Click here to switch to the map view.

The map label for this gene is mdh [H]

Identifier: 59710883

GI number: 59710883

Start: 283104

End: 284039

Strand: Direct

Name: mdh [H]

Synonym: VF_0276

Alternate gene names: 59710883

Gene position: 283104-284039 (Clockwise)

Preceding gene: 59710878

Following gene: 59710885

Centisome position: 9.77

GC content: 41.88

Gene sequence:

>936_bases
ATGAAAGTAGCTGTTATTGGTGCCGCTGGCGGCATTGGACAAGCGTTAGCATTACTTCTTAAGAACCGTTTACCAGCAGG
GTCAGATCTAGCTTTATATGATATTGCTCCAGTAACTCCAGGTGTTGCTGCGGATCTAAGCCATATTCCAACACCAGTAT
CAATCAAAGGATATTGCGGAGAAGATCCAACACCAGCATTAGAAGGTGCAGATGTTGTACTTATTTCTGCAGGTGTTGCT
CGTAAACCTGGAATGGATCGTTCTGATCTTTTTAATATCAATGCAGGTATTGTTAAATCGTTAACTGAAAAGATTGCGGT
AACTTGTCCTAAAGCGTGTATTGGTATTATTACAAACCCTGTAAATACTACGGTAGCAATTGCTGCAGAAGTATTAAAAA
AAGCAGGTGTGTATGACAAAAATAAATTATTTGGTGTAACCACGCTGGATGTGATCCGTTCAGAGACTTTCGTTGCTGAA
TTAAAAGATAAAGATCCAGGTGAGATCCGTGTTCCTGTTATTGGTGGTCACTCTGGTGTCACAATCCTTCCTTTATTATC
TCAAGTTCAAGGTGTTGAGTTTACAGCTGAAGAAGTGGCTGCACTGACTCCTCGCATCCAAAACGCGGGTACAGAAGTGG
TTGAAGCAAAAGCGGGTGGCGGCTCAGCAACGTTGTCTATGGGGCAAGCGGCATGTCGTTTTGGTCTATCACTAGTAAAA
GCACTGAGTGGTGAAGAAGGCGTTGTTGAATGTGCTTATGTTGAAGGTAACGGTGAACATGCTCGTTTCTTCGCACAACC
AATTCTACTTGGCAAAAATGGGGTTGAAGAAATTCAATCTTATGGTGAGTTAAGTGCATTTGAACAAGAAGCGCTAGAAA
GTATGCTAGATACGCTAAGAGGCGATATCAAAATTGGCGAAGAGTTTGTTCAATAA

Upstream 100 bases:

>100_bases
TTCGTTGAATTTCACCTGTCCTTACTATAACGTCAGATTCAGTCTGAAAGTAAAATCTTTAAATAATAACAATATTCCTA
CCATTTCAAGGAGAACGAAA

Downstream 100 bases:

>100_bases
GTGTTAACTTAGACATTAATTGAAAGAATAAAGGGGCAGATAGTCTAACTATCTGCCCCTTTATTATGTTTGAAAGAAAA
GGTGTGGATTATTTACTGCG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYCGEDPTPALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVKSLTEKIAVTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDVIRSETFVAE
LKDKDPGEIRVPVIGGHSGVTILPLLSQVQGVEFTAEEVAALTPRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVK
ALSGEEGVVECAYVEGNGEHARFFAQPILLGKNGVEEIQSYGELSAFEQEALESMLDTLRGDIKIGEEFVQ

Sequences:

>Translated_311_residues
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYCGEDPTPALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVKSLTEKIAVTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDVIRSETFVAE
LKDKDPGEIRVPVIGGHSGVTILPLLSQVQGVEFTAEEVAALTPRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVK
ALSGEEGVVECAYVEGNGEHARFFAQPILLGKNGVEEIQSYGELSAFEQEALESMLDTLRGDIKIGEEFVQ
>Mature_311_residues
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYCGEDPTPALEGADVVLISAGVA
RKPGMDRSDLFNINAGIVKSLTEKIAVTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDVIRSETFVAE
LKDKDPGEIRVPVIGGHSGVTILPLLSQVQGVEFTAEEVAALTPRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVK
ALSGEEGVVECAYVEGNGEHARFFAQPILLGKNGVEEIQSYGELSAFEQEALESMLDTLRGDIKIGEEFVQ

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 1 family [H]

Homologues:

Organism=Homo sapiens, GI21735621, Length=312, Percent_Identity=57.3717948717949, Blast_Score=340, Evalue=8e-94,
Organism=Escherichia coli, GI1789632, Length=311, Percent_Identity=83.6012861736334, Blast_Score=524, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17554310, Length=313, Percent_Identity=55.591054313099, Blast_Score=320, Evalue=4e-88,
Organism=Saccharomyces cerevisiae, GI6322765, Length=315, Percent_Identity=49.2063492063492, Blast_Score=265, Evalue=7e-72,
Organism=Saccharomyces cerevisiae, GI6320125, Length=338, Percent_Identity=39.3491124260355, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6324446, Length=363, Percent_Identity=36.9146005509642, Blast_Score=181, Evalue=9e-47,
Organism=Drosophila melanogaster, GI24647881, Length=310, Percent_Identity=56.1290322580645, Blast_Score=326, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24663599, Length=318, Percent_Identity=49.3710691823899, Blast_Score=271, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24663595, Length=300, Percent_Identity=44.3333333333333, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010097
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 32252; Mature: 32252

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00068 MDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYCG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCEEECCCC
EDPTPALEGADVVLISAGVARKPGMDRSDLFNINAGIVKSLTEKIAVTCPKACIGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCCHHHHHHHCCC
VNTTVAIAAEVLKKAGVYDKNKLFGVTTLDVIRSETFVAELKDKDPGEIRVPVIGGHSGV
CHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHEEECCCCCCCCEEEEEEECCCCC
TILPLLSQVQGVEFTAEEVAALTPRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVK
HHHHHHHHHCCCCCCHHHHHHHCCHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
ALSGEEGVVECAYVEGNGEHARFFAQPILLGKNGVEEIQSYGELSAFEQEALESMLDTLR
HHCCCCCCEEEEEEECCCCEEEEECCCCEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHH
GDIKIGEEFVQ
CCHHHHHHHCC
>Mature Secondary Structure
MKVAVIGAAGGIGQALALLLKNRLPAGSDLALYDIAPVTPGVAADLSHIPTPVSIKGYCG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCEEECCCC
EDPTPALEGADVVLISAGVARKPGMDRSDLFNINAGIVKSLTEKIAVTCPKACIGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHCCHHHHHHHCCC
VNTTVAIAAEVLKKAGVYDKNKLFGVTTLDVIRSETFVAELKDKDPGEIRVPVIGGHSGV
CHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHEEECCCCCCCCEEEEEEECCCCC
TILPLLSQVQGVEFTAEEVAALTPRIQNAGTEVVEAKAGGGSATLSMGQAACRFGLSLVK
HHHHHHHHHCCCCCCHHHHHHHCCHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
ALSGEEGVVECAYVEGNGEHARFFAQPILLGKNGVEEIQSYGELSAFEQEALESMLDTLR
HHCCCCCCEEEEEEECCCCEEEEECCCCEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHH
GDIKIGEEFVQ
CCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA