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The map label for this gene is kefA [H]

Identifier: 59712146

GI number: 59712146

Start: 1722593

End: 1723468

Strand: Direct

Name: kefA [H]

Synonym: VF_1539

Alternate gene names: 59712146

Gene position: 1722593-1723468 (Clockwise)

Preceding gene: 59712145

Following gene: 59712147

Centisome position: 59.45

GC content: 37.79

Gene sequence:

>876_bases
ATGCTTCGTATTTTTATATTTTTTATAACGCTCGGATTTGGTTCGCTTGCATTTGCAGAAGAAGCACAATCTATCGACAA
CTTACAGCAGTTCGCTAGTCTAATTCGTTGGAGCGGTGTTGCTTTCTCATTTGTTATCGTATTTGTTGCTTGGTTACTGT
TACGCTTTACCCAATCAATTGTAGATGGAATAAGTAGTCAATTTGCTCAACGTCGAATGTTGATGCAAAAACTGCAATCT
TTCTTTCAATTCTTTATTTATATGTCAACGGGTATGGCTGTTTTCATGCTTAGCTTCCGTGTTGATGAGCGTGTGTTAGC
ACTAATCGGTGGTACGATTGCTGTCTCTGTTGGCTTTGCACTGAAAGACTTAACTGCATCATTCATCGCTGGATTAACAG
TAATGATCGATCGCCCTTTCCAAGTCGGTGATCGCGTAACGTTTGAAGGTAATTATGGAGACATTATCGCTATTGGTTTA
CGTTCTGTTCGAATGCAAACTCTAAACGATGACATTATTACTATCCCTAACAACAAATTCTTAAATGAAGTTGCTGTAAG
TGGAAACTATGGTGCATTAGATATGCAAGTTGTTATTCCATTTTATATTGGTATGGACCAAGACATTAATACCGCAAGAG
ATATTATTCAAGAAGCGGCATCTTCTAGTCGTTATATCCACTTACCTAAGCCTGTGGTTGTACTAGTGAAGCAAACCATT
ACAGATAATTATTTAGCCATTCAATTAACTTGTAAGGCTTACGTGGTAGATATTAAGTTTGAAAAGCTGTTCGAAACGGA
TATCACCTTACGTGTTATGCAAGAATTCAGAAAATCAGACATATTCCCGCCAGCCATTGCCGTTCAAAACAAATAG

Upstream 100 bases:

>100_bases
GGCTACATTGAATGGAGTGATGATAAAGCAAGGATCTCTGATCACTGGTTCCGCCACATTACTAATGTTCTTCATCGTCA
ACATTTATTGGTGAAATAAT

Downstream 100 bases:

>100_bases
TTATTCTATTAAAGGTAGCATGATAAACCATGCTACCTCTCTCTTTTCTCTCTTGAGTAATTAACTTATCGCTCGCCGTA
CTGTGCTTTTATTGCTAACA

Product: transporter

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MLRIFIFFITLGFGSLAFAEEAQSIDNLQQFASLIRWSGVAFSFVIVFVAWLLLRFTQSIVDGISSQFAQRRMLMQKLQS
FFQFFIYMSTGMAVFMLSFRVDERVLALIGGTIAVSVGFALKDLTASFIAGLTVMIDRPFQVGDRVTFEGNYGDIIAIGL
RSVRMQTLNDDIITIPNNKFLNEVAVSGNYGALDMQVVIPFYIGMDQDINTARDIIQEAASSSRYIHLPKPVVVLVKQTI
TDNYLAIQLTCKAYVVDIKFEKLFETDITLRVMQEFRKSDIFPPAIAVQNK

Sequences:

>Translated_291_residues
MLRIFIFFITLGFGSLAFAEEAQSIDNLQQFASLIRWSGVAFSFVIVFVAWLLLRFTQSIVDGISSQFAQRRMLMQKLQS
FFQFFIYMSTGMAVFMLSFRVDERVLALIGGTIAVSVGFALKDLTASFIAGLTVMIDRPFQVGDRVTFEGNYGDIIAIGL
RSVRMQTLNDDIITIPNNKFLNEVAVSGNYGALDMQVVIPFYIGMDQDINTARDIIQEAASSSRYIHLPKPVVVLVKQTI
TDNYLAIQLTCKAYVVDIKFEKLFETDITLRVMQEFRKSDIFPPAIAVQNK
>Mature_291_residues
MLRIFIFFITLGFGSLAFAEEAQSIDNLQQFASLIRWSGVAFSFVIVFVAWLLLRFTQSIVDGISSQFAQRRMLMQKLQS
FFQFFIYMSTGMAVFMLSFRVDERVLALIGGTIAVSVGFALKDLTASFIAGLTVMIDRPFQVGDRVTFEGNYGDIIAIGL
RSVRMQTLNDDIITIPNNKFLNEVAVSGNYGALDMQVVIPFYIGMDQDINTARDIIQEAASSSRYIHLPKPVVVLVKQTI
TDNYLAIQLTCKAYVVDIKFEKLFETDITLRVMQEFRKSDIFPPAIAVQNK

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=194, Percent_Identity=24.2268041237113, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 32807; Mature: 32807

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIFIFFITLGFGSLAFAEEAQSIDNLQQFASLIRWSGVAFSFVIVFVAWLLLRFTQSI
CCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VDGISSQFAQRRMLMQKLQSFFQFFIYMSTGMAVFMLSFRVDERVLALIGGTIAVSVGFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LKDLTASFIAGLTVMIDRPFQVGDRVTFEGNYGDIIAIGLRSVRMQTLNDDIITIPNNKF
HHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCEEEEEHHHHHHHHCCCCEEECCCCHH
LNEVAVSGNYGALDMQVVIPFYIGMDQDINTARDIIQEAASSSRYIHLPKPVVVLVKQTI
HHHHEECCCCCCEEHEEEEEEHCCCCCCCHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
TDNYLAIQLTCKAYVVDIKFEKLFETDITLRVMQEFRKSDIFPPAIAVQNK
CCCEEEEEEEEEEEEEEEEHHHHHHCCHHHHHHHHHHHCCCCCCCEEECCC
>Mature Secondary Structure
MLRIFIFFITLGFGSLAFAEEAQSIDNLQQFASLIRWSGVAFSFVIVFVAWLLLRFTQSI
CCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VDGISSQFAQRRMLMQKLQSFFQFFIYMSTGMAVFMLSFRVDERVLALIGGTIAVSVGFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LKDLTASFIAGLTVMIDRPFQVGDRVTFEGNYGDIIAIGLRSVRMQTLNDDIITIPNNKF
HHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCEEEEEHHHHHHHHCCCCEEECCCCHH
LNEVAVSGNYGALDMQVVIPFYIGMDQDINTARDIIQEAASSSRYIHLPKPVVVLVKQTI
HHHHEECCCCCCEEHEEEEEEHCCCCCCCHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
TDNYLAIQLTCKAYVVDIKFEKLFETDITLRVMQEFRKSDIFPPAIAVQNK
CCCEEEEEEEEEEEEEEEEHHHHHHCCHHHHHHHHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]