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The map label for this gene is truB [H]

Identifier: 59711095

GI number: 59711095

Start: 524425

End: 525375

Strand: Direct

Name: truB [H]

Synonym: VF_0488

Alternate gene names: 59711095

Gene position: 524425-525375 (Clockwise)

Preceding gene: 59711094

Following gene: 59711096

Centisome position: 18.1

GC content: 42.38

Gene sequence:

>951_bases
ATGGCTCGCCGTCGTAAGGGTCGTCCGGTTAATGGTGTTATCTTAATTGATAAACCAACAGGTATTACTTCAAACGATAC
TCTGCAAAAAGTTAAACGCATTTACTTTGCTGAAAAAGCAGGTCATACAGGTGCACTTGATCCTCTAGCAACAGGTATGC
TTCCTATTTGCTTAGGTGAAGCAACGAAGTTTTCCCAGTTTCTTTTAGACTCAGATAAACGTTACCGTGTTGTCGCTAAA
CTGGGTGAACGTACGAATACTTCAGACTCAGATGGTGAAGTGGTTCAAACTCGTGAAGTAAAAGTGGATCGCGGTCAACT
TGAGCGTTGTATTGCGAAATTCCGTGGTACAACGGATCAAATTCCATCGATGTTCTCAGCATTAAAATACCAAGGTCGTC
CTTTGTATGAATATGCTCGTGAAGGCATTGAAGTACCTCGTGAGTCTCGTAAGATCACTGTTTACTCTATTGAATTACTT
CGCTTTGAAGGTCATGAAGTAGAAATGGAAGTACATTGTTCAAAAGCACTTACATTCGTACGATTACCGACGATTTTAGG
CGAAATGCTGGGTTGTGGTGCGCACGTAACTTATTTACGTCGTACTGGTGTATCGAATTATCCATATGAGAATATGGTAA
CGATTGAAGACTTAGAAGCATTACTTGAACAAGCTCATCGTGAAGAGCGTGCTCCTCGTGAGTTACTTGATCCATTACTA
ATGCCAATGGATTCAGCGGTACAAGACCTACCTGAAGTGAATATGATCCCTGAATTGGCTGATCATGTGCTTCATGGCCA
ACCGGTTCAAGTGTTTGGTGCTCCGCAAGATGGCATCGTACGTATGACATCGGGTGACGAACGTCTATTCATTGGTGTGG
GTCACATTGATGATGATGGTCGCGTTGCTCCTAAGCGCTTAGTTGTTTTTCGAGATGAAGAAGAAAAATAA

Upstream 100 bases:

>100_bases
AAGGTATGCGTATGTCTAACTTAGTAACTGATGTTGTTAATACAGATAAGCGTAAAATGGCAGAATCAGGCCGTACCGAA
TCAGATGAAGGTGAAGAGTA

Downstream 100 bases:

>100_bases
TTAATACTTAGTCTTAAAAGAATTAGAAAGAGCAAAAGCTGTTGGTTTTTGCTCTTTTTTTATTGCTTTATTATTGTTCA
TCCGTATAATTCGCTCTTCG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MARRRKGRPVNGVILIDKPTGITSNDTLQKVKRIYFAEKAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYRVVAK
LGERTNTSDSDGEVVQTREVKVDRGQLERCIAKFRGTTDQIPSMFSALKYQGRPLYEYAREGIEVPRESRKITVYSIELL
RFEGHEVEMEVHCSKALTFVRLPTILGEMLGCGAHVTYLRRTGVSNYPYENMVTIEDLEALLEQAHREERAPRELLDPLL
MPMDSAVQDLPEVNMIPELADHVLHGQPVQVFGAPQDGIVRMTSGDERLFIGVGHIDDDGRVAPKRLVVFRDEEEK

Sequences:

>Translated_316_residues
MARRRKGRPVNGVILIDKPTGITSNDTLQKVKRIYFAEKAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYRVVAK
LGERTNTSDSDGEVVQTREVKVDRGQLERCIAKFRGTTDQIPSMFSALKYQGRPLYEYAREGIEVPRESRKITVYSIELL
RFEGHEVEMEVHCSKALTFVRLPTILGEMLGCGAHVTYLRRTGVSNYPYENMVTIEDLEALLEQAHREERAPRELLDPLL
MPMDSAVQDLPEVNMIPELADHVLHGQPVQVFGAPQDGIVRMTSGDERLFIGVGHIDDDGRVAPKRLVVFRDEEEK
>Mature_315_residues
ARRRKGRPVNGVILIDKPTGITSNDTLQKVKRIYFAEKAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYRVVAKL
GERTNTSDSDGEVVQTREVKVDRGQLERCIAKFRGTTDQIPSMFSALKYQGRPLYEYAREGIEVPRESRKITVYSIELLR
FEGHEVEMEVHCSKALTFVRLPTILGEMLGCGAHVTYLRRTGVSNYPYENMVTIEDLEALLEQAHREERAPRELLDPLLM
PMDSAVQDLPEVNMIPELADHVLHGQPVQVFGAPQDGIVRMTSGDERLFIGVGHIDDDGRVAPKRLVVFRDEEEK

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=250, Percent_Identity=31.2, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI2367200, Length=310, Percent_Identity=61.9354838709677, Blast_Score=391, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17553978, Length=245, Percent_Identity=27.7551020408163, Blast_Score=81, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6324037, Length=192, Percent_Identity=32.2916666666667, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=336, Percent_Identity=26.1904761904762, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281364189, Length=235, Percent_Identity=28.936170212766, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281364187, Length=235, Percent_Identity=28.936170212766, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281364185, Length=235, Percent_Identity=28.936170212766, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281364183, Length=235, Percent_Identity=28.936170212766, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI62471759, Length=235, Percent_Identity=28.936170212766, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI17975520, Length=235, Percent_Identity=28.936170212766, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 35591; Mature: 35460

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRRKGRPVNGVILIDKPTGITSNDTLQKVKRIYFAEKAGHTGALDPLATGMLPICLGE
CCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHCC
ATKFSQFLLDSDKRYRVVAKLGERTNTSDSDGEVVQTREVKVDRGQLERCIAKFRGTTDQ
HHHHHHHHHCCCCCEEHHHHHCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHCCCHHH
IPSMFSALKYQGRPLYEYAREGIEVPRESRKITVYSIELLRFEGHEVEMEVHCSKALTFV
HHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCEEEEEEEHHHHHHHH
RLPTILGEMLGCGAHVTYLRRTGVSNYPYENMVTIEDLEALLEQAHREERAPRELLDPLL
HHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHH
MPMDSAVQDLPEVNMIPELADHVLHGQPVQVFGAPQDGIVRMTSGDERLFIGVGHIDDDG
CCHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEEECCCCEEEEEECEECCCC
RVAPKRLVVFRDEEEK
CCCCEEEEEEECCCCC
>Mature Secondary Structure 
ARRRKGRPVNGVILIDKPTGITSNDTLQKVKRIYFAEKAGHTGALDPLATGMLPICLGE
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHCC
ATKFSQFLLDSDKRYRVVAKLGERTNTSDSDGEVVQTREVKVDRGQLERCIAKFRGTTDQ
HHHHHHHHHCCCCCEEHHHHHCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHCCCHHH
IPSMFSALKYQGRPLYEYAREGIEVPRESRKITVYSIELLRFEGHEVEMEVHCSKALTFV
HHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCEEEEEEEHHHHHHHH
RLPTILGEMLGCGAHVTYLRRTGVSNYPYENMVTIEDLEALLEQAHREERAPRELLDPLL
HHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHH
MPMDSAVQDLPEVNMIPELADHVLHGQPVQVFGAPQDGIVRMTSGDERLFIGVGHIDDDG
CCHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEEECCCCEEEEEECEECCCC
RVAPKRLVVFRDEEEK
CCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA