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The map label for this gene is dsbA [H]

Identifier: 59711253

GI number: 59711253

Start: 706098

End: 706697

Strand: Direct

Name: dsbA [H]

Synonym: VF_0646

Alternate gene names: 59711253

Gene position: 706098-706697 (Clockwise)

Preceding gene: 172087671

Following gene: 59711256

Centisome position: 24.37

GC content: 37.33

Gene sequence:

>600_bases
ATGAAAAAGTTATTTGTTTTTTTATCAGCAGTTATGTTTGCATTCTCTGTTCAGGCAACACAGTTTGAGGAGGGGAAATA
TTATCAAGTTCTCGATGTAGCGAAATCAACCCAACCCAAGGTGACGGAGTTTTTTTCATTCTATTGTCCGCACTGCTATA
AGTTTGAAGCGGTGATTAAGAATTTAAAACCCGCTTTAGCGAAGAGCGCCAAATTTGAAAAAGTGCATGTTGCTTTTATG
GGAAATGATATGGCAGTACCTATGGCAAAATCGTACGCCACTATGGTTGTACTTGGGGTTGAAGATAAAATGGTACCAGC
TATGTTTAAGCAGATTCATGAATTAGGGCAACGTCCAAAAAATGAAGATGAACTTCGTCAAGTCTTTATCAATAATGGTA
TTGACCCTGATAAATATGATGAGGCTTATAATAGTGCGGCTGTGAATGCGATGCAGCGTAAGTTTGATATTCAGTTTGAA
GCAAGTACGTTAACAGGGGTTCCAGGAGTATTGGTGAACAATAAATACATTGTTAAACCTAATGCAATTCGTAGTTACGA
CGAATACAACCAACTGGTAAATTATTTACTGACATTGTAA

Upstream 100 bases:

>100_bases
CGTACGTAATCAAACCTTATTTCGCCCTCCAATTGTGTAATTCTTAATGTAGAGTTTTTGTATTTTTACCGATGTTATTA
ATCGATTTAGGATTTATTCA

Downstream 100 bases:

>100_bases
TTTTTTATTTAAGCTACAAACTTGAGATACAAAAAAGCCCTCGCAGATTGAGGGCTTTTTATTGCATGACATTATGCAGG
GATATTTTGTGTGAACACTT

Product: periplasmic protein disulfide isomerase, DsbA-like protein

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MKKLFVFLSAVMFAFSVQATQFEEGKYYQVLDVAKSTQPKVTEFFSFYCPHCYKFEAVIKNLKPALAKSAKFEKVHVAFM
GNDMAVPMAKSYATMVVLGVEDKMVPAMFKQIHELGQRPKNEDELRQVFINNGIDPDKYDEAYNSAAVNAMQRKFDIQFE
ASTLTGVPGVLVNNKYIVKPNAIRSYDEYNQLVNYLLTL

Sequences:

>Translated_199_residues
MKKLFVFLSAVMFAFSVQATQFEEGKYYQVLDVAKSTQPKVTEFFSFYCPHCYKFEAVIKNLKPALAKSAKFEKVHVAFM
GNDMAVPMAKSYATMVVLGVEDKMVPAMFKQIHELGQRPKNEDELRQVFINNGIDPDKYDEAYNSAAVNAMQRKFDIQFE
ASTLTGVPGVLVNNKYIVKPNAIRSYDEYNQLVNYLLTL
>Mature_199_residues
MKKLFVFLSAVMFAFSVQATQFEEGKYYQVLDVAKSTQPKVTEFFSFYCPHCYKFEAVIKNLKPALAKSAKFEKVHVAFM
GNDMAVPMAKSYATMVVLGVEDKMVPAMFKQIHELGQRPKNEDELRQVFINNGIDPDKYDEAYNSAAVNAMQRKFDIQFE
ASTLTGVPGVLVNNKYIVKPNAIRSYDEYNQLVNYLLTL

Specific function: Involved in disulfide-bond formation. Required for the functional maturation of secreted virulence factors. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=205, Percent_Identity=36.5853658536585, Blast_Score=144, Evalue=4e-36,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 22689; Mature: 22689

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLFVFLSAVMFAFSVQATQFEEGKYYQVLDVAKSTQPKVTEFFSFYCPHCYKFEAVIK
CHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEHHCCCCCHHHHHHHHHCCCHHHHHHHHH
NLKPALAKSAKFEKVHVAFMGNDMAVPMAKSYATMVVLGVEDKMVPAMFKQIHELGQRPK
HHHHHHHHCCCCCEEEEEEECCCCCCCHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCCC
NEDELRQVFINNGIDPDKYDEAYNSAAVNAMQRKFDIQFEASTLTGVPGVLVNNKYIVKP
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCEEECCEEEECC
NAIRSYDEYNQLVNYLLTL
CHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLFVFLSAVMFAFSVQATQFEEGKYYQVLDVAKSTQPKVTEFFSFYCPHCYKFEAVIK
CHHHHHHHHHHHHHHHHHHHEECCCCEEEEEEHHCCCCCHHHHHHHHHCCCHHHHHHHHH
NLKPALAKSAKFEKVHVAFMGNDMAVPMAKSYATMVVLGVEDKMVPAMFKQIHELGQRPK
HHHHHHHHCCCCCEEEEEEECCCCCCCHHHCCEEEEEECCCCHHHHHHHHHHHHHHCCCC
NEDELRQVFINNGIDPDKYDEAYNSAAVNAMQRKFDIQFEASTLTGVPGVLVNNKYIVKP
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCEEECCEEEECC
NAIRSYDEYNQLVNYLLTL
CHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1631111; 1324389; 10952301; 9149147 [H]