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The map label for this gene is rhtB [H]

Identifier: 59711945

GI number: 59711945

Start: 1485050

End: 1485679

Strand: Reverse

Name: rhtB [H]

Synonym: VF_1338

Alternate gene names: 59711945

Gene position: 1485679-1485050 (Counterclockwise)

Preceding gene: 59711947

Following gene: 59711944

Centisome position: 51.27

GC content: 39.37

Gene sequence:

>630_bases
ATGGCGTTAGAAATATGGATTTATTATGTGTTAGCGGTCTTGGTTCTTACGGCTTCACCCGGGCCCAGTTCATTATTATG
CATGACAAAAGGGGTGACGCATGGATGGAAACACGGTTTTTTTACGGCGTTAGGTAGCTTAAGTGCCATCACTATTATCC
TGTCACTTTCTTTTACTGGGCTTGGGGTTGTTATCGCTTCATCTGAATTGGTATTTACAATCATTAAGTGGATTGGTGCG
ACATACCTCGTTTATATGGGAATAAAAGCCTTTTTATCAAAACAAGAAAGTTATACATCAACGGCTGAAAATACAGAGAT
TAAAACAGGATTTAAAGCGCATTTTGTAAGTGGTTTTATTGTTGGTGCCAGTAACCCTAAAGCGATTTTGTTTTTTACTG
CTCTGTTTCCTCAATTTATTGATCCATCTGCGGTTCTCGTTCCTCAATACCTGATATTTGCGAGTACATTTATTGTATTA
GAGCTTTCTTGGTTACTGTTTTATGCGTATCTAGGTTCTACATCGTCTCGTTGGCTAATGGTTAAAGGGCGTGCTAAATG
GTTCAACAAATTAAGCGGAACTGTTTTTGTCGGAGCAGGGGCTTTATTGTCTACCGCTAGCCGAAATTGA

Upstream 100 bases:

>100_bases
CGTTAAAGCAAGATGAGCGGGATGCGTGATTTGTAAAATTCCATATATACGCTTTAATAGATCCTTTATGGATAAAAAAA
TGGAATAAGGAGATTCTATT

Downstream 100 bases:

>100_bases
TGAGGGCTGTGTAAACTTTTAGTAAAGAGCGTAAATTAGTGATCTTTCTAGCAGAATGTGTGGTGGAAGATAGGTATAAG
AGCCTCAGTTGTTTTATGTA

Product: neutral amino-acid efflux system

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MALEIWIYYVLAVLVLTASPGPSSLLCMTKGVTHGWKHGFFTALGSLSAITIILSLSFTGLGVVIASSELVFTIIKWIGA
TYLVYMGIKAFLSKQESYTSTAENTEIKTGFKAHFVSGFIVGASNPKAILFFTALFPQFIDPSAVLVPQYLIFASTFIVL
ELSWLLFYAYLGSTSSRWLMVKGRAKWFNKLSGTVFVGAGALLSTASRN

Sequences:

>Translated_209_residues
MALEIWIYYVLAVLVLTASPGPSSLLCMTKGVTHGWKHGFFTALGSLSAITIILSLSFTGLGVVIASSELVFTIIKWIGA
TYLVYMGIKAFLSKQESYTSTAENTEIKTGFKAHFVSGFIVGASNPKAILFFTALFPQFIDPSAVLVPQYLIFASTFIVL
ELSWLLFYAYLGSTSSRWLMVKGRAKWFNKLSGTVFVGAGALLSTASRN
>Mature_208_residues
ALEIWIYYVLAVLVLTASPGPSSLLCMTKGVTHGWKHGFFTALGSLSAITIILSLSFTGLGVVIASSELVFTIIKWIGAT
YLVYMGIKAFLSKQESYTSTAENTEIKTGFKAHFVSGFIVGASNPKAILFFTALFPQFIDPSAVLVPQYLIFASTFIVLE
LSWLLFYAYLGSTSSRWLMVKGRAKWFNKLSGTVFVGAGALLSTASRN

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=208, Percent_Identity=31.25, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788099, Length=208, Percent_Identity=31.7307692307692, Blast_Score=99, Evalue=1e-22,
Organism=Escherichia coli, GI48994978, Length=189, Percent_Identity=25.9259259259259, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22739; Mature: 22608

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALEIWIYYVLAVLVLTASPGPSSLLCMTKGVTHGWKHGFFTALGSLSAITIILSLSFTG
CEEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
LGVVIASSELVFTIIKWIGATYLVYMGIKAFLSKQESYTSTAENTEIKTGFKAHFVSGFI
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHEEEEEEE
VGASNPKAILFFTALFPQFIDPSAVLVPQYLIFASTFIVLELSWLLFYAYLGSTSSRWLM
EECCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
VKGRAKWFNKLSGTVFVGAGALLSTASRN
EECHHHHHHHCCCEEEEECCHHHHCCCCC
>Mature Secondary Structure 
ALEIWIYYVLAVLVLTASPGPSSLLCMTKGVTHGWKHGFFTALGSLSAITIILSLSFTG
EEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
LGVVIASSELVFTIIKWIGATYLVYMGIKAFLSKQESYTSTAENTEIKTGFKAHFVSGFI
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHEEEEEEE
VGASNPKAILFFTALFPQFIDPSAVLVPQYLIFASTFIVLELSWLLFYAYLGSTSSRWLM
EECCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
VKGRAKWFNKLSGTVFVGAGALLSTASRN
EECHHHHHHHCCCEEEEECCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]