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The map label for this gene is gshB [H]

Identifier: 59711042

GI number: 59711042

Start: 462316

End: 463263

Strand: Reverse

Name: gshB [H]

Synonym: VF_0435

Alternate gene names: 59711042

Gene position: 463263-462316 (Counterclockwise)

Preceding gene: 59711043

Following gene: 59711041

Centisome position: 15.99

GC content: 41.14

Gene sequence:

>948_bases
ATGATCAAACTCGGTATCGTAATGGATCCAATTTCATCCATTAACATTAAAAAAGACTCTAGCTTTGCCATGATGCTTGA
AGCTCAAAAACGTGGTTGGGAAATTCATTACATGGAAATGAGTGACCTACATTTAGCTCAAGGCAAAGCGGTCGCTGATA
CCAAAATCGTTACGCTAAAAGATGATCCAACAGGTTGGTATGAATTCCAATCAGAGCAAACCATCGACCTTGCTGATCTT
GATGCGGTATTAATGCGTAAAGATCCACCTTTCGATACAGAATACATCTATGCGACATATATTCTAGAAAGAGCTGAAAT
CCAAGGTGCACTGATTGTTAATAAACCTCAGAGTCTACGTGATTGTAACGAGAAACTATTCACGGCTTGGTTTCCAGAGC
TAACGCCAACAACATTAGTGACTCGTAAGGCTGAAAAAATTAAAGCTTTCCGTGAAGAGCATGGAGATGTAATTCTTAAA
CCACTAGATGGTATGGGGGGCGCTTCTATTTTCCGAGTAAAAGCAGGCGATCCAAATGTATCAGTGATCATCGAAACACT
AACCAATCACGGACAAAACTACTGCATGGCACAAACCTTTGTCCCTGATATTAGTAACGGTGATAAGCGTATTCTTGTGG
TTGATGGTGAGCCAATGCCTTACTGTTTAGCTCGAATTCCAGCAAAAGGTGAAACTCGCGGCAACTTAGCAGCAGGTGGT
ACAGGTGAAGCACGTCCATTAAGTGAGACAGATTGGGCAATTGCTAATGCAGTAGCTCCAACATTGAAAGAAAAGGGTCT
AATCTTTGTAGGACTCGACGTGATTGGCGACAAGCTAACTGAGATTAATGTAACAAGCCCAACCTGTATTAAGGAAATTG
AAGCTGCTTTTGATATTTCGATTACAGGCAAATTAATGGATGCAATTGAGCGCCGAATTAACGCTTAA

Upstream 100 bases:

>100_bases
CTGCTTGGCCCTCGAGTTCTACGAACAGAAACCGCAGCGCTTACCGCAATTACAGCCCTACAAGTTCGTTTTGGCGATCT
TGGCTAACTGGAGAAACACA

Downstream 100 bases:

>100_bases
TCACATTCTAACTGTATTGAGGGGGCAACTCCTCAATATTAATACCAATTGGTTTATGCGTTATCTTTAGCGGTATTTAC
ATTGGTATTAATCTCGCTTT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MIKLGIVMDPISSINIKKDSSFAMMLEAQKRGWEIHYMEMSDLHLAQGKAVADTKIVTLKDDPTGWYEFQSEQTIDLADL
DAVLMRKDPPFDTEYIYATYILERAEIQGALIVNKPQSLRDCNEKLFTAWFPELTPTTLVTRKAEKIKAFREEHGDVILK
PLDGMGGASIFRVKAGDPNVSVIIETLTNHGQNYCMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
TGEARPLSETDWAIANAVAPTLKEKGLIFVGLDVIGDKLTEINVTSPTCIKEIEAAFDISITGKLMDAIERRINA

Sequences:

>Translated_315_residues
MIKLGIVMDPISSINIKKDSSFAMMLEAQKRGWEIHYMEMSDLHLAQGKAVADTKIVTLKDDPTGWYEFQSEQTIDLADL
DAVLMRKDPPFDTEYIYATYILERAEIQGALIVNKPQSLRDCNEKLFTAWFPELTPTTLVTRKAEKIKAFREEHGDVILK
PLDGMGGASIFRVKAGDPNVSVIIETLTNHGQNYCMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
TGEARPLSETDWAIANAVAPTLKEKGLIFVGLDVIGDKLTEINVTSPTCIKEIEAAFDISITGKLMDAIERRINA
>Mature_315_residues
MIKLGIVMDPISSINIKKDSSFAMMLEAQKRGWEIHYMEMSDLHLAQGKAVADTKIVTLKDDPTGWYEFQSEQTIDLADL
DAVLMRKDPPFDTEYIYATYILERAEIQGALIVNKPQSLRDCNEKLFTAWFPELTPTTLVTRKAEKIKAFREEHGDVILK
PLDGMGGASIFRVKAGDPNVSVIIETLTNHGQNYCMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
TGEARPLSETDWAIANAVAPTLKEKGLIFVGLDVIGDKLTEINVTSPTCIKEIEAAFDISITGKLMDAIERRINA

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=314, Percent_Identity=73.2484076433121, Blast_Score=491, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34777; Mature: 34777

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLGIVMDPISSINIKKDSSFAMMLEAQKRGWEIHYMEMSDLHLAQGKAVADTKIVTLK
CEEEEEEECCCCCCEEECCCCEEEEEEHHHCCCEEEEEEECCEEEECCCEECCEEEEEEE
DDPTGWYEFQSEQTIDLADLDAVLMRKDPPFDTEYIYATYILERAEIQGALIVNKPQSLR
CCCCCCEEECCCCEEEHHHCCHHEECCCCCCCCCEEEEEEEEEHHHCCEEEEEECCHHHH
DCNEKLFTAWFPELTPTTLVTRKAEKIKAFREEHGDVILKPLDGMGGASIFRVKAGDPNV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCC
SVIIETLTNHGQNYCMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
EEEEEEHHCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCHHEEECCCCCCCCCCEEECC
TGEARPLSETDWAIANAVAPTLKEKGLIFVGLDVIGDKLTEINVTSPTCIKEIEAAFDIS
CCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEHHHHCCCEEEEECCCCHHHHHHHHHEEEE
ITGKLMDAIERRINA
EHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKLGIVMDPISSINIKKDSSFAMMLEAQKRGWEIHYMEMSDLHLAQGKAVADTKIVTLK
CEEEEEEECCCCCCEEECCCCEEEEEEHHHCCCEEEEEEECCEEEECCCEECCEEEEEEE
DDPTGWYEFQSEQTIDLADLDAVLMRKDPPFDTEYIYATYILERAEIQGALIVNKPQSLR
CCCCCCEEECCCCEEEHHHCCHHEECCCCCCCCCEEEEEEEEEHHHCCEEEEEECCHHHH
DCNEKLFTAWFPELTPTTLVTRKAEKIKAFREEHGDVILKPLDGMGGASIFRVKAGDPNV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCC
SVIIETLTNHGQNYCMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
EEEEEEHHCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCHHEEECCCCCCCCCCEEECC
TGEARPLSETDWAIANAVAPTLKEKGLIFVGLDVIGDKLTEINVTSPTCIKEIEAAFDIS
CCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEHHHHCCCEEEEECCCCHHHHHHHHHEEEE
ITGKLMDAIERRINA
EHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]