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The map label for this gene is pepB [H]

Identifier: 59711231

GI number: 59711231

Start: 679973

End: 681259

Strand: Direct

Name: pepB [H]

Synonym: VF_0624

Alternate gene names: 59711231

Gene position: 679973-681259 (Clockwise)

Preceding gene: 59711230

Following gene: 59711232

Centisome position: 23.47

GC content: 42.11

Gene sequence:

>1287_bases
ATGTCACAAAAGATGACTGTACAACTTTCATCAACCGCAGCCCCTGCCCATTGGGGGGACAAAGCTATATTGTCTTTTAC
TTCTGAAGCTGCCGTTATTCATTGTATTGAAAGTGACGTATACAGTCTTATCCAGCGAGCGGCTCGTAAATTAAATTCAC
AAGGTTTAACCGCTGTTGAACTTGAGGGTGTCGACTGGGACCTTGAATCTATTTGGTCATTTATTCAAGGTTTCCGTGAC
TCTAAAAATAGCGATGATGTGAGTTGGACTGCATTAAGTGAAAATGATGAAAAAGAGTTAAACGCTCGAATTCTAACAAC
GAATTGGACTCGTCAAATCATTAATAAAACGGCTGAAGAAGTCGCTCCTCGTCAACTTGCTACTATGGCCGCAGAGTTTA
TTAAATCCGTTGCTCCAGAAGGCACTGTCACAACTAAGATTGTAAAAGATAAAGATCTTCTAACTCAAGGTTGGACGGGG
ATTTATGCCGTAGGCCGTGGCTCAGAACGTACCTCTGCAATGTTACAGTTAGACTATAACCCTACTGGAGACGAAAATGC
GCCAGTATTTGCTTGTCTAGTAGGTAAAGGCATTACGTTTGATTCTGGTGGTTACAGTATTAAACCATCAGCAGGTATGG
CATCGATGAAAGCGGATATGGGAGGATCTGCATTAGTGACAGCAGGTCTTGCGATGTCGATTCTTCGTGGTTTAGATAAG
CGTGTTAAGCTGATTCTATGTTGTGCTGAAAATATGATTTCAGGCCGAGCATTAAAGTTAGGTGATATCATCACTTATAA
AAATGGTAAGACAGTAGAAATTCTAAATACGGATGCAGAAGGACGTTTAGTTTTGGCTGATGGTCTTCAGTTTGCATCAG
AGCAAAAACCTGAACTTATAATTGACTGTGCGACGCTAACGGGTGCAGCGAAAATGGCTGTAGGTAATGATTTCCATTCA
CTATTAAGTTTTGATGAAGCATTGAGCCAAAAAGCGTTAAATGCGGCTCAAGAAGAGAATGAAGGTCTATGGCGTTTACC
ATTGATGGAATTCCACCGAGCTATGCTGCCATCAAACTTTGCTGATTTAGCGAATATTGGTTCTGGCCCATATTCTCCGG
GTGCAAGTACTGCCGCAGGCTTTTTATCTTACTTTGTTGAAGATTATCAGAAAGGATGGATTCATATGGACTGCTCAGCG
ACGTATCGCACAGCGCCATCTGATAAGTGGGCAGCCGGTGCGACCGGAGTGGGTGTAAGAACACTTGCTAATATCTTAAC
TAAATAA

Upstream 100 bases:

>100_bases
GTTTTATTCATTTATAACCGGTCGACGTTCACGCAATATAAGTAATCATATGCAATTAAATGATGTTATGGAGTTATTGC
GTATTTAAAGGAGATACACC

Downstream 100 bases:

>100_bases
TTAAAATGAAGATCGAGCTCGCTCGGTCTTCTTTTTTAACAGCTAATTCCTATCAGTATTTATACTAATTGTAAGTAAAT
AATAACTGGTCATCCTAGCT

Product: aminopeptidase B

Products: NA

Alternate protein names: Aminopeptidase B [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSQKMTVQLSSTAAPAHWGDKAILSFTSEAAVIHCIESDVYSLIQRAARKLNSQGLTAVELEGVDWDLESIWSFIQGFRD
SKNSDDVSWTALSENDEKELNARILTTNWTRQIINKTAEEVAPRQLATMAAEFIKSVAPEGTVTTKIVKDKDLLTQGWTG
IYAVGRGSERTSAMLQLDYNPTGDENAPVFACLVGKGITFDSGGYSIKPSAGMASMKADMGGSALVTAGLAMSILRGLDK
RVKLILCCAENMISGRALKLGDIITYKNGKTVEILNTDAEGRLVLADGLQFASEQKPELIIDCATLTGAAKMAVGNDFHS
LLSFDEALSQKALNAAQEENEGLWRLPLMEFHRAMLPSNFADLANIGSGPYSPGASTAAGFLSYFVEDYQKGWIHMDCSA
TYRTAPSDKWAAGATGVGVRTLANILTK

Sequences:

>Translated_428_residues
MSQKMTVQLSSTAAPAHWGDKAILSFTSEAAVIHCIESDVYSLIQRAARKLNSQGLTAVELEGVDWDLESIWSFIQGFRD
SKNSDDVSWTALSENDEKELNARILTTNWTRQIINKTAEEVAPRQLATMAAEFIKSVAPEGTVTTKIVKDKDLLTQGWTG
IYAVGRGSERTSAMLQLDYNPTGDENAPVFACLVGKGITFDSGGYSIKPSAGMASMKADMGGSALVTAGLAMSILRGLDK
RVKLILCCAENMISGRALKLGDIITYKNGKTVEILNTDAEGRLVLADGLQFASEQKPELIIDCATLTGAAKMAVGNDFHS
LLSFDEALSQKALNAAQEENEGLWRLPLMEFHRAMLPSNFADLANIGSGPYSPGASTAAGFLSYFVEDYQKGWIHMDCSA
TYRTAPSDKWAAGATGVGVRTLANILTK
>Mature_427_residues
SQKMTVQLSSTAAPAHWGDKAILSFTSEAAVIHCIESDVYSLIQRAARKLNSQGLTAVELEGVDWDLESIWSFIQGFRDS
KNSDDVSWTALSENDEKELNARILTTNWTRQIINKTAEEVAPRQLATMAAEFIKSVAPEGTVTTKIVKDKDLLTQGWTGI
YAVGRGSERTSAMLQLDYNPTGDENAPVFACLVGKGITFDSGGYSIKPSAGMASMKADMGGSALVTAGLAMSILRGLDKR
VKLILCCAENMISGRALKLGDIITYKNGKTVEILNTDAEGRLVLADGLQFASEQKPELIIDCATLTGAAKMAVGNDFHSL
LSFDEALSQKALNAAQEENEGLWRLPLMEFHRAMLPSNFADLANIGSGPYSPGASTAAGFLSYFVEDYQKGWIHMDCSAT
YRTAPSDKWAAGATGVGVRTLANILTK

Specific function: Probably plays an important role in intracellular peptide degradation [H]

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family [H]

Homologues:

