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The map label for this gene is proS

Identifier: 58617074

GI number: 58617074

Start: 572642

End: 573922

Strand: Reverse

Name: proS

Synonym: ERGA_CDS_03470

Alternate gene names: 58617074

Gene position: 573922-572642 (Counterclockwise)

Preceding gene: 58617075

Following gene: 58617073

Centisome position: 38.26

GC content: 30.99

Gene sequence:

>1281_bases
ATGCGTTTATCAAAATATTATATACCCACATTAAAAGAAACACCAACTGATGTATCAGTAACATCACATGTGTATTCTCT
ACGTTCAGGGTTAATACGACAAGTTGCATCTGGTATATATGCATGGCTTCCTCTAGGATTAAAAGTATTAAAAAATATAG
AGAATGTTATAAAAGAAGAAATGAATAAATCAGGAATTCTTGAAGTATTAATGCCACTAATCCAACCAGCATCACTATGG
CAAGAATCTGGAAGATACAATGACTATGGATCTGAAATGTTACGTATCAAAGATCGAAACAACCGAGAAATGGTATTTGG
CCCTACTCATGAAGAAGTTGTAACAGATCTATTAAGAACAACATTAACAAGCTATAAAAACTTACCACTTATTTTATATC
AAGTACAGTGGAAATTCAGAGATGAGTTACGTCCACGATATGGGATTATGAGATGCAGAGAATTTTTAATGAAAGATGCT
TACAGCTTTGATAAAGATTTTAATAGTGCAATTGAATCTTATAATTTAATGTTTAAAGTTTACATCCAAATTTTTAGGAG
ATTAGGATTAACCCCAATAGCTGTTAAAGCAGATTCTGGACCAATAGGAGGTAATTTAAGCCATGAGTTTCACATATTAG
CAAATTCTGGAGAAAGTACACTATATTACGATCAAGATATAATTAACTTAATTAACAATGACAATATTGATATTGAAAAA
ATAAAGAATACTTATACTGCTGCAGATGATTTACACAATTCAGAAACTTGCCCTATTCCAGCATCTAACATAAAAACAAG
TAAAGGTATAGAAATCGGTCATATATTTTATTTAGGTGATAAATACTCAAAAACAATGAACGCGGCATTCTCCCAGAATA
ATGAAACTCAGCTATTACATATGGGATGCTATGGTATAGGAGTGTCAAGATTAGTAGGTGCAATCATTGAGGTTTCCCAT
GATAATAATGGTATTATTTGGCCAGAAGAAATAGCACCATTTAAATTCTCTTTAGTAAACGTGTTTTCAGCAAATAAAGA
ATGTCGCAATATTTCAGAAAATTTATATGCAAAATTAGACAGCAATGATGTATTATACGATGACACAGAAGACAGTACAG
GTATTAAATTCTCAAGAATGGATTTATTAGGTATGCCATGGCAAGTTATTATTGGAAAAACAACAATAGAACATGGTCTC
ATAGAAGTTAGGCAAAGGTCTAGTAAGAAGTCATTATTAATGTCTACAGAACAGTTTCTTAATAATTTTAAAAAATCATA
A

Upstream 100 bases:

>100_bases
TTATATAACATGACTTAAAACATCATAAGAAAGCTTAACATTGACTATTAAACCTATATATGGTATTTTCCTACACTTAT
CTAACCAAAGTTAGTATTTC

Downstream 100 bases:

>100_bases
GATGATAGTAGGAATTGGTATAGACGTAGTTTATATCCCACGTATATTAAACTTATGGAAAAAGTTTGGTAATAAGTTTC
TGAAAAAAGTATTTTCACAA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MRLSKYYIPTLKETPTDVSVTSHVYSLRSGLIRQVASGIYAWLPLGLKVLKNIENVIKEEMNKSGILEVLMPLIQPASLW
QESGRYNDYGSEMLRIKDRNNREMVFGPTHEEVVTDLLRTTLTSYKNLPLILYQVQWKFRDELRPRYGIMRCREFLMKDA
YSFDKDFNSAIESYNLMFKVYIQIFRRLGLTPIAVKADSGPIGGNLSHEFHILANSGESTLYYDQDIINLINNDNIDIEK
IKNTYTAADDLHNSETCPIPASNIKTSKGIEIGHIFYLGDKYSKTMNAAFSQNNETQLLHMGCYGIGVSRLVGAIIEVSH
DNNGIIWPEEIAPFKFSLVNVFSANKECRNISENLYAKLDSNDVLYDDTEDSTGIKFSRMDLLGMPWQVIIGKTTIEHGL
IEVRQRSSKKSLLMSTEQFLNNFKKS

Sequences:

>Translated_426_residues
MRLSKYYIPTLKETPTDVSVTSHVYSLRSGLIRQVASGIYAWLPLGLKVLKNIENVIKEEMNKSGILEVLMPLIQPASLW
QESGRYNDYGSEMLRIKDRNNREMVFGPTHEEVVTDLLRTTLTSYKNLPLILYQVQWKFRDELRPRYGIMRCREFLMKDA
YSFDKDFNSAIESYNLMFKVYIQIFRRLGLTPIAVKADSGPIGGNLSHEFHILANSGESTLYYDQDIINLINNDNIDIEK
IKNTYTAADDLHNSETCPIPASNIKTSKGIEIGHIFYLGDKYSKTMNAAFSQNNETQLLHMGCYGIGVSRLVGAIIEVSH
DNNGIIWPEEIAPFKFSLVNVFSANKECRNISENLYAKLDSNDVLYDDTEDSTGIKFSRMDLLGMPWQVIIGKTTIEHGL
IEVRQRSSKKSLLMSTEQFLNNFKKS
>Mature_426_residues
MRLSKYYIPTLKETPTDVSVTSHVYSLRSGLIRQVASGIYAWLPLGLKVLKNIENVIKEEMNKSGILEVLMPLIQPASLW
QESGRYNDYGSEMLRIKDRNNREMVFGPTHEEVVTDLLRTTLTSYKNLPLILYQVQWKFRDELRPRYGIMRCREFLMKDA
YSFDKDFNSAIESYNLMFKVYIQIFRRLGLTPIAVKADSGPIGGNLSHEFHILANSGESTLYYDQDIINLINNDNIDIEK
IKNTYTAADDLHNSETCPIPASNIKTSKGIEIGHIFYLGDKYSKTMNAAFSQNNETQLLHMGCYGIGVSRLVGAIIEVSH
DNNGIIWPEEIAPFKFSLVNVFSANKECRNISENLYAKLDSNDVLYDDTEDSTGIKFSRMDLLGMPWQVIIGKTTIEHGL
IEVRQRSSKKSLLMSTEQFLNNFKKS

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=400, Percent_Identity=36.5, Blast_Score=265, Evalue=6e-71,
Organism=Escherichia coli, GI1786392, Length=223, Percent_Identity=58.2959641255605, Blast_Score=291, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI115532348, Length=417, Percent_Identity=33.3333333333333, Blast_Score=211, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI193203271, Length=111, Percent_Identity=32.4324324324324, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320931, Length=193, Percent_Identity=41.9689119170984, Blast_Score=169, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24656200, Length=409, Percent_Identity=31.7848410757946, Blast_Score=232, Evalue=4e-61,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_EHRRG (Q5FHL5)

Other databases:

- EMBL:   CR925677
- RefSeq:   YP_196273.1
- ProteinModelPortal:   Q5FHL5
- SMR:   Q5FHL5
- STRING:   Q5FHL5
- GeneID:   3268198
- GenomeReviews:   CR925677_GR
- KEGG:   erg:ERGA_CDS_03470
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK12325
- BioCyc:   ERUM302409:ERGA_CDS_03470-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 48667; Mature: 48667

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSKYYIPTLKETPTDVSVTSHVYSLRSGLIRQVASGIYAWLPLGLKVLKNIENVIKEE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNKSGILEVLMPLIQPASLWQESGRYNDYGSEMLRIKDRNNREMVFGPTHEEVVTDLLRT
HCCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHH
TLTSYKNLPLILYQVQWKFRDELRPRYGIMRCREFLMKDAYSFDKDFNSAIESYNLMFKV
HHHHHCCCCEEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
YIQIFRRLGLTPIAVKADSGPIGGNLSHEFHILANSGESTLYYDQDIINLINNDNIDIEK
HHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCEEEECHHHHHHHCCCCCCHHH
IKNTYTAADDLHNSETCPIPASNIKTSKGIEIGHIFYLGDKYSKTMNAAFSQNNETQLLH
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHCCCCCCEEEE
MGCYGIGVSRLVGAIIEVSHDNNGIIWPEEIAPFKFSLVNVFSANKECRNISENLYAKLD
ECHHHCCHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHEEEEC
SNDVLYDDTEDSTGIKFSRMDLLGMPWQVIIGKTTIEHGLIEVRQRSSKKSLLMSTEQFL
CCCEEECCCCCCCCCEEEEEHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
NNFKKS
HHHCCC
>Mature Secondary Structure
MRLSKYYIPTLKETPTDVSVTSHVYSLRSGLIRQVASGIYAWLPLGLKVLKNIENVIKEE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNKSGILEVLMPLIQPASLWQESGRYNDYGSEMLRIKDRNNREMVFGPTHEEVVTDLLRT
HCCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHH
TLTSYKNLPLILYQVQWKFRDELRPRYGIMRCREFLMKDAYSFDKDFNSAIESYNLMFKV
HHHHHCCCCEEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
YIQIFRRLGLTPIAVKADSGPIGGNLSHEFHILANSGESTLYYDQDIINLINNDNIDIEK
HHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCEEEECHHHHHHHCCCCCCHHH
IKNTYTAADDLHNSETCPIPASNIKTSKGIEIGHIFYLGDKYSKTMNAAFSQNNETQLLH
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHCCCCCCEEEE
MGCYGIGVSRLVGAIIEVSHDNNGIIWPEEIAPFKFSLVNVFSANKECRNISENLYAKLD
ECHHHCCHHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHEEEEC
SNDVLYDDTEDSTGIKFSRMDLLGMPWQVIIGKTTIEHGLIEVRQRSSKKSLLMSTEQFL
CCCEEECCCCCCCCCEEEEEHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
NNFKKS
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA