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The map label for this gene is ntrC [H]

Identifier: 59710702

GI number: 59710702

Start: 110973

End: 112376

Strand: Reverse

Name: ntrC [H]

Synonym: VF_0095

Alternate gene names: 59710702

Gene position: 112376-110973 (Counterclockwise)

Preceding gene: 59710703

Following gene: 59710701

Centisome position: 3.88

GC content: 41.03

Gene sequence:

>1404_bases
ATGAGTAAAGGATTTATCTGGGTTGTTGATGATGACAGCTCAATTCGCTGGGTATTAGAAAAGACCTTAACTAGCACCAA
TATGATGTGTGAAAGTTTTGGTGATGCTGAAAGTGTCATTCAAGCACTAGAGCGCAATGTCCCTGATGTGATTATTTCAG
ATATTCGTATGCCGGGAATGGATGGCTTATCGCTTCTTCATCATATTCAAGAAAATTACCCAGAATTACCTGTGATTATC
ATGACGGCACACTCAGACTTAGATGCGGCTGTCAGTGCTTATCAAAAAGGGGCGTTTGAATATCTCCCTAAACCTTTTGA
TATTGATGAAACCCTTACGTTAGTAGAGCGTGCTTTAGCGCACAGCCAAGAACAAAAAAAACAGAGCATCAATACTCCGA
AAAAGGAGCATCATGTTCCCGAGATCATTGGCGAAGCACCCGCGATGCAAGAGGTATTTAGAGCTATAGGACGCTTATCT
CGCTCATCAATATCCGTTTTAATTAATGGTGAATCTGGTACTGGTAAAGAATTGGTTGCCCACGCACTACACCGACATAG
CCCAAGAGCAAATAATGAATTTATCGCACTTAACATGGCAGCAATTCCTAAAGATTTAATTGAATCGGAACTGTTTGGTC
ATGAGAAAGGCGCATTTACAGGTGCAAACTCCGTTCGAAAAGGTCGCTTTGAGCAAGCTAATGGCGGTACGCTGTTTTTA
GATGAAATTGGAGACATGCCATTAGATATTCAAACCAGATTACTGCGTGTTCTTGCTGATGGGCAATTTTATCGTGTTGG
TGGGCACTCTCCGGTTCAAGTGGATGTTCGTATCATTGCAGCTACTCACCAAAATCTTGAGAAACTAGTGACTGATGGTG
AATTTAGAGAAGATTTATTCCATCGCCTAAACGTCATTAGAGTTCATATTCCCGCCTTAAGAGAAAGGCGTGAAGACATA
AAGAAACTCGCAGAACATTTCTTACGCTTAGCCGCAAAAGAGCTGGCTGTCGAATGTAAAACACTAAGCCAAAGTGCCAG
TGATAAATTAAGCCAATGCGACTGGCCTGGTAATGTCCGTCAACTAGAAAATACCTGTCGTTGGTTAACGGTAATGGCAA
GCAGCCAAGAAGTGTTGCCTTCTGATTTACCGCCAGAATTATTCTCAACACCGATACCGCAACAGCAGCATCAAATCACT
GATTGGCAAGAACAACTCAGTTCATGGGTAGAAAATCAATTGAATAAAGGTGAAGAAGATATTCTAAATAAAGTGATCCC
AAATGTTGAACGTATCTTATTAGATAAAGCTCTGCATCATACGCATGGACATAAACAAGATGCCGCAAAACTTCTTGGTT
GGGGAAGAAATACACTAACAAGGAAATTAAAAGAGTTGGAATAA

Upstream 100 bases:

>100_bases
AAATAGATGTCTCTAGCTGGCCGGGAAAAACCATTTTCACCATTTACCTCCCTCTCAAATAAAAATAACCTCACAAAAGG
GATAACGGAGAAGCAAAATT

Downstream 100 bases:

>100_bases
AACTGAAGATTCTGTAATGGCTGCCGATATATCTATAATAAGCAAATTGAGTTATTTAGATAATATCGGTTGCACCATTT
ATCTAAAAAAACACAGAACA

Product: fused DNA-binding response regulator in two-component regulatory system with NtrB: nitrogen regulatory response regulator/sigma54 interaction protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSKGFIWVVDDDSSIRWVLEKTLTSTNMMCESFGDAESVIQALERNVPDVIISDIRMPGMDGLSLLHHIQENYPELPVII
MTAHSDLDAAVSAYQKGAFEYLPKPFDIDETLTLVERALAHSQEQKKQSINTPKKEHHVPEIIGEAPAMQEVFRAIGRLS
RSSISVLINGESGTGKELVAHALHRHSPRANNEFIALNMAAIPKDLIESELFGHEKGAFTGANSVRKGRFEQANGGTLFL
DEIGDMPLDIQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQNLEKLVTDGEFREDLFHRLNVIRVHIPALRERREDI
KKLAEHFLRLAAKELAVECKTLSQSASDKLSQCDWPGNVRQLENTCRWLTVMASSQEVLPSDLPPELFSTPIPQQQHQIT
DWQEQLSSWVENQLNKGEEDILNKVIPNVERILLDKALHHTHGHKQDAAKLLGWGRNTLTRKLKELE

Sequences:

>Translated_467_residues
MSKGFIWVVDDDSSIRWVLEKTLTSTNMMCESFGDAESVIQALERNVPDVIISDIRMPGMDGLSLLHHIQENYPELPVII
MTAHSDLDAAVSAYQKGAFEYLPKPFDIDETLTLVERALAHSQEQKKQSINTPKKEHHVPEIIGEAPAMQEVFRAIGRLS
RSSISVLINGESGTGKELVAHALHRHSPRANNEFIALNMAAIPKDLIESELFGHEKGAFTGANSVRKGRFEQANGGTLFL
DEIGDMPLDIQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQNLEKLVTDGEFREDLFHRLNVIRVHIPALRERREDI
KKLAEHFLRLAAKELAVECKTLSQSASDKLSQCDWPGNVRQLENTCRWLTVMASSQEVLPSDLPPELFSTPIPQQQHQIT
DWQEQLSSWVENQLNKGEEDILNKVIPNVERILLDKALHHTHGHKQDAAKLLGWGRNTLTRKLKELE
>Mature_466_residues
SKGFIWVVDDDSSIRWVLEKTLTSTNMMCESFGDAESVIQALERNVPDVIISDIRMPGMDGLSLLHHIQENYPELPVIIM
TAHSDLDAAVSAYQKGAFEYLPKPFDIDETLTLVERALAHSQEQKKQSINTPKKEHHVPEIIGEAPAMQEVFRAIGRLSR
SSISVLINGESGTGKELVAHALHRHSPRANNEFIALNMAAIPKDLIESELFGHEKGAFTGANSVRKGRFEQANGGTLFLD
EIGDMPLDIQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQNLEKLVTDGEFREDLFHRLNVIRVHIPALRERREDIK
KLAEHFLRLAAKELAVECKTLSQSASDKLSQCDWPGNVRQLENTCRWLTVMASSQEVLPSDLPPELFSTPIPQQQHQITD
WQEQLSSWVENQLNKGEEDILNKVIPNVERILLDKALHHTHGHKQDAAKLLGWGRNTLTRKLKELE

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=471, Percent_Identity=69.0021231422505, Blast_Score=674, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=465, Percent_Identity=40.4301075268817, Blast_Score=343, Evalue=9e-96,
Organism=Escherichia coli, GI1790437, Length=458, Percent_Identity=41.0480349344978, Blast_Score=333, Evalue=9e-93,
Organism=Escherichia coli, GI1788905, Length=370, Percent_Identity=44.0540540540541, Blast_Score=298, Evalue=5e-82,
Organism=Escherichia coli, GI1789233, Length=351, Percent_Identity=42.1652421652422, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1789087, Length=329, Percent_Identity=41.3373860182371, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1786524, Length=347, Percent_Identity=40.0576368876081, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87082117, Length=227, Percent_Identity=48.8986784140969, Blast_Score=231, Evalue=9e-62,
Organism=Escherichia coli, GI87082152, Length=278, Percent_Identity=44.2446043165468, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1787583, Length=325, Percent_Identity=38.1538461538462, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI87081872, Length=226, Percent_Identity=45.575221238938, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI87081858, Length=349, Percent_Identity=30.3724928366762, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789828, Length=226, Percent_Identity=34.5132743362832, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1789809, Length=134, Percent_Identity=30.5970149253731, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1787375, Length=128, Percent_Identity=27.34375, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52616; Mature: 52484

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGFIWVVDDDSSIRWVLEKTLTSTNMMCESFGDAESVIQALERNVPDVIISDIRMPGM
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHCCCCCC
DGLSLLHHIQENYPELPVIIMTAHSDLDAAVSAYQKGAFEYLPKPFDIDETLTLVERALA
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHH
HSQEQKKQSINTPKKEHHVPEIIGEAPAMQEVFRAIGRLSRSSISVLINGESGTGKELVA
HHHHHHHHHCCCCHHHCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
HALHRHSPRANNEFIALNMAAIPKDLIESELFGHEKGAFTGANSVRKGRFEQANGGTLFL
HHHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCEEEH
DEIGDMPLDIQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQNLEKLVTDGEFREDLF
HHHCCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEEHHHHHHHHHHCCCHHHHHHH
HRLNVIRVHIPALRERREDIKKLAEHFLRLAAKELAVECKTLSQSASDKLSQCDWPGNVR
HHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
QLENTCRWLTVMASSQEVLPSDLPPELFSTPIPQQQHQITDWQEQLSSWVENQLNKGEED
HHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
ILNKVIPNVERILLDKALHHTHGHKQDAAKLLGWGRNTLTRKLKELE
HHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
SKGFIWVVDDDSSIRWVLEKTLTSTNMMCESFGDAESVIQALERNVPDVIISDIRMPGM
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHCCCCCC
DGLSLLHHIQENYPELPVIIMTAHSDLDAAVSAYQKGAFEYLPKPFDIDETLTLVERALA
HHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHH
HSQEQKKQSINTPKKEHHVPEIIGEAPAMQEVFRAIGRLSRSSISVLINGESGTGKELVA
HHHHHHHHHCCCCHHHCCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
HALHRHSPRANNEFIALNMAAIPKDLIESELFGHEKGAFTGANSVRKGRFEQANGGTLFL
HHHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCEEEH
DEIGDMPLDIQTRLLRVLADGQFYRVGGHSPVQVDVRIIAATHQNLEKLVTDGEFREDLF
HHHCCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEEHHHHHHHHHHCCCHHHHHHH
HRLNVIRVHIPALRERREDIKKLAEHFLRLAAKELAVECKTLSQSASDKLSQCDWPGNVR
HHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
QLENTCRWLTVMASSQEVLPSDLPPELFSTPIPQQQHQITDWQEQLSSWVENQLNKGEED
HHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
ILNKVIPNVERILLDKALHHTHGHKQDAAKLLGWGRNTLTRKLKELE
HHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]