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The map label for this gene is rsmE [H]

Identifier: 59711043

GI number: 59711043

Start: 463277

End: 464008

Strand: Reverse

Name: rsmE [H]

Synonym: VF_0436

Alternate gene names: 59711043

Gene position: 464008-463277 (Counterclockwise)

Preceding gene: 59711044

Following gene: 59711042

Centisome position: 16.01

GC content: 41.53

Gene sequence:

>732_bases
ATGAGAATACCAAGAATCCACCACCCAGAGCTTCTTCCTTCACAAGGAAAAGTAAATTTAAGTGATGATGCAGCAAATCA
CGTTGGTCGTGTAATGCGAATGAAAGAAGGTGAATCTGTGCTTCTGTTTGATGGAAGCGGATCTGAATTTCCAGCCGTGA
TCACTTCAGCGTCAAAAAAGAGTGTTGAAGTGGAAATCACTGAGCGTATTGAGCGTAGCAGCGAATCGCCACTTAACTTA
CATTTAGGTCAGGTTGTTTCTCGTGGCGATAAAATGGAATTTACCATTCAAAAATCGGTCGAATTGGGTGTGAATATCAT
TACACCACTGATCTCTGAGCGCTGTGGCGTTAAATTAAATAAAGAGCGATTTGAAAAGAAATTAGATCAATGGCAAAAAA
TTGCAATTGCTGCATGTGAGCAATGTGGACGTAATACTGTTCCTGAAATTCGTCCTATTATGTCATTAGAAGAATGGTGC
GCAGAAGAGTACGATGGGTTAAAGCTCAACCTTCATCCACGAGCAAAATACTCAATCAATACACTTCCTGAACCGGTAAC
AAATGTACGCTTGTTAATTGGTCCTGAAGGCGGTTTATCGTCTGATGAAATTAGCATGACAGAACAATATCAATTTGAAG
AAACGCTGCTTGGCCCTCGAGTTCTACGAACAGAAACCGCAGCGCTTACCGCAATTACAGCCCTACAAGTTCGTTTTGGC
GATCTTGGCTAA

Upstream 100 bases:

>100_bases
TTTGTCTACAAAGCATGTCGCAAATAGACATAATTAGAGGTTGAAAAAGCATACTTTAACCTCCATCCTTAAATACAAGA
TAGTTAAAGAAAAGTAGACC

Downstream 100 bases:

>100_bases
CTGGAGAAACACAATGATCAAACTCGGTATCGTAATGGATCCAATTTCATCCATTAACATTAAAAAAGACTCTAGCTTTG
CCATGATGCTTGAAGCTCAA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRIPRIHHPELLPSQGKVNLSDDAANHVGRVMRMKEGESVLLFDGSGSEFPAVITSASKKSVEVEITERIERSSESPLNL
HLGQVVSRGDKMEFTIQKSVELGVNIITPLISERCGVKLNKERFEKKLDQWQKIAIAACEQCGRNTVPEIRPIMSLEEWC
AEEYDGLKLNLHPRAKYSINTLPEPVTNVRLLIGPEGGLSSDEISMTEQYQFEETLLGPRVLRTETAALTAITALQVRFG
DLG

Sequences:

>Translated_243_residues
MRIPRIHHPELLPSQGKVNLSDDAANHVGRVMRMKEGESVLLFDGSGSEFPAVITSASKKSVEVEITERIERSSESPLNL
HLGQVVSRGDKMEFTIQKSVELGVNIITPLISERCGVKLNKERFEKKLDQWQKIAIAACEQCGRNTVPEIRPIMSLEEWC
AEEYDGLKLNLHPRAKYSINTLPEPVTNVRLLIGPEGGLSSDEISMTEQYQFEETLLGPRVLRTETAALTAITALQVRFG
DLG
>Mature_243_residues
MRIPRIHHPELLPSQGKVNLSDDAANHVGRVMRMKEGESVLLFDGSGSEFPAVITSASKKSVEVEITERIERSSESPLNL
HLGQVVSRGDKMEFTIQKSVELGVNIITPLISERCGVKLNKERFEKKLDQWQKIAIAACEQCGRNTVPEIRPIMSLEEWC
AEEYDGLKLNLHPRAKYSINTLPEPVTNVRLLIGPEGGLSSDEISMTEQYQFEETLLGPRVLRTETAALTAITALQVRFG
DLG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=243, Percent_Identity=73.2510288065844, Blast_Score=365, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27077; Mature: 27077

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPRIHHPELLPSQGKVNLSDDAANHVGRVMRMKEGESVLLFDGSGSEFPAVITSASKK
CCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEECCCCC
SVEVEITERIERSSESPLNLHLGQVVSRGDKMEFTIQKSVELGVNIITPLISERCGVKLN
EEEEEHHHHHHCCCCCCCEEHHHHHHCCCCCEEEEEECHHHCCHHHHHHHHHHHCCCEEC
KERFEKKLDQWQKIAIAACEQCGRNTVPEIRPIMSLEEWCAEEYDGLKLNLHPRAKYSIN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCEECC
TLPEPVTNVRLLIGPEGGLSSDEISMTEQYQFEETLLGPRVLRTETAALTAITALQVRFG
CCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECC
DLG
CCC
>Mature Secondary Structure
MRIPRIHHPELLPSQGKVNLSDDAANHVGRVMRMKEGESVLLFDGSGSEFPAVITSASKK
CCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEECCCCC
SVEVEITERIERSSESPLNLHLGQVVSRGDKMEFTIQKSVELGVNIITPLISERCGVKLN
EEEEEHHHHHHCCCCCCCEEHHHHHHCCCCCEEEEEECHHHCCHHHHHHHHHHHCCCEEC
KERFEKKLDQWQKIAIAACEQCGRNTVPEIRPIMSLEEWCAEEYDGLKLNLHPRAKYSIN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCEECC
TLPEPVTNVRLLIGPEGGLSSDEISMTEQYQFEETLLGPRVLRTETAALTAITALQVRFG
CCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECC
DLG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]