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The map label for this gene is argG

Identifier: 58617112

GI number: 58617112

Start: 637938

End: 639122

Strand: Reverse

Name: argG

Synonym: ERGA_CDS_03850

Alternate gene names: 58617112

Gene position: 639122-637938 (Counterclockwise)

Preceding gene: 58617117

Following gene: 58617108

Centisome position: 42.61

GC content: 32.24

Gene sequence:

>1185_bases
ATGAAAAAAATAGTTCTAGCTTATTCTGGTGGGTTAGATACTTCTGTTATATTAAAATGGTTACAGGAAAATTATAATTG
TGAAGTAGTAGTATTTACCGCAGATATAGGACAAGAAGACGACATGTCTGTTATTCAAAAAAAAGCAGCTGCGTTAAATG
TAAAGGAAATTTTTATAGAAGATTTAAAGGAAGAATTTGTTAGAGATTTTGTATTCCCTATGTTTAGAGCAAACACTATA
TATGAGGGATATTACCTACTTGGAACATCTATAGCAAGACCATTAATAGCCAAAAGACAAATTGAAATAGCTCATCTTAC
AGGAGCAGATGCTGTAGCACATGGTGCAACTGGAAAAGGAAACGACCAAGTAAGATTTGAGTTTGGATATTATTGTTGTG
ATCCAAACATAAAAGTAATTGCTCCTTGGCGTCAGTGGGAATTAACATCAAGACACAGTCTTATAGAATATGCAAGAAAA
AATAACATAAATGTTCCTTTAGATAAAGTAAATGAACCACCATATTCAATAGATGCAAATTTATTACATATCTCATACGA
AGGAAAGTCTCTTGAAGATCCATATGTTGAACCAGATTATACTATGCTTTCCAGATCATTGACACCAGAGCTAGCAAGTA
GTATTCCGGAATACATAGAAATCACCTTTGAACAAGGAGATCCAATAGCAATCAATGACATACCACTTTCACCAGCGAAT
TTATTACATCAATTAAATAAGATCGGTGGAAAACATGGTATAGGAATTGTTGACATAGTAGAAAATCGTTACATAGGAAT
AAAATCAAGGGGAATATATGAAACTCCTGGAGGAACAATTCTTCTACATGCACATCGTGCTATAGAAAGCATAACATTAG
ATAGAGAATCTGCTCATCTTAAAGATGAGATTATGCCAAAATATGCAAAGTTAATATATAATGGTTATTGGTGGACTACT
GAAAGGAAAATGCTACAATCATTAATAGATAATCACAAGAAAAAATTTAATGGAACTGTACGCATCAAACTATATAAGGG
ATCAGTTGTAGTTGTAGGCCGTAAATCTAATAATAGCTTATATTCATATAATTTAGCAAGCTTTGACAGTGAATCTCAGG
GTTACGATCACAAAGACGCAGAAGGATTTATAAAAGTTAATTCTTTAAGGTTAAAAAAATCTTAA

Upstream 100 bases:

>100_bases
AACCTAATAAATTGTTAGATACAGGCTAATAATAAAATTAAGTTGTCATAATAATAAAAATACAATATATGTTACGTGTG
TATTTTTATTTTATTTATTT

Downstream 100 bases:

>100_bases
TTTATTTCTACTATTTTTACATCCTGTACTATACATCTGAATACACAAAAAACATTATATTAAGTAGCAAAACGTAACAC
TTAAATAACTTAAAGCTTAA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKKIVLAYSGGLDTSVILKWLQENYNCEVVVFTADIGQEDDMSVIQKKAAALNVKEIFIEDLKEEFVRDFVFPMFRANTI
YEGYYLLGTSIARPLIAKRQIEIAHLTGADAVAHGATGKGNDQVRFEFGYYCCDPNIKVIAPWRQWELTSRHSLIEYARK
NNINVPLDKVNEPPYSIDANLLHISYEGKSLEDPYVEPDYTMLSRSLTPELASSIPEYIEITFEQGDPIAINDIPLSPAN
LLHQLNKIGGKHGIGIVDIVENRYIGIKSRGIYETPGGTILLHAHRAIESITLDRESAHLKDEIMPKYAKLIYNGYWWTT
ERKMLQSLIDNHKKKFNGTVRIKLYKGSVVVVGRKSNNSLYSYNLASFDSESQGYDHKDAEGFIKVNSLRLKKS

Sequences:

>Translated_394_residues
MKKIVLAYSGGLDTSVILKWLQENYNCEVVVFTADIGQEDDMSVIQKKAAALNVKEIFIEDLKEEFVRDFVFPMFRANTI
YEGYYLLGTSIARPLIAKRQIEIAHLTGADAVAHGATGKGNDQVRFEFGYYCCDPNIKVIAPWRQWELTSRHSLIEYARK
NNINVPLDKVNEPPYSIDANLLHISYEGKSLEDPYVEPDYTMLSRSLTPELASSIPEYIEITFEQGDPIAINDIPLSPAN
LLHQLNKIGGKHGIGIVDIVENRYIGIKSRGIYETPGGTILLHAHRAIESITLDRESAHLKDEIMPKYAKLIYNGYWWTT
ERKMLQSLIDNHKKKFNGTVRIKLYKGSVVVVGRKSNNSLYSYNLASFDSESQGYDHKDAEGFIKVNSLRLKKS
>Mature_394_residues
MKKIVLAYSGGLDTSVILKWLQENYNCEVVVFTADIGQEDDMSVIQKKAAALNVKEIFIEDLKEEFVRDFVFPMFRANTI
YEGYYLLGTSIARPLIAKRQIEIAHLTGADAVAHGATGKGNDQVRFEFGYYCCDPNIKVIAPWRQWELTSRHSLIEYARK
NNINVPLDKVNEPPYSIDANLLHISYEGKSLEDPYVEPDYTMLSRSLTPELASSIPEYIEITFEQGDPIAINDIPLSPAN
LLHQLNKIGGKHGIGIVDIVENRYIGIKSRGIYETPGGTILLHAHRAIESITLDRESAHLKDEIMPKYAKLIYNGYWWTT
ERKMLQSLIDNHKKKFNGTVRIKLYKGSVVVVGRKSNNSLYSYNLASFDSESQGYDHKDAEGFIKVNSLRLKKS

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=399, Percent_Identity=45.6140350877193, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI16950633, Length=399, Percent_Identity=45.6140350877193, Blast_Score=350, Evalue=2e-96,
Organism=Escherichia coli, GI1789563, Length=367, Percent_Identity=28.3378746594005, Blast_Score=130, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324514, Length=406, Percent_Identity=40.8866995073892, Blast_Score=312, Evalue=5e-86,
Organism=Drosophila melanogaster, GI21358151, Length=405, Percent_Identity=42.4691358024691, Blast_Score=321, Evalue=6e-88,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_EHRRG (Q5FH27)

Other databases:

- EMBL:   CR925677
- RefSeq:   YP_196311.1
- HSSP:   P59846
- ProteinModelPortal:   Q5FH27
- SMR:   Q5FH27
- STRING:   Q5FH27
- GeneID:   3268229
- GenomeReviews:   CR925677_GR
- KEGG:   erg:ERGA_CDS_03850
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- ProtClustDB:   PRK00509
- BioCyc:   ERUM302409:ERGA_CDS_03850-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44674; Mature: 44674

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 34-34 BINDING 85-85 BINDING 90-90 BINDING 115-115 BINDING 117-117 BINDING 121-121 BINDING 121-121 BINDING 122-122 BINDING 125-125 BINDING 176-176 BINDING 185-185 BINDING 261-261 BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVLAYSGGLDTSVILKWLQENYNCEVVVFTADIGQEDDMSVIQKKAAALNVKEIFIE
CCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHH
DLKEEFVRDFVFPMFRANTIYEGYYLLGTSIARPLIAKRQIEIAHLTGADAVAHGATGKG
HHHHHHHHHHHHHHHHCCEEECCEEEECHHHHHHHHHHCEEEEEEECCCCHHHCCCCCCC
NDQVRFEFGYYCCDPNIKVIAPWRQWELTSRHSLIEYARKNNINVPLDKVNEPPYSIDAN
CCEEEEEEEEEEECCCCEEEECCHHCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCEECCE
LLHISYEGKSLEDPYVEPDYTMLSRSLTPELASSIPEYIEITFEQGDPIAINDIPLSPAN
EEEEEECCCCCCCCCCCCCHHHHHHCCCHHHHHCCCCEEEEEEECCCCEEEECCCCCHHH
LLHQLNKIGGKHGIGIVDIVENRYIGIKSRGIYETPGGTILLHAHRAIESITLDRESAHL
HHHHHHHCCCCCCCEEEEEECCCEEEEECCCEEECCCCEEEEEEHHHHHHEECCCCHHHH
KDEIMPKYAKLIYNGYWWTTERKMLQSLIDNHKKKFNGTVRIKLYKGSVVVVGRKSNNSL
HHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEEECCCCCE
YSYNLASFDSESQGYDHKDAEGFIKVNSLRLKKS
EEEEECCCCCCCCCCCCCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MKKIVLAYSGGLDTSVILKWLQENYNCEVVVFTADIGQEDDMSVIQKKAAALNVKEIFIE
CCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCHHHHHHH
DLKEEFVRDFVFPMFRANTIYEGYYLLGTSIARPLIAKRQIEIAHLTGADAVAHGATGKG
HHHHHHHHHHHHHHHHCCEEECCEEEECHHHHHHHHHHCEEEEEEECCCCHHHCCCCCCC
NDQVRFEFGYYCCDPNIKVIAPWRQWELTSRHSLIEYARKNNINVPLDKVNEPPYSIDAN
CCEEEEEEEEEEECCCCEEEECCHHCCCHHHHHHHHHHHHCCCCCCHHHCCCCCCEECCE
LLHISYEGKSLEDPYVEPDYTMLSRSLTPELASSIPEYIEITFEQGDPIAINDIPLSPAN
EEEEEECCCCCCCCCCCCCHHHHHHCCCHHHHHCCCCEEEEEEECCCCEEEECCCCCHHH
LLHQLNKIGGKHGIGIVDIVENRYIGIKSRGIYETPGGTILLHAHRAIESITLDRESAHL
HHHHHHHCCCCCCCEEEEEECCCEEEEECCCEEECCCCEEEEEEHHHHHHEECCCCHHHH
KDEIMPKYAKLIYNGYWWTTERKMLQSLIDNHKKKFNGTVRIKLYKGSVVVVGRKSNNSL
HHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEEECCCCCE
YSYNLASFDSESQGYDHKDAEGFIKVNSLRLKKS
EEEEECCCCCCCCCCCCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA