPR repeats [H]

Homologues:

Organism=Escherichia coli, GI1790513, Length=180, Percent_Identity=32.7777777777778, Blast_Score=91, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013026
- InterPro:   IPR011990
- InterPro:   IPR019734 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24648; Mature: 24648

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50005 TPR ; PS50293 TPR_REGION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIITMLLSFVLIGAVLWWFYSRGNAASLNPVWMIFVVVVSVIVVFSSGLRPEKYAVQNEL
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ITEPLGPLSYEDKMRYLEDQLKQSPNDAELWFEIGQGYLLNGELNNANICFGYAIRLTEE
HHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCCCCEEEEEEEEEEECC
PTANQYAAKATTQYYLHSQIMSDEVEELLKKALVLDEYNQAALTLIASDHFVTFRYQKAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEHHHHHHH
NAWQKILDSERVDVDRVTIINSINEAKQLMHARRN
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIITMLLSFVLIGAVLWWFYSRGNAASLNPVWMIFVVVVSVIVVFSSGLRPEKYAVQNEL
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ITEPLGPLSYEDKMRYLEDQLKQSPNDAELWFEIGQGYLLNGELNNANICFGYAIRLTEE
HHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCCCCEEEEEEEEEEECC
PTANQYAAKATTQYYLHSQIMSDEVEELLKKALVLDEYNQAALTLIASDHFVTFRYQKAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEHHHHHHH
NAWQKILDSERVDVDRVTIINSINEAKQLMHARRN
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]