Organism=Homo sapiens, GI41393561, Length=324, Percent_Identity=35.8024691358025, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI47155554, Length=296, Percent_Identity=34.7972972972973, Blast_Score=143, Evalue=3e-34,
Organism=Escherichia coli, GI87082123, Length=427, Percent_Identity=59.0163934426229, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI1790710, Length=303, Percent_Identity=41.9141914191419, Blast_Score=201, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI17556903, Length=358, Percent_Identity=31.2849162011173, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17565172, Length=228, Percent_Identity=29.3859649122807, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI221379063, Length=340, Percent_Identity=32.0588235294118, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI221379062, Length=340, Percent_Identity=32.0588235294118, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21357381, Length=340, Percent_Identity=32.0588235294118, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI20129969, Length=372, Percent_Identity=28.7634408602151, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI21355725, Length=334, Percent_Identity=30.2395209580838, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24661038, Length=377, Percent_Identity=28.9124668435013, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24662227, Length=403, Percent_Identity=27.0471464019851, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161077148, Length=372, Percent_Identity=26.8817204301075, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI20130057, Length=372, Percent_Identity=26.8817204301075, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21355645, Length=405, Percent_Identity=24.9382716049383, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24662223, Length=405, Percent_Identity=24.9382716049383, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI19922386, Length=372, Percent_Identity=26.6129032258064, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI20129963, Length=365, Percent_Identity=26.3013698630137, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24646701, Length=273, Percent_Identity=28.2051282051282, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646703, Length=273, Percent_Identity=28.2051282051282, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21358201, Length=273, Percent_Identity=28.2051282051282, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008330
- InterPro:   IPR011356
- InterPro:   IPR000819 [H]

Pfam domain/function: PF00883 Peptidase_M17 [H]

EC number: =3.4.11.23 [H]

Molecular weight: Translated: 46103; Mature: 45972

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00631 CYTOSOL_AP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKMTVQLSSTAAPAHWGDKAILSFTSEAAVIHCIESDVYSLIQRAARKLNSQGLTAVE
CCCEEEEEEECCCCCCCCCCHHHEEECCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEE
LEGVDWDLESIWSFIQGFRDSKNSDDVSWTALSENDEKELNARILTTNWTRQIINKTAEE
ECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHH
VAPRQLATMAAEFIKSVAPEGTVTTKIVKDKDLLTQGWTGIYAVGRGSERTSAMLQLDYN
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCEEEEECCCCCCCEEEEEEECC
PTGDENAPVFACLVGKGITFDSGGYSIKPSAGMASMKADMGGSALVTAGLAMSILRGLDK
CCCCCCCCEEEEEECCCEEECCCCCEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHH
RVKLILCCAENMISGRALKLGDIITYKNGKTVEILNTDAEGRLVLADGLQFASEQKPELI
HHEEHEEEHHHHHCCCEEEECCEEEECCCCEEEEEECCCCCCEEEECCCHHHCCCCCCEE
IDCATLTGAAKMAVGNDFHSLLSFDEALSQKALNAAQEENEGLWRLPLMEFHRAMLPSNF
EEEEHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHCCCCH
ADLANIGSGPYSPGASTAAGFLSYFVEDYQKGWIHMDCSATYRTAPSDKWAAGATGVGVR
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCHHH
TLANILTK
HHHHHHCC
>Mature Secondary Structure 
SQKMTVQLSSTAAPAHWGDKAILSFTSEAAVIHCIESDVYSLIQRAARKLNSQGLTAVE
CCEEEEEEECCCCCCCCCCHHHEEECCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEE
LEGVDWDLESIWSFIQGFRDSKNSDDVSWTALSENDEKELNARILTTNWTRQIINKTAEE
ECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHH
VAPRQLATMAAEFIKSVAPEGTVTTKIVKDKDLLTQGWTGIYAVGRGSERTSAMLQLDYN
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCEEEEECCCCCCCEEEEEEECC
PTGDENAPVFACLVGKGITFDSGGYSIKPSAGMASMKADMGGSALVTAGLAMSILRGLDK
CCCCCCCCEEEEEECCCEEECCCCCEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHH
RVKLILCCAENMISGRALKLGDIITYKNGKTVEILNTDAEGRLVLADGLQFASEQKPELI
HHEEHEEEHHHHHCCCEEEECCEEEECCCCEEEEEECCCCCCEEEECCCHHHCCCCCCEE
IDCATLTGAAKMAVGNDFHSLLSFDEALSQKALNAAQEENEGLWRLPLMEFHRAMLPSNF
EEEEHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHCCCCH
ADLANIGSGPYSPGASTAAGFLSYFVEDYQKGWIHMDCSATYRTAPSDKWAAGATGVGVR
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCHHH
TLANILTK
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA