Click here to switch to the map view.

The map label for this gene is trmJ [H]

Identifier: 59711222

GI number: 59711222

Start: 673207

End: 673932

Strand: Direct

Name: trmJ [H]

Synonym: VF_0615

Alternate gene names: 59711222

Gene position: 673207-673932 (Clockwise)

Preceding gene: 59711220

Following gene: 59711223

Centisome position: 23.23

GC content: 40.22

Gene sequence:

>726_bases
ATGTTAGATAAAATTCGTATTGTTCTTGTAGGAACTAGCCATTCAGGAAATATTGGTTCAGCTGCTCGCGCGATGAAAGT
GATGGGGTTAACTAATATGGTACTTGTTGCACCTGAGTGTGAAGTTGATGGCCAAGCCATTGCTTTAGCTGCAGGAGCAA
GTGACATAGCGAATAATGCACGTGTTGTAGATACATTAGATGAAGCGATTTCTGATTGTGGTCTTGTTATTGGTTCAAGT
GCTCGCTCTAGAACATTAGAATGGCCAATGTTAGAGCCACGAGAAGCGGGTATTAAAGCTATTGAAGAAGCGCCAACGCA
TCCAATTGCTTTTGTATTTGGTCGTGAAAGAGTCGGTTTGACAAATGAAGAGTTACAAAAATGTCATTTCCATGTATGTA
TTCCAGCTAACCCTGAATATAGCTCTTTAAATTTAGCAATGGCAGTGCAAACTATTAGTTACGAAGCACGCGTTGCATGG
TTAGACAGTCAAGCATATAAAGGGGAATCAAAAGAAGTTGATTACCCTTTGAACCATGAACTGGAATTATTCTACGAACA
CCTTGAAAAAGTGATGACGAATACCGACTTTATTAATAAGGCGCATCCAGGTCAGGTAATGAATAAAATGCGTCGTATGT
TTAACCGCACACGTCCAGAAACTCAAGAATTACGCATTTTACGCGGTGTATTAACCGCCGTAGAACGCAGTATGAAAGAC
AAGTAA

Upstream 100 bases:

>100_bases
AAAAAAGCGATCTAAGGTTAAAATCTCAGCGAGATAAAGAAGAAAGCCGTATTGCTTTATGTTAGTATCCGGCGGCTCTT
TTCATTAGATGGGAAATAAC

Downstream 100 bases:

>100_bases
TATCAAATAGTAAGTACTTGACTAAAATAGTAGGATAAATACTTGACCAAAATAGTCAGGTATGGGAAACTTCATTCCAT
ACGAATAGTGTGGATATGAG

Product: methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MLDKIRIVLVGTSHSGNIGSAARAMKVMGLTNMVLVAPECEVDGQAIALAAGASDIANNARVVDTLDEAISDCGLVIGSS
ARSRTLEWPMLEPREAGIKAIEEAPTHPIAFVFGRERVGLTNEELQKCHFHVCIPANPEYSSLNLAMAVQTISYEARVAW
LDSQAYKGESKEVDYPLNHELELFYEHLEKVMTNTDFINKAHPGQVMNKMRRMFNRTRPETQELRILRGVLTAVERSMKD
K

Sequences:

>Translated_241_residues
MLDKIRIVLVGTSHSGNIGSAARAMKVMGLTNMVLVAPECEVDGQAIALAAGASDIANNARVVDTLDEAISDCGLVIGSS
ARSRTLEWPMLEPREAGIKAIEEAPTHPIAFVFGRERVGLTNEELQKCHFHVCIPANPEYSSLNLAMAVQTISYEARVAW
LDSQAYKGESKEVDYPLNHELELFYEHLEKVMTNTDFINKAHPGQVMNKMRRMFNRTRPETQELRILRGVLTAVERSMKD
K
>Mature_241_residues
MLDKIRIVLVGTSHSGNIGSAARAMKVMGLTNMVLVAPECEVDGQAIALAAGASDIANNARVVDTLDEAISDCGLVIGSS
ARSRTLEWPMLEPREAGIKAIEEAPTHPIAFVFGRERVGLTNEELQKCHFHVCIPANPEYSSLNLAMAVQTISYEARVAW
LDSQAYKGESKEVDYPLNHELELFYEHLEKVMTNTDFINKAHPGQVMNKMRRMFNRTRPETQELRILRGVLTAVERSMKD
K

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=241, Percent_Identity=65.1452282157676, Blast_Score=328, Evalue=1e-91,
Organism=Escherichia coli, GI1790865, Length=154, Percent_Identity=36.3636363636364, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26767; Mature: 26767

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDKIRIVLVGTSHSGNIGSAARAMKVMGLTNMVLVAPECEVDGQAIALAAGASDIANNA
CCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCHHHCCCC
RVVDTLDEAISDCGLVIGSSARSRTLEWPMLEPREAGIKAIEEAPTHPIAFVFGRERVGL
CHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCC
TNEELQKCHFHVCIPANPEYSSLNLAMAVQTISYEARVAWLDSQAYKGESKEVDYPLNHE
CHHHHHHCEEEEEECCCCCCCCCEEEEEEEHHCCEEEEEEECCHHCCCCCCCCCCCCCCH
LELFYEHLEKVMTNTDFINKAHPGQVMNKMRRMFNRTRPETQELRILRGVLTAVERSMKD
HHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
K
C
>Mature Secondary Structure
MLDKIRIVLVGTSHSGNIGSAARAMKVMGLTNMVLVAPECEVDGQAIALAAGASDIANNA
CCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCHHHCCCC
RVVDTLDEAISDCGLVIGSSARSRTLEWPMLEPREAGIKAIEEAPTHPIAFVFGRERVGL
CHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCC
TNEELQKCHFHVCIPANPEYSSLNLAMAVQTISYEARVAWLDSQAYKGESKEVDYPLNHE
CHHHHHHCEEEEEECCCCCCCCCEEEEEEEHHCCEEEEEEECCHHCCCCCCCCCCCCCCH
LELFYEHLEKVMTNTDFINKAHPGQVMNKMRRMFNRTRPETQELRILRGVLTAVERSMKD
HHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA