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The map label for this gene is ptrB [C]

Identifier: 59711729

GI number: 59711729

Start: 1237914

End: 1239950

Strand: Direct

Name: ptrB [C]

Synonym: VF_1122

Alternate gene names: 59711729

Gene position: 1237914-1239950 (Clockwise)

Preceding gene: 59711727

Following gene: 59711730

Centisome position: 42.72

GC content: 40.8

Gene sequence:

>2037_bases
ATGATTTATCCTTTCACAAAACAAGACTCAACTGTTGACCACTATTTTTCACATGCTATTGCTGATCCATACCGTTGGCT
TGAAGATGACAGAAGTGAAGAAACAGAAGCGTGGGTGAATAAACAAAATGACGTCACTTTCGATTACCTTTCTCACATTG
ATTTTCGTGACGATATCCGAGATTTAATCGCAAAAGGTCAGGATTACCAAAAAACGTCACAACCATTTAAGCGTGGTGAA
TACACCTATTTTTATCAAAATGATGGCTTACAAAATCAGAGTGTTCTGTATCGCTCAAAAGAAGGGAAGGACGTTGAGGT
TTTTCTTGATCCAAATACGTTCAGCGAAGAGGGGACGACTTCTTTAAGTGTCGTTAGTTTTTCAAAAGATGCTTCATTAG
TCGCGTATTCGATTTCAGAAGCGGGCAGTGATTGGCGCAAAATCATCGTATTAGATGCTGAAACCAAAGAGCAAATTGAA
GCGCCAATTGTCGATGCTAAATTTACTGGTATTTCATGGTTAGGTTCAAAAGGCTTTTACTACTCAAGCTATGATAAGCC
AGAAGGCAGTGAATTATCAGCAAGAACAGAGCAACATAAACTCTATTTTCATGAGATAGGCAAACCACAAGCCGACGATG
TTTTGGTGTTTGGTGGAAGTGAAGAAGAAAAACATCGGTATGTCAGTGGCTATACAACGGAAGATGATGCGTATTTAGTG
ATCTCAGCATCTACATCAACATCGGGTAATAAACTGTTTATCGAACCATTAAACAGAGACAGTTCATATCGATTAACGGT
GCTTGATCATACGGATTCAGATACCTATGTGATTGAGAATGAAGACACACACCTGCTGATGTTTACGAACCTAAATGCAC
CTAATGGCAAAGTGGTATCTTATGATGTTGTTACCGGTGAGTGGACTGACATTATCCCTGAACAACCACAGCCATTAGAT
ATAAGCGTTGGTGGCGGTACCTTGTTTGCGACTTATATGGTTGATGTACTGAGCCAAGTGAAGCAATACGATTTTTCAGG
CAATCTATTACGCGAAATTGAACTACCGGGTGAAGGCCAAGCAACGGGGTTCTCAGGTAAAAGAGAACAAACCGATCTAT
TCTTTACCTTTACTAACTACGTTACACCACCAACGATTTATCAATTTGATGTAAAAACCGGCGAGACAGAACTGTATTTA
GCGTCTGCTTCCCCTTTTGACTCGGAGAAGTTTGAATCGAAACAAGTGTTTTATACATCAAAAGACGGTACGCAAATTCC
AATGCTTATCTCTTATCGTAAAGGCATTGAGCTTGATGGCAGTAACCCAACGATTCTATATGGATATGGTGGTTTTAATG
TCAGCTTAACACCAAACTTTAGTGGCGTAATGGCCAGTTGGTTAGAGCTTGGCGGTGTGTATGCAATTCCCAATATTCGC
GGTGGTGGTGAATATGGAAAAGAGTGGCATAAGGCAGGAACACAGCAACAAAAACAAAATGTATTTGATGACTTTATTGC
TGCTGCCGAATTTTTAATCGCAGAAAAATATACCGACTCATCACGTTTGGCAATTCGTGGTGGTTCAAACGGTGGATTAC
TGGTTGGTGCGTGCATGACTCAGCGACCAGAACTATTTAAAGTTGCTCTGCCAGCGGTTGGTGTTCTGGATATGCTTCGT
TATCACACCTTTACTTCTGGTGAAGGTTGGGCGTATGACTACGGTACTTCAGAGCAAAGTGAAGAGATGTTTAACTATCT
ATTAAACTATTCACCAGTGCATAACGTGAAAGAGGGCGTTGAATACCCTGCAACATTGGTAACAACCGCTGATCATGATG
ATCGTGTTGTTCCTGCTCACTCTTATAAGTTTATTTCTGAGCTGCAAGCCAAGCAATCAGGCGATAACCCTGTGTTAATC
CGCATCGATGTTAATGCCGGTCATGGTGCAGGTATGCCAATATCGAAATCAATGGATTTAATGGCGGATGTTTACGCATT
TACCTTAAGTAATATGAAGGTGAATCCGTTTGAGTAA

Upstream 100 bases:

>100_bases
TCAATAACTAGTCTTAAACCACCAAACAGTTGCATTCCTATTTTTAATGGGCGTATTCTACAAACAGATAACACAAACTA
ATTATAAATAGGGATGCATT

Downstream 100 bases:

>100_bases
ATGAGTCAATTTGGTTTTGTTTATCTTTCAATAAATTAACAATATAGCGTTTATCAATAATGTAGAGCCACATGCAGAAT
TGTGTGTGGCTTTTATGTGT

Product: prolyl endopeptidase

Products: NA

Alternate protein names: PE; Post-proline cleaving enzyme [H]

Number of amino acids: Translated: 678; Mature: 678

Protein sequence:

>678_residues
MIYPFTKQDSTVDHYFSHAIADPYRWLEDDRSEETEAWVNKQNDVTFDYLSHIDFRDDIRDLIAKGQDYQKTSQPFKRGE
YTYFYQNDGLQNQSVLYRSKEGKDVEVFLDPNTFSEEGTTSLSVVSFSKDASLVAYSISEAGSDWRKIIVLDAETKEQIE
APIVDAKFTGISWLGSKGFYYSSYDKPEGSELSARTEQHKLYFHEIGKPQADDVLVFGGSEEEKHRYVSGYTTEDDAYLV
ISASTSTSGNKLFIEPLNRDSSYRLTVLDHTDSDTYVIENEDTHLLMFTNLNAPNGKVVSYDVVTGEWTDIIPEQPQPLD
ISVGGGTLFATYMVDVLSQVKQYDFSGNLLREIELPGEGQATGFSGKREQTDLFFTFTNYVTPPTIYQFDVKTGETELYL
ASASPFDSEKFESKQVFYTSKDGTQIPMLISYRKGIELDGSNPTILYGYGGFNVSLTPNFSGVMASWLELGGVYAIPNIR
GGGEYGKEWHKAGTQQQKQNVFDDFIAAAEFLIAEKYTDSSRLAIRGGSNGGLLVGACMTQRPELFKVALPAVGVLDMLR
YHTFTSGEGWAYDYGTSEQSEEMFNYLLNYSPVHNVKEGVEYPATLVTTADHDDRVVPAHSYKFISELQAKQSGDNPVLI
RIDVNAGHGAGMPISKSMDLMADVYAFTLSNMKVNPFE

Sequences:

>Translated_678_residues
MIYPFTKQDSTVDHYFSHAIADPYRWLEDDRSEETEAWVNKQNDVTFDYLSHIDFRDDIRDLIAKGQDYQKTSQPFKRGE
YTYFYQNDGLQNQSVLYRSKEGKDVEVFLDPNTFSEEGTTSLSVVSFSKDASLVAYSISEAGSDWRKIIVLDAETKEQIE
APIVDAKFTGISWLGSKGFYYSSYDKPEGSELSARTEQHKLYFHEIGKPQADDVLVFGGSEEEKHRYVSGYTTEDDAYLV
ISASTSTSGNKLFIEPLNRDSSYRLTVLDHTDSDTYVIENEDTHLLMFTNLNAPNGKVVSYDVVTGEWTDIIPEQPQPLD
ISVGGGTLFATYMVDVLSQVKQYDFSGNLLREIELPGEGQATGFSGKREQTDLFFTFTNYVTPPTIYQFDVKTGETELYL
ASASPFDSEKFESKQVFYTSKDGTQIPMLISYRKGIELDGSNPTILYGYGGFNVSLTPNFSGVMASWLELGGVYAIPNIR
GGGEYGKEWHKAGTQQQKQNVFDDFIAAAEFLIAEKYTDSSRLAIRGGSNGGLLVGACMTQRPELFKVALPAVGVLDMLR
YHTFTSGEGWAYDYGTSEQSEEMFNYLLNYSPVHNVKEGVEYPATLVTTADHDDRVVPAHSYKFISELQAKQSGDNPVLI
RIDVNAGHGAGMPISKSMDLMADVYAFTLSNMKVNPFE
>Mature_678_residues
MIYPFTKQDSTVDHYFSHAIADPYRWLEDDRSEETEAWVNKQNDVTFDYLSHIDFRDDIRDLIAKGQDYQKTSQPFKRGE
YTYFYQNDGLQNQSVLYRSKEGKDVEVFLDPNTFSEEGTTSLSVVSFSKDASLVAYSISEAGSDWRKIIVLDAETKEQIE
APIVDAKFTGISWLGSKGFYYSSYDKPEGSELSARTEQHKLYFHEIGKPQADDVLVFGGSEEEKHRYVSGYTTEDDAYLV
ISASTSTSGNKLFIEPLNRDSSYRLTVLDHTDSDTYVIENEDTHLLMFTNLNAPNGKVVSYDVVTGEWTDIIPEQPQPLD
ISVGGGTLFATYMVDVLSQVKQYDFSGNLLREIELPGEGQATGFSGKREQTDLFFTFTNYVTPPTIYQFDVKTGETELYL
ASASPFDSEKFESKQVFYTSKDGTQIPMLISYRKGIELDGSNPTILYGYGGFNVSLTPNFSGVMASWLELGGVYAIPNIR
GGGEYGKEWHKAGTQQQKQNVFDDFIAAAEFLIAEKYTDSSRLAIRGGSNGGLLVGACMTQRPELFKVALPAVGVLDMLR
YHTFTSGEGWAYDYGTSEQSEEMFNYLLNYSPVHNVKEGVEYPATLVTTADHDDRVVPAHSYKFISELQAKQSGDNPVLI
RIDVNAGHGAGMPISKSMDLMADVYAFTLSNMKVNPFE

Specific function: Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Has an absolute requirement for an X-Pro bond in the trans configuration immediately preceding the Pro-Y scissible bond [H]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9A family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=710, Percent_Identity=40, Blast_Score=494, Evalue=1e-139,
Organism=Homo sapiens, GI284172420, Length=592, Percent_Identity=21.4527027027027, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI284172413, Length=592, Percent_Identity=21.4527027027027, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI70778815, Length=592, Percent_Identity=21.4527027027027, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI284172438, Length=592, Percent_Identity=21.4527027027027, Blast_Score=103, Evalue=7e-22,
Organism=Homo sapiens, GI284172431, Length=592, Percent_Identity=21.4527027027027, Blast_Score=103, Evalue=7e-22,
Organism=Homo sapiens, GI108860686, Length=249, Percent_Identity=26.1044176706827, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI108860692, Length=199, Percent_Identity=28.1407035175879, Blast_Score=92, Evalue=2e-18,
Organism=Escherichia coli, GI1788150, Length=673, Percent_Identity=24.665676077266, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24583414, Length=715, Percent_Identity=40.4195804195804, Blast_Score=482, Evalue=1e-136,
Organism=Drosophila melanogaster, GI221510989, Length=711, Percent_Identity=38.8185654008439, Blast_Score=466, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002471
- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: =3.4.21.26 [H]

Molecular weight: Translated: 75975; Mature: 75975

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00708 PRO_ENDOPEP_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYPFTKQDSTVDHYFSHAIADPYRWLEDDRSEETEAWVNKQNDVTFDYLSHIDFRDDIR
CCCCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCHHHHHHCCCCCEEHHHHHCCCCHHHHH
DLIAKGQDYQKTSQPFKRGEYTYFYQNDGLQNQSVLYRSKEGKDVEVFLDPNTFSEEGTT
HHHHCCCCCHHHCCHHHCCCEEEEEECCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCC
SLSVVSFSKDASLVAYSISEAGSDWRKIIVLDAETKEQIEAPIVDAKFTGISWLGSKGFY
EEEEEEECCCCEEEEEEHHHCCCCEEEEEEEECCCHHHHCCCEEEEEECCEEEECCCCCE
YSSYDKPEGSELSARTEQHKLYFHEIGKPQADDVLVFGGSEEEKHRYVSGYTTEDDAYLV
ECCCCCCCCCCCCCCHHHHEEEEECCCCCCCCCEEEECCCCHHCCEEECCEECCCCCEEE
ISASTSTSGNKLFIEPLNRDSSYRLTVLDHTDSDTYVIENEDTHLLMFTNLNAPNGKVVS
EEECCCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCEEE
YDVVTGEWTDIIPEQPQPLDISVGGGTLFATYMVDVLSQVKQYDFSGNLLREIELPGEGQ
EEEEECCHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
ATGFSGKREQTDLFFTFTNYVTPPTIYQFDVKTGETELYLASASPFDSEKFESKQVFYTS
CCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCEEEEEC
KDGTQIPMLISYRKGIELDGSNPTILYGYGGFNVSLTPNFSGVMASWLELGGVYAIPNIR
CCCCCCCEEEEECCCEEECCCCCEEEEEECCEEEEECCCCHHHHHHHHHHCCEEEECCCC
GGGEYGKEWHKAGTQQQKQNVFDDFIAAAEFLIAEKYTDSSRLAIRGGSNGGLLVGACMT
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECC
QRPELFKVALPAVGVLDMLRYHTFTSGEGWAYDYGTSEQSEEMFNYLLNYSPVHNVKEGV
CCCCCEEEHHHHHHHHHHHHHHEECCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHCC
EYPATLVTTADHDDRVVPAHSYKFISELQAKQSGDNPVLIRIDVNAGHGAGMPISKSMDL
CCCEEEEEECCCCCCEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHH
MADVYAFTLSNMKVNPFE
HHHHHHHHCCCEEECCCC
>Mature Secondary Structure
MIYPFTKQDSTVDHYFSHAIADPYRWLEDDRSEETEAWVNKQNDVTFDYLSHIDFRDDIR
CCCCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCHHHHHHCCCCCEEHHHHHCCCCHHHHH
DLIAKGQDYQKTSQPFKRGEYTYFYQNDGLQNQSVLYRSKEGKDVEVFLDPNTFSEEGTT
HHHHCCCCCHHHCCHHHCCCEEEEEECCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCC
SLSVVSFSKDASLVAYSISEAGSDWRKIIVLDAETKEQIEAPIVDAKFTGISWLGSKGFY
EEEEEEECCCCEEEEEEHHHCCCCEEEEEEEECCCHHHHCCCEEEEEECCEEEECCCCCE
YSSYDKPEGSELSARTEQHKLYFHEIGKPQADDVLVFGGSEEEKHRYVSGYTTEDDAYLV
ECCCCCCCCCCCCCCHHHHEEEEECCCCCCCCCEEEECCCCHHCCEEECCEECCCCCEEE
ISASTSTSGNKLFIEPLNRDSSYRLTVLDHTDSDTYVIENEDTHLLMFTNLNAPNGKVVS
EEECCCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCEEE
YDVVTGEWTDIIPEQPQPLDISVGGGTLFATYMVDVLSQVKQYDFSGNLLREIELPGEGQ
EEEEECCHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
ATGFSGKREQTDLFFTFTNYVTPPTIYQFDVKTGETELYLASASPFDSEKFESKQVFYTS
CCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCEEEEEC
KDGTQIPMLISYRKGIELDGSNPTILYGYGGFNVSLTPNFSGVMASWLELGGVYAIPNIR
CCCCCCCEEEEECCCEEECCCCCEEEEEECCEEEEECCCCHHHHHHHHHHCCEEEECCCC
GGGEYGKEWHKAGTQQQKQNVFDDFIAAAEFLIAEKYTDSSRLAIRGGSNGGLLVGACMT
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECC
QRPELFKVALPAVGVLDMLRYHTFTSGEGWAYDYGTSEQSEEMFNYLLNYSPVHNVKEGV
CCCCCEEEHHHHHHHHHHHHHHEECCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHCC
EYPATLVTTADHDDRVVPAHSYKFISELQAKQSGDNPVLIRIDVNAGHGAGMPISKSMDL
CCCEEEEEECCCCCCEECCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHH
MADVYAFTLSNMKVNPFE
HHHHHHHHCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370677 [H]