Click here to switch to the map view.

The map label for this gene is fdnI [H]

Identifier: 172087701

GI number: 172087701

Start: 1501875

End: 1502897

Strand: Reverse

Name: fdnI [H]

Synonym: VF_1358

Alternate gene names: 172087701

Gene position: 1502897-1501875 (Counterclockwise)

Preceding gene: 59711966

Following gene: 59711963

Centisome position: 51.87

GC content: 42.33

Gene sequence:

>1023_bases
ATGTTGAAACAATTAAAACGTCTTTCTCTTTCGTTATTGCTGCCGCTAATGGCAGCATTAACGCTTTCATTTGCTATGCC
AAGCATGGCAAATGAGAGTGCAGCCGCGAAGAGCGCAGAGAAAGAAATGACCCAACTTGCTGGCGCAGATATCTGGCGTG
AAGTAAGAGCAGGTCAAGAAGGCTACACAACTTCGCAATGGCCTGAACATGGCGTACTTATTAGTACGGCAGGCGAAACG
TGGTTTGTACTAAAAGAGAAGTGGATGTCACCATTAGGTGCACTTGCTATTTTTGGTAGTTTAGTAATGGTAACACTGGC
GTACTTCGTCGTTGGTCCATTAAAACTAAGCAAGCCAAAAACAGGCCGTAAGATCAAACGTTGGTCTCGTATGGATCGCG
CCTTACACTGGAGCATGGCATTTAGCTTTTTAACTCTAGCGTTCAGTGGTTTATGTTTAGTTTATGGTAAACACTTTTTA
AAACCGATTTTACCAAGTGAAATCTGGGGCATGATCATTTATGCCGCGAAGCAATACCACAACTACATGGGACCGATCTT
CTTTATCCTACTAATGTGTGTACTTCTGAAATGGTGGAGAAAATCTATCTTCAACAAGACAGACATTCAATGGTTCATGA
AAATGGGCGGCATGGTAGGTAAACACAAAGGTTCACACCCATCAGCAGGTTTCTCTAATGGTGGTGAAAAAGCAATTTAC
TGGTTACTAATTGTATTCGGTATCGTTGTTGCTTTCAGTGGTTTTGTTTTAGATTTTCCAATGTTCGGCCAATTACGTCG
TGACATGGAACTATCTAACCTTGTGCATATGCTATCAGCACTTATCCTTATCTGTGGTTTCATCTTCCACATCTATATCG
GCCTGTTCGGTATGGAAGCGGGTCTAGATGGCATGGTAACCGGTGATGTTGATGAGACATGGGCAAAAGAGCACCATGAC
TTATGGTATCACGAAGTGAAAGACCTTCCTGAAAACCAACCACAAGATAAGAAAGTAGACTAA

Upstream 100 bases:

>100_bases
CGTCAACGTGTTGTTGAGCGTGGCGCGAAAGACGCAGGTTGGACTGACGGTGAAGATCTTGCGTTTGATGCGACTAAGAG
CTAATCCGGTGGAGAGAGTG

Downstream 100 bases:

>100_bases
ATAAGTTAATCACTTTTATTGTCATAAATTCAAAAGCCGAGCAGGGTAACTTGCTCGGTTTTTTTGTGTCTGATGATTGG
TAAAAGAGAAATATTTCTAT

Product: formate dehydrogenase N, gamma subunit

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MLKQLKRLSLSLLLPLMAALTLSFAMPSMANESAAAKSAEKEMTQLAGADIWREVRAGQEGYTTSQWPEHGVLISTAGET
WFVLKEKWMSPLGALAIFGSLVMVTLAYFVVGPLKLSKPKTGRKIKRWSRMDRALHWSMAFSFLTLAFSGLCLVYGKHFL
KPILPSEIWGMIIYAAKQYHNYMGPIFFILLMCVLLKWWRKSIFNKTDIQWFMKMGGMVGKHKGSHPSAGFSNGGEKAIY
WLLIVFGIVVAFSGFVLDFPMFGQLRRDMELSNLVHMLSALILICGFIFHIYIGLFGMEAGLDGMVTGDVDETWAKEHHD
LWYHEVKDLPENQPQDKKVD

