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The map label for this gene is rpoA

Identifier: 58617333

GI number: 58617333

Start: 981756

End: 982880

Strand: Reverse

Name: rpoA

Synonym: ERGA_CDS_06060

Alternate gene names: 58617333

Gene position: 982880-981756 (Counterclockwise)

Preceding gene: 58617334

Following gene: 58617332

Centisome position: 65.53

GC content: 33.69

Gene sequence:

>1125_bases
GTGATTTTTGATGAGGATTCTAGTTCTGTTGCTCAAGAATCGGGTTATATGGGAGTAGGTAGTCCATACTCTAGTGATGT
AGGGCGTATATCAATGAGTGACCACTGGAATAAGTTAACTAAGCCTTCTTCTATTAAAGTAGTTAATCATGGTAATGCTT
TAAATAAGGCAGATTTAATTATTGAGCCACTTGAAAGCGGTTTTGCATTAACTCTAGGTAATGCTTTGAGAAGAGTTATG
ATGTCTTCTTTGCGTGGCTTTGCAGTTTATGGTGTTGAAATAGAAAATGTTTTGCATGAATTTACTTCAATTTCTGGGGT
TAGAGAGGATGTAACTGATATTCTATTGAATATAAGTATGATTAGGTTAAAGCTTTCTGGGATGGATAATAAGGTGTTAT
CTCTTAAAGTTAAGGGGCCTTGTGAAGTAAGGTCTGGAATGATAGCTGATACTCCTGATTGTACTATCTTAAATAAGGAT
TTATTGATTTGTACATTGGATCAGGATGTTGATTTTAATATAAAAATGTATGTTAATTCTGGAAAAGGATATGTTCCCGC
TGTGAAGCGTAAGTCAATTAATAAGTTTGGATTAAGTGATGTTCCTGTAAATTTTATTGCTACTAATGCTCTTTATAGTC
CGATCAAGAAAGCGTCTTTCAGAGTTGAGAGCAGTCGTATTGGTCAGTTTACAGATTATGATAGGTTGATTATGTCTGTA
GAGACAGATCATTCTATTTTACCTGATGAAGCTGTTGCATTGGCTGCAAGAATTTTGCAAGATCAGTTTCAACAGTTTAT
TAATTTTGATGAAACTGATGAGCCTCATCAGAAGGTTGATACTAAGGATGTATTACCTTATGATTCTAATTTATTACGTA
AGGTTGATGAGTTGGAGCTTTCGGTTAGATCATATAATTGCTTGAAAAATGATAATATTACTTATATAGGAGATTTAGTA
CAGAAGACTGAATCTGATATGTTAAGAACTCCAAATTTTGGTAGGAAGTCACTTAATGAAATTAATGAGCTTTTGGCAAG
TATGAATTTGCATTTGGGTATGAAGATTGCAAATTGGCCTCCTGAGTCCATTGAGAGTTTAAGTAAGCAATATAGTGAAG
AATAA

Upstream 100 bases:

>100_bases
ACTTCTATTAAAGATACTACGAAGCTTCCACATAATGGATGTAAGCTTCCAGGGAGGCGCAGGGTTTAATTATGTTTATA
GTAATGTGTGAGGTTCTATT

Downstream 100 bases:

>100_bases
GAAAAGTGAGGTTATGTTATGAGGCATCGTGTTGCTCATAGAAAGTTTTCTCGTACTAGTGCTCATAGAATCTCTATGTT
ATTAAATTTATCTATATCTT

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MIFDEDSSSVAQESGYMGVGSPYSSDVGRISMSDHWNKLTKPSSIKVVNHGNALNKADLIIEPLESGFALTLGNALRRVM
MSSLRGFAVYGVEIENVLHEFTSISGVREDVTDILLNISMIRLKLSGMDNKVLSLKVKGPCEVRSGMIADTPDCTILNKD
LLICTLDQDVDFNIKMYVNSGKGYVPAVKRKSINKFGLSDVPVNFIATNALYSPIKKASFRVESSRIGQFTDYDRLIMSV
ETDHSILPDEAVALAARILQDQFQQFINFDETDEPHQKVDTKDVLPYDSNLLRKVDELELSVRSYNCLKNDNITYIGDLV
QKTESDMLRTPNFGRKSLNEINELLASMNLHLGMKIANWPPESIESLSKQYSEE

Sequences:

>Translated_374_residues
MIFDEDSSSVAQESGYMGVGSPYSSDVGRISMSDHWNKLTKPSSIKVVNHGNALNKADLIIEPLESGFALTLGNALRRVM
MSSLRGFAVYGVEIENVLHEFTSISGVREDVTDILLNISMIRLKLSGMDNKVLSLKVKGPCEVRSGMIADTPDCTILNKD
LLICTLDQDVDFNIKMYVNSGKGYVPAVKRKSINKFGLSDVPVNFIATNALYSPIKKASFRVESSRIGQFTDYDRLIMSV
ETDHSILPDEAVALAARILQDQFQQFINFDETDEPHQKVDTKDVLPYDSNLLRKVDELELSVRSYNCLKNDNITYIGDLV
QKTESDMLRTPNFGRKSLNEINELLASMNLHLGMKIANWPPESIESLSKQYSEE
>Mature_374_residues
MIFDEDSSSVAQESGYMGVGSPYSSDVGRISMSDHWNKLTKPSSIKVVNHGNALNKADLIIEPLESGFALTLGNALRRVM
MSSLRGFAVYGVEIENVLHEFTSISGVREDVTDILLNISMIRLKLSGMDNKVLSLKVKGPCEVRSGMIADTPDCTILNKD
LLICTLDQDVDFNIKMYVNSGKGYVPAVKRKSINKFGLSDVPVNFIATNALYSPIKKASFRVESSRIGQFTDYDRLIMSV
ETDHSILPDEAVALAARILQDQFQQFINFDETDEPHQKVDTKDVLPYDSNLLRKVDELELSVRSYNCLKNDNITYIGDLV
QKTESDMLRTPNFGRKSLNEINELLASMNLHLGMKIANWPPESIESLSKQYSEE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=309, Percent_Identity=42.7184466019417, Blast_Score=263, Evalue=1e-71,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_EHRRG (Q5FFS1)

Other databases:

- EMBL:   CR925677
- RefSeq:   YP_196532.1
- ProteinModelPortal:   Q5FFS1
- STRING:   Q5FFS1
- GeneID:   3268113
- GenomeReviews:   CR925677_GR
- KEGG:   erg:ERGA_CDS_06060
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   ERUM302409:ERGA_CDS_06060-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 41808; Mature: 41808

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFDEDSSSVAQESGYMGVGSPYSSDVGRISMSDHWNKLTKPSSIKVVNHGNALNKADLI
CCCCCCCHHHHHHCCCCCCCCCCCCCCCEEECCHHHHHHCCCCEEEEEECCCCCCCCHHE
IEPLESGFALTLGNALRRVMMSSLRGFAVYGVEIENVLHEFTSISGVREDVTDILLNISM
EEEHHCCCEEHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHH
IRLKLSGMDNKVLSLKVKGPCEVRSGMIADTPDCTILNKDLLICTLDQDVDFNIKMYVNS
EEEEECCCCCEEEEEEECCCHHHHCCCCCCCCCCEEECCCEEEEEECCCCCEEEEEEEEC
GKGYVPAVKRKSINKFGLSDVPVNFIATNALYSPIKKASFRVESSRIGQFTDYDRLIMSV
CCCCCCHHHHCCCCCCCCCCCCHHHEEHHHHHHHHHHHHEEECHHHCCCCCCHHHEEEEE
ETDHSILPDEAVALAARILQDQFQQFINFDETDEPHQKVDTKDVLPYDSNLLRKVDELEL
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHH
SVRSYNCLKNDNITYIGDLVQKTESDMLRTPNFGRKSLNEINELLASMNLHLGMKIANWP
HHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHCCEEECCCC
PESIESLSKQYSEE
HHHHHHHHHHHCCC
>Mature Secondary Structure
MIFDEDSSSVAQESGYMGVGSPYSSDVGRISMSDHWNKLTKPSSIKVVNHGNALNKADLI
CCCCCCCHHHHHHCCCCCCCCCCCCCCCEEECCHHHHHHCCCCEEEEEECCCCCCCCHHE
IEPLESGFALTLGNALRRVMMSSLRGFAVYGVEIENVLHEFTSISGVREDVTDILLNISM
EEEHHCCCEEHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHH
IRLKLSGMDNKVLSLKVKGPCEVRSGMIADTPDCTILNKDLLICTLDQDVDFNIKMYVNS
EEEEECCCCCEEEEEEECCCHHHHCCCCCCCCCCEEECCCEEEEEECCCCCEEEEEEEEC
GKGYVPAVKRKSINKFGLSDVPVNFIATNALYSPIKKASFRVESSRIGQFTDYDRLIMSV
CCCCCCHHHHCCCCCCCCCCCCHHHEEHHHHHHHHHHHHEEECHHHCCCCCCHHHEEEEE
ETDHSILPDEAVALAARILQDQFQQFINFDETDEPHQKVDTKDVLPYDSNLLRKVDELEL
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHH
SVRSYNCLKNDNITYIGDLVQKTESDMLRTPNFGRKSLNEINELLASMNLHLGMKIANWP
HHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHCCEEECCCC
PESIESLSKQYSEE
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA