Click here to switch to the map view.

The map label for this gene is hisF [H]

Identifier: 59711625

GI number: 59711625

Start: 1124892

End: 1125665

Strand: Direct

Name: hisF [H]

Synonym: VF_1018

Alternate gene names: 59711625

Gene position: 1124892-1125665 (Clockwise)

Preceding gene: 59711624

Following gene: 59711626

Centisome position: 38.82

GC content: 41.86

Gene sequence:

>774_bases
ATGTTGGCAAAGCGAATAATTCCGTGCCTTGATGTAAAAGATGGTCAAGTAGTAAAAGGCGTTCAGTTTCGTAACCATGA
AATCATCGGTGATATTGTCCCCCTAGCACAGCGTTATGCGGAAGAGGGTGCAGATGAGCTCGTCTTCTACGATATTACCG
CATCAAGTGATGGTCGTGTGGTAGATAAAAGTTGGGTTGCTCGCGTCGCTGAGGTGATAGATATTCCATTCTGTGTTGCA
GGTGGTATTAAAACCGCAGAAGATGCAGCAAAGATTTTAGAGTTTGGTGCAGACAAAGTATCAATTAACTCTCCTGCATT
GGCAAATCCAGAACTTATCACAGAACTTGCCGATAAATTTGGTGTTCAGTGTATCGTGGTAGGTATTGACTCTTATTTTG
ATAAAGATACTGGGAAATACCAAGTTTATCAGTTTACCGGTGATGAAGAGCGTACAAAAGCAACCAAATGGGAAACCAAA
GATTGGGTGCAAGAAGTACAGAAACGTGGTGCGGGCGAAATCGTACTAAATATGATGAATCAAGATGGTGTTCGTAATGG
TTACGATCTTGAGCAATTGAATATGGTGCGAGAAGTGTGTTATGTGCCACTCATTGCATCTGGTGGTGCTGGTGAAATGG
TTCACTTTGCAGATGCCTATAAAAAAACAAACGTAGACGGTGCATTAGCGGCATCGGTATTTCACAAACAAATCATCAAT
ATTGGTGAATTAAAAGACTATTTAGCCGAACAGGATGTAGAGGTACGCCGATGA

Upstream 100 bases:

>100_bases
CATTAGATGATATCGCTGCATTGAAAGGCTCTGGTGTTGCTGGTGTGATTGTGGGTCGAGCTCTGCTTGATGGAAAATTT
ACCGCAGAGGAGGCATTCCA

Downstream 100 bases:

>100_bases
GCCAACAAGAATTAGCAACACGTATTGATTGGGAAAAAGTGGATGGTTTAGTACCTGCAATTATTCAAGATTTTGGTTCA
AGCCAAGTGTTGATGATGGG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF1; ImGP synthase subunit hisF1; IGPS subunit hisF1 [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIKTAEDAAKILEFGADKVSINSPALANPELITELADKFGVQCIVVGIDSYFDKDTGKYQVYQFTGDEERTKATKWETK
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLNMVREVCYVPLIASGGAGEMVHFADAYKKTNVDGALAASVFHKQIIN
IGELKDYLAEQDVEVRR

Sequences:

>Translated_257_residues
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIKTAEDAAKILEFGADKVSINSPALANPELITELADKFGVQCIVVGIDSYFDKDTGKYQVYQFTGDEERTKATKWETK
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLNMVREVCYVPLIASGGAGEMVHFADAYKKTNVDGALAASVFHKQIIN
IGELKDYLAEQDVEVRR
>Mature_257_residues
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRVVDKSWVARVAEVIDIPFCVA
GGIKTAEDAAKILEFGADKVSINSPALANPELITELADKFGVQCIVVGIDSYFDKDTGKYQVYQFTGDEERTKATKWETK
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLNMVREVCYVPLIASGGAGEMVHFADAYKKTNVDGALAASVFHKQIIN
IGELKDYLAEQDVEVRR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=84.375, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI87082028, Length=231, Percent_Identity=26.8398268398268, Blast_Score=73, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319725, Length=316, Percent_Identity=31.9620253164557, Blast_Score=133, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 28441; Mature: 28441

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVARVAEVIDIPFCVAGGIKTAEDAAKILEFGADKVSINSPALANPELITELADKF
ECHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDSYFDKDTGKYQVYQFTGDEERTKATKWETKDWVQEVQKRGAGEIVLNMMN
CCEEEEEECCHHCCCCCCCEEEEEECCCHHHHHHHCCCCHHHHHHHHHCCCCCEEHHHHC
QDGVRNGYDLEQLNMVREVCYVPLIASGGAGEMVHFADAYKKTNVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IGELKDYLAEQDVEVRR
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVARVAEVIDIPFCVAGGIKTAEDAAKILEFGADKVSINSPALANPELITELADKF
ECHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDSYFDKDTGKYQVYQFTGDEERTKATKWETKDWVQEVQKRGAGEIVLNMMN
CCEEEEEECCHHCCCCCCCEEEEEECCCHHHHHHHCCCCHHHHHHHHHCCCCCEEHHHHC
QDGVRNGYDLEQLNMVREVCYVPLIASGGAGEMVHFADAYKKTNVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IGELKDYLAEQDVEVRR
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA