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The map label for this gene is hisH

Identifier: 59711623

GI number: 59711623

Start: 1123444

End: 1124058

Strand: Direct

Name: hisH

Synonym: VF_1016

Alternate gene names: 59711623

Gene position: 1123444-1124058 (Clockwise)

Preceding gene: 59711622

Following gene: 59711624

Centisome position: 38.77

GC content: 43.09

Gene sequence:

>615_bases
ATGACAAATCAAAACGTCGTTATTATTGATACGGGTTGTTCTAACGTTTCATCAGTGAGATTTGCGATTGAGCGTTTGGG
TTACGAGGTTACGATAAGCCGAGATCCTCAAGTGGTTCTGGCTGCAGATAAGCTGTTTTTACCGGGTGTTGGCACGGCAA
GCGAAGCGATGAAAAACTTGGCTGAACGTGATCTCATTGAGTTGGTTAAACAAGTAGAAAAGCCATTATTGGGTATCTGC
TTAGGTATGCAGCTGCTGGGTAAACTTTCTGAAGAGAAAGGTCAAAAAGCCGATCAAATTGTGGATTGTTTAGGTTTGTG
TGACGGTGAAGTTCGTAAGCTTCAAACCGGTGATTTACCACTGCCTCACATGGGGTGGAATACCATTAAACCTGTTGAAG
GTAACCCGCTGTTTAAAGACATCCCAGAGAACAGTTATTTCTACTTTGTACACAGTTTTGGTATGCAAGTAGGCGACTAT
ACTATTGCTTCGTGTGATTACGGACAACCGTTCACCGCTGCACTGCAAGCAGGTAATTACTACGGTGTTCAGTTTCACCC
AGAGCGCTCAAGTAAAGTCGGTTCAAAGCTTATTCAGAACTTTTTGGAGCTATAA

Upstream 100 bases:

>100_bases
TTGAAAGCCTATTTAAAGCGTTTGGTCGTACGTTACGCCAAGCGATTAAAGTAGAAGGTAATGAACTGCCAAGCTCAAAA
GGCGTATTGTAGGAGTGACT

Downstream 100 bases:

>100_bases
GAGCAGGGACGAGGTGCGAGATCACTTCGCTTCGAGGTTTTAGGGTTTTTACTCGTCCCTTGGATCTCGCAATGAGCGAA
GCGAATGTTCTCGGTTCTGC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MTNQNVVIIDTGCSNVSSVRFAIERLGYEVTISRDPQVVLAADKLFLPGVGTASEAMKNLAERDLIELVKQVEKPLLGIC
LGMQLLGKLSEEKGQKADQIVDCLGLCDGEVRKLQTGDLPLPHMGWNTIKPVEGNPLFKDIPENSYFYFVHSFGMQVGDY
TIASCDYGQPFTAALQAGNYYGVQFHPERSSKVGSKLIQNFLEL

Sequences:

>Translated_204_residues
MTNQNVVIIDTGCSNVSSVRFAIERLGYEVTISRDPQVVLAADKLFLPGVGTASEAMKNLAERDLIELVKQVEKPLLGIC
LGMQLLGKLSEEKGQKADQIVDCLGLCDGEVRKLQTGDLPLPHMGWNTIKPVEGNPLFKDIPENSYFYFVHSFGMQVGDY
TIASCDYGQPFTAALQAGNYYGVQFHPERSSKVGSKLIQNFLEL
>Mature_203_residues
TNQNVVIIDTGCSNVSSVRFAIERLGYEVTISRDPQVVLAADKLFLPGVGTASEAMKNLAERDLIELVKQVEKPLLGICL
GMQLLGKLSEEKGQKADQIVDCLGLCDGEVRKLQTGDLPLPHMGWNTIKPVEGNPLFKDIPENSYFYFVHSFGMQVGDYT
IASCDYGQPFTAALQAGNYYGVQFHPERSSKVGSKLIQNFLEL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=200, Percent_Identity=59.5, Blast_Score=249, Evalue=8e-68,
Organism=Saccharomyces cerevisiae, GI6319725, Length=214, Percent_Identity=33.1775700934579, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_VIBF1 (Q5E635)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_204399.1
- ProteinModelPortal:   Q5E635
- SMR:   Q5E635
- STRING:   Q5E635
- GeneID:   3277541
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_1016
- NMPDR:   fig|312309.3.peg.865
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- PhylomeDB:   Q5E635
- ProtClustDB:   PRK13170
- BioCyc:   VFIS312309:VF1016-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.- [C]

Molecular weight: Translated: 22462; Mature: 22331

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 80-80 ACT_SITE 186-186 ACT_SITE 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQNVVIIDTGCSNVSSVRFAIERLGYEVTISRDPQVVLAADKLFLPGVGTASEAMKNL
CCCCCEEEEECCCCCHHHHHHHHHHCCEEEEECCCCCEEEEECEEECCCCCCHHHHHHHH
AERDLIELVKQVEKPLLGICLGMQLLGKLSEEKGQKADQIVDCLGLCDGEVRKLQTGDLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHEEECCCCC
LPHMGWNTIKPVEGNPLFKDIPENSYFYFVHSFGMQVGDYTIASCDYGQPFTAALQAGNY
CCCCCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCCCCEEEEECCCCCCHHHHHHCCCE
YGVQFHPERSSKVGSKLIQNFLEL
EEEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNQNVVIIDTGCSNVSSVRFAIERLGYEVTISRDPQVVLAADKLFLPGVGTASEAMKNL
CCCCEEEEECCCCCHHHHHHHHHHCCEEEEECCCCCEEEEECEEECCCCCCHHHHHHHH
AERDLIELVKQVEKPLLGICLGMQLLGKLSEEKGQKADQIVDCLGLCDGEVRKLQTGDLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHEEECCCCC
LPHMGWNTIKPVEGNPLFKDIPENSYFYFVHSFGMQVGDYTIASCDYGQPFTAALQAGNY
CCCCCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCCCCEEEEECCCCCCHHHHHHCCCE
YGVQFHPERSSKVGSKLIQNFLEL
EEEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA