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The map label for this gene is metK [H]

Identifier: 59711046

GI number: 59711046

Start: 465544

End: 466698

Strand: Reverse

Name: metK [H]

Synonym: VF_0439

Alternate gene names: 59711046

Gene position: 466698-465544 (Counterclockwise)

Preceding gene: 59711051

Following gene: 59711045

Centisome position: 16.11

GC content: 43.55

Gene sequence:

>1155_bases
ATGGCTAAGCACCTATTTACCTCTGAGTCAGTATCAGAAGGTCATCCAGATAAAATTGCAGATCAGATTTCAGATGCAGT
ACTTGATGCGATCTTAGAACAAGATCCAAAAGCGCGTGTTGCGTGTGAAACATACGTAAAAACTGGCATGGTGATGGTTG
GTGGTGAAGTAACCACTTCTGCATGGGTTGATATCGAAGAAATCACTCGTGAAACCGTTCGTGAGATCGGTTACGTTCAT
TCAGACATGGGCTTTGATGCAAACTCTTGTGCCGTATTAAACACAATTGGTAAGCAATCTCCAGACATCAACCAAGGTGT
TGATAAAGCTGACCCTAAAGAGCAAGGTGCTGGTGACCAAGGTATCATGTTTGGTTACGCTACTAACGAAACGCCAATTT
TAATGCCAGCTCCAATTACCTACTCTCATCTACTTGTTCAAAAACAAGCTGAAGTACGTAAAAGTGGCAAGCTAGACTTC
CTACGTCCAGATGCAAAATCTCAAGTTACATTCCAATACGACCAAGGTAAAATTGTTGGTATTGATGCAGTTGTTCTTTC
AACACAACACTGTGATTCTGTGACAACACCAGATCTTCGTGAAGCAGTAATGGAAGAAATCATCAAGCCAGTACTACCTG
CTGAATGGTTAAATAAAGACACTAACTTCTTTATTAACCCAACAGGTCGTTTTGTTATCGGTGGCCCAATGGGTGACTGT
GGTCTTACTGGTCGTAAAATTATCGTTGATACATACGGCGGTGCAGCTCGTCACGGTGGCGGTGCATTCTCTGGTAAAGA
TCCATCAAAAGTTGACCGTTCAGCAGCATACGCAGCACGTTACGTAGCGAAAAACATCGTAGCAGCTGGCATGGCTGACC
GTTGTGAAATCCAACTTTCATACGCAATCGGTGTTGCTGATCCAACATCTATCATGGTTGAAACATTCGGTACTGAGAAG
GTATCTCACGACATCATCATTGAAGCCGTTCGTCAAAACTTCGACCTACGCCCATACGGCCTACAAGAAATGCTTAACCT
TCTTCAGCCTATCTACAAGAAGACAGCAGCTTACGGTCACTTCGGTCGTGAAGAGTTCCCTTGGGAAGCGACAGATAAAG
CCGCAATCCTTCGTGATTTTGCTGGTATTAAATAA

Upstream 100 bases:

>100_bases
AGACGTCTAGATGGAAAAACACCTACACTGTTAAACGTATAGACTTATATAGATTGTGGGTATTTTTAGAACTTATTATT
TATAGAAGTGGAGCTCCCTT

Downstream 100 bases:

>100_bases
GTTTTATTTGCTTATTTCTGAATTTAAAAAGGAAGCTTCGGCTTCCTTTTTTATGTTTACAGCATGAATAATATCTTCCT
ATCTATTGATTAATCCTCTT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRETVREIGYVH
SDMGFDANSCAVLNTIGKQSPDINQGVDKADPKEQGAGDQGIMFGYATNETPILMPAPITYSHLLVQKQAEVRKSGKLDF
LRPDAKSQVTFQYDQGKIVGIDAVVLSTQHCDSVTTPDLREAVMEEIIKPVLPAEWLNKDTNFFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLSYAIGVADPTSIMVETFGTEK
VSHDIIIEAVRQNFDLRPYGLQEMLNLLQPIYKKTAAYGHFGREEFPWEATDKAAILRDFAGIK

Sequences:

>Translated_384_residues
MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRETVREIGYVH
SDMGFDANSCAVLNTIGKQSPDINQGVDKADPKEQGAGDQGIMFGYATNETPILMPAPITYSHLLVQKQAEVRKSGKLDF
LRPDAKSQVTFQYDQGKIVGIDAVVLSTQHCDSVTTPDLREAVMEEIIKPVLPAEWLNKDTNFFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLSYAIGVADPTSIMVETFGTEK
VSHDIIIEAVRQNFDLRPYGLQEMLNLLQPIYKKTAAYGHFGREEFPWEATDKAAILRDFAGIK
>Mature_383_residues
AKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTSAWVDIEEITRETVREIGYVHS
DMGFDANSCAVLNTIGKQSPDINQGVDKADPKEQGAGDQGIMFGYATNETPILMPAPITYSHLLVQKQAEVRKSGKLDFL
RPDAKSQVTFQYDQGKIVGIDAVVLSTQHCDSVTTPDLREAVMEEIIKPVLPAEWLNKDTNFFINPTGRFVIGGPMGDCG
LTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLSYAIGVADPTSIMVETFGTEKV
SHDIIIEAVRQNFDLRPYGLQEMLNLLQPIYKKTAAYGHFGREEFPWEATDKAAILRDFAGIK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=374, Percent_Identity=56.4171122994652, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI4557737, Length=374, Percent_Identity=55.8823529411765, Blast_Score=404, Evalue=1e-113,
Organism=Escherichia coli, GI1789311, Length=384, Percent_Identity=85.15625, Blast_Score=684, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17538498, Length=384, Percent_Identity=55.46875, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25145633, Length=384, Percent_Identity=55.2083333333333, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17538494, Length=377, Percent_Identity=55.9681697612732, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17551082, Length=373, Percent_Identity=54.6916890080429, Blast_Score=393, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI32565905, Length=373, Percent_Identity=50.9383378016086, Blast_Score=339, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI32565911, Length=337, Percent_Identity=51.9287833827893, Blast_Score=327, Evalue=8e-90,
Organism=Caenorhabditis elegans, GI25145635, Length=337, Percent_Identity=51.6320474777448, Blast_Score=325, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI32565903, Length=299, Percent_Identity=53.1772575250836, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=60.9195402298851, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=46, Percent_Identity=73.9130434782609, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323209, Length=370, Percent_Identity=58.1081081081081, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6320710, Length=374, Percent_Identity=57.4866310160428, Blast_Score=413, Evalue=1e-116,
Organism=Drosophila melanogaster, GI45552159, Length=373, Percent_Identity=55.4959785522788, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580529, Length=373, Percent_Identity=55.4959785522788, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580537, Length=373, Percent_Identity=55.4959785522788, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580533, Length=373, Percent_Identity=55.4959785522788, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580535, Length=373, Percent_Identity=55.4959785522788, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580531, Length=373, Percent_Identity=55.4959785522788, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580527, Length=373, Percent_Identity=54.9597855227882, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580525, Length=373, Percent_Identity=54.9597855227882, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580541, Length=372, Percent_Identity=52.6881720430108, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580539, Length=120, Percent_Identity=55.8333333333333, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41828; Mature: 41697

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCCH
AWVDIEEITRETVREIGYVHSDMGFDANSCAVLNTIGKQSPDINQGVDKADPKEQGAGDQ
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHCCCCCCCCHHHCCCCC
GIMFGYATNETPILMPAPITYSHLLVQKQAEVRKSGKLDFLRPDAKSQVTFQYDQGKIVG
CEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEECCCEEEE
IDAVVLSTQHCDSVTTPDLREAVMEEIIKPVLPAEWLNKDTNFFINPTGRFVIGGPMGDC
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLS
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVADPTSIMVETFGTEKVSHDIIIEAVRQNFDLRPYGLQEMLNLLQPIYKKTAAYGH
EEEECCCCHHHEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
FGREEFPWEATDKAAILRDFAGIK
CCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
AKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACETYVKTGMVMVGGEVTTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEECCCCCCH
AWVDIEEITRETVREIGYVHSDMGFDANSCAVLNTIGKQSPDINQGVDKADPKEQGAGDQ
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHCCCCCCCCHHHCCCCC
GIMFGYATNETPILMPAPITYSHLLVQKQAEVRKSGKLDFLRPDAKSQVTFQYDQGKIVG
CEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCEEEEECCCEEEE
IDAVVLSTQHCDSVTTPDLREAVMEEIIKPVLPAEWLNKDTNFFINPTGRFVIGGPMGDC
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGMADRCEIQLS
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVADPTSIMVETFGTEKVSHDIIIEAVRQNFDLRPYGLQEMLNLLQPIYKKTAAYGH
EEEECCCCHHHEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
FGREEFPWEATDKAAILRDFAGIK
CCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA