Click here to switch to the map view.

The map label for this gene is nrdA [H]

Identifier: 59711809

GI number: 59711809

Start: 1336199

End: 1338475

Strand: Reverse

Name: nrdA [H]

Synonym: VF_1202

Alternate gene names: 59711809

Gene position: 1338475-1336199 (Counterclockwise)

Preceding gene: 59711810

Following gene: 59711808

Centisome position: 46.19

GC content: 39.92

Gene sequence:

>2277_bases
ATGACTCAACAGCTTACCGTTACTAAGCGAAATGGCAGCACAGAAAAAATTAATCTAGACAAGATCCATAAAGTGATCAC
ATGGGCTGCAGAAGGTCTAGATAATGTTTCTGTATCACAAGTCGAAATGAAATCTCATATCCAATTTTACGATGGCATGA
AGACTTCTGATATTCATGAAACCATCATCAAAGCTGCCGCTGATTTAATTTCTGAAGACACTCCAGATTATCAATATTTA
GCTGCTCGCTTGTCTGTTTTCCACCTTCGTAAGAAAGCATATGGCCAATTTGAGCCACCAACGCTGTTTAACCACGTTCT
TAATCTTATTGAGCTTGGTAAATACGATAAACACATCGTTGAAGACTACACTCAAGAAGAATTTGCTCTAATGGATCAAA
TGATCGATCACAATCGTGACATGGACTTCTCTTATGCTGCAGTTAAACAACTTGAAGGTAAGTACTTTGTTCAAAACCGC
GTAACGGGCGCAATTTACGAGAGTGCACAGTTCCTTTATATCCTTGTTGCGGCATGTCTATTTGCTAAATATCCAAAAGA
TACTCGTCTTGATTACATCAAGCGTTTCTATGATGCTACGTCACAATTTAAGATCTCATTGCCAACACCAATTATGTCTG
GTGTACGTACTCCTACTCGTCAATTTAGCTCATGTGTATTAATTGAGTGTGACGATAGCTTAGATTCAATTAACGCAACA
GCAGGTTCTATTGTTCGTTATGTATCTCAACGTGCAGGTATCGGTATCAACGCAGGTCGTATTCGTGCTCTTGGTTCTGA
AATCCGTGATGGTGAAGCATTCCACACAGGTTGTATCCCATTCTACAAATACTTCCAGACAGCTGTTAAATGTTGTTCAC
AAGGTGGTGTTCGTGGTGGCGCAGCAACGCTGTTCTACCCTATTTGGCACCGTGAAGTGGAATCTCTACTTGTTCTTAAA
AACAACCGTGGTGTTGAAGAGAACCGTGTTCGTCATATGGATTACGGTGTACAAATCAATAAACTGATGTACACACGCTT
AATCAAAGGCGGAAACATTAGCTTGTTCTCACCTTCAGATGTTCCAGGGCTATACGATGCATTCTTTGCTGATCAAGCTG
AGTTTGAACGCCTATATGAAAAATACGAGCAAGACACGTCGATTAAACGTGAAACAGTAAAAGCAATTGAGCTGTTCTCA
CTACTAATGCAAGAACGTGCATCAACTGGCCGTATTTACATTCAAAACGTTGACCACTGTAATACACACAGTCCATTTGA
CCCAAGTGTTGCTCCTGTACGCCAATCTAACCTATGTTTAGAAATTGCACTTCCAACTAAGCCACTAACTCACGTTAATG
ACGAAGAAGGTGAAATTGCATTATGTACGCTTTCTGCATTTAACTTAGGTGCAATTGAAAAACTAGAAGAACTTGAAGAG
CTTGCTGAGCTTGCTGTTCGTGCTCTTGATGCGCTTTTAGATTATCAAGACTACCCTCTTCCAGCAGCATACAAATCAAC
AATGAACCGCCGTACATTGGGTGTGGGTGTTATTAACTACGCTTACTACCTTGCAAAAAATGGTGTGAAATACTCTGATG
GTAGTGCAAATGGTTTAACGCACAAAACATTTGAAGCGATTCAATACTACCTATTAAAAGCATCTGTTGGTCTAGCAAAA
GAGCAAGGTGCATGTCCTGCTTTCAATGAAACAACCTATGCTCAAGGTATTCTACCTATCGATTCTTACAAGAAAGATAT
CGATGCTATCTGTAATGAAGAATTGCATTTAGATTGGGAAACATTGCGTGAAGAGATCAAGACACACGGTCTACGTAACT
CTACACTATCTGCACTGATGCCATCTGAGACGTCTTCTCAAATCTCAAATGCAACTAACGGTATTGAACCACCACGTGGT
TTTGTATCAGTTAAAGCATCAAAAGATGGTATTTTGAAACAAGTTGTTCCTGATTTTGCGAATCTGAAAAACAACTATGA
ACTACTTTGGAACATTGGTTCAAACGATGGCTATCTACAGCTTGTAGGTATCATGCAAAAATTCGTTGACCAAGCAATTT
CAGCGAATACTAATTACGATCCAAACATTCAACCAAATGGCAAAGTGCCAATGAAGCTGTTATTAAAAGACCTTCTAACG
GCTTATAAGCTTGGTCTTAAAACACTTTACTACCATAACACTCGTGATGGTGCTAGCGACTCACAAGACGACCTAGGACA
AGATGACGATTGTGCAGGCGGTGGTTGTAAGATCTAA

Upstream 100 bases:

>100_bases
TGTTTATTTCAAAGCGCACCACTATGAGCTTGATCACAAATTAACAACGAAAATTACAAAAATTACATCTTCGTTCTTTA
TTGAAAGGAAAAGTAGACAA

Downstream 100 bases:

>100_bases
GTTTTCACATTAATATTTAGCCCGCTTTCATTAGCGGGCTTATCATCTTTAGTTTCAACATCTTTAATGGTTAATCCAAT
ATGTACCTAAGTTCGTATAG

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Protein B1; Ribonucleoside-diphosphate reductase 1 R1 subunit; Ribonucleotide reductase 1 [H]

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MTQQLTVTKRNGSTEKINLDKIHKVITWAAEGLDNVSVSQVEMKSHIQFYDGMKTSDIHETIIKAAADLISEDTPDYQYL
AARLSVFHLRKKAYGQFEPPTLFNHVLNLIELGKYDKHIVEDYTQEEFALMDQMIDHNRDMDFSYAAVKQLEGKYFVQNR
VTGAIYESAQFLYILVAACLFAKYPKDTRLDYIKRFYDATSQFKISLPTPIMSGVRTPTRQFSSCVLIECDDSLDSINAT
AGSIVRYVSQRAGIGINAGRIRALGSEIRDGEAFHTGCIPFYKYFQTAVKCCSQGGVRGGAATLFYPIWHREVESLLVLK
NNRGVEENRVRHMDYGVQINKLMYTRLIKGGNISLFSPSDVPGLYDAFFADQAEFERLYEKYEQDTSIKRETVKAIELFS
LLMQERASTGRIYIQNVDHCNTHSPFDPSVAPVRQSNLCLEIALPTKPLTHVNDEEGEIALCTLSAFNLGAIEKLEELEE
LAELAVRALDALLDYQDYPLPAAYKSTMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLTHKTFEAIQYYLLKASVGLAK
EQGACPAFNETTYAQGILPIDSYKKDIDAICNEELHLDWETLREEIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRG
FVSVKASKDGILKQVVPDFANLKNNYELLWNIGSNDGYLQLVGIMQKFVDQAISANTNYDPNIQPNGKVPMKLLLKDLLT
AYKLGLKTLYYHNTRDGASDSQDDLGQDDDCAGGGCKI

Sequences:

>Translated_758_residues
MTQQLTVTKRNGSTEKINLDKIHKVITWAAEGLDNVSVSQVEMKSHIQFYDGMKTSDIHETIIKAAADLISEDTPDYQYL
AARLSVFHLRKKAYGQFEPPTLFNHVLNLIELGKYDKHIVEDYTQEEFALMDQMIDHNRDMDFSYAAVKQLEGKYFVQNR
VTGAIYESAQFLYILVAACLFAKYPKDTRLDYIKRFYDATSQFKISLPTPIMSGVRTPTRQFSSCVLIECDDSLDSINAT
AGSIVRYVSQRAGIGINAGRIRALGSEIRDGEAFHTGCIPFYKYFQTAVKCCSQGGVRGGAATLFYPIWHREVESLLVLK
NNRGVEENRVRHMDYGVQINKLMYTRLIKGGNISLFSPSDVPGLYDAFFADQAEFERLYEKYEQDTSIKRETVKAIELFS
LLMQERASTGRIYIQNVDHCNTHSPFDPSVAPVRQSNLCLEIALPTKPLTHVNDEEGEIALCTLSAFNLGAIEKLEELEE
LAELAVRALDALLDYQDYPLPAAYKSTMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLTHKTFEAIQYYLLKASVGLAK
EQGACPAFNETTYAQGILPIDSYKKDIDAICNEELHLDWETLREEIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRG
FVSVKASKDGILKQVVPDFANLKNNYELLWNIGSNDGYLQLVGIMQKFVDQAISANTNYDPNIQPNGKVPMKLLLKDLLT
AYKLGLKTLYYHNTRDGASDSQDDLGQDDDCAGGGCKI
>Mature_757_residues
TQQLTVTKRNGSTEKINLDKIHKVITWAAEGLDNVSVSQVEMKSHIQFYDGMKTSDIHETIIKAAADLISEDTPDYQYLA
ARLSVFHLRKKAYGQFEPPTLFNHVLNLIELGKYDKHIVEDYTQEEFALMDQMIDHNRDMDFSYAAVKQLEGKYFVQNRV
TGAIYESAQFLYILVAACLFAKYPKDTRLDYIKRFYDATSQFKISLPTPIMSGVRTPTRQFSSCVLIECDDSLDSINATA
GSIVRYVSQRAGIGINAGRIRALGSEIRDGEAFHTGCIPFYKYFQTAVKCCSQGGVRGGAATLFYPIWHREVESLLVLKN
NRGVEENRVRHMDYGVQINKLMYTRLIKGGNISLFSPSDVPGLYDAFFADQAEFERLYEKYEQDTSIKRETVKAIELFSL
LMQERASTGRIYIQNVDHCNTHSPFDPSVAPVRQSNLCLEIALPTKPLTHVNDEEGEIALCTLSAFNLGAIEKLEELEEL
AELAVRALDALLDYQDYPLPAAYKSTMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLTHKTFEAIQYYLLKASVGLAKE
QGACPAFNETTYAQGILPIDSYKKDIDAICNEELHLDWETLREEIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRGF
VSVKASKDGILKQVVPDFANLKNNYELLWNIGSNDGYLQLVGIMQKFVDQAISANTNYDPNIQPNGKVPMKLLLKDLLTA
YKLGLKTLYYHNTRDGASDSQDDLGQDDDCAGGGCKI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1 contains the binding sites for both substrates and allosteric effectors and carries out the actual reduction

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-cone domain [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=793, Percent_Identity=28.1210592686003, Blast_Score=243, Evalue=7e-64,
Organism=Escherichia coli, GI2367133, Length=761, Percent_Identity=81.8659658344284, Blast_Score=1323, Evalue=0.0,
Organism=Escherichia coli, GI1789030, Length=567, Percent_Identity=24.6913580246914, Blast_Score=149, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17555242, Length=648, Percent_Identity=29.1666666666667, Blast_Score=227, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6322123, Length=651, Percent_Identity=29.0322580645161, Blast_Score=244, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6320914, Length=653, Percent_Identity=28.9433384379786, Blast_Score=235, Evalue=2e-62,
Organism=Drosophila melanogaster, GI17352481, Length=792, Percent_Identity=28.4090909090909, Blast_Score=261, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005144
- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926 [H]

Pfam domain/function: PF03477 ATP-cone; PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 84946; Mature: 84815

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS51161 ATP_CONE ; PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQLTVTKRNGSTEKINLDKIHKVITWAAEGLDNVSVSQVEMKSHIQFYDGMKTSDIHE
CCCCEEEEECCCCCCEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
TIIKAAADLISEDTPDYQYLAARLSVFHLRKKAYGQFEPPTLFNHVLNLIELGKYDKHIV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHH
EDYTQEEFALMDQMIDHNRDMDFSYAAVKQLEGKYFVQNRVTGAIYESAQFLYILVAACL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEHHCCHHHHHHHHHHHHHHHHHHH
FAKYPKDTRLDYIKRFYDATSQFKISLPTPIMSGVRTPTRQFSSCVLIECDDSLDSINAT
HHCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHCCCCCHHHHCCEEEEEECCCCHHHHHH
AGSIVRYVSQRAGIGINAGRIRALGSEIRDGEAFHTGCIPFYKYFQTAVKCCSQGGVRGG
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
AATLFYPIWHREVESLLVLKNNRGVEENRVRHMDYGVQINKLMYTRLIKGGNISLFSPSD
CHHEEHHHHHHHHHHEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCC
VPGLYDAFFADQAEFERLYEKYEQDTSIKRETVKAIELFSLLMQERASTGRIYIQNVDHC
CCHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NTHSPFDPSVAPVRQSNLCLEIALPTKPLTHVNDEEGEIALCTLSAFNLGAIEKLEELEE
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
LAELAVRALDALLDYQDYPLPAAYKSTMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLT
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCC
HKTFEAIQYYLLKASVGLAKEQGACPAFNETTYAQGILPIDSYKKDIDAICNEELHLDWE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHCCCCCCCCHH
TLREEIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRGFVSVKASKDGILKQVVPDFA
HHHHHHHHCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHH
NLKNNYELLWNIGSNDGYLQLVGIMQKFVDQAISANTNYDPNIQPNGKVPMKLLLKDLLT
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
AYKLGLKTLYYHNTRDGASDSQDDLGQDDDCAGGGCKI
HHHHHHHHEEEECCCCCCCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQQLTVTKRNGSTEKINLDKIHKVITWAAEGLDNVSVSQVEMKSHIQFYDGMKTSDIHE
CCCEEEEECCCCCCEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
TIIKAAADLISEDTPDYQYLAARLSVFHLRKKAYGQFEPPTLFNHVLNLIELGKYDKHIV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHH
EDYTQEEFALMDQMIDHNRDMDFSYAAVKQLEGKYFVQNRVTGAIYESAQFLYILVAACL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEHHCCHHHHHHHHHHHHHHHHHHH
FAKYPKDTRLDYIKRFYDATSQFKISLPTPIMSGVRTPTRQFSSCVLIECDDSLDSINAT
HHCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHCCCCCHHHHCCEEEEEECCCCHHHHHH
AGSIVRYVSQRAGIGINAGRIRALGSEIRDGEAFHTGCIPFYKYFQTAVKCCSQGGVRGG
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
AATLFYPIWHREVESLLVLKNNRGVEENRVRHMDYGVQINKLMYTRLIKGGNISLFSPSD
CHHEEHHHHHHHHHHEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCC
VPGLYDAFFADQAEFERLYEKYEQDTSIKRETVKAIELFSLLMQERASTGRIYIQNVDHC
CCHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NTHSPFDPSVAPVRQSNLCLEIALPTKPLTHVNDEEGEIALCTLSAFNLGAIEKLEELEE
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
LAELAVRALDALLDYQDYPLPAAYKSTMNRRTLGVGVINYAYYLAKNGVKYSDGSANGLT
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCC
HKTFEAIQYYLLKASVGLAKEQGACPAFNETTYAQGILPIDSYKKDIDAICNEELHLDWE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHCCCCCCCCHH
TLREEIKTHGLRNSTLSALMPSETSSQISNATNGIEPPRGFVSVKASKDGILKQVVPDFA
HHHHHHHHCCCCCCHHHHHCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHH
NLKNNYELLWNIGSNDGYLQLVGIMQKFVDQAISANTNYDPNIQPNGKVPMKLLLKDLLT
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
AYKLGLKTLYYHNTRDGASDSQDDLGQDDDCAGGGCKI
HHHHHHHHEEEECCCCCCCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6087316; 3287341; 9205837; 9278503; 3894026; 2663852; 8702814; 8052308; 9309223; 10493864 [H]