Sequences:

>Translated_340_residues
MLKQLKRLSLSLLLPLMAALTLSFAMPSMANESAAAKSAEKEMTQLAGADIWREVRAGQEGYTTSQWPEHGVLISTAGET
WFVLKEKWMSPLGALAIFGSLVMVTLAYFVVGPLKLSKPKTGRKIKRWSRMDRALHWSMAFSFLTLAFSGLCLVYGKHFL
KPILPSEIWGMIIYAAKQYHNYMGPIFFILLMCVLLKWWRKSIFNKTDIQWFMKMGGMVGKHKGSHPSAGFSNGGEKAIY
WLLIVFGIVVAFSGFVLDFPMFGQLRRDMELSNLVHMLSALILICGFIFHIYIGLFGMEAGLDGMVTGDVDETWAKEHHD
LWYHEVKDLPENQPQDKKVD
>Mature_340_residues
MLKQLKRLSLSLLLPLMAALTLSFAMPSMANESAAAKSAEKEMTQLAGADIWREVRAGQEGYTTSQWPEHGVLISTAGET
WFVLKEKWMSPLGALAIFGSLVMVTLAYFVVGPLKLSKPKTGRKIKRWSRMDRALHWSMAFSFLTLAFSGLCLVYGKHFL
KPILPSEIWGMIIYAAKQYHNYMGPIFFILLMCVLLKWWRKSIFNKTDIQWFMKMGGMVGKHKGSHPSAGFSNGGEKAIY
WLLIVFGIVVAFSGFVLDFPMFGQLRRDMELSNLVHMLSALILICGFIFHIYIGLFGMEAGLDGMVTGDVDETWAKEHHD
LWYHEVKDLPENQPQDKKVD

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=222, Percent_Identity=27.4774774774775, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1787750, Length=224, Percent_Identity=24.5535714285714, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 38485; Mature: 38485

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKQLKRLSLSLLLPLMAALTLSFAMPSMANESAAAKSAEKEMTQLAGADIWREVRAGQE
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GYTTSQWPEHGVLISTAGETWFVLKEKWMSPLGALAIFGSLVMVTLAYFVVGPLKLSKPK
CCCCCCCCCCCEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCC
TGRKIKRWSRMDRALHWSMAFSFLTLAFSGLCLVYGKHFLKPILPSEIWGMIIYAAKQYH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
NYMGPIFFILLMCVLLKWWRKSIFNKTDIQWFMKMGGMVGKHKGSHPSAGFSNGGEKAIY
HHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCHHHHH
WLLIVFGIVVAFSGFVLDFPMFGQLRRDMELSNLVHMLSALILICGFIFHIYIGLFGMEA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLDGMVTGDVDETWAKEHHDLWYHEVKDLPENQPQDKKVD
CCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MLKQLKRLSLSLLLPLMAALTLSFAMPSMANESAAAKSAEKEMTQLAGADIWREVRAGQE
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GYTTSQWPEHGVLISTAGETWFVLKEKWMSPLGALAIFGSLVMVTLAYFVVGPLKLSKPK
CCCCCCCCCCCEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCC
TGRKIKRWSRMDRALHWSMAFSFLTLAFSGLCLVYGKHFLKPILPSEIWGMIIYAAKQYH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
NYMGPIFFILLMCVLLKWWRKSIFNKTDIQWFMKMGGMVGKHKGSHPSAGFSNGGEKAIY
HHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCHHHHH
WLLIVFGIVVAFSGFVLDFPMFGQLRRDMELSNLVHMLSALILICGFIFHIYIGLFGMEA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLDGMVTGDVDETWAKEHHDLWYHEVKDLPENQPQDKKVD
CCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]