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The map label for this gene is mpl [H]

Identifier: 59710872

GI number: 59710872

Start: 268157

End: 269536

Strand: Direct

Name: mpl [H]

Synonym: VF_0265

Alternate gene names: 59710872

Gene position: 268157-269536 (Clockwise)

Preceding gene: 59710870

Following gene: 59710873

Centisome position: 9.25

GC content: 40.87

Gene sequence:

>1380_bases
ATGCATATTCATATCCTTGGAATTTGTGGAACATTCATGGGTGGTGCTGCAACACTAGCACGTCAACTCGGACATAAAGT
AACTGGTTGTGATGCTAACGTTTATCCACCAATGAGCACATTATTAGAGTCTCAAGGCATTGAGATTATTGAAGGCTTTG
ATCCAAGCCAGTTAGACCCTGCACCTGATTTAGTCGTCATTGGTAATGCCATGAGTCGTGGAAATCCATGTGTTGAATAT
GTATTAGATAAGAATCTTAAATACACATCAGGTCCTCAATGGTTACAAGAGTTTTTACTGCAAGATCGTTGGGTGATGGC
GGTTGCAGGTACACACGGTAAAACAACAACATCAAGTATGTTAGCTTGGGTACTAGAAGAGTGTGGTTACAAGCCAGGCT
TCTTAGTCGGCGGTGTATTAGGTAATTTTGGTGAGTCAGCTCGCTTAGGCGAAAGCATGTTCTTTGTTGTTGAAGCAGAT
GAATATGACAGTGCTTTTTTTGATAAACGTTCAAAGTTTGTTCATTACCGTCCTCGTACGCTTGTATTAAACAATCTTGA
GTTTGACCATGCCGATATTTTTGATGACTTGGCAGCGATTCAGCGTCAATTCCATCACTTAATCAGAACGGTTCCAAGTA
CTGGTCGCATTTTTGCACCTAAGCAAGATAAACCTTTAGAGCAAGTATTAGAACAAGGCTGCTGGAGTGAATTAGAGTTC
ACTGGTGAAGATGGTCAATGGAAAGCCATCAAACTTCAAAAAGATGGTAGTCAGTTTGATGTCTATTTTGAGGGTGAAAA
CGTTGGGCAAGTTAATTGGGACTTAGTTGGCGATCACAACGTAAGTAATGCGCTTATGGTCGTCGCTTCTGCACGCCACG
TAGGTGTTACTCCTGATTTAGCTTGTGAAGCTTTGGGTAAATTTATCAATACTAAGCGTCGTCTTGAGTTGAAAGGCGAA
GTAAATGGTGTGACGGTTTATGATGACTTTGCTCATCACCCAACAGCTATTGATCTAACATTAAATGGTCTACGTAATAA
AGTTGGTGATAAAAAGATTATTGCCGTATTAGAACCTCGCTCAAGCACAATGAAGCTTGGCGTTCATAAAGATACATTAG
CGGCTTCACTGCATGCCGCAGATTCAGTTTATCTCTATCAACCAGATAATATCGCATGGTCTGTTGATGACGTTGCACAA
CAATGTACTCAACCTGCTCACACGCATAATAACATTGATGCGTTCGTTGATATGATAGTGAACGAAGCACAGCCTGGTGA
TCAAATTTTGGTAATGAGCAATGGTGGCTTTGAAGGTATTCACGGTAAATTGCTTTCAGGCTTAGGTGATGCCGTAAAAC
ATATAGAAATGGTGGATTAA

Upstream 100 bases:

>100_bases
GTTTGCTGTAATTCATTCTATAGCATAGAACTTTTTAAGAGTATTGAAGTAACTTTGTATAAAAGTTGCTCACTTAATTT
AATATAAGACGGTTTTTATT

Downstream 100 bases:

>100_bases
ACATGAGTAAACAAAAAGCAATTACGTTAGCTATGACAGGGGCTTCAGGAGCGCCTTATGGATTGCGTTTGCTCGAGTGT
TTACTTGCTGCTGATTATCA

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MHIHILGICGTFMGGAATLARQLGHKVTGCDANVYPPMSTLLESQGIEIIEGFDPSQLDPAPDLVVIGNAMSRGNPCVEY
VLDKNLKYTSGPQWLQEFLLQDRWVMAVAGTHGKTTTSSMLAWVLEECGYKPGFLVGGVLGNFGESARLGESMFFVVEAD
EYDSAFFDKRSKFVHYRPRTLVLNNLEFDHADIFDDLAAIQRQFHHLIRTVPSTGRIFAPKQDKPLEQVLEQGCWSELEF
TGEDGQWKAIKLQKDGSQFDVYFEGENVGQVNWDLVGDHNVSNALMVVASARHVGVTPDLACEALGKFINTKRRLELKGE
VNGVTVYDDFAHHPTAIDLTLNGLRNKVGDKKIIAVLEPRSSTMKLGVHKDTLAASLHAADSVYLYQPDNIAWSVDDVAQ
QCTQPAHTHNNIDAFVDMIVNEAQPGDQILVMSNGGFEGIHGKLLSGLGDAVKHIEMVD

Sequences:

>Translated_459_residues
MHIHILGICGTFMGGAATLARQLGHKVTGCDANVYPPMSTLLESQGIEIIEGFDPSQLDPAPDLVVIGNAMSRGNPCVEY
VLDKNLKYTSGPQWLQEFLLQDRWVMAVAGTHGKTTTSSMLAWVLEECGYKPGFLVGGVLGNFGESARLGESMFFVVEAD
EYDSAFFDKRSKFVHYRPRTLVLNNLEFDHADIFDDLAAIQRQFHHLIRTVPSTGRIFAPKQDKPLEQVLEQGCWSELEF
TGEDGQWKAIKLQKDGSQFDVYFEGENVGQVNWDLVGDHNVSNALMVVASARHVGVTPDLACEALGKFINTKRRLELKGE
VNGVTVYDDFAHHPTAIDLTLNGLRNKVGDKKIIAVLEPRSSTMKLGVHKDTLAASLHAADSVYLYQPDNIAWSVDDVAQ
QCTQPAHTHNNIDAFVDMIVNEAQPGDQILVMSNGGFEGIHGKLLSGLGDAVKHIEMVD
>Mature_459_residues
MHIHILGICGTFMGGAATLARQLGHKVTGCDANVYPPMSTLLESQGIEIIEGFDPSQLDPAPDLVVIGNAMSRGNPCVEY
VLDKNLKYTSGPQWLQEFLLQDRWVMAVAGTHGKTTTSSMLAWVLEECGYKPGFLVGGVLGNFGESARLGESMFFVVEAD
EYDSAFFDKRSKFVHYRPRTLVLNNLEFDHADIFDDLAAIQRQFHHLIRTVPSTGRIFAPKQDKPLEQVLEQGCWSELEF
TGEDGQWKAIKLQKDGSQFDVYFEGENVGQVNWDLVGDHNVSNALMVVASARHVGVTPDLACEALGKFINTKRRLELKGE
VNGVTVYDDFAHHPTAIDLTLNGLRNKVGDKKIIAVLEPRSSTMKLGVHKDTLAASLHAADSVYLYQPDNIAWSVDDVAQ
QCTQPAHTHNNIDAFVDMIVNEAQPGDQILVMSNGGFEGIHGKLLSGLGDAVKHIEMVD

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=449, Percent_Identity=69.0423162583519, Blast_Score=657, Evalue=0.0,
Organism=Escherichia coli, GI1786279, Length=326, Percent_Identity=28.5276073619632, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50508; Mature: 50508

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGAATLARQLGHKVTGCDANVYPPMSTLLESQGIEIIEGFDPSQLDP
CEEEEEEEHHHHCCHHHHHHHHHCCEEECCCCCCCCCHHHHHHHCCCEEEECCCCCCCCC
APDLVVIGNAMSRGNPCVEYVLDKNLKYTSGPQWLQEFLLQDRWVMAVAGTHGKTTTSSM
CCCEEEEECCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWVLEECGYKPGFLVGGVLGNFGESARLGESMFFVVEADEYDSAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEEHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHCCCCEEEECCCE
LVLNNLEFDHADIFDDLAAIQRQFHHLIRTVPSTGRIFAPKQDKPLEQVLEQGCWSELEF
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCCCCEEE
TGEDGQWKAIKLQKDGSQFDVYFEGENVGQVNWDLVGDHNVSNALMVVASARHVGVTPDL
ECCCCCEEEEEEECCCCEEEEEECCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCHHH
ACEALGKFINTKRRLELKGEVNGVTVYDDFAHHPTAIDLTLNGLRNKVGDKKIIAVLEPR
HHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCEEEEEECHHHHCCCCCEEEEEECCC
SSTMKLGVHKDTLAASLHAADSVYLYQPDNIAWSVDDVAQQCTQPAHTHNNIDAFVDMIV
CCEEEECCCHHHHHHHHHCCCEEEEECCCCCEECHHHHHHHCCCCCCCCCCHHHHHHHHH
NEAQPGDQILVMSNGGFEGIHGKLLSGLGDAVKHIEMVD
HCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHIHILGICGTFMGGAATLARQLGHKVTGCDANVYPPMSTLLESQGIEIIEGFDPSQLDP
CEEEEEEEHHHHCCHHHHHHHHHCCEEECCCCCCCCCHHHHHHHCCCEEEECCCCCCCCC
APDLVVIGNAMSRGNPCVEYVLDKNLKYTSGPQWLQEFLLQDRWVMAVAGTHGKTTTSSM
CCCEEEEECCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWVLEECGYKPGFLVGGVLGNFGESARLGESMFFVVEADEYDSAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEEHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHCCCCEEEECCCE
LVLNNLEFDHADIFDDLAAIQRQFHHLIRTVPSTGRIFAPKQDKPLEQVLEQGCWSELEF
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCCCCEEE
TGEDGQWKAIKLQKDGSQFDVYFEGENVGQVNWDLVGDHNVSNALMVVASARHVGVTPDL
ECCCCCEEEEEEECCCCEEEEEECCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCHHH
ACEALGKFINTKRRLELKGEVNGVTVYDDFAHHPTAIDLTLNGLRNKVGDKKIIAVLEPR
HHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCEEEEEECHHHHCCCCCEEEEEECCC
SSTMKLGVHKDTLAASLHAADSVYLYQPDNIAWSVDDVAQQCTQPAHTHNNIDAFVDMIV
CCEEEECCCHHHHHHHHHCCCEEEEECCCCCEECHHHHHHHCCCCCCCCCCHHHHHHHHH
NEAQPGDQILVMSNGGFEGIHGKLLSGLGDAVKHIEMVD
HCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]