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The map label for this gene is waaA [H]

Identifier: 59710745

GI number: 59710745

Start: 156188

End: 157444

Strand: Reverse

Name: waaA [H]

Synonym: VF_0138

Alternate gene names: 59710745

Gene position: 157444-156188 (Counterclockwise)

Preceding gene: 59710746

Following gene: 59710742

Centisome position: 5.43

GC content: 38.66

Gene sequence:

>1257_bases
ATGATTCGTTTAATTTATTCTTTATTTTTAGCTTTATTATCCCCTTTGCTTCTCTATTCTCTTTATAAGAAAAAAGATGG
AAAGCCTGCTTTTGGACGACGATGGAAAGAGCACTTTGGTTGCACCCCATCACTCAATACAACGCAGGCTCCAGTATGGA
TTCATGCAGTATCGGTTGGTGAAGCCATTGCTGCTGCTCCTATTATTAAAACATTAAAGAAGCAAACCCCTGAACAACCC
ATTCTTGTCACTACGACGACAAGTACTGGCGCTGAGCAAATTGAAAAGTTAGGTGATTTGGTTGAACACAGATACATGCC
GATTGATTTTTGCTTTGCGGTTCGTGGTTTTTTAAAAGCGACTAAACCTGAAAAGATGCTAATTATGGAAACAGAACTGT
GGCCAAATACACTGCATACCGTTGCCAAATTTGGCATTCCTATTTCAGTATTAAATGCTCGTCTTTCTGAAAAATCCTTT
TTAGGCTACAAAAAAGTACAGCCTATATTTAATCTACTTGGTAAACACCTAACTCATGTTTGTTGCCAATATAAAGATGA
TGCAGATCGTTTCGTTGCTCTTGGTATTCAACCTGAGAAAGTACATGTGACAGGGTCTGTAAAGTTTGACATCGAAATTA
CAGAGCAAATACAAGCCTCAGGGGGTATTCTTCGTAAGCAACTTGGAGAAGATCGTCCTATTTGGATTGCGACCAGTACA
CATAAAGGTGAAGATGAGCAAGTTCTAGCTGCCCATCGTTCTTTGTTGCATAAGATACCTAATGCGTTATTAATTTTAGT
TCCGCGTCACCCCGAGCGCTTTAATTCAGTATTTGAGCTTTGCCAAAGCGCTGATTTTAAAACTGTTAAACGCACTTCAA
ACCAGACATTAACACCAAATTGTCAAATATATCTTGGTGATACGATGGGAGAAATGCTTACGCTCATTGGTGCTTCTGAT
ATCTGCTTTATGGGCGGAAGTTTACTTGGTAATAAAGTGGGTGGTCATAATTTACTTGAGCCTGCTGCACTAGCTAAACC
GTCCATTACTGGCCCAAGTTATTATAATTTCTTAGAGATAGCTGAAACGCTAATCAAAGAAGACGCAACGAAAGTCATCA
TTTCTGCTGAAGAGCTGGCAAACCTATTAGAGCAATTAATGACTCAGCCAGAATATGCCGAGAAAATGGGTAAAAATGCA
CAAAGCTTTATTTCAAAAAACTCTGGTGCTGTCATTAAAACCTTAAATATAGTTTAA

Upstream 100 bases:

>100_bases
CTGATATTGAGTGTCGCCCTTGCTTTAAGCGTGATTGCCCTCTTGGTCATTTAAAGTGCTTGAAAGAGTTAAAACCGATT
TTAGTACTAAATAAATTATT

Downstream 100 bases:

>100_bases
TTAATCGATAATAATTCGACTGTAAGATAGAGCTAATGAATATACAATATATCTACTCAATAATTACATATCTTATTATG
ATCAATACCATAATTTACCT

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: KDO2-lipid IVA; CMP; KDO1-lipid IVA [C]

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MIRLIYSLFLALLSPLLLYSLYKKKDGKPAFGRRWKEHFGCTPSLNTTQAPVWIHAVSVGEAIAAAPIIKTLKKQTPEQP
ILVTTTTSTGAEQIEKLGDLVEHRYMPIDFCFAVRGFLKATKPEKMLIMETELWPNTLHTVAKFGIPISVLNARLSEKSF
LGYKKVQPIFNLLGKHLTHVCCQYKDDADRFVALGIQPEKVHVTGSVKFDIEITEQIQASGGILRKQLGEDRPIWIATST
HKGEDEQVLAAHRSLLHKIPNALLILVPRHPERFNSVFELCQSADFKTVKRTSNQTLTPNCQIYLGDTMGEMLTLIGASD
ICFMGGSLLGNKVGGHNLLEPAALAKPSITGPSYYNFLEIAETLIKEDATKVIISAEELANLLEQLMTQPEYAEKMGKNA
QSFISKNSGAVIKTLNIV

Sequences:

>Translated_418_residues
MIRLIYSLFLALLSPLLLYSLYKKKDGKPAFGRRWKEHFGCTPSLNTTQAPVWIHAVSVGEAIAAAPIIKTLKKQTPEQP
ILVTTTTSTGAEQIEKLGDLVEHRYMPIDFCFAVRGFLKATKPEKMLIMETELWPNTLHTVAKFGIPISVLNARLSEKSF
LGYKKVQPIFNLLGKHLTHVCCQYKDDADRFVALGIQPEKVHVTGSVKFDIEITEQIQASGGILRKQLGEDRPIWIATST
HKGEDEQVLAAHRSLLHKIPNALLILVPRHPERFNSVFELCQSADFKTVKRTSNQTLTPNCQIYLGDTMGEMLTLIGASD
ICFMGGSLLGNKVGGHNLLEPAALAKPSITGPSYYNFLEIAETLIKEDATKVIISAEELANLLEQLMTQPEYAEKMGKNA
QSFISKNSGAVIKTLNIV
>Mature_418_residues
MIRLIYSLFLALLSPLLLYSLYKKKDGKPAFGRRWKEHFGCTPSLNTTQAPVWIHAVSVGEAIAAAPIIKTLKKQTPEQP
ILVTTTTSTGAEQIEKLGDLVEHRYMPIDFCFAVRGFLKATKPEKMLIMETELWPNTLHTVAKFGIPISVLNARLSEKSF
LGYKKVQPIFNLLGKHLTHVCCQYKDDADRFVALGIQPEKVHVTGSVKFDIEITEQIQASGGILRKQLGEDRPIWIATST
HKGEDEQVLAAHRSLLHKIPNALLILVPRHPERFNSVFELCQSADFKTVKRTSNQTLTPNCQIYLGDTMGEMLTLIGASD
ICFMGGSLLGNKVGGHNLLEPAALAKPSITGPSYYNFLEIAETLIKEDATKVIISAEELANLLEQLMTQPEYAEKMGKNA
QSFISKNSGAVIKTLNIV

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=419, Percent_Identity=40.3341288782816, Blast_Score=329, Evalue=3e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 46355; Mature: 46355

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRLIYSLFLALLSPLLLYSLYKKKDGKPAFGRRWKEHFGCTPSLNTTQAPVWIHAVSVG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHH
EAIAAAPIIKTLKKQTPEQPILVTTTTSTGAEQIEKLGDLVEHRYMPIDFCFAVRGFLKA
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
TKPEKMLIMETELWPNTLHTVAKFGIPISVLNARLSEKSFLGYKKVQPIFNLLGKHLTHV
CCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
CCQYKDDADRFVALGIQPEKVHVTGSVKFDIEITEQIQASGGILRKQLGEDRPIWIATST
HHHCCCCCCCEEEECCCCCEEEEEEEEEEEEEEHHHHHHCCCHHHHHCCCCCCEEEEECC
HKGEDEQVLAAHRSLLHKIPNALLILVPRHPERFNSVFELCQSADFKTVKRTSNQTLTPN
CCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCC
CQIYLGDTMGEMLTLIGASDICFMGGSLLGNKVGGHNLLEPAALAKPSITGPSYYNFLEI
CEEEECCHHHHHHHHHCCCHHHHHCCHHHCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHH
AETLIKEDATKVIISAEELANLLEQLMTQPEYAEKMGKNAQSFISKNSGAVIKTLNIV
HHHHHHCCHHHEEEEHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure
MIRLIYSLFLALLSPLLLYSLYKKKDGKPAFGRRWKEHFGCTPSLNTTQAPVWIHAVSVG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHH
EAIAAAPIIKTLKKQTPEQPILVTTTTSTGAEQIEKLGDLVEHRYMPIDFCFAVRGFLKA
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
TKPEKMLIMETELWPNTLHTVAKFGIPISVLNARLSEKSFLGYKKVQPIFNLLGKHLTHV
CCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
CCQYKDDADRFVALGIQPEKVHVTGSVKFDIEITEQIQASGGILRKQLGEDRPIWIATST
HHHCCCCCCCEEEECCCCCEEEEEEEEEEEEEEHHHHHHCCCHHHHHCCCCCCEEEEECC
HKGEDEQVLAAHRSLLHKIPNALLILVPRHPERFNSVFELCQSADFKTVKRTSNQTLTPN
CCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCC
CQIYLGDTMGEMLTLIGASDICFMGGSLLGNKVGGHNLLEPAALAKPSITGPSYYNFLEI
CEEEECCHHHHHHHHHCCCHHHHHCCHHHCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHH
AETLIKEDATKVIISAEELANLLEQLMTQPEYAEKMGKNAQSFISKNSGAVIKTLNIV
HHHHHHCCHHHEEEEHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO1-lipid IVA; CMP-3-deoxy-D-manno-octulosonate; lipid IVA [C]

Specific reaction: KDO1-lipid IVA + CMP-3-deoxy-D-manno-octulosonate = KDO2-lipid IVA + CMP CMP-3-deoxy-D-manno-octulosonate + lipid IVA = KDO1-lipid IVA + CMP [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]