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The map label for this gene is lacA [H]

Identifier: 59711933

GI number: 59711933

Start: 1473671

End: 1474270

Strand: Reverse

Name: lacA [H]

Synonym: VF_1326

Alternate gene names: 59711933

Gene position: 1474270-1473671 (Counterclockwise)

Preceding gene: 59711934

Following gene: 59711932

Centisome position: 50.88

GC content: 40.5

Gene sequence:

>600_bases
ATGAGTCATAAATTGTCGGATTTTTCAGTTCAAATTCCCATAAATAAAGAACTGTGCGCATTTCAGGATGAAGTAAAAGA
GCAACTGTATGACTTTAACCACTCCCGACCATCAGAACATCAGTTAAGAGCCGATGTATTGCAATCTATCCTTGGTCAAT
ATAAGGGAGTGACTATCGTACCTCCATTTTATTGTGATATGGGAAAGAACATACATTTTGAGTGTGGTGGATTTTTAAAT
TCAGGAGTTAAGATCTTAGATCTTGCTCCTGTTTTTATTGGAGCACATGTTCAAGTTGGCCCAAATGTTGTGATCAGTAC
AGCAGGGCATCCTTTTGATTTAGCGGAGCGAGTATTACCGATTGCTTCTGCTAACCCAATTAAGATTGGAGATAACGTTT
GGATTGGTGCTAACGCTGTTATATTGGATGGTGTGACAATCGGTGCTCGAAGTGTGATTGGTGCAGGAAGTGTAGTGACA
AAAGATATTCCGCCAGACTGTGTCGCTGTCGGTAATCCTTGTCGAGTGATTAAGACGATTACCCATTCAGACATGCCATC
TGACGATGAACTTGATGAAATGTGGGCAGATCTAACATAA

Upstream 100 bases:

>100_bases
TGCGTATGTCTATAATTTAGCTTATGATAAAGCATTCCCTATTAATGAGGTAAAACAAGTAGCTTAATAAGTAGGGAGTA
AGATTAACTAAGGAGTGGTT

Downstream 100 bases:

>100_bases
TTATTATTATCAACAAAATACACAATTGAATAATTTGTGAACAATATCATTTATTAGTGATCACAACGGCACATTGTTTT
AAAAAAATTTCCTAGAATAT

Product: acetyltransferase

Products: NA

Alternate protein names: GAT; Thiogalactoside acetyltransferase [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MSHKLSDFSVQIPINKELCAFQDEVKEQLYDFNHSRPSEHQLRADVLQSILGQYKGVTIVPPFYCDMGKNIHFECGGFLN
SGVKILDLAPVFIGAHVQVGPNVVISTAGHPFDLAERVLPIASANPIKIGDNVWIGANAVILDGVTIGARSVIGAGSVVT
KDIPPDCVAVGNPCRVIKTITHSDMPSDDELDEMWADLT

Sequences:

>Translated_199_residues
MSHKLSDFSVQIPINKELCAFQDEVKEQLYDFNHSRPSEHQLRADVLQSILGQYKGVTIVPPFYCDMGKNIHFECGGFLN
SGVKILDLAPVFIGAHVQVGPNVVISTAGHPFDLAERVLPIASANPIKIGDNVWIGANAVILDGVTIGARSVIGAGSVVT
KDIPPDCVAVGNPCRVIKTITHSDMPSDDELDEMWADLT
>Mature_198_residues
SHKLSDFSVQIPINKELCAFQDEVKEQLYDFNHSRPSEHQLRADVLQSILGQYKGVTIVPPFYCDMGKNIHFECGGFLNS
GVKILDLAPVFIGAHVQVGPNVVISTAGHPFDLAERVLPIASANPIKIGDNVWIGANAVILDGVTIGARSVIGAGSVVTK
DIPPDCVAVGNPCRVIKTITHSDMPSDDELDEMWADLT

Specific function: May assist cellular detoxification by acetylating non- metabolizable pyranosides, thereby preventing their reentry into the cell [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=160, Percent_Identity=45, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1786664, Length=157, Percent_Identity=41.4012738853503, Blast_Score=124, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6322243, Length=169, Percent_Identity=44.9704142011834, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: =2.3.1.18 [H]

Molecular weight: Translated: 21504; Mature: 21373

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKLSDFSVQIPINKELCAFQDEVKEQLYDFNHSRPSEHQLRADVLQSILGQYKGVTIV
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEE
PPFYCDMGKNIHFECGGFLNSGVKILDLAPVFIGAHVQVGPNVVISTAGHPFDLAERVLP
CCHHHCCCCCEEEEECCCCCCCEEEEEECHHHEECEEEECCCEEEEECCCCHHHHHHHHC
IASANPIKIGDNVWIGANAVILDGVTIGARSVIGAGSVVTKDIPPDCVAVGNPCRVIKTI
CCCCCCEEECCEEEECCCEEEEECEEECCHHHCCCCCEEECCCCCCCEECCCCHHEEEEH
THSDMPSDDELDEMWADLT
HCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
SHKLSDFSVQIPINKELCAFQDEVKEQLYDFNHSRPSEHQLRADVLQSILGQYKGVTIV
CCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEE
PPFYCDMGKNIHFECGGFLNSGVKILDLAPVFIGAHVQVGPNVVISTAGHPFDLAERVLP
CCHHHCCCCCEEEEECCCCCCCEEEEEECHHHEECEEEECCCEEEEECCCCHHHHHHHHC
IASANPIKIGDNVWIGANAVILDGVTIGARSVIGAGSVVTKDIPPDCVAVGNPCRVIKTI
CCCCCCEEECCEEEECCCEEEEECEEECCHHHCCCCCEEECCCCCCCEECCCCHHEEEEH
THSDMPSDDELDEMWADLT
HCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3922433; 3901000; 9278503; 6444453; 11937062 [H]