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The map label for this gene is rlmI [H]

Identifier: 59712112

GI number: 59712112

Start: 1667032

End: 1668222

Strand: Direct

Name: rlmI [H]

Synonym: VF_1505

Alternate gene names: 59712112

Gene position: 1667032-1668222 (Clockwise)

Preceding gene: 59712111

Following gene: 59712114

Centisome position: 57.53

GC content: 39.71

Gene sequence:

>1191_bases
ATGACTGTTTCAATTTATTTAGCAAAAGGACGTGATAAAGCATTGCGTCGTCGTCACCCTTGGATCTTCTCTCGCGGTAT
TGACCGTATCGATGGTAAAGCAGAACTAGGTGAAACAGTTGAGATTTATGCAAACAACGGCGAATGGCTTGCTCGTGGTG
CGTTTTCTCCACAGTCTCAGATCCGTGCTCGTGTTTGGACTTTTGATAAAAACGAAGCGATTGATAAAGATTTCTTTGTT
CGTAAATTAAATCAAGCTCAAGCATTACGTAATGTATTAGCTGAGCGTGATGGTTTAACTGGTTATCGTTTAATTGCTGC
TGAATCAGATGGCTTACCGGGCATCACGATTGACCGTTACCAAGATTACTTTGTGTGCCAATTACTAAGTGCTGGCGCAG
AAGCAACGAAAGAGCTATTAGTTGAAGCATTAGTTGAATGCTACCCTGACTGTAATATTTACGAGCGCTCAGATGTGTCA
GTTCGTAAAAAAGAAGGTCTAAAACAACGTACAGGTGTTCTACACGGTGAAGAGCCACCAGAGTCAGTAGTTATTGAAGA
GAATGGCGTGAAAATTAGCGTTGATATCGTAAATGGCCATAAAACAGGTTTCTATCTTGACCAACGTGATAGCCGTGAAC
GTGCTTGTAAATACGTAAAAAATAAATCAGTTCTAAACTGTTTCTCTTATACTGGTGGTTTTGGTTTATACGCTCTAAAA
GGCGGCGCAAAACACGTGATTAATGCGGATGTATCTCAACTTGCTTTAGACACAGCGAAATACAATGCTGAGATCAATAA
ATTTGATTTATCTAAAGCTGAATTCTTAAATGCAGATGTGTTTAAGTTATTGCGTGAATACCGTGATAACGGTACTAAAT
TTGATGTTGTTATTATGGATCCACCTAAATTTGCTGAATCTAAAAATCAGCTGACAGGTGCATGCCGTGGCTATAAAGAC
ATTAACATGTTAGCAATGCAAATTCTAAACCCAGGCGGTACGCTATTAACTTACTCATGTTCTGGTTTAATGGATGCGGG
TTTATTCCAAAAAATTGTTGCTGATGCTGCACTAGATGCTCATCGTACGGTTCAGTTTATTGAACGCTTTGAACAAGCAG
CGGATCATCCTTTAGATTCTGCTTACCCTGAGGGTTTCTACCTTAAAGGTTTTGCTTGTCGAGTTGTATAG

Upstream 100 bases:

>100_bases
TCTATTCCCCTTTCCTCACTATATAAATCGTTTTATTCATTGCTTTTATGACACGCATCACAGAGAATATCGGCTCACTT
TTTATCAACAGAGCATCAAT

Downstream 100 bases:

>100_bases
CACTCACTTTAATTATTAAGCTATTGATAGCCAATTAAAAATCAATAAAAAAGGCGAGCAGGTATTACCTACTCGCCTTT
TTATATTCACTTAAACGTTA

Product: methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTVSIYLAKGRDKALRRRHPWIFSRGIDRIDGKAELGETVEIYANNGEWLARGAFSPQSQIRARVWTFDKNEAIDKDFFV
RKLNQAQALRNVLAERDGLTGYRLIAAESDGLPGITIDRYQDYFVCQLLSAGAEATKELLVEALVECYPDCNIYERSDVS
VRKKEGLKQRTGVLHGEEPPESVVIEENGVKISVDIVNGHKTGFYLDQRDSRERACKYVKNKSVLNCFSYTGGFGLYALK
GGAKHVINADVSQLALDTAKYNAEINKFDLSKAEFLNADVFKLLREYRDNGTKFDVVIMDPPKFAESKNQLTGACRGYKD
INMLAMQILNPGGTLLTYSCSGLMDAGLFQKIVADAALDAHRTVQFIERFEQAADHPLDSAYPEGFYLKGFACRVV

Sequences:

>Translated_396_residues
MTVSIYLAKGRDKALRRRHPWIFSRGIDRIDGKAELGETVEIYANNGEWLARGAFSPQSQIRARVWTFDKNEAIDKDFFV
RKLNQAQALRNVLAERDGLTGYRLIAAESDGLPGITIDRYQDYFVCQLLSAGAEATKELLVEALVECYPDCNIYERSDVS
VRKKEGLKQRTGVLHGEEPPESVVIEENGVKISVDIVNGHKTGFYLDQRDSRERACKYVKNKSVLNCFSYTGGFGLYALK
GGAKHVINADVSQLALDTAKYNAEINKFDLSKAEFLNADVFKLLREYRDNGTKFDVVIMDPPKFAESKNQLTGACRGYKD
INMLAMQILNPGGTLLTYSCSGLMDAGLFQKIVADAALDAHRTVQFIERFEQAADHPLDSAYPEGFYLKGFACRVV
>Mature_395_residues
TVSIYLAKGRDKALRRRHPWIFSRGIDRIDGKAELGETVEIYANNGEWLARGAFSPQSQIRARVWTFDKNEAIDKDFFVR
KLNQAQALRNVLAERDGLTGYRLIAAESDGLPGITIDRYQDYFVCQLLSAGAEATKELLVEALVECYPDCNIYERSDVSV
RKKEGLKQRTGVLHGEEPPESVVIEENGVKISVDIVNGHKTGFYLDQRDSRERACKYVKNKSVLNCFSYTGGFGLYALKG
GAKHVINADVSQLALDTAKYNAEINKFDLSKAEFLNADVFKLLREYRDNGTKFDVVIMDPPKFAESKNQLTGACRGYKDI
NMLAMQILNPGGTLLTYSCSGLMDAGLFQKIVADAALDAHRTVQFIERFEQAADHPLDSAYPEGFYLKGFACRVV

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=396, Percent_Identity=65.1515151515152, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI1787181, Length=248, Percent_Identity=26.6129032258064, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44349; Mature: 44218

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSIYLAKGRDKALRRRHPWIFSRGIDRIDGKAELGETVEIYANNGEWLARGAFSPQSQ
CEEEEEEECCCHHHHHHCCCCHHHCCCCCCCCCHHCCCEEEEEECCCCEEEECCCCCHHH
IRARVWTFDKNEAIDKDFFVRKLNQAQALRNVLAERDGLTGYRLIAAESDGLPGITIDRY
HEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHH
QDYFVCQLLSAGAEATKELLVEALVECYPDCNIYERSDVSVRKKEGLKQRTGVLHGEEPP
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHCCHHHHHCCCCCCCCC
ESVVIEENGVKISVDIVNGHKTGFYLDQRDSRERACKYVKNKSVLNCFSYTGGFGLYALK
CEEEEECCCEEEEEEEECCCCCCEEECCCCCHHHHHHHHHCCCCEEHHHCCCCCEEEEEC
GGAKHVINADVSQLALDTAKYNAEINKFDLSKAEFLNADVFKLLREYRDNGTKFDVVIMD
CCCCEECCCCHHHHHHHHHHHCCCCCEECCCHHHHCCHHHHHHHHHHHCCCCEEEEEEEC
PPKFAESKNQLTGACRGYKDINMLAMQILNPGGTLLTYSCSGLMDAGLFQKIVADAALDA
CCCHHCCHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
HRTVQFIERFEQAADHPLDSAYPEGFYLKGFACRVV
HHHHHHHHHHHHHHCCCCCCCCCCCEEECCEEEEEC
>Mature Secondary Structure 
TVSIYLAKGRDKALRRRHPWIFSRGIDRIDGKAELGETVEIYANNGEWLARGAFSPQSQ
EEEEEEECCCHHHHHHCCCCHHHCCCCCCCCCHHCCCEEEEEECCCCEEEECCCCCHHH
IRARVWTFDKNEAIDKDFFVRKLNQAQALRNVLAERDGLTGYRLIAAESDGLPGITIDRY
HEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHH
QDYFVCQLLSAGAEATKELLVEALVECYPDCNIYERSDVSVRKKEGLKQRTGVLHGEEPP
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHCCHHHHHCCCCCCCCC
ESVVIEENGVKISVDIVNGHKTGFYLDQRDSRERACKYVKNKSVLNCFSYTGGFGLYALK
CEEEEECCCEEEEEEEECCCCCCEEECCCCCHHHHHHHHHCCCCEEHHHCCCCCEEEEEC
GGAKHVINADVSQLALDTAKYNAEINKFDLSKAEFLNADVFKLLREYRDNGTKFDVVIMD
CCCCEECCCCHHHHHHHHHHHCCCCCEECCCHHHHCCHHHHHHHHHHHCCCCEEEEEEEC
PPKFAESKNQLTGACRGYKDINMLAMQILNPGGTLLTYSCSGLMDAGLFQKIVADAALDA
CCCHHCCHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
HRTVQFIERFEQAADHPLDSAYPEGFYLKGFACRVV
HHHHHHHHHHHHHHCCCCCCCCCCCEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA