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The map label for this gene is ttcA [H]

Identifier: 59711918

GI number: 59711918

Start: 1459898

End: 1460821

Strand: Direct

Name: ttcA [H]

Synonym: VF_1311

Alternate gene names: 59711918

Gene position: 1459898-1460821 (Clockwise)

Preceding gene: 59711917

Following gene: 59711922

Centisome position: 50.38

GC content: 37.77

Gene sequence:

>924_bases
ATGAGCGAATTAACTAAAGCGCAGCAATACAATTTCAATAAGCTTCAAAAGAAAATCCGTCGTAATACTGGTAATGCTAT
TGCCGATTACAATATGATTGAAGACGGTGACCGCATCATGGTATGTCTATCTGGTGGTAAAGATAGCTTCACTATGCTTG
ATATCTTAATGAGCTTAAAGAAAAGTGCGCCGATCTCATTTGACTTAATCGCTGTAAACCTAGACCAGAAACAACCAGGT
TTCCCTGCACATATTCTTCCTGAATACCTTGAGAAGTTAGGTGTAGAATACAAAATTGTAGAAGAAGATACCTATTCTAT
CGTTCAGGATAAAATTCCTGAAGGTAAAACAACTTGTTCTCTATGTTCACGTTTACGTCGTGGCATTCTTTACCGCACAG
CAAAAGAACTAGGTGCAACTAAAATTGCTCTTGGTCACCATCGTGATGATATTCTTGAAACTATGTTCTTAAACATGTTC
TACGGCGGTAAATTAAAAGGCATGCCACCAAAACTAGTTTCAGACAATGGTGAGCACGTTGTAATCCGTCCTTTAGCGTA
CTGTCGTGAAAAAGACATTATCAAATACGCTGACATGCGTGATTACCCTATCATCCCATGTAATCTTTGTGGTTCACAAC
CTAATATGCAACGTCAAAACGTTAAGCAAATGCTTAATACATGGGATAAGCAGTTTCCTGGACGTATTGAGACTATGTTT
ACTGCAATGCAAAATGTAGTTCCAAGTCATTTAGCTGACTTTAATACGTTTGACTTTAAGAGCATTAACCGTGATTCAGG
TGTTATTAACGGTGGTGATATTGGTTTTGATAAAGAAGAAATGCCAACATTACCCGTTGATGCTGACGATGCAGTTGCAG
AGTTTGACCCATCATTAAAATTAGATGTTGTGAATGTAGATTAA

Upstream 100 bases:

>100_bases
GCCCTTTTCTCCCAAAATAATCCCGTCTAAACTCGCTTATTGATCTTGTTTGGGTATAATACCCGCCTTATCAGTTCCAC
CATTAGTTTAGAGTAAGTAA

Downstream 100 bases:

>100_bases
TTATCTAATACCAAATAAAAAGGCGCAAACGTTAACTGTTTGCGCCTTTTTTTATTTTGATTTTATTCGAACTATTTTAC
TAGCCATGGAGTAACTTTTA

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSELTKAQQYNFNKLQKKIRRNTGNAIADYNMIEDGDRIMVCLSGGKDSFTMLDILMSLKKSAPISFDLIAVNLDQKQPG
FPAHILPEYLEKLGVEYKIVEEDTYSIVQDKIPEGKTTCSLCSRLRRGILYRTAKELGATKIALGHHRDDILETMFLNMF
YGGKLKGMPPKLVSDNGEHVVIRPLAYCREKDIIKYADMRDYPIIPCNLCGSQPNMQRQNVKQMLNTWDKQFPGRIETMF
TAMQNVVPSHLADFNTFDFKSINRDSGVINGGDIGFDKEEMPTLPVDADDAVAEFDPSLKLDVVNVD

Sequences:

>Translated_307_residues
MSELTKAQQYNFNKLQKKIRRNTGNAIADYNMIEDGDRIMVCLSGGKDSFTMLDILMSLKKSAPISFDLIAVNLDQKQPG
FPAHILPEYLEKLGVEYKIVEEDTYSIVQDKIPEGKTTCSLCSRLRRGILYRTAKELGATKIALGHHRDDILETMFLNMF
YGGKLKGMPPKLVSDNGEHVVIRPLAYCREKDIIKYADMRDYPIIPCNLCGSQPNMQRQNVKQMLNTWDKQFPGRIETMF
TAMQNVVPSHLADFNTFDFKSINRDSGVINGGDIGFDKEEMPTLPVDADDAVAEFDPSLKLDVVNVD
>Mature_306_residues
SELTKAQQYNFNKLQKKIRRNTGNAIADYNMIEDGDRIMVCLSGGKDSFTMLDILMSLKKSAPISFDLIAVNLDQKQPGF
PAHILPEYLEKLGVEYKIVEEDTYSIVQDKIPEGKTTCSLCSRLRRGILYRTAKELGATKIALGHHRDDILETMFLNMFY
GGKLKGMPPKLVSDNGEHVVIRPLAYCREKDIIKYADMRDYPIIPCNLCGSQPNMQRQNVKQMLNTWDKQFPGRIETMFT
AMQNVVPSHLADFNTFDFKSINRDSGVINGGDIGFDKEEMPTLPVDADDAVAEFDPSLKLDVVNVD

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=307, Percent_Identity=72.6384364820847, Blast_Score=482, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI193211090, Length=245, Percent_Identity=31.4285714285714, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17540090, Length=231, Percent_Identity=27.7056277056277, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24651972, Length=223, Percent_Identity=27.8026905829596, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 34701; Mature: 34570

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELTKAQQYNFNKLQKKIRRNTGNAIADYNMIEDGDRIMVCLSGGKDSFTMLDILMSLK
CCCHHHHHHCCHHHHHHHHHHCCCCEEECCCEECCCCEEEEEEECCCCHHHHHHHHHHHH
KSAPISFDLIAVNLDQKQPGFPAHILPEYLEKLGVEYKIVEEDTYSIVQDKIPEGKTTCS
HCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEECCHHHHHHHHCCCCCCHHHH
LCSRLRRGILYRTAKELGATKIALGHHRDDILETMFLNMFYGGKLKGMPPKLVSDNGEHV
HHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEE
VIRPLAYCREKDIIKYADMRDYPIIPCNLCGSQPNMQRQNVKQMLNTWDKQFPGRIETMF
EECCHHHHCCCCCEECCCCCCCCEEEHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
TAMQNVVPSHLADFNTFDFKSINRDSGVINGGDIGFDKEEMPTLPVDADDAVAEFDPSLK
HHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCHHHCCCCCCCCCCHHHHCCCCCE
LDVVNVD
EEEEECC
>Mature Secondary Structure 
SELTKAQQYNFNKLQKKIRRNTGNAIADYNMIEDGDRIMVCLSGGKDSFTMLDILMSLK
CCHHHHHHCCHHHHHHHHHHCCCCEEECCCEECCCCEEEEEEECCCCHHHHHHHHHHHH
KSAPISFDLIAVNLDQKQPGFPAHILPEYLEKLGVEYKIVEEDTYSIVQDKIPEGKTTCS
HCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEECCHHHHHHHHCCCCCCHHHH
LCSRLRRGILYRTAKELGATKIALGHHRDDILETMFLNMFYGGKLKGMPPKLVSDNGEHV
HHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCEE
VIRPLAYCREKDIIKYADMRDYPIIPCNLCGSQPNMQRQNVKQMLNTWDKQFPGRIETMF
EECCHHHHCCCCCEECCCCCCCCEEEHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
TAMQNVVPSHLADFNTFDFKSINRDSGVINGGDIGFDKEEMPTLPVDADDAVAEFDPSLK
HHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCHHHCCCCCCCCCCHHHHCCCCCE
LDVVNVD
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA