Click here to switch to the map view.

The map label for this gene is nifR3 [H]

Identifier: 58617063

GI number: 58617063

Start: 556780

End: 557733

Strand: Reverse

Name: nifR3 [H]

Synonym: ERGA_CDS_03360

Alternate gene names: 58617063

Gene position: 557733-556780 (Counterclockwise)

Preceding gene: 58617065

Following gene: 58617062

Centisome position: 37.18

GC content: 32.6

Gene sequence:

>954_bases
ATGAATTTGTCTCAATTTATTTCTGATTATCCTGTAATATTAGCACCTATGTCTGGTATAACTGATTTTCCATTTCGTGC
TCTTGTAAAAAACCTTGGAGCAGGATTATTAGTATCAGAAATGATAGCAAGCCGTGGAATGATCAAACAAACTAAAGATA
GTCTACAGAAGTCACATATACAATCTAATACTGCTGTGCAATTAGCTGGATGCGATCCACTAATAATGGCAGAAGCCGCA
AAACTTAATGAAGACATGGGTGCACAAATTATTGACATAAATTTTGGATGTCCTGTAAAAAAAGTAGTTAATAGCTATGC
TGGATCAGCACTAATGAAAGATGAAATAACAGCTGCAAAGATTTTAGAAGCTGTAGTAAAAGCAGTAAAAGTACCAGTAA
CATTAAAAATGCGTATTGGATGGGACAGTCAGAACCTAAATTCCCCTAGATTAGCAAAAATTGCTGAAAACACTGGGATT
AAAATGATCACTATACATGGTAGAACCAGAGCACAAATGTTTACCGGAAAAGCAGACTGGAAGTTCATCAGTAACATAAA
AAAATGTGTTAGAATTCCGATTATAGTCAATGGTGATATAAAAACTCTAGATGATATAAAAATAGCATTAGAAGAATCAC
AAGCTGATGGAATAATGATAGGAAGAGGTGCATATGGTAAACCATGGATTATAAATAAAGCACTACAATTTATAAAATCA
GGTATTATTTTACCAGATCCAACAGTATCGGAAAAATTACAAATTATTATAAATCACTACGACAACATCATAGAACATTA
TGGAAAGGACATTGGAGTTAAGATATCAAAAAAACATATAGGCTGGTACAGCGCTTGCCTAGAAAACTCTACAGAATTTA
GAAGTAAAGTCAACTGTTCAAACAATCATCAATTTATTAGAGATCAAATATTAAACTTTTTCTCACCAAAGTAA

Upstream 100 bases:

>100_bases
TTAAGTATATTTTCTTTAATTACATTTTAGTATTATGATTGATAATATTGTGGATCTATATCACAATTATTTAATAAACT
AGTTATACAACCTAACAAAT

Downstream 100 bases:

>100_bases
TCTTTTTTCTATTATTCAAATATTGATTATCTTTAAAATTATAATAATACATTTATATGTGATATTACATTGTGATGCTG
CATACAATAACTTTACACAT

Product: putative nitrogen regulation protein nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MNLSQFISDYPVILAPMSGITDFPFRALVKNLGAGLLVSEMIASRGMIKQTKDSLQKSHIQSNTAVQLAGCDPLIMAEAA
KLNEDMGAQIIDINFGCPVKKVVNSYAGSALMKDEITAAKILEAVVKAVKVPVTLKMRIGWDSQNLNSPRLAKIAENTGI
KMITIHGRTRAQMFTGKADWKFISNIKKCVRIPIIVNGDIKTLDDIKIALEESQADGIMIGRGAYGKPWIINKALQFIKS
GIILPDPTVSEKLQIIINHYDNIIEHYGKDIGVKISKKHIGWYSACLENSTEFRSKVNCSNNHQFIRDQILNFFSPK

Sequences:

>Translated_317_residues
MNLSQFISDYPVILAPMSGITDFPFRALVKNLGAGLLVSEMIASRGMIKQTKDSLQKSHIQSNTAVQLAGCDPLIMAEAA
KLNEDMGAQIIDINFGCPVKKVVNSYAGSALMKDEITAAKILEAVVKAVKVPVTLKMRIGWDSQNLNSPRLAKIAENTGI
KMITIHGRTRAQMFTGKADWKFISNIKKCVRIPIIVNGDIKTLDDIKIALEESQADGIMIGRGAYGKPWIINKALQFIKS
GIILPDPTVSEKLQIIINHYDNIIEHYGKDIGVKISKKHIGWYSACLENSTEFRSKVNCSNNHQFIRDQILNFFSPK
>Mature_317_residues
MNLSQFISDYPVILAPMSGITDFPFRALVKNLGAGLLVSEMIASRGMIKQTKDSLQKSHIQSNTAVQLAGCDPLIMAEAA
KLNEDMGAQIIDINFGCPVKKVVNSYAGSALMKDEITAAKILEAVVKAVKVPVTLKMRIGWDSQNLNSPRLAKIAENTGI
KMITIHGRTRAQMFTGKADWKFISNIKKCVRIPIIVNGDIKTLDDIKIALEESQADGIMIGRGAYGKPWIINKALQFIKS
GIILPDPTVSEKLQIIINHYDNIIEHYGKDIGVKISKKHIGWYSACLENSTEFRSKVNCSNNHQFIRDQILNFFSPK

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=283, Percent_Identity=30.3886925795053, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI239788462, Length=283, Percent_Identity=30.3886925795053, Blast_Score=131, Evalue=6e-31,
Organism=Homo sapiens, GI40807366, Length=223, Percent_Identity=27.3542600896861, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI31742496, Length=224, Percent_Identity=29.9107142857143, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI8923374, Length=279, Percent_Identity=29.3906810035842, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1789660, Length=301, Percent_Identity=42.1926910299003, Blast_Score=243, Evalue=8e-66,
Organism=Escherichia coli, GI1788462, Length=231, Percent_Identity=32.9004329004329, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI145693211, Length=231, Percent_Identity=31.6017316017316, Blast_Score=101, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17543114, Length=278, Percent_Identity=29.136690647482, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17507177, Length=229, Percent_Identity=27.9475982532751, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25144369, Length=221, Percent_Identity=29.8642533936652, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=288, Percent_Identity=27.7777777777778, Blast_Score=108, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6323560, Length=256, Percent_Identity=30.078125, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=235, Percent_Identity=28.5106382978723, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585320, Length=283, Percent_Identity=30.0353356890459, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24580595, Length=224, Percent_Identity=33.0357142857143, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI19920448, Length=224, Percent_Identity=33.0357142857143, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI45549423, Length=274, Percent_Identity=28.8321167883212, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921524, Length=271, Percent_Identity=26.9372693726937, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35087; Mature: 35087

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSQFISDYPVILAPMSGITDFPFRALVKNLGAGLLVSEMIASRGMIKQTKDSLQKSHI
CCHHHHHCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHC
QSNTAVQLAGCDPLIMAEAAKLNEDMGAQIIDINFGCPVKKVVNSYAGSALMKDEITAAK
CCCCEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHH
ILEAVVKAVKVPVTLKMRIGWDSQNLNSPRLAKIAENTGIKMITIHGRTRAQMFTGKADW
HHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHCCCEEEEEEECCCEEEEECCCCCH
KFISNIKKCVRIPIIVNGDIKTLDDIKIALEESQADGIMIGRGAYGKPWIINKALQFIKS
HHHHHHHHHHEECEEECCCCCCHHHHHEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHC
GIILPDPTVSEKLQIIINHYDNIIEHYGKDIGVKISKKHIGWYSACLENSTEFRSKVNCS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHCHHHHHHHCCCHHHHHHCCCC
NNHQFIRDQILNFFSPK
CCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLSQFISDYPVILAPMSGITDFPFRALVKNLGAGLLVSEMIASRGMIKQTKDSLQKSHI
CCHHHHHCCCCEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHC
QSNTAVQLAGCDPLIMAEAAKLNEDMGAQIIDINFGCPVKKVVNSYAGSALMKDEITAAK
CCCCEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHH
ILEAVVKAVKVPVTLKMRIGWDSQNLNSPRLAKIAENTGIKMITIHGRTRAQMFTGKADW
HHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHCCCEEEEEEECCCEEEEECCCCCH
KFISNIKKCVRIPIIVNGDIKTLDDIKIALEESQADGIMIGRGAYGKPWIINKALQFIKS
HHHHHHHHHHEECEEECCCCCCHHHHHEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHC
GIILPDPTVSEKLQIIINHYDNIIEHYGKDIGVKISKKHIGWYSACLENSTEFRSKVNCS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHCHHHHHHHCCCHHHHHHCCCC
NNHQFIRDQILNFFSPK
CCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA