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The map label for this gene is rpoN [H]

Identifier: 59710994

GI number: 59710994

Start: 420910

End: 422379

Strand: Reverse

Name: rpoN [H]

Synonym: VF_0387

Alternate gene names: 59710994

Gene position: 422379-420910 (Counterclockwise)

Preceding gene: 59710995

Following gene: 59710993

Centisome position: 14.58

GC content: 40.07

Gene sequence:

>1470_bases
ATGAAAGCATCTCTTCAGCTAAAAATGGGACAACAACTTGCTATGACGCCTCAGTTGCAGCAAGCTATTCGATTATTGCA
ATTATCGACCCTTGACCTACAGCAAGAGATTCAAGAAGCGCTCGACTCAAATCCACTTCTAGATGTAGAAGAGGAAGCAT
TAAGCACACCTGAAACTCTTACCTCTCCTGAGCCTAAATCAGAAAAAGAAACCGCATCAGCAGAGCAAGAAACACCAATC
ACAGATAGTAGTGATGTCATCGAAAGTAATAACATTTCAGAAGAACTTGAAATGGATGCTAGTTGGGATGATGTTTATAG
CGCAAACAGTGGAAGCACTGGATTAGCCATTGATGACGATACCCCTATTTATCAAGGTGAAACCACAGAAAGCTTGCAAG
ATTATCTGATGTGGCAAGCTGACTTAACCCCATTTACCGATCTTGATCGAACAATCGCAACCACGATTATCGAGTCATTA
GATGAATACGGTTACCTGACTAGTTCTCTTGACGATATTTTAGAAAGTATTGGAGATGAAGAGGTTGAAATGGATGAAGT
TGAAGCGGTTTTAAAACGCATTCAACAATTTGATCCATTAGGTGTTGCTTCTCGTGATTTGGCAGAGTGTCTATTACTTC
AACTTGCAACTTACCCTGCTAATACCCCTTGGTTACCTGAAACTAAGCTGATTTTAAAAGATCATATTAATTTATTAGGA
AATCGAGACTACCGACAACTAGCAAAAGAGACTAAGCTTAAAGAAAGTGACTTAAAGCAAGTCATGATGCTTATTCACGA
GTTAGATCCTCGCCCAGGTAATCGTGTTATCGATACTGAAACTGAGTACGTTATTCCTGATGTTTCTGTATTCAAACACA
ATGGAAAATGGGTTGTAACCATTAACCCTGACAGTGTTCCTCGCTTAAAAGTAAATGCGGAATATGCGGCTCTTGGTAAG
ACAATGGGCAACACTCCGGATGGGCAATTCATTCGAACCAATTTGCAAGAGGCAAAGTGGTTAATTAAGAGCCTAGAGAG
CAGAAACGAGACGCTGCTCAAAGTTGCACGTTGTATAGTTGAACATCAACAAGATTTCTTCGAATATGGTGAAGAAGCAA
TGAAACCTATGGTTTTAAATGACATCGCATTAGATGTCGATATGCACGAATCCACCATATCACGAGTAACAACACAGAAG
TTCATGCATACCCCTCGAGGTATTTTTGAATTGAAATATTTCTTCTCTAGTCATGTTAGTACGGATAATGGTGGTGAATG
CTCATCTACAGCAATCCGAGCACTAGTGAAAAAATTGGTTGCGGCTGAGAATCAAGCTAAGCCGTTAAGTGATAGTAAAA
TTGCAACCTTACTGGCTGAACAAGGAATCCAAGTAGCACGCCGTACCATTGCTAAATATCGTGAATCACTTGGTATCGCA
CCATCAAATCAGCGTAAGCGTTTACTTTAA

Upstream 100 bases:

>100_bases
CAAATGGCACACCTGAACACGTATTAAATGATGAACACGTAAAACGTGTTTATCTTGGTGATCAATTTAAGTTGTAATGT
ACAAAAGGTATCGCTAAATA

Downstream 100 bases:

>100_bases
TTAATGAAAAGGAAGTGTTATGCAAATAAACATCGCAGGTCAAAACGTCGAACTAACCGATAGCCTTCGTGAGTATGTGA
ATACCAAATTCCAAAAATTA

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MKASLQLKMGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALDSNPLLDVEEEALSTPETLTSPEPKSEKETASAEQETPI
TDSSDVIESNNISEELEMDASWDDVYSANSGSTGLAIDDDTPIYQGETTESLQDYLMWQADLTPFTDLDRTIATTIIESL
DEYGYLTSSLDDILESIGDEEVEMDEVEAVLKRIQQFDPLGVASRDLAECLLLQLATYPANTPWLPETKLILKDHINLLG
NRDYRQLAKETKLKESDLKQVMMLIHELDPRPGNRVIDTETEYVIPDVSVFKHNGKWVVTINPDSVPRLKVNAEYAALGK
TMGNTPDGQFIRTNLQEAKWLIKSLESRNETLLKVARCIVEHQQDFFEYGEEAMKPMVLNDIALDVDMHESTISRVTTQK
FMHTPRGIFELKYFFSSHVSTDNGGECSSTAIRALVKKLVAAENQAKPLSDSKIATLLAEQGIQVARRTIAKYRESLGIA
PSNQRKRLL

Sequences:

>Translated_489_residues
MKASLQLKMGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALDSNPLLDVEEEALSTPETLTSPEPKSEKETASAEQETPI
TDSSDVIESNNISEELEMDASWDDVYSANSGSTGLAIDDDTPIYQGETTESLQDYLMWQADLTPFTDLDRTIATTIIESL
DEYGYLTSSLDDILESIGDEEVEMDEVEAVLKRIQQFDPLGVASRDLAECLLLQLATYPANTPWLPETKLILKDHINLLG
NRDYRQLAKETKLKESDLKQVMMLIHELDPRPGNRVIDTETEYVIPDVSVFKHNGKWVVTINPDSVPRLKVNAEYAALGK
TMGNTPDGQFIRTNLQEAKWLIKSLESRNETLLKVARCIVEHQQDFFEYGEEAMKPMVLNDIALDVDMHESTISRVTTQK
FMHTPRGIFELKYFFSSHVSTDNGGECSSTAIRALVKKLVAAENQAKPLSDSKIATLLAEQGIQVARRTIAKYRESLGIA
PSNQRKRLL
>Mature_489_residues
MKASLQLKMGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALDSNPLLDVEEEALSTPETLTSPEPKSEKETASAEQETPI
TDSSDVIESNNISEELEMDASWDDVYSANSGSTGLAIDDDTPIYQGETTESLQDYLMWQADLTPFTDLDRTIATTIIESL
DEYGYLTSSLDDILESIGDEEVEMDEVEAVLKRIQQFDPLGVASRDLAECLLLQLATYPANTPWLPETKLILKDHINLLG
NRDYRQLAKETKLKESDLKQVMMLIHELDPRPGNRVIDTETEYVIPDVSVFKHNGKWVVTINPDSVPRLKVNAEYAALGK
TMGNTPDGQFIRTNLQEAKWLIKSLESRNETLLKVARCIVEHQQDFFEYGEEAMKPMVLNDIALDVDMHESTISRVTTQK
FMHTPRGIFELKYFFSSHVSTDNGGECSSTAIRALVKKLVAAENQAKPLSDSKIATLLAEQGIQVARRTIAKYRESLGIA
PSNQRKRLL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=491, Percent_Identity=63.7474541751528, Blast_Score=644, Evalue=0.0,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 54884; Mature: 54884

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASLQLKMGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALDSNPLLDVEEEALSTPETL
CCCCEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCC
TSPEPKSEKETASAEQETPITDSSDVIESNNISEELEMDASWDDVYSANSGSTGLAIDDD
CCCCCCCHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHCCCCHHHHHCCCCCCCCEEECCC
TPIYQGETTESLQDYLMWQADLTPFTDLDRTIATTIIESLDEYGYLTSSLDDILESIGDE
CCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCC
EVEMDEVEAVLKRIQQFDPLGVASRDLAECLLLQLATYPANTPWLPETKLILKDHINLLG
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHC
NRDYRQLAKETKLKESDLKQVMMLIHELDPRPGNRVIDTETEYVIPDVSVFKHNGKWVVT
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCEECCCHHHEEECCEEEEE
INPDSVPRLKVNAEYAALGKTMGNTPDGQFIRTNLQEAKWLIKSLESRNETLLKVARCIV
ECCCCCCEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
EHQQDFFEYGEEAMKPMVLNDIALDVDMHESTISRVTTQKFMHTPRGIFELKYFFSSHVS
HHHHHHHHHHHHHCCCHHHHHHEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
TDNGGECSSTAIRALVKKLVAAENQAKPLSDSKIATLLAEQGIQVARRTIAKYRESLGIA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PSNQRKRLL
CCCCCCCCC
>Mature Secondary Structure
MKASLQLKMGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALDSNPLLDVEEEALSTPETL
CCCCEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCC
TSPEPKSEKETASAEQETPITDSSDVIESNNISEELEMDASWDDVYSANSGSTGLAIDDD
CCCCCCCHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHCCCCHHHHHCCCCCCCCEEECCC
TPIYQGETTESLQDYLMWQADLTPFTDLDRTIATTIIESLDEYGYLTSSLDDILESIGDE
CCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCC
EVEMDEVEAVLKRIQQFDPLGVASRDLAECLLLQLATYPANTPWLPETKLILKDHINLLG
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHC
NRDYRQLAKETKLKESDLKQVMMLIHELDPRPGNRVIDTETEYVIPDVSVFKHNGKWVVT
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCEECCCHHHEEECCEEEEE
INPDSVPRLKVNAEYAALGKTMGNTPDGQFIRTNLQEAKWLIKSLESRNETLLKVARCIV
ECCCCCCEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
EHQQDFFEYGEEAMKPMVLNDIALDVDMHESTISRVTTQKFMHTPRGIFELKYFFSSHVS
HHHHHHHHHHHHHCCCHHHHHHEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
TDNGGECSSTAIRALVKKLVAAENQAKPLSDSKIATLLAEQGIQVARRTIAKYRESLGIA
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PSNQRKRLL
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9421909 [H]