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The map label for this gene is truC [H]

Identifier: 59711220

GI number: 59711220

Start: 671351

End: 672130

Strand: Direct

Name: truC [H]

Synonym: VF_0613

Alternate gene names: 59711220

Gene position: 671351-672130 (Clockwise)

Preceding gene: 59711219

Following gene: 59711222

Centisome position: 23.17

GC content: 38.72

Gene sequence:

>780_bases
GTGAATACAGAAGAAATGACAGCTGTGAATGATGAGTTATTAGAAGAACAAGCTGAGCCAATAGAGCTTGAGATTGTTTA
TCGTGATGAACATTTAATTGCGATTAATAAACCTGCAGGAATGTTAGTGCACCGTTCATGGCTTGATAAACACGAAACGG
TATTTGCGATGCAAACATTACGTGATCAAATTGGTCAGCATGTGTTTCCATTACATCGTTTAGACAGACCAACATCAGGG
ATATTATTCTTTGCTTTATCAAGCGAGATTGCTGCACAAGTATCTCCACTTTTCGCAAGCAGAGAAATAAAGAAAACCTA
TCATGCGATTGTTCGCGGATGGATTGAAGAGGCGAACACGCTTGATTATCCATTGAAAAAAGAACTCGATAAAATTGCAG
ACAAAAAGGCATCACAAGAAGTCGAAGCTAAAGAAGCGATTACAGAGTACAAACCGTTAGCTAAAGTGGAGTTACCTTAT
TCAACAGGTAAGTTTCCAACCACGCGGTATTGTTTGATGGAGTTAAAACCACATACTGGTAGAAAGCATCAACTTCGTCG
TCATATGGCACATTTACGTCACCCAATTGTTGGTGATACAAGTCACGGCGATGGAAAGCATAATCGTTTATTTAAAGAAA
AATTGGATAGCCACCGTTTGTTACTTCACGCATCTGAATTGAATTTTACTCATCCAATAACGGGGGAGGATATCGTGATT
AAAGCGGACTTTGATGAGACTTGGAAAAAACTATTTACTGAGTTTGGTTGGGATTTTTGA

Upstream 100 bases:

>100_bases
GTCTCAATTGCTCCCTATATTGAAGAAGCATTAAAGGATATTGAAGGGTATAACGAGATTGTACGCTTATGTCATGCCAT
TGATGCATTAGGTAAATGAA

Downstream 100 bases:

>100_bases
TTTTTTATTATAAAAAAGGGAAGACAGTTTATCTGTCTTCCCTTTTTTTAAATCAATAAATGAAATTACTTCATCATAGC
TTCGTTGCCGTTTTCACGAA

Product: tRNA pseudouridine synthase C

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MNTEEMTAVNDELLEEQAEPIELEIVYRDEHLIAINKPAGMLVHRSWLDKHETVFAMQTLRDQIGQHVFPLHRLDRPTSG
ILFFALSSEIAAQVSPLFASREIKKTYHAIVRGWIEEANTLDYPLKKELDKIADKKASQEVEAKEAITEYKPLAKVELPY
STGKFPTTRYCLMELKPHTGRKHQLRRHMAHLRHPIVGDTSHGDGKHNRLFKEKLDSHRLLLHASELNFTHPITGEDIVI
KADFDETWKKLFTEFGWDF

Sequences:

>Translated_259_residues
MNTEEMTAVNDELLEEQAEPIELEIVYRDEHLIAINKPAGMLVHRSWLDKHETVFAMQTLRDQIGQHVFPLHRLDRPTSG
ILFFALSSEIAAQVSPLFASREIKKTYHAIVRGWIEEANTLDYPLKKELDKIADKKASQEVEAKEAITEYKPLAKVELPY
STGKFPTTRYCLMELKPHTGRKHQLRRHMAHLRHPIVGDTSHGDGKHNRLFKEKLDSHRLLLHASELNFTHPITGEDIVI
KADFDETWKKLFTEFGWDF
>Mature_259_residues
MNTEEMTAVNDELLEEQAEPIELEIVYRDEHLIAINKPAGMLVHRSWLDKHETVFAMQTLRDQIGQHVFPLHRLDRPTSG
ILFFALSSEIAAQVSPLFASREIKKTYHAIVRGWIEEANTLDYPLKKELDKIADKKASQEVEAKEAITEYKPLAKVELPY
STGKFPTTRYCLMELKPHTGRKHQLRRHMAHLRHPIVGDTSHGDGKHNRLFKEKLDSHRLLLHASELNFTHPITGEDIVI
KADFDETWKKLFTEFGWDF

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs [H]

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family [H]

Homologues:

Organism=Homo sapiens, GI221316667, Length=220, Percent_Identity=31.8181818181818, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI17158025, Length=215, Percent_Identity=28.8372093023256, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI221316663, Length=238, Percent_Identity=29.4117647058824, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI23308689, Length=183, Percent_Identity=28.4153005464481, Blast_Score=71, Evalue=8e-13,
Organism=Escherichia coli, GI1789155, Length=235, Percent_Identity=59.1489361702128, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI1787327, Length=233, Percent_Identity=32.618025751073, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1786244, Length=224, Percent_Identity=33.9285714285714, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1788946, Length=278, Percent_Identity=28.0575539568345, Blast_Score=96, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320168, Length=180, Percent_Identity=30.5555555555556, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324506, Length=182, Percent_Identity=30.2197802197802, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6321608, Length=189, Percent_Identity=30.6878306878307, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583449, Length=185, Percent_Identity=31.8918918918919, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583447, Length=185, Percent_Identity=31.8918918918919, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583445, Length=185, Percent_Identity=31.8918918918919, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19921090, Length=183, Percent_Identity=28.9617486338798, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 30026; Mature: 30026

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS01129 PSI_RLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTEEMTAVNDELLEEQAEPIELEIVYRDEHLIAINKPAGMLVHRSWLDKHETVFAMQTL
CCCHHHHHHHHHHHHHCCCCEEEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
RDQIGQHVFPLHRLDRPTSGILFFALSSEIAAQVSPLFASREIKKTYHAIVRGWIEEANT
HHHHHHHHCCHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDYPLKKELDKIADKKASQEVEAKEAITEYKPLAKVELPYSTGKFPTTRYCLMELKPHTG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHEEEEEECCCCC
RKHQLRRHMAHLRHPIVGDTSHGDGKHNRLFKEKLDSHRLLLHASELNFTHPITGEDIVI
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEE
KADFDETWKKLFTEFGWDF
ECCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNTEEMTAVNDELLEEQAEPIELEIVYRDEHLIAINKPAGMLVHRSWLDKHETVFAMQTL
CCCHHHHHHHHHHHHHCCCCEEEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
RDQIGQHVFPLHRLDRPTSGILFFALSSEIAAQVSPLFASREIKKTYHAIVRGWIEEANT
HHHHHHHHCCHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDYPLKKELDKIADKKASQEVEAKEAITEYKPLAKVELPYSTGKFPTTRYCLMELKPHTG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHEEEEEECCCCC
RKHQLRRHMAHLRHPIVGDTSHGDGKHNRLFKEKLDSHRLLLHASELNFTHPITGEDIVI
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEE
KADFDETWKKLFTEFGWDF
ECCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]