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The map label for this gene is nudE [H]

Identifier: 172087780

GI number: 172087780

Start: 2761279

End: 2761830

Strand: Reverse

Name: nudE [H]

Synonym: VF_2463

Alternate gene names: 172087780

Gene position: 2761830-2761279 (Counterclockwise)

Preceding gene: 59713071

Following gene: 59713069

Centisome position: 95.32

GC content: 41.12

Gene sequence:

>552_bases
ATGGCTAAAAAACAGTTGCCAACGATTTTGGATACACGCGTTGAAGCCAAATCCAACCTATTCTGCATTGAATCGGTTGA
TTTGCGCTTTTCTAATGGTGTAGAACGTACCTACGAACGAATGAAACCAAGCGGTCGTCATGCTGTCATGATGGTTCCTG
TAACAGAGCAGGGCGATTTACTTTTGGTTCGTGAATATGCTGTTGGAACCGAAAGTTACGAGCTTGGTTTTCCAAAAGGA
TTGATTGATCTAGGAGAGACCCCGTTAGAAGCGGCAAATCGAGAACTAAAAGAAGAAATTGGTTTTGGTGCAAAAACACT
GACGCCATTAAAAGAAATCGTATTAGCGCCTTCCTATTTCTCAAGCAAAATGACATTAATTCTGGCTCAAGATTTATATC
CAGAGCGATTAGAAGGTGATGAGCCTGAACCTTTAGATGTGGTTCGTTGGCCTTTAGCTCAGGCATCAGAGTTATTACAT
CATCTCGATTTTTCAGAGGCAAGATGCATTACCGCACTATTACTTGCTCAACAATTTTTAGCTAAGGAGTAA

Upstream 100 bases:

>100_bases
TTCCCTTCAAGTTTAAGAAGTCAGTTGAAATGGGTAGGCAAGCCTGATTCAAAAGGGCAATATAGAGTGACATACTCAGG
ACGTCTATAAAGGAAAAACG

Downstream 100 bases:

>100_bases
TATTGATGGATTCTACACTTTCTCACCTTATCCCTGAGGTGATCAATATTGCACGTGCATCGGGTCAACTCATTCTCGAT
ATATACGAGAAAGGAGATTT

Product: ADP-ribose diphosphatase

Products: ADP; AMP

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MAKKQLPTILDTRVEAKSNLFCIESVDLRFSNGVERTYERMKPSGRHAVMMVPVTEQGDLLLVREYAVGTESYELGFPKG
LIDLGETPLEAANRELKEEIGFGAKTLTPLKEIVLAPSYFSSKMTLILAQDLYPERLEGDEPEPLDVVRWPLAQASELLH
HLDFSEARCITALLLAQQFLAKE

Sequences:

>Translated_183_residues
MAKKQLPTILDTRVEAKSNLFCIESVDLRFSNGVERTYERMKPSGRHAVMMVPVTEQGDLLLVREYAVGTESYELGFPKG
LIDLGETPLEAANRELKEEIGFGAKTLTPLKEIVLAPSYFSSKMTLILAQDLYPERLEGDEPEPLDVVRWPLAQASELLH
HLDFSEARCITALLLAQQFLAKE
>Mature_182_residues
AKKQLPTILDTRVEAKSNLFCIESVDLRFSNGVERTYERMKPSGRHAVMMVPVTEQGDLLLVREYAVGTESYELGFPKGL
IDLGETPLEAANRELKEEIGFGAKTLTPLKEIVLAPSYFSSKMTLILAQDLYPERLEGDEPEPLDVVRWPLAQASELLHH
LDFSEARCITALLLAQQFLAKE

Specific function: Active on adenosine(5')triphospho(5')adenosine (Ap3A), ADP-ribose, NADH, adenosine(5')diphospho(5')adenosine (Ap2A) [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=176, Percent_Identity=64.7727272727273, Blast_Score=234, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.29; 3.6.1.13; 3.6.1.22

Molecular weight: Translated: 20549; Mature: 20418

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKQLPTILDTRVEAKSNLFCIESVDLRFSNGVERTYERMKPSGRHAVMMVPVTEQGDL
CCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEEEECCCCCE
LLVREYAVGTESYELGFPKGLIDLGETPLEAANRELKEEIGFGAKTLTPLKEIVLAPSYF
EEEEEECCCCCCEECCCCCCHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHH
SSKMTLILAQDLYPERLEGDEPEPLDVVRWPLAQASELLHHLDFSEARCITALLLAQQFL
CCCEEEEEECCCCHHHHCCCCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AKE
HCC
>Mature Secondary Structure 
AKKQLPTILDTRVEAKSNLFCIESVDLRFSNGVERTYERMKPSGRHAVMMVPVTEQGDL
CCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEEEECCCCCE
LLVREYAVGTESYELGFPKGLIDLGETPLEAANRELKEEIGFGAKTLTPLKEIVLAPSYF
EEEEEECCCCCCEECCCCCCHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHH
SSKMTLILAQDLYPERLEGDEPEPLDVVRWPLAQASELLHHLDFSEARCITALLLAQQFL
CCCEEEEEECCCCHHHHCCCCCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AKE
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: P1-P3-bis(5'-adenosyl) triphosphate; H2O

Specific reaction: P1-P3-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9452430 [H]