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The map label for this gene is rbsR [H]

Identifier: 59712056

GI number: 59712056

Start: 1599955

End: 1600962

Strand: Direct

Name: rbsR [H]

Synonym: VF_1449

Alternate gene names: 59712056

Gene position: 1599955-1600962 (Clockwise)

Preceding gene: 59712055

Following gene: 59712058

Centisome position: 55.22

GC content: 40.48

Gene sequence:

>1008_bases
ATGGCAACAATCAAAGATGTAGCCAAACATGCAAGTGTCTCAACCTCAACAGTAAGTCATGTTTTAAATAAAACTCGATT
TGTTAGCGAAGATATTTCAGAACGTGTCATGGCCGCAGTAGCAGAACTAAACTATGCGCCTTCTGCCCTCGCCCGCAGTT
TAAAAGTCAATCATACAAAAACCTTTGGCATGTTAGTGACAACCTCAACGAACCCATTTTTTGGTGAAGTGGTAAAAGGG
GTAGAACGTCGCTGCTATGAAAAAGGCTATAACCTCATCTTATGTAATACCGAAGGTGACGCAGAGCGTGTTCATTCAAG
CTTAGATACCTTGCTACAAAAGCGTGTCGATGGCTTGTTACTGATGTGTACCGAAATCGAAAATCAAGTGCTTGAGCTCT
TTTCTCGTTATCAACCGGTTCCTACCGTTGTAATGGATTGGGGATTGATTGATTTTCCAAGCGACAAAATTCAAGACAAC
TCGCACCACGGTGGCTATCTGGCAACAAAACACCTTATTGAACAAGGTCACACTGAAATTGGTTGTTTAACTGGTCCTCT
CAATAAGTTGCAAGCACAACAACGTTTAAGTGGTTTTGTGCAAGCAATGGAAGAGTCAGGATTAGAAATCAATAAAAACT
GGATTGCATCAGGCAATTTTGAATGTGAAGGCGGTGAAGCGGCTTTCAACGAAATTCATGCTAAAGGCAAACTACCAACG
GCATTATTTGTTTGTAATGACATGATGGCAATGGGAATGATCAATTGCGCAAGTAAAAAAGGCATTTCAGTTCCTAATGA
CATTTCAATTGTTGGTTATGATGACATTAAAATGGCTAAATACATCACGCCATCACTTACTACCATTCATCAACCAAAAC
ACCGATTAGGCCAACAAGCGGTCGATTCACTACTAGAAAAAATAGCAACAAAATCAGAAACGAATCGAGTTTTTCAGCTA
GAGCCTACACTCATGGTTCGAGACAGCGTAAAGACGCTATCAAATTAG

Upstream 100 bases:

>100_bases
TCACTGAAGTCGAACGTTTTCTCTTAGAGCATTGATAATTAAATATCGTTGCCTGTATTTGTATTTAATGTAGGCAACGA
TATAAACAAGGATTGAATTT

Downstream 100 bases:

>100_bases
AACCATCACTGAATAAAAAATAAAGCAGAACAATAGAGCCTCTCATCATATTCCTTTCTAATGGAAGATAATAAGAGGCT
CTTTCTATTCTAATAATTAT

Product: transcriptional repressor RbsR

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MATIKDVAKHASVSTSTVSHVLNKTRFVSEDISERVMAAVAELNYAPSALARSLKVNHTKTFGMLVTTSTNPFFGEVVKG
VERRCYEKGYNLILCNTEGDAERVHSSLDTLLQKRVDGLLLMCTEIENQVLELFSRYQPVPTVVMDWGLIDFPSDKIQDN
SHHGGYLATKHLIEQGHTEIGCLTGPLNKLQAQQRLSGFVQAMEESGLEINKNWIASGNFECEGGEAAFNEIHAKGKLPT
ALFVCNDMMAMGMINCASKKGISVPNDISIVGYDDIKMAKYITPSLTTIHQPKHRLGQQAVDSLLEKIATKSETNRVFQL
EPTLMVRDSVKTLSN

Sequences:

>Translated_335_residues
MATIKDVAKHASVSTSTVSHVLNKTRFVSEDISERVMAAVAELNYAPSALARSLKVNHTKTFGMLVTTSTNPFFGEVVKG
VERRCYEKGYNLILCNTEGDAERVHSSLDTLLQKRVDGLLLMCTEIENQVLELFSRYQPVPTVVMDWGLIDFPSDKIQDN
SHHGGYLATKHLIEQGHTEIGCLTGPLNKLQAQQRLSGFVQAMEESGLEINKNWIASGNFECEGGEAAFNEIHAKGKLPT
ALFVCNDMMAMGMINCASKKGISVPNDISIVGYDDIKMAKYITPSLTTIHQPKHRLGQQAVDSLLEKIATKSETNRVFQL
EPTLMVRDSVKTLSN
>Mature_334_residues
ATIKDVAKHASVSTSTVSHVLNKTRFVSEDISERVMAAVAELNYAPSALARSLKVNHTKTFGMLVTTSTNPFFGEVVKGV
ERRCYEKGYNLILCNTEGDAERVHSSLDTLLQKRVDGLLLMCTEIENQVLELFSRYQPVPTVVMDWGLIDFPSDKIQDNS
HHGGYLATKHLIEQGHTEIGCLTGPLNKLQAQQRLSGFVQAMEESGLEINKNWIASGNFECEGGEAAFNEIHAKGKLPTA
LFVCNDMMAMGMINCASKKGISVPNDISIVGYDDIKMAKYITPSLTTIHQPKHRLGQQAVDSLLEKIATKSETNRVFQLE
PTLMVRDSVKTLSN

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=54.4072948328268, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI1787948, Length=332, Percent_Identity=48.7951807228916, Blast_Score=345, Evalue=3e-96,
Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=31.0240963855422, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1789202, Length=336, Percent_Identity=35.4166666666667, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1788474, Length=335, Percent_Identity=31.6417910447761, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=29.2834890965732, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1789068, Length=307, Percent_Identity=29.9674267100977, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1786540, Length=330, Percent_Identity=30, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1787906, Length=322, Percent_Identity=24.223602484472, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1790715, Length=327, Percent_Identity=24.7706422018349, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994940, Length=334, Percent_Identity=25.1497005988024, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789456, Length=328, Percent_Identity=26.219512195122, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786268, Length=299, Percent_Identity=23.0769230769231, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790689, Length=317, Percent_Identity=23.6593059936909, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36977; Mature: 36846

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAKHASVSTSTVSHVLNKTRFVSEDISERVMAAVAELNYAPSALARSLKVNHTK
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEE
TFGMLVTTSTNPFFGEVVKGVERRCYEKGYNLILCNTEGDAERVHSSLDTLLQKRVDGLL
EEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCE
LMCTEIENQVLELFSRYQPVPTVVMDWGLIDFPSDKIQDNSHHGGYLATKHLIEQGHTEI
EHHHHHHHHHHHHHHHCCCCCHHEEECCCCCCCCHHCCCCCCCCCHHHHHHHHHHCCCCE
GCLTGPLNKLQAQQRLSGFVQAMEESGLEINKNWIASGNFECEGGEAAFNEIHAKGKLPT
EEECCCHHHHHHHHHHHHHHHHHHHCCCEECCCEEECCCEECCCCHHHHHHHHCCCCCCH
ALFVCNDMMAMGMINCASKKGISVPNDISIVGYDDIKMAKYITPSLTTIHQPKHRLGQQA
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHCCCCHHHHCCHHHHHHHHH
VDSLLEKIATKSETNRVFQLEPTLMVRDSVKTLSN
HHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHCC
>Mature Secondary Structure 
ATIKDVAKHASVSTSTVSHVLNKTRFVSEDISERVMAAVAELNYAPSALARSLKVNHTK
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEE
TFGMLVTTSTNPFFGEVVKGVERRCYEKGYNLILCNTEGDAERVHSSLDTLLQKRVDGLL
EEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCE
LMCTEIENQVLELFSRYQPVPTVVMDWGLIDFPSDKIQDNSHHGGYLATKHLIEQGHTEI
EHHHHHHHHHHHHHHHCCCCCHHEEECCCCCCCCHHCCCCCCCCCHHHHHHHHHHCCCCE
GCLTGPLNKLQAQQRLSGFVQAMEESGLEINKNWIASGNFECEGGEAAFNEIHAKGKLPT
EEECCCHHHHHHHHHHHHHHHHHHHCCCEECCCEEECCCEECCCCHHHHHHHHCCCCCCH
ALFVCNDMMAMGMINCASKKGISVPNDISIVGYDDIKMAKYITPSLTTIHQPKHRLGQQA
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHCCCCHHHHCCHHHHHHHHH
VDSLLEKIATKSETNRVFQLEPTLMVRDSVKTLSN
HHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA