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The map label for this gene is rfaF [H]

Identifier: 59710746

GI number: 59710746

Start: 157441

End: 158466

Strand: Reverse

Name: rfaF [H]

Synonym: VF_0139

Alternate gene names: 59710746

Gene position: 158466-157441 (Counterclockwise)

Preceding gene: 59710757

Following gene: 59710745

Centisome position: 5.47

GC content: 40.35

Gene sequence:

>1026_bases
ATGAAAATACTAATCATTGGCCCATCATGGGTGGGTGATATGGTGATGTCCCAATCACTCTATATCACACTAAAACAACA
ACATCCTAAAGCCATTATTGATGTAATGGCACCAGCGTGGTGTAAACCTATTCTAGATCGTATGCCTGAAGTGAATCAAG
CAATTGAAATGCCTCTTGGTCATGGTGATTTTAATCTGTTAGGTCGTCGTGCTCTTGGTAAAGAGTTAAAAAATAATAAC
TATACACACGCTTACATTTGCCCGAACTCTGCAAAATCAGCACTTATTCCTTGGTTTGCAGGGGTCCCGGTTCGTACGGG
TTGGAAAGGTGAAATGCGTTATGGGCTATTAAACGATTTACGTCCAAATAAAAAAGCATTCCAATACATGGTTGAGCGCT
ATGTGGCATTAGCTCATCCTAAATCTGAAATGATTGATTCAAGCTCACTAGGTGGGTTAGAGAATTTACCTCGCCCAGCT
CTATCAATTGATGCGAAAGTTCAAAAAAATACTCTAGAGAAGTTTTCTTTATCTCTTGATAAACCTATTCTTGGTTTGTG
CCCGGGCGCTGAATTTGGTCCTGCAAAACAGTGGCCTGTTGAGCATTATGCAGCCGTTGCTAACGAAATGATCAACCGTG
GCCATCAAGTATGGTTATTTGGCTCTGCCAAAGATAAAGAAACAACTGAAAGTATTAAAGCTTTAGTGGATGCCGATAAC
CAAGCAGCGGTATTTAACCTAGCTGGAGATACAAGCTTAATTGAAGCTGTTGATCTACTTGCCGCTTGTAAAACCGTTGT
AAGTAACGATTCTGGTTTAATGCACGTTGCTGCTGCGGTTGGTTGTCATGTGGTTGGTGTTTATGGTTCAACATCACCAA
AATACACGCCACCATTAGCGGAAAAAGTCGATATTGTAAGCACTGATATTGAGTGTCGCCCTTGCTTTAAGCGTGATTGC
CCTCTTGGTCATTTAAAGTGCTTGAAAGAGTTAAAACCGATTTTAGTACTAAATAAATTATTATGA

Upstream 100 bases:

>100_bases
TGACAGAATTGAGTTCATATAACTTCCTAAGCGTTTTAATTTTAATTAAAGTATACTACTACTTTACCATAACCAACCAT
TGATTTTCTGGATACGCATA

Downstream 100 bases:

>100_bases
TTCGTTTAATTTATTCTTTATTTTTAGCTTTATTATCCCCTTTGCTTCTCTATTCTCTTTATAAGAAAAAAGATGGAAAG
CCTGCTTTTGGACGACGATG

Product: ADP-heptose:LPS heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKILIIGPSWVGDMVMSQSLYITLKQQHPKAIIDVMAPAWCKPILDRMPEVNQAIEMPLGHGDFNLLGRRALGKELKNNN
YTHAYICPNSAKSALIPWFAGVPVRTGWKGEMRYGLLNDLRPNKKAFQYMVERYVALAHPKSEMIDSSSLGGLENLPRPA
LSIDAKVQKNTLEKFSLSLDKPILGLCPGAEFGPAKQWPVEHYAAVANEMINRGHQVWLFGSAKDKETTESIKALVDADN
QAAVFNLAGDTSLIEAVDLLAACKTVVSNDSGLMHVAAAVGCHVVGVYGSTSPKYTPPLAEKVDIVSTDIECRPCFKRDC
PLGHLKCLKELKPILVLNKLL

Sequences:

>Translated_341_residues
MKILIIGPSWVGDMVMSQSLYITLKQQHPKAIIDVMAPAWCKPILDRMPEVNQAIEMPLGHGDFNLLGRRALGKELKNNN
YTHAYICPNSAKSALIPWFAGVPVRTGWKGEMRYGLLNDLRPNKKAFQYMVERYVALAHPKSEMIDSSSLGGLENLPRPA
LSIDAKVQKNTLEKFSLSLDKPILGLCPGAEFGPAKQWPVEHYAAVANEMINRGHQVWLFGSAKDKETTESIKALVDADN
QAAVFNLAGDTSLIEAVDLLAACKTVVSNDSGLMHVAAAVGCHVVGVYGSTSPKYTPPLAEKVDIVSTDIECRPCFKRDC
PLGHLKCLKELKPILVLNKLL
>Mature_341_residues
MKILIIGPSWVGDMVMSQSLYITLKQQHPKAIIDVMAPAWCKPILDRMPEVNQAIEMPLGHGDFNLLGRRALGKELKNNN
YTHAYICPNSAKSALIPWFAGVPVRTGWKGEMRYGLLNDLRPNKKAFQYMVERYVALAHPKSEMIDSSSLGGLENLPRPA
LSIDAKVQKNTLEKFSLSLDKPILGLCPGAEFGPAKQWPVEHYAAVANEMINRGHQVWLFGSAKDKETTESIKALVDADN
QAAVFNLAGDTSLIEAVDLLAACKTVVSNDSGLMHVAAAVGCHVVGVYGSTSPKYTPPLAEKVDIVSTDIECRPCFKRDC
PLGHLKCLKELKPILVLNKLL

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=341, Percent_Identity=53.3724340175953, Blast_Score=364, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 37412; Mature: 37412

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIIGPSWVGDMVMSQSLYITLKQQHPKAIIDVMAPAWCKPILDRMPEVNQAIEMPLG
CEEEEECCCHHHHHHHCCCEEEEEECCCCHHHHHHHCHHHHHHHHHHCCHHHHHHHCCCC
HGDFNLLGRRALGKELKNNNYTHAYICPNSAKSALIPWFAGVPVRTGWKGEMRYGLLNDL
CCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHCC
RPNKKAFQYMVERYVALAHPKSEMIDSSSLGGLENLPRPALSIDAKVQKNTLEKFSLSLD
CCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHCCCCCEEECCHHHHHHHHHHHHCCC
KPILGLCPGAEFGPAKQWPVEHYAAVANEMINRGHQVWLFGSAKDKETTESIKALVDADN
CCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCC
QAAVFNLAGDTSLIEAVDLLAACKTVVSNDSGLMHVAAAVGCHVVGVYGSTSPKYTPPLA
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCEEEEEEECCCCCCCCCCHH
EKVDIVSTDIECRPCFKRDCPLGHLKCLKELKPILVLNKLL
HHCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILIIGPSWVGDMVMSQSLYITLKQQHPKAIIDVMAPAWCKPILDRMPEVNQAIEMPLG
CEEEEECCCHHHHHHHCCCEEEEEECCCCHHHHHHHCHHHHHHHHHHCCHHHHHHHCCCC
HGDFNLLGRRALGKELKNNNYTHAYICPNSAKSALIPWFAGVPVRTGWKGEMRYGLLNDL
CCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHCC
RPNKKAFQYMVERYVALAHPKSEMIDSSSLGGLENLPRPALSIDAKVQKNTLEKFSLSLD
CCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHCCCCCEEECCHHHHHHHHHHHHCCC
KPILGLCPGAEFGPAKQWPVEHYAAVANEMINRGHQVWLFGSAKDKETTESIKALVDADN
CCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCC
QAAVFNLAGDTSLIEAVDLLAACKTVVSNDSGLMHVAAAVGCHVVGVYGSTSPKYTPPLA
CEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCEEEEEEECCCCCCCCCCHH
EKVDIVSTDIECRPCFKRDCPLGHLKCLKELKPILVLNKLL
HHCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]