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The map label for this gene is mraW

Identifier: 58617225

GI number: 58617225

Start: 803665

End: 804570

Strand: Reverse

Name: mraW

Synonym: ERGA_CDS_04980

Alternate gene names: 58617225

Gene position: 804570-803665 (Counterclockwise)

Preceding gene: 58617226

Following gene: 58617222

Centisome position: 53.64

GC content: 30.46

Gene sequence:

>906_bases
ATGTACCATACTCCTGTTTTATTGAAGGAAATGTTAGATATATTGTCTCCTCAAAATGGAGGGATATATGTTGATGCAAC
ATTTGGTTCAGGAGGATATAGTAGGGCGATTTTAAATTCTGCAGATTGTCAAGTTTATGCTATTGATCAGGATGAATATA
CTTATACTTTTTATGAAAAGTTATCTCATGATTTTCCTAATAGAATACATTTCTTTATTAATAAATTTAGTAAAATACAG
CAAATATTGAATAATGTGCAGATAAAAAAGGTAGATGGTGTAGTATTTGATATAGGGGTGTCTTCTATGCAATTGGAAGA
TGCTAGTCGTGGGTTTTCTTTTTCTAAGAATGGGCCTCTTGATATGAGAATGAGTACCTCATTGTCTGGTGTTGATGCAC
GTATGTTTGTGAATACAGTATCTGAAGTAGAAATAGCTAATGTTATATATCAATATGGAGGAGAAAAATATTCACGAAAA
ATTGCAAGAGCTATTGTTAATGCTAGAAATAAAAATATGATTAATACTACAGGAGAACTTGCATCAATAATACGTTCTGT
GGTATCGCGTAGTAAAAACCATAGTATTGATCCAGCTACAAGAACTTTTCAAGCAATTAGGATATGGGTGAATAAAGAAT
TAGAGGAATTAGAGAAAGGTATAGCTTGTGCTGCAAATATATTAAATCAGGGTGGAAAAATAATAGTAATATCTTTTCAT
TCTTTAGAAGATAGAATTGTTAAAGTCATGTTTAAGTTATTGTGTGACGGAAAATCTGTCAATTTATTAAATTTAGGGTT
AGGATTTCAGTTAATTAATAAAAAAATAATTAGACCAACTGCTGAAGAAATACATAGTAATCCTAGAGCTAGATCTGCAA
AATTGCGTGCTATATTAAAACTATGA

Upstream 100 bases:

>100_bases
GTGTTATTGCTGTGGTTTTATAATGGAAATGTGTAGTGAAAAATGGTTTTTTATGTTAAAAGTGTAAGAGTAATAAAATT
TTTTCAGAGTAGATTAAAGA

Downstream 100 bases:

>100_bases
GAGTTTATTAAATATGATTTGCTAACTATATTGTTTCTTTATAGTAAATCTTGGAAGTTTTATAGTTGTCAATTAAATAA
TATTTGACAAATACGTTAGT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MYHTPVLLKEMLDILSPQNGGIYVDATFGSGGYSRAILNSADCQVYAIDQDEYTYTFYEKLSHDFPNRIHFFINKFSKIQ
QILNNVQIKKVDGVVFDIGVSSMQLEDASRGFSFSKNGPLDMRMSTSLSGVDARMFVNTVSEVEIANVIYQYGGEKYSRK
IARAIVNARNKNMINTTGELASIIRSVVSRSKNHSIDPATRTFQAIRIWVNKELEELEKGIACAANILNQGGKIIVISFH
SLEDRIVKVMFKLLCDGKSVNLLNLGLGFQLINKKIIRPTAEEIHSNPRARSAKLRAILKL

Sequences:

>Translated_301_residues
MYHTPVLLKEMLDILSPQNGGIYVDATFGSGGYSRAILNSADCQVYAIDQDEYTYTFYEKLSHDFPNRIHFFINKFSKIQ
QILNNVQIKKVDGVVFDIGVSSMQLEDASRGFSFSKNGPLDMRMSTSLSGVDARMFVNTVSEVEIANVIYQYGGEKYSRK
IARAIVNARNKNMINTTGELASIIRSVVSRSKNHSIDPATRTFQAIRIWVNKELEELEKGIACAANILNQGGKIIVISFH
SLEDRIVKVMFKLLCDGKSVNLLNLGLGFQLINKKIIRPTAEEIHSNPRARSAKLRAILKL
>Mature_301_residues
MYHTPVLLKEMLDILSPQNGGIYVDATFGSGGYSRAILNSADCQVYAIDQDEYTYTFYEKLSHDFPNRIHFFINKFSKIQ
QILNNVQIKKVDGVVFDIGVSSMQLEDASRGFSFSKNGPLDMRMSTSLSGVDARMFVNTVSEVEIANVIYQYGGEKYSRK
IARAIVNARNKNMINTTGELASIIRSVVSRSKNHSIDPATRTFQAIRIWVNKELEELEKGIACAANILNQGGKIIVISFH
SLEDRIVKVMFKLLCDGKSVNLLNLGLGFQLINKKIIRPTAEEIHSNPRARSAKLRAILKL

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=336, Percent_Identity=39.8809523809524, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI165377202, Length=187, Percent_Identity=42.7807486631016, Blast_Score=150, Evalue=1e-36,
Organism=Escherichia coli, GI1786270, Length=306, Percent_Identity=41.8300653594771, Blast_Score=216, Evalue=1e-57,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=38.0664652567976, Blast_Score=204, Evalue=8e-53,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=38.0664652567976, Blast_Score=204, Evalue=8e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_EHRRG (Q5FH93)

Other databases:

- EMBL:   CR925677
- RefSeq:   YP_196424.1
- ProteinModelPortal:   Q5FH93
- SMR:   Q5FH93
- STRING:   Q5FH93
- GeneID:   3268501
- GenomeReviews:   CR925677_GR
- KEGG:   erg:ERGA_CDS_04980
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   ERUM302409:ERGA_CDS_04980-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 33759; Mature: 33759

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: BINDING 49-49 BINDING 76-76 BINDING 97-97 BINDING 104-104

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYHTPVLLKEMLDILSPQNGGIYVDATFGSGGYSRAILNSADCQVYAIDQDEYTYTFYEK
CCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHCCCCCCEEEEECCCCEEHHHHHH
LSHDFPNRIHFFINKFSKIQQILNNVQIKKVDGVVFDIGVSSMQLEDASRGFSFSKNGPL
HCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCEEEECCCCCEEECHHHCCCCCCCCCCC
DMRMSTSLSGVDARMFVNTVSEVEIANVIYQYGGEKYSRKIARAIVNARNKNMINTTGEL
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHH
ASIIRSVVSRSKNHSIDPATRTFQAIRIWVNKELEELEKGIACAANILNQGGKIIVISFH
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
SLEDRIVKVMFKLLCDGKSVNLLNLGLGFQLINKKIIRPTAEEIHSNPRARSAKLRAILK
CHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHC
L
C
>Mature Secondary Structure
MYHTPVLLKEMLDILSPQNGGIYVDATFGSGGYSRAILNSADCQVYAIDQDEYTYTFYEK
CCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHCCCCCCEEEEECCCCEEHHHHHH
LSHDFPNRIHFFINKFSKIQQILNNVQIKKVDGVVFDIGVSSMQLEDASRGFSFSKNGPL
HCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCEEEECCCCCEEECHHHCCCCCCCCCCC
DMRMSTSLSGVDARMFVNTVSEVEIANVIYQYGGEKYSRKIARAIVNARNKNMINTTGEL
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHH
ASIIRSVVSRSKNHSIDPATRTFQAIRIWVNKELEELEKGIACAANILNQGGKIIVISFH
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
SLEDRIVKVMFKLLCDGKSVNLLNLGLGFQLINKKIIRPTAEEIHSNPRARSAKLRAILK
CHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA