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The map label for this gene is gshA2 [H]

Identifier: 59711580

GI number: 59711580

Start: 1066259

End: 1067857

Strand: Direct

Name: gshA2 [H]

Synonym: VF_0973

Alternate gene names: 59711580

Gene position: 1066259-1067857 (Clockwise)

Preceding gene: 59711579

Following gene: 59711581

Centisome position: 36.8

GC content: 36.71

Gene sequence:

>1599_bases
ATGAAAAATACAGATGTGAAATACTTAGAACCTCATATTCTTTCTTTTTTAAACAATGAACATGCCATGAATACACTGCA
TGACATTCGACGTGGAATTGAAAGAGAATTGATCCGTACGACGCCTGAAAGCCGCATTTCAAACTTACCTCATCCAGAAG
AGTTAGGCTCTGCTTTAACGCACCCTTACATCACAACTGATTTTGCGGAAGCACAATTAGAGTTGGTGACTCCTGCAATG
ACGGAAAGAAAGACGACGTTTGATTCGTTAGCTTCTTTACATCATTTTGTCGCTACTCGTCTGCCTGACAATGAAATCAT
GTGGGGAGCAAGTATGCCACCTGAGCTGCCAAGTGATGATGAGATTAAGATCGCAGCGTATGGAACATCAAATGCTGGAT
TACATAAGGTACGTTACCGTGAAGGATTGGCGAATCGTTATGGAAAAAGAATGCAACTAATTTCAGGTATTCATTATAAT
TTTTCATTACCAGATTCTTTTTGGGAAATCTTACATTCACATATTGGTAGTGAATTACTCTTAAATGATTTCATTTCAGA
ACGTTATTTTCATTTGATTCGTAATGTCCTACGTAATGGTTGGATTATTCCATATTTATTTGGTGCATCACCTGCGGTAG
ATAAAAGTTATTTAATCAATCAAGAACACGCTTTAAATCTTCTTGATGACGAGACTTATTATTTGCCATGGGCGACATCT
TTACGCTTGAGTAACATGGGATATAGCAGTAATGAGCAATCTTTTTATCCAACAAGCTTTAATAGTAAGCAAGAGTATTT
GGCTGATTTATGTCACGCATTAACGACACCAAGTGAACGCTATACTCATTTAAATGGAGATCAGCAACTGAATGCATCGG
TCTTACAATTAGAAAATGAGCTATACGGTTCTATAAGGCCTAAAATCGTTAGTGATCAATTACGTCCTTTGTATGCGATG
TGTCATCACGGTATTCAATATATTGAACTACGTAGTCTAGATAACAATCCACTATTACCATTAGGTATCAGTGAAGATCA
AAGCTATTTTTTAGATGCTTTTCTCACATATAACGCCTTAGCACCAAGCCCTGAATTAACAGATTCTGAACGAGAGTTAA
TTGCTCGTCGTCAAGAGTTAGTGGCAACAGAAGGGAGAAAAGAAGGGCTATTATTACCAACGCTACAAGGTGATAGAGAG
CTTAAAGAGCTTGGCTTGACTTTATTATCATCAATGATGCCAGTTGCGTCATGGTTAGATAATGTGTTTGCTACAGAAGG
ACATAAACAAGGCATTGAGCGTGAGCAGGTTAAGTTTGTAGATAGTAATTTAACGCCATCAGCGCAAATATTAACAATAA
TGAAAGATGAAAAACTGTCATATAAACACGTAACACAAAAATTATCTGAAATGCATTTTAAACAGCATAAAGATGTCGAA
ATTAATGAAGAAGAAATGGTAGGTTTATCCCGATTAGTTGGCGAGTCTTTAGCTAAACAGCAAAGAATGGAGACGTTACA
AGATATGAGTTTTGATGAATTTATTCAGAAAAAAAACGATTTGCAGTGTGATTGTGAGATAGAAGAGGTCGTAGCGTAA

Upstream 100 bases:

>100_bases
TAATTACTTTATCTTTTTTCATTTTATTGAAATTTTTTTGCGTCTTTTTCTTTATCTGTTCGTCTTCAAGTTGTTAGAAA
CATTTTTATGAAGAACATAA

Downstream 100 bases:

>100_bases
TTTAATGAATATTTTGTGGAAATTGCGCGGTTACATCAAGATGTTTTCACGCCAGTACATTATCGCCATCATTGCATTGC
AAGTGGTGGCTTTATTAAGT

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MKNTDVKYLEPHILSFLNNEHAMNTLHDIRRGIERELIRTTPESRISNLPHPEELGSALTHPYITTDFAEAQLELVTPAM
TERKTTFDSLASLHHFVATRLPDNEIMWGASMPPELPSDDEIKIAAYGTSNAGLHKVRYREGLANRYGKRMQLISGIHYN
FSLPDSFWEILHSHIGSELLLNDFISERYFHLIRNVLRNGWIIPYLFGASPAVDKSYLINQEHALNLLDDETYYLPWATS
LRLSNMGYSSNEQSFYPTSFNSKQEYLADLCHALTTPSERYTHLNGDQQLNASVLQLENELYGSIRPKIVSDQLRPLYAM
CHHGIQYIELRSLDNNPLLPLGISEDQSYFLDAFLTYNALAPSPELTDSERELIARRQELVATEGRKEGLLLPTLQGDRE
LKELGLTLLSSMMPVASWLDNVFATEGHKQGIEREQVKFVDSNLTPSAQILTIMKDEKLSYKHVTQKLSEMHFKQHKDVE
INEEEMVGLSRLVGESLAKQQRMETLQDMSFDEFIQKKNDLQCDCEIEEVVA

Sequences:

>Translated_532_residues
MKNTDVKYLEPHILSFLNNEHAMNTLHDIRRGIERELIRTTPESRISNLPHPEELGSALTHPYITTDFAEAQLELVTPAM
TERKTTFDSLASLHHFVATRLPDNEIMWGASMPPELPSDDEIKIAAYGTSNAGLHKVRYREGLANRYGKRMQLISGIHYN
FSLPDSFWEILHSHIGSELLLNDFISERYFHLIRNVLRNGWIIPYLFGASPAVDKSYLINQEHALNLLDDETYYLPWATS
LRLSNMGYSSNEQSFYPTSFNSKQEYLADLCHALTTPSERYTHLNGDQQLNASVLQLENELYGSIRPKIVSDQLRPLYAM
CHHGIQYIELRSLDNNPLLPLGISEDQSYFLDAFLTYNALAPSPELTDSERELIARRQELVATEGRKEGLLLPTLQGDRE
LKELGLTLLSSMMPVASWLDNVFATEGHKQGIEREQVKFVDSNLTPSAQILTIMKDEKLSYKHVTQKLSEMHFKQHKDVE
INEEEMVGLSRLVGESLAKQQRMETLQDMSFDEFIQKKNDLQCDCEIEEVVA
>Mature_532_residues
MKNTDVKYLEPHILSFLNNEHAMNTLHDIRRGIERELIRTTPESRISNLPHPEELGSALTHPYITTDFAEAQLELVTPAM
TERKTTFDSLASLHHFVATRLPDNEIMWGASMPPELPSDDEIKIAAYGTSNAGLHKVRYREGLANRYGKRMQLISGIHYN
FSLPDSFWEILHSHIGSELLLNDFISERYFHLIRNVLRNGWIIPYLFGASPAVDKSYLINQEHALNLLDDETYYLPWATS
LRLSNMGYSSNEQSFYPTSFNSKQEYLADLCHALTTPSERYTHLNGDQQLNASVLQLENELYGSIRPKIVSDQLRPLYAM
CHHGIQYIELRSLDNNPLLPLGISEDQSYFLDAFLTYNALAPSPELTDSERELIARRQELVATEGRKEGLLLPTLQGDRE
LKELGLTLLSSMMPVASWLDNVFATEGHKQGIEREQVKFVDSNLTPSAQILTIMKDEKLSYKHVTQKLSEMHFKQHKDVE
INEEEMVGLSRLVGESLAKQQRMETLQDMSFDEFIQKKNDLQCDCEIEEVVA

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=503, Percent_Identity=40.7554671968191, Blast_Score=337, Evalue=1e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 60932; Mature: 60932

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTDVKYLEPHILSFLNNEHAMNTLHDIRRGIERELIRTTPESRISNLPHPEELGSALT
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHC
HPYITTDFAEAQLELVTPAMTERKTTFDSLASLHHFVATRLPDNEIMWGASMPPELPSDD
CCCEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCC
EIKIAAYGTSNAGLHKVRYREGLANRYGKRMQLISGIHYNFSLPDSFWEILHSHIGSELL
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHH
LNDFISERYFHLIRNVLRNGWIIPYLFGASPAVDKSYLINQEHALNLLDDETYYLPWATS
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHCCHHHHHHEECCCCEEECCHHH
LRLSNMGYSSNEQSFYPTSFNSKQEYLADLCHALTTPSERYTHLNGDQQLNASVLQLENE
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHEECCCCCHHHHHHHHHHHH
LYGSIRPKIVSDQLRPLYAMCHHGIQYIELRSLDNNPLLPLGISEDQSYFLDAFLTYNAL
HHHCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHCC
APSPELTDSERELIARRQELVATEGRKEGLLLPTLQGDRELKELGLTLLSSMMPVASWLD
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
NVFATEGHKQGIEREQVKFVDSNLTPSAQILTIMKDEKLSYKHVTQKLSEMHFKQHKDVE
HHHHCCCHHCCCCHHHHHHHCCCCCCCHHEEEEECCCCCHHHHHHHHHHHHHHHHCCCCC
INEEEMVGLSRLVGESLAKQQRMETLQDMSFDEFIQKKNDLQCDCEIEEVVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHCC
>Mature Secondary Structure
MKNTDVKYLEPHILSFLNNEHAMNTLHDIRRGIERELIRTTPESRISNLPHPEELGSALT
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHC
HPYITTDFAEAQLELVTPAMTERKTTFDSLASLHHFVATRLPDNEIMWGASMPPELPSDD
CCCEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCC
EIKIAAYGTSNAGLHKVRYREGLANRYGKRMQLISGIHYNFSLPDSFWEILHSHIGSELL
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHH
LNDFISERYFHLIRNVLRNGWIIPYLFGASPAVDKSYLINQEHALNLLDDETYYLPWATS
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHCCHHHHHHEECCCCEEECCHHH
LRLSNMGYSSNEQSFYPTSFNSKQEYLADLCHALTTPSERYTHLNGDQQLNASVLQLENE
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHEECCCCCHHHHHHHHHHHH
LYGSIRPKIVSDQLRPLYAMCHHGIQYIELRSLDNNPLLPLGISEDQSYFLDAFLTYNAL
HHHCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEECCCCCHHHHHHHHHHHHCC
APSPELTDSERELIARRQELVATEGRKEGLLLPTLQGDRELKELGLTLLSSMMPVASWLD
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
NVFATEGHKQGIEREQVKFVDSNLTPSAQILTIMKDEKLSYKHVTQKLSEMHFKQHKDVE
HHHHCCCHHCCCCHHHHHHHCCCCCCCHHEEEEECCCCCHHHHHHHHHHHHHHHHCCCCC
INEEEMVGLSRLVGESLAKQQRMETLQDMSFDEFIQKKNDLQCDCEIEEVVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA