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The map label for this gene is pcl [H]

Identifier: 59711456

GI number: 59711456

Start: 934141

End: 935544

Strand: Direct

Name: pcl [H]

Synonym: VF_0849

Alternate gene names: 59711456

Gene position: 934141-935544 (Clockwise)

Preceding gene: 59711455

Following gene: 59711457

Centisome position: 32.24

GC content: 40.03

Gene sequence:

>1404_bases
ATGGTTAGTGCGCCAATGGATAAGAAAAAAAGAAGTATGAAAACAGTCTCTCTTTCGCAATTAGTATTACAAGATAAAAC
AGACCACACGGTGTGTTTTGATGAGCAGCACTCATATATTTCTTATTCTCAATTTCAAAAAGACGTTCAGAAAGCGTGTG
CCTTTTTTCAGCAGCAACCTCACACTAAGTGGGCATTGTGTTTTGATGACAGCTATTATTTTTCGGTTACTTTTCTTGCA
TTAGCCCATGCGGGTAAACACCTTATTCTTCTAGGTAATCATCAACCAGCGGCATTGGCCGAATTATCTCAGCATTTTGA
TGCCTTAATTCATGATGACATTATTAAAGTGATGCCATGCAGTGAGCATGTTGCCTATCATGATATAAAAAGTGACCATT
TAGCTACATTATCACCACTTCCTCTTCATGAGGTTGAGCTTACTTTATTTACTTCTGGCTCAACTGATACACCCAAGCCC
ATTGCTAAAAACCTTCAATTACTTGATAACGAAATTCAACAATTAGACTGTTTATGGGGTGAAAAACTCGGACAAAGTGA
CATTTACAGCACCGTATCACACCAACATATCTATGGTTTATTATTTAGAGTGTTGTGGCCATTAACGGCTGGTCGTGCCT
TTAGTCGCCATAACTTTATCTACCCAGAGCAGATTTTATCTCATCGACCAGATGATAAAAAAACGCTTATTTCTAGTCCC
GCATTGCTTAAGCGCTTGGTTGATCACGAAGGGTACAATGGCTATCAGATGGTTTTCTCTTCCGGCGGACCTCTTGCTAT
TGAAGGGGCTGAGCAAAGTGAGAAAAAACTCTCACTGCGTCCATTTGAAGTTTATGGTAGTACAGAAACAGGTGGTATTG
GCTACCGTCAGCAGTTTGAACCAAGCTCTTTATGGTGCTTCTTTCCTTCTCATCAAGCTCGTTTAAACATTGAACATTGC
TTAGAATTGCTTTCTCCATTTATTGATGACGAAGAGTGGTACGCGACGAGCGATTACTGTGATTTGCTTGCTAATGGTCA
GTTTGTTCTAAAAGGTCGCGTCGATCGCGTGATCAAAATTGAAGAAAAGCGCCTCTCTTTAACGGAAGTTGAAAAACGAT
TAATGCAGTTAGAGTGGATTAATGAAGCGGCAGTGATCCCGATGGAAGAAGAAAAACGCACCGTATTGGGCGCAGTGATT
GTTCTATCAGAAGCAGGAACTAAACTAATGGAGCAGGGCTCTAAGGGTAAGTTTTGGTTGAGATTACGCTCAGAATTACG
TGAATGGTTAGAGCCTGTTGGTATTCCTCGTCGTTTTAGAACTGTTAGTGAAATTCCATTAAATAGCCAAGGTAAACGTC
AAATGCACGATTTAAAGCAGCTATTTAATGATGCGAATAATTAA

Upstream 100 bases:

>100_bases
GCTCTATCTCTTTTTCTACCTGTTTCATGGATATGAAGATGTGGATGCTATACAACGGATTTATTAGCTACTTGCTTATT
GGAACTTTGTTCATAAGCGA

Downstream 100 bases:

>100_bases
AAAAGATTAGATTATGATAAAACGTAAGCCAAGTATCCTTACAACAAAAGATATCTCAGTAAATGAAGCGCAACCAAGTG
TGCTTATTTGTATGCGTGTG

Product: AMP-(fatty)acid ligase

Products: NA

Alternate protein names: 4CL; 4-coumaroyl-CoA synthase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MVSAPMDKKKRSMKTVSLSQLVLQDKTDHTVCFDEQHSYISYSQFQKDVQKACAFFQQQPHTKWALCFDDSYYFSVTFLA
LAHAGKHLILLGNHQPAALAELSQHFDALIHDDIIKVMPCSEHVAYHDIKSDHLATLSPLPLHEVELTLFTSGSTDTPKP
IAKNLQLLDNEIQQLDCLWGEKLGQSDIYSTVSHQHIYGLLFRVLWPLTAGRAFSRHNFIYPEQILSHRPDDKKTLISSP
ALLKRLVDHEGYNGYQMVFSSGGPLAIEGAEQSEKKLSLRPFEVYGSTETGGIGYRQQFEPSSLWCFFPSHQARLNIEHC
LELLSPFIDDEEWYATSDYCDLLANGQFVLKGRVDRVIKIEEKRLSLTEVEKRLMQLEWINEAAVIPMEEEKRTVLGAVI
VLSEAGTKLMEQGSKGKFWLRLRSELREWLEPVGIPRRFRTVSEIPLNSQGKRQMHDLKQLFNDANN

Sequences:

>Translated_467_residues
MVSAPMDKKKRSMKTVSLSQLVLQDKTDHTVCFDEQHSYISYSQFQKDVQKACAFFQQQPHTKWALCFDDSYYFSVTFLA
LAHAGKHLILLGNHQPAALAELSQHFDALIHDDIIKVMPCSEHVAYHDIKSDHLATLSPLPLHEVELTLFTSGSTDTPKP
IAKNLQLLDNEIQQLDCLWGEKLGQSDIYSTVSHQHIYGLLFRVLWPLTAGRAFSRHNFIYPEQILSHRPDDKKTLISSP
ALLKRLVDHEGYNGYQMVFSSGGPLAIEGAEQSEKKLSLRPFEVYGSTETGGIGYRQQFEPSSLWCFFPSHQARLNIEHC
LELLSPFIDDEEWYATSDYCDLLANGQFVLKGRVDRVIKIEEKRLSLTEVEKRLMQLEWINEAAVIPMEEEKRTVLGAVI
VLSEAGTKLMEQGSKGKFWLRLRSELREWLEPVGIPRRFRTVSEIPLNSQGKRQMHDLKQLFNDANN
>Mature_467_residues
MVSAPMDKKKRSMKTVSLSQLVLQDKTDHTVCFDEQHSYISYSQFQKDVQKACAFFQQQPHTKWALCFDDSYYFSVTFLA
LAHAGKHLILLGNHQPAALAELSQHFDALIHDDIIKVMPCSEHVAYHDIKSDHLATLSPLPLHEVELTLFTSGSTDTPKP
IAKNLQLLDNEIQQLDCLWGEKLGQSDIYSTVSHQHIYGLLFRVLWPLTAGRAFSRHNFIYPEQILSHRPDDKKTLISSP
ALLKRLVDHEGYNGYQMVFSSGGPLAIEGAEQSEKKLSLRPFEVYGSTETGGIGYRQQFEPSSLWCFFPSHQARLNIEHC
LELLSPFIDDEEWYATSDYCDLLANGQFVLKGRVDRVIKIEEKRLSLTEVEKRLMQLEWINEAAVIPMEEEKRTVLGAVI
VLSEAGTKLMEQGSKGKFWLRLRSELREWLEPVGIPRRFRTVSEIPLNSQGKRQMHDLKQLFNDANN

Specific function: Converts p-coumaric acid into p-coumaryl CoA. This is necessary for the activation of the photoactive yellow protein (PYP) chromophore [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012743
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.12 [H]

Molecular weight: Translated: 53433; Mature: 53433

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSAPMDKKKRSMKTVSLSQLVLQDKTDHTVCFDEQHSYISYSQFQKDVQKACAFFQQQP
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
HTKWALCFDDSYYFSVTFLALAHAGKHLILLGNHQPAALAELSQHFDALIHDDIIKVMPC
CCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
SEHVAYHDIKSDHLATLSPLPLHEVELTLFTSGSTDTPKPIAKNLQLLDNEIQQLDCLWG
CCHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EKLGQSDIYSTVSHQHIYGLLFRVLWPLTAGRAFSRHNFIYPEQILSHRPDDKKTLISSP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCHHHHHCCH
ALLKRLVDHEGYNGYQMVFSSGGPLAIEGAEQSEKKLSLRPFEVYGSTETGGIGYRQQFE
HHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCHHCCCCCEEEECCCCCCCCCCCCCCC
PSSLWCFFPSHQARLNIEHCLELLSPFIDDEEWYATSDYCDLLANGQFVLKGRVDRVIKI
CCCEEEECCCCCCEECHHHHHHHHHHHCCCCCCEECHHHHHHHCCCCEEEECCCCCEEEE
EEKRLSLTEVEKRLMQLEWINEAAVIPMEEEKRTVLGAVIVLSEAGTKLMEQGSKGKFWL
HHHHCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHH
RLRSELREWLEPVGIPRRFRTVSEIPLNSQGKRQMHDLKQLFNDANN
HHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVSAPMDKKKRSMKTVSLSQLVLQDKTDHTVCFDEQHSYISYSQFQKDVQKACAFFQQQP
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
HTKWALCFDDSYYFSVTFLALAHAGKHLILLGNHQPAALAELSQHFDALIHDDIIKVMPC
CCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
SEHVAYHDIKSDHLATLSPLPLHEVELTLFTSGSTDTPKPIAKNLQLLDNEIQQLDCLWG
CCHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EKLGQSDIYSTVSHQHIYGLLFRVLWPLTAGRAFSRHNFIYPEQILSHRPDDKKTLISSP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCHHHHHCCH
ALLKRLVDHEGYNGYQMVFSSGGPLAIEGAEQSEKKLSLRPFEVYGSTETGGIGYRQQFE
HHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCHHCCCCCEEEECCCCCCCCCCCCCCC
PSSLWCFFPSHQARLNIEHCLELLSPFIDDEEWYATSDYCDLLANGQFVLKGRVDRVIKI
CCCEEEECCCCCCEECHHHHHHHHHHHCCCCCCEECHHHHHHHCCCCEEEECCCCCEEEE
EEKRLSLTEVEKRLMQLEWINEAAVIPMEEEKRTVLGAVIVLSEAGTKLMEQGSKGKFWL
HHHHCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHH
RLRSELREWLEPVGIPRRFRTVSEIPLNSQGKRQMHDLKQLFNDANN
HHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9630474 [H]