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The map label for this gene is gltX

Identifier: 58617520

GI number: 58617520

Start: 1270795

End: 1272132

Strand: Reverse

Name: gltX

Synonym: ERGA_CDS_07930

Alternate gene names: 58617520

Gene position: 1272132-1270795 (Counterclockwise)

Preceding gene: 58617522

Following gene: 58617519

Centisome position: 84.81

GC content: 30.94

Gene sequence:

>1338_bases
TTGATTATGATGACGCGTTTTGCTCCAAGTCCTACAGGTTATCTTCATGTGGGGAATGTTAGAACTGCTTTGATATGTTG
GTTATATACTCGTAGTAAACAAGGTAGGTTCTTACTTAGATTTGATGATACTGATCTTCAAAGATCTAAAGATGATTATA
GGAATGAAATCGCTAATGACCTTAAGTGGTTACAGATGGATTGGGATTTTGATGTGAGACAGTCTTCTAGATTTGATCGT
TATGATGAAATATTTAATTACCTATTGAAGGAAGAATTAATATATCCTTGCTATGAGAGTAAAGAGGAGTTGGATTTTAA
AAGGAAAATGCAGTTAAAATTAGGATTGCCACCTATTTATGATAGGAGTGCACTCAAACTTACGCAAGATGAAAAAAATA
AATATTCTGAGCAAGATGTCTATTTTAGGTTTAAGATTGATCAAAGTCAGTTAATAAGTTGGGATGATGAGATACGAGGG
AAGGTATCATTTAATGCGGCAAATATTAGTGATCCAATTGTTAAAAGAGCAGATGGTACCTATACTTATATGTTGCCATC
TGTAATAGATGATATAGATTTTGATATAACTCATATTGTTCGTGGAGAAGATCATATAAGTAATACTGCAATTCAGATAC
AAATGTTTAATGCTCTAAGAGCTAGCGTACCTACGTTTTCACATTTATCTCTTTTATATTGTGATGATAATAAAATCTCG
AAAAGGGTTGGTGGTTTTAGTATTAAAGATATGCAATTTTATGAATTAGAACCTATGGCTATTAATAGTTATTTTGCCAA
GATAGGAACTTCTGATCCAATAGTTGTTCATACAAAAATTCAGGATTTAATATATAATTTTGATATTACAAAGTTTAATC
AAGCTCCTACTCAGTTTAATATAGATGATGTAATAAAACTGAACCCTAAAGTATTACATAAGATGTCTTTTTCAGATGTT
AAACATAGATTAAGTGAGTTAAATATAACTTCTCCAGATTTATGGGATTTTGTTTCTGGAAATGTGCAAAAGTTTTCTGA
TATTCAAGAATGGATAAAAATTTGCGGACAAAGTACTGTACCAGTGATTAATGAATCTGATCAAGATTTTATTAAAATGG
CTTTAAGTGTGTTTCCTAATGGAGAAATCAATCAAGATACTTGGAAAACATGGGTAGCTAATATAAAAGAAAAGACTGAT
CGTAAATCGAAAGATATATTTATTCCTTTAAGATTAGCTTTGACTGGAATATCTACTGGTCCAGAACTTGCAAAATTACT
TCCTATACTTGGTAGAGCAGAAATCATTCGTAGGCTTGGTTATTCTAGTAGATGTTAA

Upstream 100 bases:

>100_bases
TTATTGTATTAAATATATGTTGTTTTATGCTGTGGTTATGGGCTGATGTTACTTGAAAATAATTCTTAATGTAGCAAATA
ATAGGTTTTTTTAAAGGATC

Downstream 100 bases:

>100_bases
TGTATTAAATATGTGGTCAATGAGTTTTTATGGATAGTGTGTTTTTTAATGAATGATGAGCTTATGTAATTTACTGCTTT
GCTTGACGTTGGTTTTGTGA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MIMMTRFAPSPTGYLHVGNVRTALICWLYTRSKQGRFLLRFDDTDLQRSKDDYRNEIANDLKWLQMDWDFDVRQSSRFDR
YDEIFNYLLKEELIYPCYESKEELDFKRKMQLKLGLPPIYDRSALKLTQDEKNKYSEQDVYFRFKIDQSQLISWDDEIRG
KVSFNAANISDPIVKRADGTYTYMLPSVIDDIDFDITHIVRGEDHISNTAIQIQMFNALRASVPTFSHLSLLYCDDNKIS
KRVGGFSIKDMQFYELEPMAINSYFAKIGTSDPIVVHTKIQDLIYNFDITKFNQAPTQFNIDDVIKLNPKVLHKMSFSDV
KHRLSELNITSPDLWDFVSGNVQKFSDIQEWIKICGQSTVPVINESDQDFIKMALSVFPNGEINQDTWKTWVANIKEKTD
RKSKDIFIPLRLALTGISTGPELAKLLPILGRAEIIRRLGYSSRC

Sequences:

>Translated_445_residues
MIMMTRFAPSPTGYLHVGNVRTALICWLYTRSKQGRFLLRFDDTDLQRSKDDYRNEIANDLKWLQMDWDFDVRQSSRFDR
YDEIFNYLLKEELIYPCYESKEELDFKRKMQLKLGLPPIYDRSALKLTQDEKNKYSEQDVYFRFKIDQSQLISWDDEIRG
KVSFNAANISDPIVKRADGTYTYMLPSVIDDIDFDITHIVRGEDHISNTAIQIQMFNALRASVPTFSHLSLLYCDDNKIS
KRVGGFSIKDMQFYELEPMAINSYFAKIGTSDPIVVHTKIQDLIYNFDITKFNQAPTQFNIDDVIKLNPKVLHKMSFSDV
KHRLSELNITSPDLWDFVSGNVQKFSDIQEWIKICGQSTVPVINESDQDFIKMALSVFPNGEINQDTWKTWVANIKEKTD
RKSKDIFIPLRLALTGISTGPELAKLLPILGRAEIIRRLGYSSRC
>Mature_445_residues
MIMMTRFAPSPTGYLHVGNVRTALICWLYTRSKQGRFLLRFDDTDLQRSKDDYRNEIANDLKWLQMDWDFDVRQSSRFDR
YDEIFNYLLKEELIYPCYESKEELDFKRKMQLKLGLPPIYDRSALKLTQDEKNKYSEQDVYFRFKIDQSQLISWDDEIRG
KVSFNAANISDPIVKRADGTYTYMLPSVIDDIDFDITHIVRGEDHISNTAIQIQMFNALRASVPTFSHLSLLYCDDNKIS
KRVGGFSIKDMQFYELEPMAINSYFAKIGTSDPIVVHTKIQDLIYNFDITKFNQAPTQFNIDDVIKLNPKVLHKMSFSDV
KHRLSELNITSPDLWDFVSGNVQKFSDIQEWIKICGQSTVPVINESDQDFIKMALSVFPNGEINQDTWKTWVANIKEKTD
RKSKDIFIPLRLALTGISTGPELAKLLPILGRAEIIRRLGYSSRC

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=480, Percent_Identity=24.7916666666667, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1788743, Length=461, Percent_Identity=31.8872017353579, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI226510925, Length=242, Percent_Identity=32.2314049586777, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786895, Length=171, Percent_Identity=30.4093567251462, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=482, Percent_Identity=27.5933609958506, Blast_Score=141, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6324540, Length=485, Percent_Identity=29.0721649484536, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6321192, Length=237, Percent_Identity=23.6286919831224, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24665321, Length=485, Percent_Identity=28.2474226804124, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24649466, Length=253, Percent_Identity=26.8774703557312, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_EHRRG (Q5FGB6)

Other databases:

- EMBL:   CR925677
- RefSeq:   YP_196719.1
- ProteinModelPortal:   Q5FGB6
- SMR:   Q5FGB6
- STRING:   Q5FGB6
- GeneID:   3268758
- GenomeReviews:   CR925677_GR
- KEGG:   erg:ERGA_CDS_07930
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   PHWRFKL
- ProtClustDB:   PRK12558
- BioCyc:   ERUM302409:ERGA_CDS_07930-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51820; Mature: 51820

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 241-241

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMMTRFAPSPTGYLHVGNVRTALICWLYTRSKQGRFLLRFDDTDLQRSKDDYRNEIAND
CEEEEECCCCCCCEEEECCHHEEEEEEEEECCCCCCEEEEECCCHHHCCHHHHHHHHHHC
LKWLQMDWDFDVRQSSRFDRYDEIFNYLLKEELIYPCYESKEELDFKRKMQLKLGLPPIY
CEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCHHHHEEEEECCCCCC
DRSALKLTQDEKNKYSEQDVYFRFKIDQSQLISWDDEIRGKVSFNAANISDPIVKRADGT
CCCCEEEECHHHHCCCCCCEEEEEEECHHHHCCCCHHCCCEEEECCCCCCCHHHHCCCCC
YTYMLPSVIDDIDFDITHIVRGEDHISNTAIQIQMFNALRASVPTFSHLSLLYCDDNKIS
EEEECHHHHCCCCCCEEEEECCCCCCCCCEEEEEEHHHHHHCCCCCCCEEEEEECCCHHH
KRVGGFSIKDMQFYELEPMAINSYFAKIGTSDPIVVHTKIQDLIYNFDITKFNQAPTQFN
HHHCCCEECCEEEEECCCEEHHHHHHHCCCCCCEEEEEEHHHHHHCCCCEECCCCCCCCC
IDDVIKLNPKVLHKMSFSDVKHRLSELNITSPDLWDFVSGNVQKFSDIQEWIKICGQSTV
CCHHEECCHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCC
PVINESDQDFIKMALSVFPNGEINQDTWKTWVANIKEKTDRKSKDIFIPLRLALTGISTG
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCC
PELAKLLPILGRAEIIRRLGYSSRC
HHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIMMTRFAPSPTGYLHVGNVRTALICWLYTRSKQGRFLLRFDDTDLQRSKDDYRNEIAND
CEEEEECCCCCCCEEEECCHHEEEEEEEEECCCCCCEEEEECCCHHHCCHHHHHHHHHHC
LKWLQMDWDFDVRQSSRFDRYDEIFNYLLKEELIYPCYESKEELDFKRKMQLKLGLPPIY
CEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCHHHHEEEEECCCCCC
DRSALKLTQDEKNKYSEQDVYFRFKIDQSQLISWDDEIRGKVSFNAANISDPIVKRADGT
CCCCEEEECHHHHCCCCCCEEEEEEECHHHHCCCCHHCCCEEEECCCCCCCHHHHCCCCC
YTYMLPSVIDDIDFDITHIVRGEDHISNTAIQIQMFNALRASVPTFSHLSLLYCDDNKIS
EEEECHHHHCCCCCCEEEEECCCCCCCCCEEEEEEHHHHHHCCCCCCCEEEEEECCCHHH
KRVGGFSIKDMQFYELEPMAINSYFAKIGTSDPIVVHTKIQDLIYNFDITKFNQAPTQFN
HHHCCCEECCEEEEECCCEEHHHHHHHCCCCCCEEEEEEHHHHHHCCCCEECCCCCCCCC
IDDVIKLNPKVLHKMSFSDVKHRLSELNITSPDLWDFVSGNVQKFSDIQEWIKICGQSTV
CCHHEECCHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCC
PVINESDQDFIKMALSVFPNGEINQDTWKTWVANIKEKTDRKSKDIFIPLRLALTGISTG
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCC
PELAKLLPILGRAEIIRRLGYSSRC
HHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA