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The map label for this gene is pheS [H]

Identifier: 59711842

GI number: 59711842

Start: 1372287

End: 1373270

Strand: Direct

Name: pheS [H]

Synonym: VF_1235

Alternate gene names: 59711842

Gene position: 1372287-1373270 (Clockwise)

Preceding gene: 59711840

Following gene: 59711843

Centisome position: 47.36

GC content: 44.0

Gene sequence:

>984_bases
ATGCAACATCTAGACGAGATCATTGCCAATGCAACGGCAGAAATTGAACAAGCTGGCTCTTTAGTCGCTCTGGACGAAGT
CCGTGTTCAATATTTAGGTAAAAAAGGCCACTTAACACTTCAACTTCAAGGTCTAGGTAAACTTGACCCATCTGAGCGTC
GTGAAGCGGGTCAATTAATTAACAAAGGTAAGCAAGCAGTACAAGCAATGCTAACAGAGCGTAAAGACGCACTGCAAACG
GCTGAGCTTGAAGCAAAACTTGCTGCTGAAACTATCGATGTGAGCCTTCCTGGCCGTCGTATCGAGAACGGTGGTTTACA
CCCTGTTACTCGTACTGTTGAGCGTATTGAGCAATTTTTTGGCGAGCTAGGCTTTAGCACTGAGTCTGGTCCTGAAATCG
AAGATGCATTCCATAACTTCGACGCATTAAACATTGCAGAAGATCACCCAGCTCGTACTGACCACGACACCTTTTTCTTT
AACCCTGATCTAATGCTACGTACGCACACATCAGGTGTTCAGATCCGTACAATGGAAAACGGTAAACCACCATTCCGTTT
CATTGCACCTGGCCGTGTATACCGTAACGATTACGACCAAACTCACACGCCAATGTTCCACCAAGTGGAAGGTATGTTAG
TTGATGAGAACGTAAACTTTGCACAACTTAAAGGTATTCTTCACGATTTCCTTTGTAATTTCTTTGAAGAAGAAGTTGAA
GTGCGTTTCCGTCCTTCATTCTTCCCATTCACTGAGCCTTCAGCTGAAGTTGATGTTAAGCGTAAAGATGGTAAATGGTT
AGAAGTATTAGGTTGTGGCATGGTTCACCCTAATGTACTTCGCTCTGTTGGCATCGACCCTGAAAAATACTCAGGCTTTG
CGTTTGGTATGGGTGTTGAGCGTCTAACTATGCTTCGTTACGGCGTAAATGACCTTCGTGCGTTCTTCGAGAACGATCTT
CGTTTCCTTAAACAATTCAAGTAA

Upstream 100 bases:

>100_bases
TATCTAATTCAATTTGGATTTAGCGCTGTGATTTATTACTATGTGTTGTTATATGTATTTTACTCGCAACGGATACCACC
TATAAGTGGATGAGGAAACG

Downstream 100 bases:

>100_bases
TTCGGGGCTAGAAACTTATGAAATTCAGTGAATCTTGGCTACGCGAGTGGGTTAAACCTGCTATTAACAGCGAAGAGCTA
GCTCACCAAATCACAATGGC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MQHLDEIIANATAEIEQAGSLVALDEVRVQYLGKKGHLTLQLQGLGKLDPSERREAGQLINKGKQAVQAMLTERKDALQT
AELEAKLAAETIDVSLPGRRIENGGLHPVTRTVERIEQFFGELGFSTESGPEIEDAFHNFDALNIAEDHPARTDHDTFFF
NPDLMLRTHTSGVQIRTMENGKPPFRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILHDFLCNFFEEEVE
VRFRPSFFPFTEPSAEVDVKRKDGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLTMLRYGVNDLRAFFENDL
RFLKQFK

Sequences:

>Translated_327_residues
MQHLDEIIANATAEIEQAGSLVALDEVRVQYLGKKGHLTLQLQGLGKLDPSERREAGQLINKGKQAVQAMLTERKDALQT
AELEAKLAAETIDVSLPGRRIENGGLHPVTRTVERIEQFFGELGFSTESGPEIEDAFHNFDALNIAEDHPARTDHDTFFF
NPDLMLRTHTSGVQIRTMENGKPPFRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILHDFLCNFFEEEVE
VRFRPSFFPFTEPSAEVDVKRKDGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLTMLRYGVNDLRAFFENDL
RFLKQFK
>Mature_327_residues
MQHLDEIIANATAEIEQAGSLVALDEVRVQYLGKKGHLTLQLQGLGKLDPSERREAGQLINKGKQAVQAMLTERKDALQT
AELEAKLAAETIDVSLPGRRIENGGLHPVTRTVERIEQFFGELGFSTESGPEIEDAFHNFDALNIAEDHPARTDHDTFFF
NPDLMLRTHTSGVQIRTMENGKPPFRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILHDFLCNFFEEEVE
VRFRPSFFPFTEPSAEVDVKRKDGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLTMLRYGVNDLRAFFENDL
RFLKQFK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=274, Percent_Identity=33.5766423357664, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI4758340, Length=259, Percent_Identity=32.8185328185328, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1788007, Length=327, Percent_Identity=72.782874617737, Blast_Score=508, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI32566635, Length=283, Percent_Identity=33.9222614840989, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI32563657, Length=260, Percent_Identity=31.9230769230769, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17508957, Length=260, Percent_Identity=31.9230769230769, Blast_Score=131, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6321087, Length=268, Percent_Identity=32.089552238806, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=271, Percent_Identity=27.3062730627306, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI18858079, Length=258, Percent_Identity=31.7829457364341, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI17137424, Length=279, Percent_Identity=29.0322580645161, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 37145; Mature: 37145

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHLDEIIANATAEIEQAGSLVALDEVRVQYLGKKGHLTLQLQGLGKLDPSERREAGQLI
CCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHH
NKGKQAVQAMLTERKDALQTAELEAKLAAETIDVSLPGRRIENGGLHPVTRTVERIEQFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GELGFSTESGPEIEDAFHNFDALNIAEDHPARTDHDTFFFNPDLMLRTHTSGVQIRTMEN
HHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCEEEEEECC
GKPPFRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILHDFLCNFFEEEVE
CCCCEEEECCCHHHCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
VRFRPSFFPFTEPSAEVDVKRKDGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVE
EEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCHHHCCCEEECCCHH
RLTMLRYGVNDLRAFFENDLRFLKQFK
HHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQHLDEIIANATAEIEQAGSLVALDEVRVQYLGKKGHLTLQLQGLGKLDPSERREAGQLI
CCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHH
NKGKQAVQAMLTERKDALQTAELEAKLAAETIDVSLPGRRIENGGLHPVTRTVERIEQFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GELGFSTESGPEIEDAFHNFDALNIAEDHPARTDHDTFFFNPDLMLRTHTSGVQIRTMEN
HHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCEEEEEECC
GKPPFRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILHDFLCNFFEEEVE
CCCCEEEECCCHHHCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
VRFRPSFFPFTEPSAEVDVKRKDGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVE
EEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCHHHCCCEEECCCHH
RLTMLRYGVNDLRAFFENDLRFLKQFK
HHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA