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The map label for this gene is imp

Identifier: 59710896

GI number: 59710896

Start: 293582

End: 295942

Strand: Reverse

Name: imp

Synonym: VF_0289

Alternate gene names: 59710896

Gene position: 295942-293582 (Counterclockwise)

Preceding gene: 59710898

Following gene: 59710895

Centisome position: 10.21

GC content: 37.36

Gene sequence:

>2361_bases
ATGTCTTTTACTTCTCGTAGCCTACTTGCCTCATTTACTGGATGCCTTCTTTACGGCACTCCTGCCATTGCTGATAATGG
TAGTGAACCTGTATCTCCAGAAAATGGCATTGTTTTTCCTCTGCAAACTGATGCAGTTAACTCTTGTGAAGTCCCGGATA
AAAAATCAAAAGAGGAAGAAGATCAACAACCTGTATTAATTCAAGCAGACAGTGTTGAAGCGAGCAACAATTCAAAAGCC
GTTTATAAAGGTAATGTTCATATTATTAAAGGCCGACAAGAAATTAAAGCCGATAGCATTACTTTACATCAACAAGAAAA
CATCGCAATTGCTGAAGGTAATGTTGATTACTCAAGCATGGAGATGAGAACCACCTCAGATAAAGTAACCACTAACTTAT
CGACTGAAGACACCACTATGGTGAATACGGCATATAAAATGTCGTGCCAACCAATTCGTGGACAAGCTGGACGCGTATTA
AAGACAGGTCAAGAAATCTATCAATTAGAAGACGCCTCTTTTACAACCTGTCCTGCTGACGATAATAGTTGGCGCTTTAA
AGCCAGTGATATTGAATTAGACCAATCAGACGAATGGGCAACATTCTATAATGCTCGTTTTGAAGTACTTGATGTACCTA
TCTTCTACTTACCTTATGTTACGGTACCGGTTGGCGATACTCGTAAAACCGGTGTATTGATCCCTAGCATTGGTCTTGAT
AGTAAAAATGGTTTTGAACTTTCGGTCCCTATTTACTGGAATATTGCACCAAATTACGATGCAACCACGACCATTAACTA
TATGGAAAGACGCGGTACTCAATTGGAAACTGAATTCCGATACTTAACTGAGTTAGGTAAAGGTACTGTTGATGCTGAAT
ACTTAAATGAAGATGATAAATTCAAAGATAAAGGTTCTCGTTGGGGTGTTAGCTGGGATCATTCTGGTATTTATCAACAA
CACTGGAAATTTGATGTTGAGTATTCAAAAGTAAGTGATATTGATTACTTCCAAGACCTCAATTCAAGTATTGGTACTCG
TGATGAAGGCCAACTACAACAGTCAGGTGAAGTTTCCTATCGTAGCCAAGATTGGGATATGACCATGCGTGTTCGTGACT
TCCAAGTACTGGTTGAAGAACAAACACCATACCGATTAATGCCGCAAATTGAGTTTAACTATTACGCTCCTCAATTCTAT
AGCGAGTTTGATTTTAATTTACATAGTCACATTAGTAAGTTTACTACTGATGATAAAGCTAAACCTTCTGCCACCCGAGT
TCATTTAGAACCTAAATTGAGTTTACCTTTAAGTGGAACTTGGTGGTCTTTAATACCTGAAACAAGTTTACTTTATACTT
ATTATCAACAAGATTTTGATAAGCAACCAGTTGGACCTAATGGTTCTTTAAACTTAGATCATGAAGTTAGCAGAACTATT
CCTGAAGTTCGTATTAACGGGGCTATTTATTTAGATAGTACGCATAAATTCTTAGGTGAGTACTTACAAACTTTAGAACC
CAAAATACAGTACCTCTATGTACCCGAAGTGGATCAAAGTAATATTTATGGTGGTACTGGTGACGGTGGTTACGACAGCA
GTAAATTACAACTAGATTATTATGGTTTATTTAGAGATCGTCAGTACAGTGGCGTGGACTATATTGCTGATGCGAATCAA
TTTAGTGTTGGTGCAACCTCTCGTTTCTATGATGATGCGTATAAAGAAAGAATGAATATTTCTTTTGGTCAAATACTGTA
CTTGAATGGTTCAGGTACAGAGCAAAATAATGATGATAAAAACTCATCCGCATGGGCAATGGAAAGTGATTTTAACTATG
ATGATTACCTCTTCTATCATGGTGGTATTCAATACGACTCAAATGTAAGTGAACTTCAAGTCGCTAATAGCACACTTGAA
TATCGCTTTTCAAAGGGTTATATCCAAGCAAACTATCGCTATGTGAGTAAAAACTACATTGAAAGTAACGTAAACTTTGA
AGATGACCTGAGTCTCATTACACAGCACGGTATCTATCAAGCTGGTCTTTTAAGCGAATATAATCTAGGAAGAAATTGGG
CTCTTAAAGGTCAATATTTCCACGATACAAAAGAAGACCAAATGATTGAAGCGTTAGTAGGTGTTACTTACCTTAGTGAC
TGTTGGTCATTTGGATTAACTTACAGCGACCAATTAATTGCACCAGAATCTGCAAAAACCATTGGAACTTATGAACCTGA
ATATGAAAGTAACTTAATGCTTTCTATTGCTATTCGTGGCTTAGGTAACAATACGGGAATTACATCTGGCTCTGCGAATA
ACGCCTTAGACTACGGTCGCCCGTTCTACCTAAATAATTAA

Upstream 100 bases:

>100_bases
GGTTTGGGCATAGGTTTTGCTTTATTATAAAGAAGTGACTCTGTCTAGTATCTTTGAGTATATCAGCGGATTAATATAAC
AAACAGGAACGTCTAACTCG

Downstream 100 bases:

>100_bases
TTATATGGAAATACATGAAGAGCCAACACTCTAAGGCTCTTCATTCTCCTTACTCTTGGATGACACTATGAAAAACTGGA
AATTCCCACTGATTAGCACA

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein

Number of amino acids: Translated: 786; Mature: 785

Protein sequence:

>786_residues
MSFTSRSLLASFTGCLLYGTPAIADNGSEPVSPENGIVFPLQTDAVNSCEVPDKKSKEEEDQQPVLIQADSVEASNNSKA
VYKGNVHIIKGRQEIKADSITLHQQENIAIAEGNVDYSSMEMRTTSDKVTTNLSTEDTTMVNTAYKMSCQPIRGQAGRVL
KTGQEIYQLEDASFTTCPADDNSWRFKASDIELDQSDEWATFYNARFEVLDVPIFYLPYVTVPVGDTRKTGVLIPSIGLD
SKNGFELSVPIYWNIAPNYDATTTINYMERRGTQLETEFRYLTELGKGTVDAEYLNEDDKFKDKGSRWGVSWDHSGIYQQ
HWKFDVEYSKVSDIDYFQDLNSSIGTRDEGQLQQSGEVSYRSQDWDMTMRVRDFQVLVEEQTPYRLMPQIEFNYYAPQFY
SEFDFNLHSHISKFTTDDKAKPSATRVHLEPKLSLPLSGTWWSLIPETSLLYTYYQQDFDKQPVGPNGSLNLDHEVSRTI
PEVRINGAIYLDSTHKFLGEYLQTLEPKIQYLYVPEVDQSNIYGGTGDGGYDSSKLQLDYYGLFRDRQYSGVDYIADANQ
FSVGATSRFYDDAYKERMNISFGQILYLNGSGTEQNNDDKNSSAWAMESDFNYDDYLFYHGGIQYDSNVSELQVANSTLE
YRFSKGYIQANYRYVSKNYIESNVNFEDDLSLITQHGIYQAGLLSEYNLGRNWALKGQYFHDTKEDQMIEALVGVTYLSD
CWSFGLTYSDQLIAPESAKTIGTYEPEYESNLMLSIAIRGLGNNTGITSGSANNALDYGRPFYLNN

Sequences:

>Translated_786_residues
MSFTSRSLLASFTGCLLYGTPAIADNGSEPVSPENGIVFPLQTDAVNSCEVPDKKSKEEEDQQPVLIQADSVEASNNSKA
VYKGNVHIIKGRQEIKADSITLHQQENIAIAEGNVDYSSMEMRTTSDKVTTNLSTEDTTMVNTAYKMSCQPIRGQAGRVL
KTGQEIYQLEDASFTTCPADDNSWRFKASDIELDQSDEWATFYNARFEVLDVPIFYLPYVTVPVGDTRKTGVLIPSIGLD
SKNGFELSVPIYWNIAPNYDATTTINYMERRGTQLETEFRYLTELGKGTVDAEYLNEDDKFKDKGSRWGVSWDHSGIYQQ
HWKFDVEYSKVSDIDYFQDLNSSIGTRDEGQLQQSGEVSYRSQDWDMTMRVRDFQVLVEEQTPYRLMPQIEFNYYAPQFY
SEFDFNLHSHISKFTTDDKAKPSATRVHLEPKLSLPLSGTWWSLIPETSLLYTYYQQDFDKQPVGPNGSLNLDHEVSRTI
PEVRINGAIYLDSTHKFLGEYLQTLEPKIQYLYVPEVDQSNIYGGTGDGGYDSSKLQLDYYGLFRDRQYSGVDYIADANQ
FSVGATSRFYDDAYKERMNISFGQILYLNGSGTEQNNDDKNSSAWAMESDFNYDDYLFYHGGIQYDSNVSELQVANSTLE
YRFSKGYIQANYRYVSKNYIESNVNFEDDLSLITQHGIYQAGLLSEYNLGRNWALKGQYFHDTKEDQMIEALVGVTYLSD
CWSFGLTYSDQLIAPESAKTIGTYEPEYESNLMLSIAIRGLGNNTGITSGSANNALDYGRPFYLNN
>Mature_785_residues
SFTSRSLLASFTGCLLYGTPAIADNGSEPVSPENGIVFPLQTDAVNSCEVPDKKSKEEEDQQPVLIQADSVEASNNSKAV
YKGNVHIIKGRQEIKADSITLHQQENIAIAEGNVDYSSMEMRTTSDKVTTNLSTEDTTMVNTAYKMSCQPIRGQAGRVLK
TGQEIYQLEDASFTTCPADDNSWRFKASDIELDQSDEWATFYNARFEVLDVPIFYLPYVTVPVGDTRKTGVLIPSIGLDS
KNGFELSVPIYWNIAPNYDATTTINYMERRGTQLETEFRYLTELGKGTVDAEYLNEDDKFKDKGSRWGVSWDHSGIYQQH
WKFDVEYSKVSDIDYFQDLNSSIGTRDEGQLQQSGEVSYRSQDWDMTMRVRDFQVLVEEQTPYRLMPQIEFNYYAPQFYS
EFDFNLHSHISKFTTDDKAKPSATRVHLEPKLSLPLSGTWWSLIPETSLLYTYYQQDFDKQPVGPNGSLNLDHEVSRTIP
EVRINGAIYLDSTHKFLGEYLQTLEPKIQYLYVPEVDQSNIYGGTGDGGYDSSKLQLDYYGLFRDRQYSGVDYIADANQF
SVGATSRFYDDAYKERMNISFGQILYLNGSGTEQNNDDKNSSAWAMESDFNYDDYLFYHGGIQYDSNVSELQVANSTLEY
RFSKGYIQANYRYVSKNYIESNVNFEDDLSLITQHGIYQAGLLSEYNLGRNWALKGQYFHDTKEDQMIEALVGVTYLSDC
WSFGLTYSDQLIAPESAKTIGTYEPEYESNLMLSIAIRGLGNNTGITSGSANNALDYGRPFYLNN

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family

Homologues:

Organism=Escherichia coli, GI1786239, Length=729, Percent_Identity=37.5857338820302, Blast_Score=464, Evalue=1e-132,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LPTD_VIBF1 (Q5E862)

Other databases:

- EMBL:   CP000020
- RefSeq:   YP_203672.1
- ProteinModelPortal:   Q5E862
- STRING:   Q5E862
- GeneID:   3277243
- GenomeReviews:   CP000020_GR
- KEGG:   vfi:VF_0289
- NMPDR:   fig|312309.3.peg.289
- eggNOG:   COG1452
- HOGENOM:   HBG563511
- OMA:   TPYYWNI
- PhylomeDB:   Q5E862
- ProtClustDB:   PRK03761
- BioCyc:   VFIS312309:VF0289-MONOMER
- HAMAP:   MF_01411
- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543

Pfam domain/function: PF03968 OstA; PF04453 OstA_C

EC number: NA

Molecular weight: Translated: 89297; Mature: 89166

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTSRSLLASFTGCLLYGTPAIADNGSEPVSPENGIVFPLQTDAVNSCEVPDKKSKEEE
CCCCHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
DQQPVLIQADSVEASNNSKAVYKGNVHIIKGRQEIKADSITLHQQENIAIAEGNVDYSSM
CCCCEEEEECCEECCCCCCEEEECCEEEEECCHHCCCCEEEEEECCCEEEEECCCCCCEE
EMRTTSDKVTTNLSTEDTTMVNTAYKMSCQPIRGQAGRVLKTGQEIYQLEDASFTTCPAD
EEEECCCCEEEECCCCCCEEEEEEEEEECCCCCCCCCCHHHCCCHHEEECCCCEEECCCC
DNSWRFKASDIELDQSDEWATFYNARFEVLDVPIFYLPYVTVPVGDTRKTGVLIPSIGLD
CCCEEEEECCCCCCCCCCEEEEECCEEEEEECCEEEECEEEEECCCCCCCCEEEECCCCC
SKNGFELSVPIYWNIAPNYDATTTINYMERRGTQLETEFRYLTELGKGTVDAEYLNEDDK
CCCCEEEEEEEEEEECCCCCCCEEHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCCCCC
FKDKGSRWGVSWDHSGIYQQHWKFDVEYSKVSDIDYFQDLNSSIGTRDEGQLQQSGEVSY
HHCCCCCCCCCCCCCCCCHHHCEEEEEHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEE
RSQDWDMTMRVRDFQVLVEEQTPYRLMPQIEFNYYAPQFYSEFDFNLHSHISKFTTDDKA
ECCCCCEEEEEEEEEEEEECCCCEEECCEEEEEEECCHHHHHCCCCHHHHHHHHCCCCCC
KPSATRVHLEPKLSLPLSGTWWSLIPETSLLYTYYQQDFDKQPVGPNGSLNLDHEVSRTI
CCCCEEEEECCCCCCCCCCCHHHHCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCHHHHCC
PEVRINGAIYLDSTHKFLGEYLQTLEPKIQYLYVPEVDQSNIYGGTGDGGYDSSKLQLDY
CCEEECCEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEE
YGLFRDRQYSGVDYIADANQFSVGATSRFYDDAYKERMNISFGQILYLNGSGTEQNNDDK
EEEEECCCCCCCCEEECCCCEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
NSSAWAMESDFNYDDYLFYHGGIQYDSNVSELQVANSTLEYRFSKGYIQANYRYVSKNYI
CCCEEEEECCCCCCCEEEEECCEEECCCCHHEEEECCEEEEEEECCEEEEEEEEEECHHH
ESNVNFEDDLSLITQHGIYQAGLLSEYNLGRNWALKGQYFHDTKEDQMIEALVGVTYLSD
HCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHH
CWSFGLTYSDQLIAPESAKTIGTYEPEYESNLMLSIAIRGLGNNTGITSGSANNALDYGR
HHHHCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCC
PFYLNN
CEEECC
>Mature Secondary Structure 
SFTSRSLLASFTGCLLYGTPAIADNGSEPVSPENGIVFPLQTDAVNSCEVPDKKSKEEE
CCCHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
DQQPVLIQADSVEASNNSKAVYKGNVHIIKGRQEIKADSITLHQQENIAIAEGNVDYSSM
CCCCEEEEECCEECCCCCCEEEECCEEEEECCHHCCCCEEEEEECCCEEEEECCCCCCEE
EMRTTSDKVTTNLSTEDTTMVNTAYKMSCQPIRGQAGRVLKTGQEIYQLEDASFTTCPAD
EEEECCCCEEEECCCCCCEEEEEEEEEECCCCCCCCCCHHHCCCHHEEECCCCEEECCCC
DNSWRFKASDIELDQSDEWATFYNARFEVLDVPIFYLPYVTVPVGDTRKTGVLIPSIGLD
CCCEEEEECCCCCCCCCCEEEEECCEEEEEECCEEEECEEEEECCCCCCCCEEEECCCCC
SKNGFELSVPIYWNIAPNYDATTTINYMERRGTQLETEFRYLTELGKGTVDAEYLNEDDK
CCCCEEEEEEEEEEECCCCCCCEEHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCCCCC
FKDKGSRWGVSWDHSGIYQQHWKFDVEYSKVSDIDYFQDLNSSIGTRDEGQLQQSGEVSY
HHCCCCCCCCCCCCCCCCHHHCEEEEEHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEE
RSQDWDMTMRVRDFQVLVEEQTPYRLMPQIEFNYYAPQFYSEFDFNLHSHISKFTTDDKA
ECCCCCEEEEEEEEEEEEECCCCEEECCEEEEEEECCHHHHHCCCCHHHHHHHHCCCCCC
KPSATRVHLEPKLSLPLSGTWWSLIPETSLLYTYYQQDFDKQPVGPNGSLNLDHEVSRTI
CCCCEEEEECCCCCCCCCCCHHHHCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCHHHHCC
PEVRINGAIYLDSTHKFLGEYLQTLEPKIQYLYVPEVDQSNIYGGTGDGGYDSSKLQLDY
CCEEECCEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEE
YGLFRDRQYSGVDYIADANQFSVGATSRFYDDAYKERMNISFGQILYLNGSGTEQNNDDK
EEEEECCCCCCCCEEECCCCEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
NSSAWAMESDFNYDDYLFYHGGIQYDSNVSELQVANSTLEYRFSKGYIQANYRYVSKNYI
CCCEEEEECCCCCCCEEEEECCEEECCCCHHEEEECCEEEEEEECCEEEEEEEEEECHHH
ESNVNFEDDLSLITQHGIYQAGLLSEYNLGRNWALKGQYFHDTKEDQMIEALVGVTYLSD
HCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHH
CWSFGLTYSDQLIAPESAKTIGTYEPEYESNLMLSIAIRGLGNNTGITSGSANNALDYGR
HHHHCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCC
PFYLNN
CEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA