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The map label for this gene is pleD [H]

Identifier: 58617050

GI number: 58617050

Start: 533315

End: 534700

Strand: Reverse

Name: pleD [H]

Synonym: ERGA_CDS_03230

Alternate gene names: 58617050

Gene position: 534700-533315 (Counterclockwise)

Preceding gene: 58617051

Following gene: 58617048

Centisome position: 35.65

GC content: 30.95

Gene sequence:

>1386_bases
ATGACTGCAAAAGTATTAATAGTAGATGATTTAGCTGCAAACATTAAGTTACTGCAAGCTAAACTAGTTAATGAATATTA
TAACGTATTGACTGCAACAAATGGCATGGATGCCATACAGATTGCTCAAGAGAAGCATCCTGATATAATATTACTAGATG
TGATGATGCCAGAAATGGATGGTTTCGAAACTTGCAAAAGATTAAAATCAGATCCTACAACCATCCATATTCCAGTAGTT
ATGGTAACAGCTCTTGACAACACTTCAGATAACAGAGCTACTGGTCTTAGTCATGGTGCTGATGATTTCTTGACAAAACC
TATAAATGACATTGCATTATTTGCAAGAATTAGGTCATTAACAAGATTCAAAATGGTAATAGATGAGTTAAGATTACGAT
GCAAAACAAACACTGAAATGAGTGTATTAAATGATAGTATAATGAACTATGCCAATAAAATAGATGATGCTAATATTTTA
ATAGCAGACGAAGATACATTAAGATCTGAACACATCAATAACATACTACAACAATCATTTCAACAAACTGTAGTCTCACA
CAATATAGAACAAGTTCTCAATTTAAGCAACATAGAGAATTACGATCTTATAATCATTGACTTAGAGTTTTCTGGAGACG
GATTAAGACTATGTTCACATTTACGTAACAAAATTGAAACACGTTATACACCTATTCTAGTCTTACTTGATGAAAATGAA
GACACAAAGCTACTTACTAAAGCATTTGATATAGGAATTCATGACTATATAACAACCCCAGTAGATAGTAACGAACTCAT
AGCAAGAGTTAATGTACAAGTAAAAAGAAAAAGATATCAAGATGCCTTAAGGATTAACGTAGATACTAATATGACAATGG
CAATAACTGATCCTCTTACTGGATGTTTTAATAGACGTTACTTTGACATGCATTTTCATAACATGATTACTGAATCATTA
TGTAAAGATAAGACTATATCTTTAATGATACTAGATATTGACCATTTCAAAGAAGTAAATGATACATTTGGTCATACCAT
AGGAGACGAATTACTACGACAATTTAAAAATAGAGTATCCGATAATATACGCATAACAGATTTATTAGCAAGATTTGGAG
GTGAAGAATTTGTTATAGTACTACCTGACACAGATATAAACACAGCATATCAAACAGGATTAAGAATACTCTCACTAGTA
GGAGAAACACCCTTTAAAACCAGCATAGGAGATATACACAAAACTGTTAGTATTGGTATCACAGAAATGTCACATTCTGA
TATTAATAACATAAAACAATTAATAGATAGAGCTGATAAATGTTTATATGAAGCAAAAAACACAGGAAGAAATAAAATTG
TAACTGACCCTATAATAAACACTTAA

Upstream 100 bases:

>100_bases
ATGATAACAACAATGTAGTTATTAAAAAAATAATAGGAAATGATGGTAAAACATACGATAGTTATGTAACAAAAAATAAT
CCCAAGAAAAAAGAAGACTT

Downstream 100 bases:

>100_bases
CATATAATTATTTCAAATGTAATAGTTAAATAATTACTCTACATTTTAATAATTTTTTGTATTATCTCATAACTAATAAC
ATATAGAATATCATAAACAC

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MTAKVLIVDDLAANIKLLQAKLVNEYYNVLTATNGMDAIQIAQEKHPDIILLDVMMPEMDGFETCKRLKSDPTTIHIPVV
MVTALDNTSDNRATGLSHGADDFLTKPINDIALFARIRSLTRFKMVIDELRLRCKTNTEMSVLNDSIMNYANKIDDANIL
IADEDTLRSEHINNILQQSFQQTVVSHNIEQVLNLSNIENYDLIIIDLEFSGDGLRLCSHLRNKIETRYTPILVLLDENE
DTKLLTKAFDIGIHDYITTPVDSNELIARVNVQVKRKRYQDALRINVDTNMTMAITDPLTGCFNRRYFDMHFHNMITESL
CKDKTISLMILDIDHFKEVNDTFGHTIGDELLRQFKNRVSDNIRITDLLARFGGEEFVIVLPDTDINTAYQTGLRILSLV
GETPFKTSIGDIHKTVSIGITEMSHSDINNIKQLIDRADKCLYEAKNTGRNKIVTDPIINT

Sequences:

>Translated_461_residues
MTAKVLIVDDLAANIKLLQAKLVNEYYNVLTATNGMDAIQIAQEKHPDIILLDVMMPEMDGFETCKRLKSDPTTIHIPVV
MVTALDNTSDNRATGLSHGADDFLTKPINDIALFARIRSLTRFKMVIDELRLRCKTNTEMSVLNDSIMNYANKIDDANIL
IADEDTLRSEHINNILQQSFQQTVVSHNIEQVLNLSNIENYDLIIIDLEFSGDGLRLCSHLRNKIETRYTPILVLLDENE
DTKLLTKAFDIGIHDYITTPVDSNELIARVNVQVKRKRYQDALRINVDTNMTMAITDPLTGCFNRRYFDMHFHNMITESL
CKDKTISLMILDIDHFKEVNDTFGHTIGDELLRQFKNRVSDNIRITDLLARFGGEEFVIVLPDTDINTAYQTGLRILSLV
GETPFKTSIGDIHKTVSIGITEMSHSDINNIKQLIDRADKCLYEAKNTGRNKIVTDPIINT
>Mature_460_residues
TAKVLIVDDLAANIKLLQAKLVNEYYNVLTATNGMDAIQIAQEKHPDIILLDVMMPEMDGFETCKRLKSDPTTIHIPVVM
VTALDNTSDNRATGLSHGADDFLTKPINDIALFARIRSLTRFKMVIDELRLRCKTNTEMSVLNDSIMNYANKIDDANILI
ADEDTLRSEHINNILQQSFQQTVVSHNIEQVLNLSNIENYDLIIIDLEFSGDGLRLCSHLRNKIETRYTPILVLLDENED
TKLLTKAFDIGIHDYITTPVDSNELIARVNVQVKRKRYQDALRINVDTNMTMAITDPLTGCFNRRYFDMHFHNMITESLC
KDKTISLMILDIDHFKEVNDTFGHTIGDELLRQFKNRVSDNIRITDLLARFGGEEFVIVLPDTDINTAYQTGLRILSLVG
ETPFKTSIGDIHKTVSIGITEMSHSDINNIKQLIDRADKCLYEAKNTGRNKIVTDPIINT

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=171, Percent_Identity=43.2748538011696, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=40.251572327044, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786584, Length=187, Percent_Identity=28.3422459893048, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=36.7088607594937, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87081881, Length=165, Percent_Identity=38.1818181818182, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788381, Length=163, Percent_Identity=33.1288343558282, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1787802, Length=208, Percent_Identity=29.3269230769231, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=31.055900621118, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788085, Length=167, Percent_Identity=32.9341317365269, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1787541, Length=187, Percent_Identity=29.9465240641711, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788956, Length=168, Percent_Identity=32.1428571428571, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786599, Length=132, Percent_Identity=35.6060606060606, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786784, Length=119, Percent_Identity=35.2941176470588, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789809, Length=121, Percent_Identity=37.1900826446281, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788394, Length=142, Percent_Identity=33.0985915492958, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87082012, Length=119, Percent_Identity=31.9327731092437, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI2367329, Length=119, Percent_Identity=42.8571428571429, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787056, Length=162, Percent_Identity=30.2469135802469, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790860, Length=120, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI145693157, Length=104, Percent_Identity=35.5769230769231, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1789149, Length=106, Percent_Identity=34.9056603773585, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI87081974, Length=156, Percent_Identity=27.5641025641026, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790437, Length=106, Percent_Identity=37.7358490566038, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788550, Length=111, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 52347; Mature: 52216

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKVLIVDDLAANIKLLQAKLVNEYYNVLTATNGMDAIQIAQEKHPDIILLDVMMPEMD
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEEECCCCC
GFETCKRLKSDPTTIHIPVVMVTALDNTSDNRATGLSHGADDFLTKPINDIALFARIRSL
CHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHH
TRFKMVIDELRLRCKTNTEMSVLNDSIMNYANKIDDANILIADEDTLRSEHINNILQQSF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHH
QQTVVSHNIEQVLNLSNIENYDLIIIDLEFSGDGLRLCSHLRNKIETRYTPILVLLDENE
HHHHHHHCHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCC
DTKLLTKAFDIGIHDYITTPVDSNELIARVNVQVKRKRYQDALRINVDTNMTMAITDPLT
CCHHHHHHHHCCHHHHCCCCCCCCCEEEEECHHHHHHHCCCCEEEEECCCEEEEECCCHH
GCFNRRYFDMHFHNMITESLCKDKTISLMILDIDHFKEVNDTFGHTIGDELLRQFKNRVS
HHHCCCEEEHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHC
DNIRITDLLARFGGEEFVIVLPDTDINTAYQTGLRILSLVGETPFKTSIGDIHKTVSIGI
CCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEEE
TEMSHSDINNIKQLIDRADKCLYEAKNTGRNKIVTDPIINT
EECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCC
>Mature Secondary Structure 
TAKVLIVDDLAANIKLLQAKLVNEYYNVLTATNGMDAIQIAQEKHPDIILLDVMMPEMD
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEEEECCCCC
GFETCKRLKSDPTTIHIPVVMVTALDNTSDNRATGLSHGADDFLTKPINDIALFARIRSL
CHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHH
TRFKMVIDELRLRCKTNTEMSVLNDSIMNYANKIDDANILIADEDTLRSEHINNILQQSF
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHH
QQTVVSHNIEQVLNLSNIENYDLIIIDLEFSGDGLRLCSHLRNKIETRYTPILVLLDENE
HHHHHHHCHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCC
DTKLLTKAFDIGIHDYITTPVDSNELIARVNVQVKRKRYQDALRINVDTNMTMAITDPLT
CCHHHHHHHHCCHHHHCCCCCCCCCEEEEECHHHHHHHCCCCEEEEECCCEEEEECCCHH
GCFNRRYFDMHFHNMITESLCKDKTISLMILDIDHFKEVNDTFGHTIGDELLRQFKNRVS
HHHCCCEEEHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHC
DNIRITDLLARFGGEEFVIVLPDTDINTAYQTGLRILSLVGETPFKTSIGDIHKTVSIGI
CCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEEE
TEMSHSDINNIKQLIDRADKCLYEAKNTGRNKIVTDPIINT
EECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA