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The map label for this gene is gadA [H]

Identifier: 59711671

GI number: 59711671

Start: 1176030

End: 1177424

Strand: Direct

Name: gadA [H]

Synonym: VF_1064

Alternate gene names: 59711671

Gene position: 1176030-1177424 (Clockwise)

Preceding gene: 59711670

Following gene: 59711672

Centisome position: 40.59

GC content: 41.0

Gene sequence:

>1395_bases
ATGCCTCTTCATTCCAAAAACGCAGTCAGAGATGATCTTCTTGATGATATTTATTCATCAGCTGACCTTTCGTTATCCAT
GCCAAAATATAAAATGCCTGAACAAGAACATGACCCACGTCATGCCTACCAAGTCATTCATGATGAGCTCATGATGGATG
GTAATTCTCGACAGAACTTAGCAACGTTTTGCCAAACTTGGGTTGAAGATGAAGTCCATAAATTAATGGATGAATGTATC
GATAAAAATATGATCGATAAAGATGAATACCCTCAAACTGCTGAATTAGAATCTCGCTGTGTGCATATGCTAGCGGATTT
ATGGAACTCTCCTGACGCAGAAAACACACTAGGTTGTTCCACTACAGGTTCAAGTGAAGCCGCTATGCTAGGTGGTATGG
CATTAAAATGGGCTTGGCGAGAAAAAATGAAAAAACTTGGCAAACCAACAGATAAGCCAAATATGATCTGTGGTCCCGTT
CAAGTATGTTGGCATAAATTTGCACGTTATTGGGATATTGAATTACGTGAGATCCCAATGGAAGGCGATCGCTTGATCAT
GACGCCTGAAGAAGTGATTAAACGTTGTGATGAAAATACCATTGGTGTTGTTCCAACACTGGGGGTGACTTTTACTTGTC
AATATGAGCCTGTGAAAGCGGTACATGAAGCGCTAGATAAGTTACAAGAAGAGACGGGTTTAGATATTCCAATGCACATT
GATGCGGCGAGTGGTGGTTTCTTAGCTCCGTTCTGTGATCCTGATTTAGAGTGGGACTTCCGTCTTCCTCGAGTTAAATC
GATTAATGCATCAGGTCATAAATTTGGCCTAAGCCCACTAGGTGTGGGTTGGGTAATTTGGCGTGACGCATCAGCCTTGC
ATGAAGATTTGATTTTTAATGTGAACTATTTAGGTGGCAATATGCCGACCTTTGCATTGAACTTCTCTCGCCCAGGTGGC
CAAATTGTAGCGCAATACTACAATTTCCTACGTTTAGGAAAAGAAGGGTATCGTAAGATCCACCAAGCGTGTTATGACAC
TGCTGTTTATTTATCATCTGAAATTGAAAAACTGGGTATGTTTGAAATTATCTATGATGGTAAAGGTGGTATTCCAGCAA
TGAGTTGGTCATTGAAGGAAGGAGTTGATCCTGGTTTTAATTTGTTTGACCTATCGGATCGAATTCGTTCTCGTGGTTGG
CAAATTGCGGCTTATGCAATGCCACCAAAACGTGAAGATTTAGTCATCATGCGTATTTTAGTACGTCATGGATTCAGTCG
AGATCAAGCGGATTTATTGGTTGCGGACTTAAAACATTGCGTTGAGTTTTTTGCTAAGCATCCTATTTCCCATGGTAGTG
ATGAGTTGGAATCATCAGGATTTAACCACGGCTAA

Upstream 100 bases:

>100_bases
AATTGACAGTATTGTAAATTGCTCATTAATTAAGGCCGAATTTAGTTAATTCGACAGTTTGTATTCTTATTGTATGTTCA
ATTCAATGGAGCTAAAACGT

Downstream 100 bases:

>100_bases
TCCCTTTCGTCGTGATATAGACATTTTGCTTTTTAGATCCTCTATTGTTCTGTAGAGGATCTCTTCTTCCTAAAAATATA
GGAAATAATTATGAATATCG

Product: glutamate decarboxylase A, PLP-dependent

Products: NA

Alternate protein names: GAD-alpha [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MPLHSKNAVRDDLLDDIYSSADLSLSMPKYKMPEQEHDPRHAYQVIHDELMMDGNSRQNLATFCQTWVEDEVHKLMDECI
DKNMIDKDEYPQTAELESRCVHMLADLWNSPDAENTLGCSTTGSSEAAMLGGMALKWAWREKMKKLGKPTDKPNMICGPV
QVCWHKFARYWDIELREIPMEGDRLIMTPEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHEALDKLQEETGLDIPMHI
DAASGGFLAPFCDPDLEWDFRLPRVKSINASGHKFGLSPLGVGWVIWRDASALHEDLIFNVNYLGGNMPTFALNFSRPGG
QIVAQYYNFLRLGKEGYRKIHQACYDTAVYLSSEIEKLGMFEIIYDGKGGIPAMSWSLKEGVDPGFNLFDLSDRIRSRGW
QIAAYAMPPKREDLVIMRILVRHGFSRDQADLLVADLKHCVEFFAKHPISHGSDELESSGFNHG

Sequences:

>Translated_464_residues
MPLHSKNAVRDDLLDDIYSSADLSLSMPKYKMPEQEHDPRHAYQVIHDELMMDGNSRQNLATFCQTWVEDEVHKLMDECI
DKNMIDKDEYPQTAELESRCVHMLADLWNSPDAENTLGCSTTGSSEAAMLGGMALKWAWREKMKKLGKPTDKPNMICGPV
QVCWHKFARYWDIELREIPMEGDRLIMTPEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHEALDKLQEETGLDIPMHI
DAASGGFLAPFCDPDLEWDFRLPRVKSINASGHKFGLSPLGVGWVIWRDASALHEDLIFNVNYLGGNMPTFALNFSRPGG
QIVAQYYNFLRLGKEGYRKIHQACYDTAVYLSSEIEKLGMFEIIYDGKGGIPAMSWSLKEGVDPGFNLFDLSDRIRSRGW
QIAAYAMPPKREDLVIMRILVRHGFSRDQADLLVADLKHCVEFFAKHPISHGSDELESSGFNHG
>Mature_463_residues
PLHSKNAVRDDLLDDIYSSADLSLSMPKYKMPEQEHDPRHAYQVIHDELMMDGNSRQNLATFCQTWVEDEVHKLMDECID
KNMIDKDEYPQTAELESRCVHMLADLWNSPDAENTLGCSTTGSSEAAMLGGMALKWAWREKMKKLGKPTDKPNMICGPVQ
VCWHKFARYWDIELREIPMEGDRLIMTPEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHEALDKLQEETGLDIPMHID
AASGGFLAPFCDPDLEWDFRLPRVKSINASGHKFGLSPLGVGWVIWRDASALHEDLIFNVNYLGGNMPTFALNFSRPGGQ
IVAQYYNFLRLGKEGYRKIHQACYDTAVYLSSEIEKLGMFEIIYDGKGGIPAMSWSLKEGVDPGFNLFDLSDRIRSRGWQ
IAAYAMPPKREDLVIMRILVRHGFSRDQADLLVADLKHCVEFFAKHPISHGSDELESSGFNHG

Specific function: Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit th

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=379, Percent_Identity=26.3852242744063, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1787769, Length=459, Percent_Identity=57.7342047930283, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI1789934, Length=458, Percent_Identity=57.8602620087336, Blast_Score=566, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI17557272, Length=376, Percent_Identity=25.7978723404255, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17543922, Length=341, Percent_Identity=24.633431085044, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25148342, Length=321, Percent_Identity=22.4299065420561, Blast_Score=85, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6323905, Length=457, Percent_Identity=40.0437636761488, Blast_Score=313, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6320500, Length=339, Percent_Identity=25.3687315634218, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21355963, Length=373, Percent_Identity=26.0053619302949, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24654344, Length=373, Percent_Identity=26.0053619302949, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR021115 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 52583; Mature: 52451

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLHSKNAVRDDLLDDIYSSADLSLSMPKYKMPEQEHDPRHAYQVIHDELMMDGNSRQNL
CCCCCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
ATFCQTWVEDEVHKLMDECIDKNMIDKDEYPQTAELESRCVHMLADLWNSPDAENTLGCS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TTGSSEAAMLGGMALKWAWREKMKKLGKPTDKPNMICGPVQVCWHKFARYWDIELREIPM
CCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHCCCEEEECCC
EGDRLIMTPEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHEALDKLQEETGLDIPMHI
CCCEEEECHHHHHHHCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEE
DAASGGFLAPFCDPDLEWDFRLPRVKSINASGHKFGLSPLGVGWVIWRDASALHEDLIFN
ECCCCCEECCCCCCCCCCCCCCCCEEECCCCCCEECCCCCCCCEEEECCHHHHHHHHEEE
VNYLGGNMPTFALNFSRPGGQIVAQYYNFLRLGKEGYRKIHQACYDTAVYLSSEIEKLGM
EEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCC
FEIIYDGKGGIPAMSWSLKEGVDPGFNLFDLSDRIRSRGWQIAAYAMPPKREDLVIMRIL
EEEEECCCCCCCCCCCCHHHCCCCCCCEECHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
VRHGFSRDQADLLVADLKHCVEFFAKHPISHGSDELESSGFNHG
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
PLHSKNAVRDDLLDDIYSSADLSLSMPKYKMPEQEHDPRHAYQVIHDELMMDGNSRQNL
CCCCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
ATFCQTWVEDEVHKLMDECIDKNMIDKDEYPQTAELESRCVHMLADLWNSPDAENTLGCS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
TTGSSEAAMLGGMALKWAWREKMKKLGKPTDKPNMICGPVQVCWHKFARYWDIELREIPM
CCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHCCCEEEECCC
EGDRLIMTPEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHEALDKLQEETGLDIPMHI
CCCEEEECHHHHHHHCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEE
DAASGGFLAPFCDPDLEWDFRLPRVKSINASGHKFGLSPLGVGWVIWRDASALHEDLIFN
ECCCCCEECCCCCCCCCCCCCCCCEEECCCCCCEECCCCCCCCEEEECCHHHHHHHHEEE
VNYLGGNMPTFALNFSRPGGQIVAQYYNFLRLGKEGYRKIHQACYDTAVYLSSEIEKLGM
EEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCC
FEIIYDGKGGIPAMSWSLKEGVDPGFNLFDLSDRIRSRGWQIAAYAMPPKREDLVIMRIL
EEEEECCCCCCCCCCCCHHHCCCCCCCEECHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
VRHGFSRDQADLLVADLKHCVEFFAKHPISHGSDELESSGFNHG
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]