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The map label for this gene is pheT

Identifier: 59711843

GI number: 59711843

Start: 1373288

End: 1375675

Strand: Direct

Name: pheT

Synonym: VF_1236

Alternate gene names: 59711843

Gene position: 1373288-1375675 (Clockwise)

Preceding gene: 59711842

Following gene: 59711844

Centisome position: 47.4

GC content: 44.26

Gene sequence:

>2388_bases
ATGAAATTCAGTGAATCTTGGCTACGCGAGTGGGTTAAACCTGCTATTAACAGCGAAGAGCTAGCTCACCAAATCACAAT
GGCTGGTTTGGAAGTTGATGAAGTTGCACCTGTTGCTGGTGAGTTCACCGGCGTTAAAGTAGGTAAAGTGGTTGAGTGTG
GTCAACACCCAGACGCAGACAAACTTCGTGTAACTAAAATTGATATCGGCGAAGAAGAGCTTTTAGACATCGTTTGTGGT
GCATCTAACTGTCGTCTTGGACTAACGGTTGCAGTTGCGACTGTTGGCGCAGTACTTCCTGGTAACTTCAAAATTAAGAA
AGCAAAACTACGTGGTGTTCCATCACACGGTATGCTTTGTTCTTTCTCTGAGCTAGGTATCGATGTTGAATCTGAAGGTA
TTTTAGAATTACCTGAAGGTACAACACTAGGTGCAGATGTTCGTGATATTTTAGAACTTAACGACGTTGCTATCGACGTT
GACTTAACAGCAAACCGTGCTGACTGTTTCAGCATTCGTGGTTTAGCTCGTGAAGTAGGAGTTCTTAACCGTGCAGACGT
TACAGAACCTACTATTGCGGCAGTAGCGACAAGCATTGAAGATGCAGTATCTATTGAAGTTAAAGCAACTGAAGCGTGTC
CACGTTACCTTGGTCGTGTTATCAAGAACGTAAACGCTAAAGCTGAAACACCAATTTGGATGCAAGAGAAACTGCGTCGT
TGTGGTATTCGCTCAATCGATGCGATTGTTGATATTACAAACTACGTAATGCTTGAGCAAGGCCAACCTATGCACGCGTT
TGATCTAGCTAAGATCGACGGTGGCATTGTGGTTCGTATGGCTGAGCAAGATGAGAAACTGACTCTTCTTGATGGCAACG
AAGCAAAATTAAACGACAACACATTAGTGATTGCAGACCAAAATAAAGCACTAGCAATCGCTGGTATCTTTGGTGGTCAA
GAGTCTGGTGTTACTTCTGAAACAACTGACATCATGCTTGAGTGTGCATTCTTCGCACCTGATCACATCCGTGGTCGTGC
ACGTGCATACGGTCTACACACTGATTCTTCTCTACGTTTTGAACGTGGTGTTGATTCAACGCTACAAGCAGCAGCAATGG
AGCGCGCAACACAGCTTCTAGTTGAACTTTGTGGTGGTGAAGTAGCTCCTGTTGTTGCTGTTGAATCAGAAGCTGATTTA
CCAAAAGCAAACACAGTTGAATTACGTCGTAGTAAGTTAGATGGTCTATTAGGTCATCACATCCCATCAACAGATGTGGT
TGAGATCCTTACTCGTCTAGGTTGTGCAGTTGAAAGTACTGACACAGGTTGGACTGCAAGCTCTCCATCATGGCGTTTTG
ATATTGCAATCGAGCAAGACCTAATTGAAGAAGTAGGTCGTATCTACGGTTACAATAACATTCCTAACCAAGCGCCTGTT
GCTGCACTTAACATGAACGATCACAAAGAAGCAAACCAACCGCTTAAGCGTGTTCGTGATCTTCTTGTTGACCGTGGTTA
CCACGAAGCAATTACCTACAGCTTTGTAGAGCCAGAGCAACAAAAACTGATCGTGCCTGAGATTGAGCCGTTAATTCTGC
CATTCCCAATCTCAGCTGAAATGTCAGCAATGCGTTTAAGCCTATGGACTGGTTTGATCAATACCGTTGTTCATAACCAA
AAACGTCAACAACCACGCGTTCGTTTATTCGAATCTGGTCTGCGTTTTATTCCAGAAGAATCAGCAGAAAACGGCATGCG
TCAAGAAATGATGCTTGCTGGTATCATCGCTGGTACTCGTGGTGAAGAACACTGGGATATCGCAACAAACACAGTTGATT
TCTTTGATCTTAAAGGCGATTTAGAAGCGGTTCTTGAACTAACTGCTAATGAATTAGCGTATGAGTTTAAAGCGGCTAAG
CACCCAGCACTTCACCCAGGCCAAACAGCAGCTATCGTTGTTGATGGTAAAGAAGTTGGTTTCATTGGTACAGTACACCC
AGAGCTAGAGCGTAAGTTTGGCTTAAACGGTCGTACAATCATGTTTGAAATCGAGTGGGACGCAATCAATACTCGCGTTA
TTCCAGAAGCTGCATCAGTATCTAAGTTCCCTGCAAACCGTCGTGATATCGCGATCGTTGCAGACGAAGCAATTGCTTCT
GGTGACATCGTTGCTGAATGTCTAGCATCAGGTGGCGAGCTATTAACTAGCGCGAAACTGTTCGATGTTTACCGTGGTCA
AGGCGTTGAAGAAGGTAAGAAGAGTCTTGCAATCGCACTGACACTTCAATCTGTTGAGCGTACGCTAGAAGAGGCTGATA
TCACTTCAGCTGTTGATGCTATCGTTGCTGCAATTGGTGAGAAGTTCTCAGCAACACTGCGTGACTAA

Upstream 100 bases:

>100_bases
GTCTAACTATGCTTCGTTACGGCGTAAATGACCTTCGTGCGTTCTTCGAGAACGATCTTCGTTTCCTTAAACAATTCAAG
TAATTCGGGGCTAGAAACTT

Downstream 100 bases:

>100_bases
TATACTTCTTTTTTAACGAAAGAATATAAGATCCAGTACTGACATTTGTTGGTACTGGATTTTTTTTATTCAAAATTTAA
GATTTATAAATTAATCTTGA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 795; Mature: 795

Protein sequence:

>795_residues
MKFSESWLREWVKPAINSEELAHQITMAGLEVDEVAPVAGEFTGVKVGKVVECGQHPDADKLRVTKIDIGEEELLDIVCG
ASNCRLGLTVAVATVGAVLPGNFKIKKAKLRGVPSHGMLCSFSELGIDVESEGILELPEGTTLGADVRDILELNDVAIDV
DLTANRADCFSIRGLAREVGVLNRADVTEPTIAAVATSIEDAVSIEVKATEACPRYLGRVIKNVNAKAETPIWMQEKLRR
CGIRSIDAIVDITNYVMLEQGQPMHAFDLAKIDGGIVVRMAEQDEKLTLLDGNEAKLNDNTLVIADQNKALAIAGIFGGQ
ESGVTSETTDIMLECAFFAPDHIRGRARAYGLHTDSSLRFERGVDSTLQAAAMERATQLLVELCGGEVAPVVAVESEADL
PKANTVELRRSKLDGLLGHHIPSTDVVEILTRLGCAVESTDTGWTASSPSWRFDIAIEQDLIEEVGRIYGYNNIPNQAPV
AALNMNDHKEANQPLKRVRDLLVDRGYHEAITYSFVEPEQQKLIVPEIEPLILPFPISAEMSAMRLSLWTGLINTVVHNQ
KRQQPRVRLFESGLRFIPEESAENGMRQEMMLAGIIAGTRGEEHWDIATNTVDFFDLKGDLEAVLELTANELAYEFKAAK
HPALHPGQTAAIVVDGKEVGFIGTVHPELERKFGLNGRTIMFEIEWDAINTRVIPEAASVSKFPANRRDIAIVADEAIAS
GDIVAECLASGGELLTSAKLFDVYRGQGVEEGKKSLAIALTLQSVERTLEEADITSAVDAIVAAIGEKFSATLRD

Sequences:

