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The map label for this gene is helD [H]

Identifier: 59712071

GI number: 59712071

Start: 1621074

End: 1622471

Strand: Direct

Name: helD [H]

Synonym: VF_1464

Alternate gene names: 59712071

Gene position: 1621074-1622471 (Clockwise)

Preceding gene: 59712066

Following gene: 59712072

Centisome position: 55.95

GC content: 37.98

Gene sequence:

>1398_bases
ATGACTTCATGGATTCTTTCAACAAACCCGATTGCTCGTCTCTTTAAAAAAGAAGCGGTTGTAACAATAGATAATGATGG
AATACGTATTGTTCAGGCCGAAAATGAAACCATCATTAAATGGGATCAACTAGATTCCCCACCCAATCTTTCACTTTCTA
TTGATGGCGGTTGTTTAACGTTTATCTCGCAAGGAAAAAACCATCGCTACCGAATGTTGGGATATTTTACGCCTTTTAAA
TACGCCAAGCGTTTCTTTCCCTTCTGGGCGAATCAAAATGCAGAACGTTTACAAGATTTTCTCAGTGATGCTTACATTCA
GTGCACATCAACGTTTCTTAGAGACTCTTCGATAGAAAATATCCGTGCAGTAGTACGTAATGAAATTAAACGATGGAAAG
GTTGGGATAAGGTTGAAGGATTATCTGAGCTTGCGCATAAAACGGTAACTCAACTTACCAATATTCATCATTGGTCGTCA
GCTGATATTGAGAAGATTCGCCAACGCTATGTGAACAAGCAATTAGCTCAATACCAAACCTTTTTTGATAGCGTTGAATC
TAACCCTTTAACCAATAGACAAAGAATCGCTTGTGTTACTGATAATGACAACAACCTCTTACTTGCTGGCGCAGGAACAG
GTAAAACAAGCGTTATGATCGGTAAAGCTGGGTATTTGGTTAATAGCGGTAAAGCAAGCCCAAAGCAGATCTTAATGCTC
GCTTATGGTCGAATTGCTGCTCAAGAAATGAATGAGCGAATCAAAGAAAAACTGGGCTTTGATGATGTAAAAGCGAGTAC
CTTTCACAGCCTTGGTGTAAAAATAATCTCTGAAGTAGAAGGCAAAGCCCCTAGCCTATCTAAGCTTGAAGATAATCCAA
AAGTTAAAGCCAAATGGATGCACGATGAGATTGAAATTCTTATGCGTGATAATAACTATAGAAAGGCGTTACTTGATTAT
TTTAGTTCTTATTATTTCGTTGATAAAAACCCGTGGGAATTTGAATCTCAAGGTGCTTACCTTAAATATCTAAATGATAA
TGAAGTTCAAACCATGAATGGCGAGAAGGTAAAAAGCTACGGTGAGCTTGTTGTGGCCAATTGGTTATTTAGAAAAGGAA
TTAAGTATCAATATGAAGCCAAATACCGCTTTGATGTAGCTACAGAGAAGTACCGTCAATACGAGCCTGATTTTTATCTC
CCTGATTATGATATCTACATTGAATATTACGGTACTGATGAAAATGGGGATACCGCACCATACATAAATAGAGAACGCTA
TCAGCAAGGTATTGAATGGAAACGCAAGACACATAAGCAATATGGCACAGGTTATGTTGAGGTTTTTTACCATCAGCATA
AAAAAAGAAAACTGCCACAAGCACTTGAGAAAGAGTGA

Upstream 100 bases:

>100_bases
AATTCACAAAAGTTAACTTGGCTCAAATACAGTCCGTTATTTATTACGCTAGACTACAGCTAATTTTCGAGTCACTTCAC
ACTTTATCTGGCCGTACATA

Downstream 100 bases:

>100_bases
CTCGCTCGAGGCGTAAAATTAGAGCCTGTCTCTGATAACGCCTTACTTGATAATTTAGAACAACTGGGACAAGTAACAGA
GTTAGCAAAATTATTAGGTG

Product: superfamily I DNA/RNA helicase

Products: NA

Alternate protein names: 75 kDa helicase [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MTSWILSTNPIARLFKKEAVVTIDNDGIRIVQAENETIIKWDQLDSPPNLSLSIDGGCLTFISQGKNHRYRMLGYFTPFK
YAKRFFPFWANQNAERLQDFLSDAYIQCTSTFLRDSSIENIRAVVRNEIKRWKGWDKVEGLSELAHKTVTQLTNIHHWSS
ADIEKIRQRYVNKQLAQYQTFFDSVESNPLTNRQRIACVTDNDNNLLLAGAGTGKTSVMIGKAGYLVNSGKASPKQILML
AYGRIAAQEMNERIKEKLGFDDVKASTFHSLGVKIISEVEGKAPSLSKLEDNPKVKAKWMHDEIEILMRDNNYRKALLDY
FSSYYFVDKNPWEFESQGAYLKYLNDNEVQTMNGEKVKSYGELVVANWLFRKGIKYQYEAKYRFDVATEKYRQYEPDFYL
PDYDIYIEYYGTDENGDTAPYINRERYQQGIEWKRKTHKQYGTGYVEVFYHQHKKRKLPQALEKE

Sequences:

>Translated_465_residues
MTSWILSTNPIARLFKKEAVVTIDNDGIRIVQAENETIIKWDQLDSPPNLSLSIDGGCLTFISQGKNHRYRMLGYFTPFK
YAKRFFPFWANQNAERLQDFLSDAYIQCTSTFLRDSSIENIRAVVRNEIKRWKGWDKVEGLSELAHKTVTQLTNIHHWSS
ADIEKIRQRYVNKQLAQYQTFFDSVESNPLTNRQRIACVTDNDNNLLLAGAGTGKTSVMIGKAGYLVNSGKASPKQILML
AYGRIAAQEMNERIKEKLGFDDVKASTFHSLGVKIISEVEGKAPSLSKLEDNPKVKAKWMHDEIEILMRDNNYRKALLDY
FSSYYFVDKNPWEFESQGAYLKYLNDNEVQTMNGEKVKSYGELVVANWLFRKGIKYQYEAKYRFDVATEKYRQYEPDFYL
PDYDIYIEYYGTDENGDTAPYINRERYQQGIEWKRKTHKQYGTGYVEVFYHQHKKRKLPQALEKE
>Mature_464_residues
TSWILSTNPIARLFKKEAVVTIDNDGIRIVQAENETIIKWDQLDSPPNLSLSIDGGCLTFISQGKNHRYRMLGYFTPFKY
AKRFFPFWANQNAERLQDFLSDAYIQCTSTFLRDSSIENIRAVVRNEIKRWKGWDKVEGLSELAHKTVTQLTNIHHWSSA
DIEKIRQRYVNKQLAQYQTFFDSVESNPLTNRQRIACVTDNDNNLLLAGAGTGKTSVMIGKAGYLVNSGKASPKQILMLA
YGRIAAQEMNERIKEKLGFDDVKASTFHSLGVKIISEVEGKAPSLSKLEDNPKVKAKWMHDEIEILMRDNNYRKALLDYF
SSYYFVDKNPWEFESQGAYLKYLNDNEVQTMNGEKVKSYGELVVANWLFRKGIKYQYEAKYRFDVATEKYRQYEPDFYLP
DYDIYIEYYGTDENGDTAPYINRERYQQGIEWKRKTHKQYGTGYVEVFYHQHKKRKLPQALEKE

Specific function: Helicase IV catalyzes the unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand in a reaction that is dependent upon the hydrolysis of ATP [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase ATP-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787196, Length=139, Percent_Identity=47.4820143884892, Blast_Score=131, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000212
- InterPro:   IPR014016
- InterPro:   IPR022161
- InterPro:   IPR003661 [H]

Pfam domain/function: PF12462 Nucleolin_N; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54298; Mature: 54167

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSWILSTNPIARLFKKEAVVTIDNDGIRIVQAENETIIKWDQLDSPPNLSLSIDGGCLT
CCCEEECCCHHHHHHHCCEEEEECCCCEEEEEECCCEEEEECCCCCCCCEEEEECCCEEE
FISQGKNHRYRMLGYFTPFKYAKRFFPFWANQNAERLQDFLSDAYIQCTSTFLRDSSIEN
EHHCCCCCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
IRAVVRNEIKRWKGWDKVEGLSELAHKTVTQLTNIHHWSSADIEKIRQRYVNKQLAQYQT
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
FFDSVESNPLTNRQRIACVTDNDNNLLLAGAGTGKTSVMIGKAGYLVNSGKASPKQILML
HHHHHCCCCCCCCCEEEEEEECCCCEEEEECCCCCCEEEEECCCEEEECCCCCHHHHHHH
AYGRIAAQEMNERIKEKLGFDDVKASTFHSLGVKIISEVEGKAPSLSKLEDNPKVKAKWM
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEE
HDEIEILMRDNNYRKALLDYFSSYYFVDKNPWEFESQGAYLKYLNDNEVQTMNGEKVKSY
CCEEEEEEECCCHHHHHHHHHHCEEEECCCCCCCCCCCCEEEEECCCCEEECCCHHHHHH
GELVVANWLFRKGIKYQYEAKYRFDVATEKYRQYEPDFYLPDYDIYIEYYGTDENGDTAP
HHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHCCCCCEECCCEEEEEEEEECCCCCCCCC
YINRERYQQGIEWKRKTHKQYGTGYVEVFYHQHKKRKLPQALEKE
CCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCHHHHCCC
>Mature Secondary Structure 
TSWILSTNPIARLFKKEAVVTIDNDGIRIVQAENETIIKWDQLDSPPNLSLSIDGGCLT
CCEEECCCHHHHHHHCCEEEEECCCCEEEEEECCCEEEEECCCCCCCCEEEEECCCEEE
FISQGKNHRYRMLGYFTPFKYAKRFFPFWANQNAERLQDFLSDAYIQCTSTFLRDSSIEN
EHHCCCCCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
IRAVVRNEIKRWKGWDKVEGLSELAHKTVTQLTNIHHWSSADIEKIRQRYVNKQLAQYQT
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
FFDSVESNPLTNRQRIACVTDNDNNLLLAGAGTGKTSVMIGKAGYLVNSGKASPKQILML
HHHHHCCCCCCCCCEEEEEEECCCCEEEEECCCCCCEEEEECCCEEEECCCCCHHHHHHH
AYGRIAAQEMNERIKEKLGFDDVKASTFHSLGVKIISEVEGKAPSLSKLEDNPKVKAKWM
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEE
HDEIEILMRDNNYRKALLDYFSSYYFVDKNPWEFESQGAYLKYLNDNEVQTMNGEKVKSY
CCEEEEEEECCCHHHHHHHHHHCEEEECCCCCCCCCCCCEEEEECCCCEEECCCHHHHHH
GELVVANWLFRKGIKYQYEAKYRFDVATEKYRQYEPDFYLPDYDIYIEYYGTDENGDTAP
HHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHCCCCCEECCCEEEEEEEEECCCCCCCCC
YINRERYQQGIEWKRKTHKQYGTGYVEVFYHQHKKRKLPQALEKE
CCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2542273; 8905232; 9278503 [H]