>Translated_795_residues
MKFSESWLREWVKPAINSEELAHQITMAGLEVDEVAPVAGEFTGVKVGKVVECGQHPDADKLRVTKIDIGEEELLDIVCG
ASNCRLGLTVAVATVGAVLPGNFKIKKAKLRGVPSHGMLCSFSELGIDVESEGILELPEGTTLGADVRDILELNDVAIDV
DLTANRADCFSIRGLAREVGVLNRADVTEPTIAAVATSIEDAVSIEVKATEACPRYLGRVIKNVNAKAETPIWMQEKLRR
CGIRSIDAIVDITNYVMLEQGQPMHAFDLAKIDGGIVVRMAEQDEKLTLLDGNEAKLNDNTLVIADQNKALAIAGIFGGQ
ESGVTSETTDIMLECAFFAPDHIRGRARAYGLHTDSSLRFERGVDSTLQAAAMERATQLLVELCGGEVAPVVAVESEADL
PKANTVELRRSKLDGLLGHHIPSTDVVEILTRLGCAVESTDTGWTASSPSWRFDIAIEQDLIEEVGRIYGYNNIPNQAPV
AALNMNDHKEANQPLKRVRDLLVDRGYHEAITYSFVEPEQQKLIVPEIEPLILPFPISAEMSAMRLSLWTGLINTVVHNQ
KRQQPRVRLFESGLRFIPEESAENGMRQEMMLAGIIAGTRGEEHWDIATNTVDFFDLKGDLEAVLELTANELAYEFKAAK
HPALHPGQTAAIVVDGKEVGFIGTVHPELERKFGLNGRTIMFEIEWDAINTRVIPEAASVSKFPANRRDIAIVADEAIAS
GDIVAECLASGGELLTSAKLFDVYRGQGVEEGKKSLAIALTLQSVERTLEEADITSAVDAIVAAIGEKFSATLRD
>Mature_795_residues
MKFSESWLREWVKPAINSEELAHQITMAGLEVDEVAPVAGEFTGVKVGKVVECGQHPDADKLRVTKIDIGEEELLDIVCG
ASNCRLGLTVAVATVGAVLPGNFKIKKAKLRGVPSHGMLCSFSELGIDVESEGILELPEGTTLGADVRDILELNDVAIDV
DLTANRADCFSIRGLAREVGVLNRADVTEPTIAAVATSIEDAVSIEVKATEACPRYLGRVIKNVNAKAETPIWMQEKLRR
CGIRSIDAIVDITNYVMLEQGQPMHAFDLAKIDGGIVVRMAEQDEKLTLLDGNEAKLNDNTLVIADQNKALAIAGIFGGQ
ESGVTSETTDIMLECAFFAPDHIRGRARAYGLHTDSSLRFERGVDSTLQAAAMERATQLLVELCGGEVAPVVAVESEADL
PKANTVELRRSKLDGLLGHHIPSTDVVEILTRLGCAVESTDTGWTASSPSWRFDIAIEQDLIEEVGRIYGYNNIPNQAPV
AALNMNDHKEANQPLKRVRDLLVDRGYHEAITYSFVEPEQQKLIVPEIEPLILPFPISAEMSAMRLSLWTGLINTVVHNQ
KRQQPRVRLFESGLRFIPEESAENGMRQEMMLAGIIAGTRGEEHWDIATNTVDFFDLKGDLEAVLELTANELAYEFKAAK
HPALHPGQTAAIVVDGKEVGFIGTVHPELERKFGLNGRTIMFEIEWDAINTRVIPEAASVSKFPANRRDIAIVADEAIAS
GDIVAECLASGGELLTSAKLFDVYRGQGVEEGKKSLAIALTLQSVERTLEEADITSAVDAIVAAIGEKFSATLRD

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI124028525, Length=563, Percent_Identity=23.4458259325044, Blast_Score=82, Evalue=3e-15,
Organism=Escherichia coli, GI1788006, Length=795, Percent_Identity=62.2641509433962, Blast_Score=1025, Evalue=0.0,
Organism=Drosophila melanogaster, GI21355211, Length=555, Percent_Identity=22.8828828828829, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_VIBF1 (Q5E5G5)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_204619.1
- ProteinModelPortal:   Q5E5G5
- SMR:   Q5E5G5
- STRING:   Q5E5G5
- GeneID:   3278574
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1236
- NMPDR:   fig|312309.3.peg.1085
- eggNOG:   COG0072
- HOGENOM:   HBG399638
- OMA:   ADKLRVC
- PhylomeDB:   Q5E5G5
- ProtClustDB:   PRK00629
- BioCyc:   VFIS312309:VF1236-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00283
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547
- Gene3D:   G3DSA:3.50.40.10
- Gene3D:   G3DSA:3.30.56.20
- Gene3D:   G3DSA:3.30.70.380
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00873
- SMART:   SM00874
- SMART:   SM00896
- TIGRFAMs:   TIGR00472

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind; SSF56037 B3_4; SSF54991 Fdx_AntiC_bd; SSF50249 Nucleic_acid_OB; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 86438; Mature: 86438

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS51483 B5; PS51447 FDX_ACB; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSESWLREWVKPAINSEELAHQITMAGLEVDEVAPVAGEFTGVKVGKVVECGQHPDAD
CCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCEEECCEEECCCCCCCC
KLRVTKIDIGEEELLDIVCGASNCRLGLTVAVATVGAVLPGNFKIKKAKLRGVPSHGMLC
CEEEEEECCCHHHHHHHHCCCCCCCEEHEEHHHHHHHCCCCCEEEEEEHCCCCCCCCCEE
SFSELGIDVESEGILELPEGTTLGADVRDILELNDVAIDVDLTANRADCFSIRGLAREVG
EHHHHCCEECCCCCEECCCCCCCCCCHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHC
VLNRADVTEPTIAAVATSIEDAVSIEVKATEACPRYLGRVIKNVNAKAETPIWMQEKLRR
CCCCCCCCCCHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
CGIRSIDAIVDITNYVMLEQGQPMHAFDLAKIDGGIVVRMAEQDEKLTLLDGNEAKLNDN
CCCCHHHHHHHHHHEEEECCCCCCCCEEHEECCCCEEEEEECCCCEEEEEECCCCEECCC
TLVIADQNKALAIAGIFGGQESGVTSETTDIMLECAFFAPDHIRGRARAYGLHTDSSLRF
EEEEEECCCEEEEEEEECCCCCCCCCCCHHEEEEEEECCCHHHCCCCEEEEECCCCCCCH
ERGVDSTLQAAAMERATQLLVELCGGEVAPVVAVESEADLPKANTVELRRSKLDGLLGHH
HCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
IPSTDVVEILTRLGCAVESTDTGWTASSPSWRFDIAIEQDLIEEVGRIYGYNNIPNQAPV
CCCHHHHHHHHHHCCEEECCCCCCCCCCCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCE
AALNMNDHKEANQPLKRVRDLLVDRGYHEAITYSFVEPEQQKLIVPEIEPLILPFPISAE
EEECCCCCHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCEEECCCCCCEEEECCCCCC
MSAMRLSLWTGLINTVVHNQKRQQPRVRLFESGLRFIPEESAENGMRQEMMLAGIIAGTR
HHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHCCCC
GEEHWDIATNTVDFFDLKGDLEAVLELTANELAYEFKAAKHPALHPGQTAAIVVDGKEVG
CCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
FIGTVHPELERKFGLNGRTIMFEIEWDAINTRVIPEAASVSKFPANRRDIAIVADEAIAS
EEECCCHHHHHHHCCCCCEEEEEEEECCCCCEECCCCHHHCCCCCCCCCEEEEECCCCCC
GDIVAECLASGGELLTSAKLFDVYRGQGVEEGKKSLAIALTLQSVERTLEEADITSAVDA
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHH
IVAAIGEKFSATLRD
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFSESWLREWVKPAINSEELAHQITMAGLEVDEVAPVAGEFTGVKVGKVVECGQHPDAD
CCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCEEECCEEECCCCCCCC
KLRVTKIDIGEEELLDIVCGASNCRLGLTVAVATVGAVLPGNFKIKKAKLRGVPSHGMLC
CEEEEEECCCHHHHHHHHCCCCCCCEEHEEHHHHHHHCCCCCEEEEEEHCCCCCCCCCEE
SFSELGIDVESEGILELPEGTTLGADVRDILELNDVAIDVDLTANRADCFSIRGLAREVG
EHHHHCCEECCCCCEECCCCCCCCCCHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHC
VLNRADVTEPTIAAVATSIEDAVSIEVKATEACPRYLGRVIKNVNAKAETPIWMQEKLRR
CCCCCCCCCCHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
CGIRSIDAIVDITNYVMLEQGQPMHAFDLAKIDGGIVVRMAEQDEKLTLLDGNEAKLNDN
CCCCHHHHHHHHHHEEEECCCCCCCCEEHEECCCCEEEEEECCCCEEEEEECCCCEECCC
TLVIADQNKALAIAGIFGGQESGVTSETTDIMLECAFFAPDHIRGRARAYGLHTDSSLRF
EEEEEECCCEEEEEEEECCCCCCCCCCCHHEEEEEEECCCHHHCCCCEEEEECCCCCCCH
ERGVDSTLQAAAMERATQLLVELCGGEVAPVVAVESEADLPKANTVELRRSKLDGLLGHH
HCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
IPSTDVVEILTRLGCAVESTDTGWTASSPSWRFDIAIEQDLIEEVGRIYGYNNIPNQAPV
CCCHHHHHHHHHHCCEEECCCCCCCCCCCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCE
AALNMNDHKEANQPLKRVRDLLVDRGYHEAITYSFVEPEQQKLIVPEIEPLILPFPISAE
EEECCCCCHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCEEECCCCCCEEEECCCCCC
MSAMRLSLWTGLINTVVHNQKRQQPRVRLFESGLRFIPEESAENGMRQEMMLAGIIAGTR
HHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHCCCC
GEEHWDIATNTVDFFDLKGDLEAVLELTANELAYEFKAAKHPALHPGQTAAIVVDGKEVG
CCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
FIGTVHPELERKFGLNGRTIMFEIEWDAINTRVIPEAASVSKFPANRRDIAIVADEAIAS
EEECCCHHHHHHHCCCCCEEEEEEEECCCCCEECCCCHHHCCCCCCCCCEEEEECCCCCC
GDIVAECLASGGELLTSAKLFDVYRGQGVEEGKKSLAIALTLQSVERTLEEADITSAVDA
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHH
IVAAIGEKFSATLRD
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA